ES2110979T3 - Analisis enzimaticos. - Google Patents

Analisis enzimaticos.

Info

Publication number
ES2110979T3
ES2110979T3 ES91308338T ES91308338T ES2110979T3 ES 2110979 T3 ES2110979 T3 ES 2110979T3 ES 91308338 T ES91308338 T ES 91308338T ES 91308338 T ES91308338 T ES 91308338T ES 2110979 T3 ES2110979 T3 ES 2110979T3
Authority
ES
Spain
Prior art keywords
enzymatic analysis
techniques
assays
enzymes
dimerisation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91308338T
Other languages
English (en)
Inventor
Akio Tsuji
Masako Maeda
Hidetoshi Arakawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sankyo Co Ltd
Original Assignee
Sankyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sankyo Co Ltd filed Critical Sankyo Co Ltd
Application granted granted Critical
Publication of ES2110979T3 publication Critical patent/ES2110979T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B7/00—Indigoid dyes
    • C09B7/02—Bis-indole indigos
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/50—Indoles
    • C12Q2334/52—5-Bromo-4-chloro-3-indolyl, i.e. BCI

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

ENSAYOS PRECISOS DE ENZIMAS ADECUADOS PARA UTILIZAR EN TECNICAS DE INMUNOENSAYO ACTUALMENTE SON POSIBLES PARA LAS ENZIMAS ASOCIADAS CON LA DIMERIZACION DE ESTERES INDOXIL PARA FORMAR EL CORRESPONDIENTE INDIGO, UTILIZANDO LOS ENSAYOS LA PRODUCCION DE HIDROGENO PEROXIDO DE ESTA REACCION, QUE SE ENCUENTRA SUFICIENTEMENTE CONSISTENTE QUE PUEDE SER UTILIZADA PARA CALCULAR CON PRECISION LA ACTIVIDAD DE ENZIMAS A NIVELES DE 1 A 1000 COMPARADO CON LAS TECNICAS COLORIMETRICAS PARA LAS MISMAS ENZIMAS.
ES91308338T 1990-09-12 1991-09-12 Analisis enzimaticos. Expired - Lifetime ES2110979T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP24001890 1990-09-12

Publications (1)

Publication Number Publication Date
ES2110979T3 true ES2110979T3 (es) 1998-03-01

Family

ID=17053242

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91308338T Expired - Lifetime ES2110979T3 (es) 1990-09-12 1991-09-12 Analisis enzimaticos.

Country Status (9)

Country Link
EP (1) EP0476930B1 (es)
JP (1) JPH04356200A (es)
AT (1) ATE160177T1 (es)
CA (1) CA2051144A1 (es)
DE (1) DE69128181T2 (es)
DK (1) DK0476930T3 (es)
ES (1) ES2110979T3 (es)
GR (1) GR3025741T3 (es)
HK (1) HK1003840A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5589328A (en) * 1994-08-04 1996-12-31 Mahant; Vijay K. Chemiluminescence assays based on indoxyl substrates, thioindoxyl substrates and other substrates
ES2143934B1 (es) * 1998-01-23 2000-12-16 Univ Oviedo Cuantificacion amperometrica y voltamperometrica de azul de indigo para la determinacion de hidrolasas y desarrollo de biosensores.
US7390670B2 (en) 2003-02-20 2008-06-24 Lumigen, Inc. Signalling compounds and methods for detecting hydrogen peroxide
JP4627972B2 (ja) * 2003-06-12 2011-02-09 新潟県 清酒酵母識別培地及び清酒酵母以外の酵母の存在確認方法
JP2006025608A (ja) * 2004-07-12 2006-02-02 Chisso Corp 微生物培地
CN104101595A (zh) * 2013-04-08 2014-10-15 张岩 一种法呢基焦磷酸合酶等类酶的活性测定方法
CN103837529B (zh) * 2014-03-06 2016-08-24 北京工业大学 一种用于检测水中含氢量的检测管及应用
CN104374768B (zh) * 2014-09-03 2017-05-17 西南大学 一种快速定量检测氨基酸脱羧酶的比色方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1128371A (en) * 1965-10-04 1968-09-25 Miles Lab Diagnostic composition
EP0243126B1 (en) * 1986-04-19 1992-08-12 Sanwa Kagaku Kenkyusho Co., Ltd. Method of measuring hydrogen peroxide or of measuring an enzyme or biochemical substrate liberating the peroxide
US4999284A (en) * 1988-04-06 1991-03-12 E. I. Du Pont De Nemours And Company Enzymatically amplified piezoelectric specific binding assay
US4942127A (en) * 1988-05-06 1990-07-17 Molecular Devices Corporation Polyredox couples in analyte determinations

Also Published As

Publication number Publication date
DK0476930T3 (da) 1998-07-27
EP0476930B1 (en) 1997-11-12
JPH04356200A (ja) 1992-12-09
DE69128181T2 (de) 1998-05-28
HK1003840A1 (en) 1998-11-06
CA2051144A1 (en) 1992-03-13
DE69128181D1 (de) 1997-12-18
EP0476930A1 (en) 1992-03-25
GR3025741T3 (en) 1998-03-31
ATE160177T1 (de) 1997-11-15

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