ATE502115T1 - Verfahren zur produktion von hyaluronan in einer rekombinanten wirtszelle - Google Patents

Verfahren zur produktion von hyaluronan in einer rekombinanten wirtszelle

Info

Publication number: ATE502115T1
Authority: AT; Austria
Prior art keywords: udp; glucose; gene; host cell; hyaluronan synthase
Prior art date: 2001-12-21

Application number

AT02805661T

Other languages

English (en)

Inventor

Alan Sloma

Regine Behr

William Widner

Maria Tang

David Sternberg

Stephen Brown

Original Assignee

Novozymes Biopharma Dk As

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-12-21

Filing date

2002-12-20

Publication date

2011-04-15

2002-12-20 Application filed by Novozymes Biopharma Dk As filed Critical Novozymes Biopharma Dk As

2011-04-15 Application granted granted Critical

2011-04-15 Publication of ATE502115T1 publication Critical patent/ATE502115T1/de

Links

229920002674 hyaluronan Polymers 0.000 title abstract 4
238000004519 manufacturing process Methods 0.000 title abstract 2
KIUKXJAPPMFGSW-MNSSHETKSA-N hyaluronan Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)C1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H](C(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-MNSSHETKSA-N 0.000 title 1
229940099552 hyaluronan Drugs 0.000 title 1
108090000320 Hyaluronan Synthases Proteins 0.000 abstract 4
KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 abstract 3
102000003918 Hyaluronan Synthases Human genes 0.000 abstract 3
229960003160 hyaluronic acid Drugs 0.000 abstract 3
150000007523 nucleic acids Chemical class 0.000 abstract 3
241000193830 Bacillus <bacterium> Species 0.000 abstract 2
101710167959 Putative UTP-glucose-1-phosphate uridylyltransferase Proteins 0.000 abstract 2
108030001662 UDP-glucose 6-dehydrogenases Proteins 0.000 abstract 2
108010061048 UDPacetylglucosamine pyrophosphorylase Proteins 0.000 abstract 2
108010070600 Glucose-6-phosphate isomerase Proteins 0.000 abstract 1
108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
102100037921 UDP-N-acetylglucosamine pyrophosphorylase Human genes 0.000 abstract 1
102100029640 UDP-glucose 6-dehydrogenase Human genes 0.000 abstract 1
102000048175 UTP-glucose-1-phosphate uridylyltransferases Human genes 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
108090000623 proteins and genes Proteins 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Polysaccharides And Polysaccharide Derivatives (AREA)

AT02805661T 2001-12-21 2002-12-20 Verfahren zur produktion von hyaluronan in einer rekombinanten wirtszelle ATE502115T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US34264401P	2001-12-21	2001-12-21
PCT/US2002/041067 WO2003054163A2 (en)	2001-12-21	2002-12-20	Methods for producing hyaluronan in a recombinant host cell

Publications (1)

Publication Number	Publication Date
ATE502115T1 true ATE502115T1 (de)	2011-04-15

Family

ID=23342668

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT02805661T ATE502115T1 (de)	2001-12-21	2002-12-20	Verfahren zur produktion von hyaluronan in einer rekombinanten wirtszelle

Country Status (18)

Country	Link
US (5)	US7811806B2 (de)
EP (1)	EP1572895B1 (de)
JP (1)	JP2005525091A (de)
KR (2)	KR100885163B1 (de)
CN (1)	CN1636052B (de)
AT (1)	ATE502115T1 (de)
AU (1)	AU2002366711C1 (de)
BR (1)	BR0215220A (de)
CA (2)	CA2471148C (de)
CZ (1)	CZ2004765A3 (de)
DE (1)	DE60239495D1 (de)
DK (1)	DK1572895T3 (de)
ES (1)	ES2362354T3 (de)
HU (2)	HU228693B1 (de)
IL (2)	IL162302A0 (de)
PL (2)	PL212928B1 (de)
RU (1)	RU2346049C2 (de)
WO (1)	WO2003054163A2 (de)

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US7002007B2 (en) *	2004-05-28	2006-02-21	Calcigen Corporation	Production of high molecular weight hyaluronates
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EP1640457A1 (de) *	2004-09-23	2006-03-29	Bayer CropScience GmbH	Verfahren und Mittel zur Herstellung von Hyaluronan
US8481080B2 (en) *	2004-11-24	2013-07-09	Novozymes Biopolymer A/S	Method of cross-linking hyaluronic acid with divinulsulfone
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WO2008014787A1 (en)	2006-08-04	2008-02-07	Novozymes Biopolymer A/S	Branched hyaluronic acid and method of manufacture
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EP2031053A1 (de) *	2007-08-31	2009-03-04	The University Of Queensland	HA-Herstellung
EP2036983A1 (de) *	2007-09-12	2009-03-18	Bayer CropScience AG	Pflanzen, die erhöhte Mengen an Glucosaminglycanen synthetisieren
US9109051B2 (en) *	2007-12-19	2015-08-18	Evonik Goldschmidt Gmbh	Crosslinked hyaluronic acid in emulsion
EP2376626A4 (de)	2008-12-13	2012-10-17	Dna Microarray	MIKROUMGEBUNGSNISCHENTEST FÜR CiPS-SCREENING
EP2213315A1 (de)	2009-01-30	2010-08-04	Mero S.r.L.	Antibakterielles Hydrogel und dessen Verwendungen in der Orthopädie
CN102021213B (zh) *	2009-09-17	2014-07-09	上海佰加壹医药有限公司	一种发酵生产透明质酸发酵液的方法
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IT1401498B1 (it)	2010-07-30	2013-07-26	Mero Srl	Idrogelo a base di acido ialuronico e suo uso in ortopedia
IT1402385B1 (it)	2010-09-09	2013-09-04	Fidia Farmaceutici	Processo per la produzione di acido ialuronico in escherichia coli o bacillus subtilis
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JP2014515426A (ja)	2011-05-30	2014-06-30	ノボザイムスバイオファーマデーコーアクティーゼルスカブ	高分子量ヒアルロン酸の噴霧乾燥
CN102911887A (zh) *	2011-08-02	2013-02-06	山东省生物药物研究院	一种利用毕赤酵母基因调控发酵生产不同分子量透明质酸的方法
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CN106755022B (zh) *	2015-11-25	2020-08-04	中国科学院大连化学物理研究所	乙酰葡萄糖胺磷酸变位酶AtAGM编码基因及其酶、制备、应用与酶活性检测方法
US10662417B2 (en)	2016-07-05	2020-05-26	Novozymes A/S	Pectate lyase variants and polynucleotides encoding same
CN107653280B (zh) *	2016-07-25	2021-05-04	安琪酵母股份有限公司	透明质酸发酵用的组合物、培养基和方法及其应用
CN106381279B (zh) *	2016-08-29	2019-11-05	中国药科大学	一种细菌胞外多糖、其制备方法及其应用
KR102135044B1 (ko) *	2018-12-10	2020-07-17	대화제약 주식회사	히알루론산 합성효소 변이 단백질 및 이를 이용한 히알루론산 생산 방법
WO2020122430A1 (ko) *	2018-12-10	2020-06-18	대화제약 주식회사	비병원성 세균을 이용하여 히알루론산 생산을 위한 발현 시스템 및 상기 발현 시스템을 이용한 히알루론산 생산방법
WO2020122429A1 (ko) *	2018-12-10	2020-06-18	대화제약 주식회사	히알루론산 합성효소 변이 단백질 및 이를 이용한 히알루론산 생산 방법
CN110055201B (zh) *	2019-03-05	2021-05-28	江南大学	一种高产透明质酸寡糖的重组枯草芽孢杆菌的构建方法
RU2719140C1 (ru)	2019-07-26	2020-04-17	Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех"	Бактерия рода bacillus, продуцирующая гиалуроновую кислоту, и способ получения гиалуроновой кислоты с использованием указанной бактерии
RU2723722C1 (ru) *	2019-07-26	2020-06-17	Общество с ограниченной ответственностью "ЦЕНТР ТРАНСФЕРА БИОТЕХНОЛОГИЙ ОКА-Биотех"	ТАНДЕМНЫЙ ПРОМОТОР, ФУНКЦИОНИРУЮЩИЙ В БАКТЕРИИ РОДА Bacillus, ТРАНСФОРМИРОВАННАЯ БАКТЕРИЯ РОДА Bacillus - ПРОДУЦЕНТ ЦЕЛЕВОГО ПРОДУКТА, СОДЕРЖАЩАЯ УКАЗАННЫЙ ПРОМОТОР, И СПОСОБ ПОЛУЧЕНИЯ ЦЕЛЕВОГО ПРОДУКТА С ИСПОЛЬЗОВАНИЕМ УКАЗАННОЙ БАКТЕРИИ
CN111518825B (zh) *	2020-04-30	2022-10-11	浙江工业大学	一种多基因组合表达制备蛹虫草多糖的方法
WO2022178432A1 (en) *	2021-02-22	2022-08-25	Danisco Us Inc.	Methods and compositions for producing proteins of interest in pigment deficient bacillus cells
BR112023019830A2 (pt) *	2021-04-01	2024-01-16	Givaudan Sa	Ácido hialurônico, célula de levedura e célula hospedeira recombinantes que produzem o mesmo, meio de cultivo, composição, produto industrial ou produto de consumo ou consumível, uso de uma célula recombinante e método de produção do mesmo
EP4487863A1 (de)	2023-07-07	2025-01-08	NuPep AS	Konsensuspeptide in hya zur verwendung bei der behandlung degenerierter gelenke
WO2025012149A1 (en)	2023-07-07	2025-01-16	Nupep As	Consensus peptides in hya
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KR20250148458A (ko) *	2024-04-01	2025-10-14	씨제이제일제당 (주)	신규한 바실러스 프로모터 변이체 및 이를 이용한 단백질 생산 방법

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2002
- 2002-12-20 AU AU2002366711A patent/AU2002366711C1/en not_active Expired
- 2002-12-20 AT AT02805661T patent/ATE502115T1/de active
- 2002-12-20 CA CA2471148A patent/CA2471148C/en not_active Expired - Lifetime
- 2002-12-20 WO PCT/US2002/041067 patent/WO2003054163A2/en not_active Ceased
- 2002-12-20 IL IL16230202A patent/IL162302A0/xx active IP Right Grant
- 2002-12-20 CZ CZ2004765A patent/CZ2004765A3/cs unknown
- 2002-12-20 EP EP02805661A patent/EP1572895B1/de not_active Expired - Lifetime
- 2002-12-20 DE DE60239495T patent/DE60239495D1/de not_active Expired - Lifetime
- 2002-12-20 RU RU2004122420/13A patent/RU2346049C2/ru active
- 2002-12-20 PL PL375190A patent/PL212928B1/pl unknown
- 2002-12-20 BR BRPI0215220-7A patent/BR0215220A/pt not_active Application Discontinuation
- 2002-12-20 JP JP2003554868A patent/JP2005525091A/ja active Pending
- 2002-12-20 HU HU0700154A patent/HU228693B1/hu unknown
- 2002-12-20 CN CN02828283.3A patent/CN1636052B/zh not_active Expired - Lifetime
- 2002-12-20 KR KR1020087002156A patent/KR100885163B1/ko not_active Expired - Lifetime
- 2002-12-20 KR KR1020047009883A patent/KR100879908B1/ko not_active Expired - Lifetime
- 2002-12-20 ES ES02805661T patent/ES2362354T3/es not_active Expired - Lifetime
- 2002-12-20 CA CA2803931A patent/CA2803931A1/en not_active Abandoned
- 2002-12-20 PL PL399352A patent/PL399352A1/pl unknown
- 2002-12-20 DK DK02805661.2T patent/DK1572895T3/da active
- 2002-12-20 US US10/326,185 patent/US7811806B2/en not_active Expired - Lifetime
2004
- 2004-06-02 IL IL162302A patent/IL162302A/en unknown
2007
- 2007-05-29 HU HUP1300136 patent/HU1300136D0/hu not_active Application Discontinuation
2010
- 2010-09-27 US US12/891,548 patent/US8093036B2/en not_active Expired - Fee Related
2011
- 2011-04-11 US US13/084,230 patent/US8137951B2/en not_active Expired - Fee Related
2012
- 2012-02-21 US US13/401,663 patent/US8574886B2/en not_active Expired - Fee Related
2013
- 2013-10-10 US US14/050,866 patent/US20140038235A1/en not_active Abandoned

Also Published As

Publication number	Publication date
CA2803931A1 (en)	2003-07-03
US20110189737A1 (en)	2011-08-04
EP1572895A4 (de)	2007-07-25
US7811806B2 (en)	2010-10-12
KR100885163B1 (ko)	2009-02-23
RU2004122420A (ru)	2005-10-10
PL375190A1 (en)	2005-11-28
IL162302A0 (en)	2005-11-20
CZ2004765A3 (cs)	2004-12-15
PL399352A1 (pl)	2012-10-08
US8093036B2 (en)	2012-01-10
EP1572895A2 (de)	2005-09-14
US20140038235A1 (en)	2014-02-06
US8137951B2 (en)	2012-03-20
ES2362354T3 (es)	2011-07-04
HU228693B1 (hu)	2013-05-28
IL162302A (en)	2011-01-31
PL212928B1 (pl)	2012-12-31
HU1300136D0 (hu)	2007-05-29
CN1636052B (zh)	2014-07-02
US20120149067A1 (en)	2012-06-14
JP2005525091A (ja)	2005-08-25
RU2346049C2 (ru)	2009-02-10
DK1572895T3 (da)	2011-07-11
WO2003054163A3 (en)	2005-10-27
EP1572895B1 (de)	2011-03-16
AU2002366711A1 (en)	2003-07-09
US8574886B2 (en)	2013-11-05
HUP0700154A2 (en)	2007-05-29
CA2471148C (en)	2013-04-09
DE60239495D1 (de)	2011-04-28
AU2002366711C1 (en)	2009-01-22
BR0215220A (pt)	2007-01-09
KR20040085142A (ko)	2004-10-07
CA2471148A1 (en)	2003-07-03
US20030175902A1 (en)	2003-09-18
AU2002366711B2 (en)	2007-11-29
WO2003054163A2 (en)	2003-07-03
KR100879908B1 (ko)	2009-01-21
US20110014662A1 (en)	2011-01-20
CN1636052A (zh)	2005-07-06
KR20080018965A (ko)	2008-02-28

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