ATE504661T1 - Methode für die analyse von methylierter dna - Google Patents
Methode für die analyse von methylierter dnaInfo
- Publication number
- ATE504661T1 ATE504661T1 AT04090321T AT04090321T ATE504661T1 AT E504661 T1 ATE504661 T1 AT E504661T1 AT 04090321 T AT04090321 T AT 04090321T AT 04090321 T AT04090321 T AT 04090321T AT E504661 T1 ATE504661 T1 AT E504661T1
- Authority
- AT
- Austria
- Prior art keywords
- oligonucleotide
- dna
- extendable
- extended
- methylation status
- Prior art date
Links
- 108091034117 Oligonucleotide Proteins 0.000 abstract 5
- 230000011987 methylation Effects 0.000 abstract 3
- 238000007069 methylation reaction Methods 0.000 abstract 3
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical class CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 abstract 1
- 230000002411 adverse Effects 0.000 abstract 1
- 230000024245 cell differentiation Effects 0.000 abstract 1
- 238000003745 diagnosis Methods 0.000 abstract 1
- 238000004393 prognosis Methods 0.000 abstract 1
- 229940035893 uracil Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP04090321A EP1627924B1 (de) | 2004-08-19 | 2004-08-19 | Methode für die Analyse von methylierter DNA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE504661T1 true ATE504661T1 (de) | 2011-04-15 |
Family
ID=34928817
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04090321T ATE504661T1 (de) | 2004-08-19 | 2004-08-19 | Methode für die analyse von methylierter dna |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20070254293A1 (de) |
| EP (1) | EP1627924B1 (de) |
| AT (1) | ATE504661T1 (de) |
| DE (1) | DE602004032126D1 (de) |
| WO (1) | WO2006018243A1 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2189538A1 (de) * | 2008-11-21 | 2010-05-26 | Epigenomics AG | Analysemolekül und Verfahren zur Analyse einer Nucleotid-Position in einer Nukleinsäure |
| CN109517888B (zh) * | 2013-04-01 | 2022-07-26 | 基因特力株式会社 | 使用等位基因特异的反应性引物的核酸扩增方法 |
| IL280222B2 (en) | 2018-07-19 | 2025-08-01 | Biofidelity Ltd | Improved polynucleotide sequence detection method |
| GB201919235D0 (en) * | 2019-12-23 | 2020-02-05 | Biofidelity Ltd | Methylation detection method |
| GB201919186D0 (en) | 2019-12-23 | 2020-02-05 | Biofidelity Ltd | Simplified polynucleotide sequence detection method |
| WO2025124705A1 (en) * | 2023-12-13 | 2025-06-19 | Universität Konstanz | Dna polymerases for the detection of epigenetic dna marks |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5786146A (en) * | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| US7033763B2 (en) * | 2000-02-23 | 2006-04-25 | City Of Hope | Pyrophosphorolysis activated polymerization (PAP) |
| AUPR050700A0 (en) * | 2000-10-03 | 2000-10-26 | Id+Plus Ltd | Detection method |
| DE10112515B4 (de) * | 2001-03-09 | 2004-02-12 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität |
-
2004
- 2004-08-19 AT AT04090321T patent/ATE504661T1/de not_active IP Right Cessation
- 2004-08-19 EP EP04090321A patent/EP1627924B1/de not_active Expired - Lifetime
- 2004-08-19 DE DE602004032126T patent/DE602004032126D1/de not_active Expired - Lifetime
-
2005
- 2005-08-09 US US11/660,804 patent/US20070254293A1/en not_active Abandoned
- 2005-08-09 WO PCT/EP2005/008792 patent/WO2006018243A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP1627924A1 (de) | 2006-02-22 |
| DE602004032126D1 (de) | 2011-05-19 |
| WO2006018243A1 (en) | 2006-02-23 |
| US20070254293A1 (en) | 2007-11-01 |
| EP1627924B1 (de) | 2011-04-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |