ATE518002T1 - Enzymatischer zyklierungsassay für homocystein und cystathionin - Google Patents
Enzymatischer zyklierungsassay für homocystein und cystathioninInfo
- Publication number
- ATE518002T1 ATE518002T1 AT00976976T AT00976976T ATE518002T1 AT E518002 T1 ATE518002 T1 AT E518002T1 AT 00976976 T AT00976976 T AT 00976976T AT 00976976 T AT00976976 T AT 00976976T AT E518002 T1 ATE518002 T1 AT E518002T1
- Authority
- AT
- Austria
- Prior art keywords
- cystathionine
- homocysteine
- amount
- derivatives
- assay
- Prior art date
Links
- FFFHZYDWPBMWHY-VKHMYHEASA-N L-homocysteine Chemical compound OC(=O)[C@@H](N)CCS FFFHZYDWPBMWHY-VKHMYHEASA-N 0.000 title abstract 7
- YPWSLBHSMIKTPR-UHFFFAOYSA-N Cystathionine Natural products OC(=O)C(N)CCSSCC(N)C(O)=O YPWSLBHSMIKTPR-UHFFFAOYSA-N 0.000 title abstract 6
- ILRYLPWNYFXEMH-UHFFFAOYSA-N D-cystathionine Natural products OC(=O)C(N)CCSCC(N)C(O)=O ILRYLPWNYFXEMH-UHFFFAOYSA-N 0.000 title abstract 6
- ILRYLPWNYFXEMH-WHFBIAKZSA-N L-cystathionine Chemical compound [O-]C(=O)[C@@H]([NH3+])CCSC[C@H]([NH3+])C([O-])=O ILRYLPWNYFXEMH-WHFBIAKZSA-N 0.000 title abstract 6
- 238000003556 assay Methods 0.000 title abstract 5
- 230000001351 cycling effect Effects 0.000 title abstract 2
- 230000002255 enzymatic effect Effects 0.000 title abstract 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract 6
- 229910021529 ammonia Inorganic materials 0.000 abstract 3
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 abstract 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 abstract 2
- 239000008280 blood Substances 0.000 abstract 2
- 210000004369 blood Anatomy 0.000 abstract 2
- 239000011541 reaction mixture Substances 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 238000003149 assay kit Methods 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 229960001153 serine Drugs 0.000 abstract 1
- 210000002700 urine Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US16312699P | 1999-11-02 | 1999-11-02 | |
| US20334900P | 2000-05-10 | 2000-05-10 | |
| US09/704,036 US6664073B1 (en) | 1999-11-02 | 2000-11-01 | Enzymatic cycling assays for homocysteine and cystathionine |
| PCT/US2000/030518 WO2001033187A2 (en) | 1999-11-02 | 2000-11-02 | Enzymatic cycling assays for homocysteine and cystathionine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE518002T1 true ATE518002T1 (de) | 2011-08-15 |
Family
ID=27388839
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00976976T ATE518002T1 (de) | 1999-11-02 | 2000-11-02 | Enzymatischer zyklierungsassay für homocystein und cystathionin |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6664073B1 (de) |
| EP (1) | EP1230381B1 (de) |
| AT (1) | ATE518002T1 (de) |
| AU (1) | AU1467601A (de) |
| DK (1) | DK1230381T3 (de) |
| WO (1) | WO2001033187A2 (de) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6664073B1 (en) | 1999-11-02 | 2003-12-16 | Catch Inc. | Enzymatic cycling assays for homocysteine and cystathionine |
| US6635438B2 (en) * | 1999-11-02 | 2003-10-21 | Catch, Inc. | Enzymatic cycling assays for homocysteine and cystathionine |
| US7097968B2 (en) * | 2003-07-10 | 2006-08-29 | General Atomics | Methods and compositions for assaying homocysteine |
| US8476034B2 (en) * | 2003-07-10 | 2013-07-02 | General Atomics | Methods and compositions for assaying homocysteine |
| US9201075B2 (en) * | 2004-04-30 | 2015-12-01 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Colorimetric and fluorometric determination of homocysteine and cysteine |
| WO2014026157A2 (en) * | 2012-08-10 | 2014-02-13 | Cedars-Sinai Medical Center | Methionine metabolites predict aggressive cancer progression |
| CN106248666A (zh) * | 2016-10-06 | 2016-12-21 | 济南天舜生物技术有限公司 | 一种稳定性强的同型半胱氨酸（hcy）检测试剂 |
| WO2018174858A1 (en) * | 2017-03-21 | 2018-09-27 | Mprobe Inc. | Clinical method for the population screening of adult metabolic disorder associated with chronic human disease |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6066467A (en) * | 1997-07-24 | 2000-05-23 | Anticancer, Inc. | High specificity homocysteine assays for biological samples |
| CA2350607C (en) * | 1998-11-12 | 2009-04-07 | Axis - Shield Diagnostics, Ltd. | A method for the determination of homocysteine |
| US6174696B1 (en) * | 1999-11-12 | 2001-01-16 | Genzyme Corporation | Method for the determination of homocysteine |
| US6664073B1 (en) | 1999-11-02 | 2003-12-16 | Catch Inc. | Enzymatic cycling assays for homocysteine and cystathionine |
-
2000
- 2000-11-01 US US09/704,036 patent/US6664073B1/en not_active Expired - Lifetime
- 2000-11-02 EP EP00976976A patent/EP1230381B1/de not_active Expired - Lifetime
- 2000-11-02 AU AU14676/01A patent/AU1467601A/en not_active Abandoned
- 2000-11-02 WO PCT/US2000/030518 patent/WO2001033187A2/en not_active Ceased
- 2000-11-02 DK DK00976976.1T patent/DK1230381T3/da active
- 2000-11-02 AT AT00976976T patent/ATE518002T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| US6664073B1 (en) | 2003-12-16 |
| WO2001033187A3 (en) | 2001-09-27 |
| EP1230381A2 (de) | 2002-08-14 |
| DK1230381T3 (da) | 2011-10-31 |
| EP1230381A4 (de) | 2004-10-13 |
| AU1467601A (en) | 2001-05-14 |
| WO2001033187A2 (en) | 2001-05-10 |
| EP1230381B1 (de) | 2011-07-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2003040694A3 (en) | Enzymatic cycling assays for homocysteine and cystathionine | |
| Marsh et al. | Coupling of cobalt− carbon bond homolysis and hydrogen atom abstraction in adenosylcobalamin-dependent glutamate mutase | |
| CN101900735B (zh) | 用于测定高半胱氨酸的方法 | |
| Frey | Travels with carbon-centered radicals. 5′-deoxyadenosine and 5′-deoxyadenosine-5′-yl in radical enzymology | |
| Faraci et al. | Mechanism of inactivation of alanine racemase by. beta.,. beta.,. beta.-trifluoroalanine | |
| Fujiwara et al. | Mechanism of the glycine cleavage reaction. Further characterization of the intermediate attached to H-protein and of the reaction catalyzed by T-protein. | |
| DE69837537D1 (de) | Methoden zur messung zellulärer vermehrungs- und abbauraten in vitro und in vivo | |
| ES2183482T3 (es) | Dispositivo de diagnostico a base de compuestos de tetrazolio. | |
| JPS56124395A (en) | Stabilized coenzyme aqueous solution | |
| DK1230381T3 (da) | Enzymatiske cyklusassays for homocystein og cystathionin | |
| SE7407447L (de) | ||
| JPH04278099A (ja) | アンモニア、α−アミノ酸類またはα−ケト酸の高感度定量法および高感度定量用組成物 | |
| Joh et al. | A simple and sensitive assay for dopamine-β-hydroxylase | |
| EP0121254A3 (en) | Process for determining substrate or enzymatic activity | |
| ES2110979T3 (es) | Analisis enzimaticos. | |
| BR9914952A (pt) | Processo e kit de teste para análise de reparo de dna | |
| London et al. | A systematic approach to enzyme assay optimization, illustrated by aminotransferase assays | |
| DE60037311D1 (de) | Homogene enzymatische bestimmungsmethode für vitamin b6 | |
| US3994783A (en) | Differential assay of creatine phosphokinase isoenzymes | |
| NO980812L (no) | Anvendelse av vanadiumbromperoksydase som et signalgenererende enzym for kjemilumineserende systemer, testkits og analytiske metoder | |
| CH647600A5 (it) | Kit diagnostico e uso del kit per la determinazione delle transaminasi. | |
| IL96115A0 (en) | Enzymatic assay and assay kits for detecting and/or measuring the activation of cells | |
| Kiick | Effect of commitments to catalysis on the degree of curvature in proton inventories of the kinetic parameters for enzyme-catalyzed reactions: application to tryptophan indole-lyase | |
| ES8500991A1 (es) | Un acido l-glutamico oxidasa. | |
| Matherly et al. | Transient-state kinetic analysis of urocanase |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |