ATE518010T1 - Aktive oberflächengekoppelte polymerasen - Google Patents

Aktive oberflächengekoppelte polymerasen

Info

Publication number
ATE518010T1
ATE518010T1 AT06848008T AT06848008T ATE518010T1 AT E518010 T1 ATE518010 T1 AT E518010T1 AT 06848008 T AT06848008 T AT 06848008T AT 06848008 T AT06848008 T AT 06848008T AT E518010 T1 ATE518010 T1 AT E518010T1
Authority
AT
Austria
Prior art keywords
active surface
surface coupled
polymerases
coupled polymerases
making
Prior art date
Application number
AT06848008T
Other languages
English (en)
Inventor
David Hanzel
Jonas Korlach
Paul Peluso
Geoffrey Otto
Thang Pham
David Rank
Stephen Turner
Original Assignee
Pacific Biosciences California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pacific Biosciences California filed Critical Pacific Biosciences California
Application granted granted Critical
Publication of ATE518010T1 publication Critical patent/ATE518010T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT06848008T 2005-12-22 2006-12-21 Aktive oberflächengekoppelte polymerasen ATE518010T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US75351505P 2005-12-22 2005-12-22
PCT/US2006/048973 WO2007075987A2 (en) 2005-12-22 2006-12-21 Active surface coupled polymerases

Publications (1)

Publication Number Publication Date
ATE518010T1 true ATE518010T1 (de) 2011-08-15

Family

ID=38218654

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06848008T ATE518010T1 (de) 2005-12-22 2006-12-21 Aktive oberflächengekoppelte polymerasen

Country Status (6)

Country Link
US (2) US8936926B2 (de)
EP (1) EP1963530B1 (de)
AT (1) ATE518010T1 (de)
AU (1) AU2006331512B2 (de)
CA (1) CA2633476C (de)
WO (1) WO2007075987A2 (de)

Families Citing this family (159)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
CA2633524A1 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
US8889348B2 (en) 2006-06-07 2014-11-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by nanopore using modified nucleotides
US8003330B2 (en) 2007-09-28 2011-08-23 Pacific Biosciences Of California, Inc. Error-free amplification of DNA for clonal sequencing
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
US7767441B2 (en) 2007-10-25 2010-08-03 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
US7811810B2 (en) 2007-10-25 2010-10-12 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
WO2009114182A1 (en) 2008-03-13 2009-09-17 Pacific Biosciences Of California, Inc. Labeled reactants and their uses
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
EP3269824A1 (de) 2008-03-28 2018-01-17 Pacific Biosciences Of California, Inc. Zusammensetzungen und verfahren zur nukleinsäuresequenzierung
US8420366B2 (en) 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8906831B2 (en) 2008-03-31 2014-12-09 Pacific Biosciences Of California, Inc. Single molecule loading methods and compositions
US9127259B2 (en) 2008-03-31 2015-09-08 Pacific Biosciences Of California, Inc. Enzymes resistant to photodamage
US8257954B2 (en) 2008-03-31 2012-09-04 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
EP2274446B1 (de) * 2008-03-31 2015-09-09 Pacific Biosciences of California, Inc. Systeme und verfahren für polymeraseenzymreaktionen in zwei langsamen schritten
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
EP2307540B1 (de) 2008-07-07 2017-04-19 Oxford Nanopore Technologies Limited Enzymporenstrukturen
WO2010004273A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
CA2735979A1 (en) 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc. Engineering polymerases and reaction conditions for modified incorporation properties
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
US20120100530A1 (en) 2009-01-30 2012-04-26 Oxford Nanopore Technologies Limited Enzyme mutant
WO2010086622A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
US9778188B2 (en) 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
WO2011031313A2 (en) 2009-09-11 2011-03-17 Pacific Biosciences Of California, Inc. Zero-mode waveguides with non-reflecting walls
US8367159B2 (en) 2009-09-11 2013-02-05 Pacific Biosciences Of California, Inc. Methods for producing ZMWs with islands of functionality
US8911972B2 (en) 2009-12-16 2014-12-16 Pacific Biosciences Of California, Inc. Sequencing methods using enzyme conformation
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US20110192723A1 (en) * 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
EP3460458B1 (de) 2010-02-19 2021-08-11 Pacific Biosciences of California, Inc. Verfahren zur nukleinsäuresequenzierung
US9482615B2 (en) 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
WO2011139344A2 (en) 2010-04-28 2011-11-10 Pacific Biosciences Of California, Inc Nanoscale apertures having islands of functionality
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8865077B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
WO2012009206A2 (en) 2010-07-12 2012-01-19 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
US8889373B2 (en) 2010-08-12 2014-11-18 Eastman Chemical Company Enzyme catalyst immobilized on porous fluoropolymer support
US8889886B2 (en) 2010-08-25 2014-11-18 Pacific Biosciences, Inc. Cyanine dyes
US8936911B2 (en) 2010-09-22 2015-01-20 Pacific Biosciences Of California, Inc. Purified extended polymerase/template complex for sequencing
EP2652153B1 (de) 2010-12-17 2017-07-05 The Trustees of Columbia University in the City of New York Dna-sequenzierung durch synthese unter verwendung modifizierter nukleotide und nanoporennachweis
WO2012088339A2 (en) 2010-12-22 2012-06-28 Genia Technologies, Inc. Nanopore-based single dna molecule characterization using speed bumps
SG191409A1 (en) 2010-12-27 2013-08-30 Ibis Biosciences Inc Nucleic acid sample preparation methods and compositions
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
EP2673638B1 (de) 2011-02-11 2019-10-30 Oxford Nanopore Technologies Limited Mutante poren
IN2014DN00221A (de) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
WO2013022778A1 (en) 2011-08-05 2013-02-14 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
WO2013056241A2 (en) 2011-10-14 2013-04-18 Pacific Biosciences Of California, Inc. Real-time redox sequencing
WO2013101741A1 (en) 2011-12-30 2013-07-04 Abbott Molecular, Inc. Channels with cross-sectional thermal gradients
CN108611398A (zh) 2012-01-13 2018-10-02 Data生物有限公司 通过新一代测序进行基因分型
US8906660B2 (en) 2012-02-01 2014-12-09 Pacific Biosciences Of California, Inc. Recombinant polymerases with increased phototolerance
EP3590948A1 (de) 2012-02-15 2020-01-08 Pacific Biosciences of California, Inc. Polymeraseenzymsubstrate mit proteinabschirmung
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
US10246479B2 (en) 2012-04-09 2019-04-02 The Trustees Of Columbia University In The City Of New York Method of preparation of nanopore and uses thereof
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
ES2683707T3 (es) 2012-05-02 2018-09-27 Ibis Biosciences, Inc. Secuenciación de ADN
EP3438286B1 (de) 2012-05-02 2020-10-28 Ibis Biosciences, Inc. Dna-sequenzierung
ES2833524T3 (es) 2012-05-02 2021-06-15 Ibis Biosciences Inc Secuenciación de ADN
EP2850086B1 (de) 2012-05-18 2023-07-05 Pacific Biosciences Of California, Inc. Heteroarylcyaninfarbstoffe
US9315864B2 (en) 2012-05-18 2016-04-19 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes with sulfonic acid substituents
US9116118B2 (en) 2012-06-08 2015-08-25 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
GB2510719A (en) 2012-06-15 2014-08-13 Genia Technologies Inc Chip set-up and high-accuracy nucleic acid sequencing
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
ES2701750T3 (es) 2012-10-16 2019-02-25 Abbott Molecular Inc Procedimientos para secuenciar un ácido nucleico
WO2014071070A1 (en) 2012-11-01 2014-05-08 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
BR112015021788B1 (pt) 2013-03-08 2023-02-28 Oxford Nanopore Technologies Plc Métodos para mover uma ou mais helicases imobilizadas, para controlar o movimento de um polinucleotídeo alvo, para caracterizar um polinucleotídeo alvo e para controlar o carregamento de uma ou mais helicases em um polinucleotídeo alvo, uso de um poro de transmembrana e de um potencial aplicado e de um ou mais espaçadores, complexo, e, kit
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
CN105102627B (zh) 2013-03-15 2018-10-19 纽约哥伦比亚大学理事会 用于检测样品中多种预定化合物的方法
US9290800B2 (en) 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
EP3575414B1 (de) 2013-05-06 2023-09-06 Pacific Biosciences of California, Inc. Elektronische echtzeitsequenzierung
US10544449B2 (en) 2013-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
US9678080B2 (en) 2013-06-14 2017-06-13 Pacific Biosciences Of California, Inc. Bis-biotinylation tags
US20150051088A1 (en) 2013-08-19 2015-02-19 Abbott Molecular Inc. Next-generation sequencing libraries
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US9322062B2 (en) 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
EP3640349A3 (de) 2013-10-23 2020-07-29 Roche Sequencing Solutions, Inc. Schnelle molekulare erfassung mit nanoporen
JP6573899B2 (ja) 2013-11-17 2019-09-11 クアンタム−エスアイ インコーポレイテッドＱｕａｎｔｕｍ−Ｓｉ Ｉｎｃｏｒｐｏｒａｔｅｄ 分子をプローブし、検出し、及び分析するための、外部光源を備えた集積デバイス
EP3083700B1 (de) 2013-12-17 2023-10-11 The Brigham and Women's Hospital, Inc. Bestimmung eines antikörpers gegen ein pathogen
CA2937411C (en) 2014-01-22 2023-09-26 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
CA2943952A1 (en) 2014-03-24 2015-10-01 The Trustees Of Columbia University In The City Of New York Chemical methods for producing tagged nucleotides
EP3137627B1 (de) 2014-05-02 2025-07-30 Oxford Nanopore Technologies plc Verfahren zur verbesserung der bewegung eines zielpolynukleotids bezüglich einer transmembranpore
WO2015200541A1 (en) 2014-06-24 2015-12-30 Bio-Rad Laboratories, Inc. Digital pcr barcoding
WO2015200833A2 (en) 2014-06-27 2015-12-30 Abbott Laboratories COMPOSITIONS AND METHODS FOR DETECTING HUMAN PEGIVIRUS 2 (HPgV-2)
EP3194935B1 (de) 2014-08-08 2018-10-31 Quantum-si Incorporated Integrierte vorrichtung zur temporären gruppierung von empfangenen photonen
WO2016023010A1 (en) 2014-08-08 2016-02-11 Quantum-Si Incorporated Optical system and assay chip for probing, detecting, and analyzing molecules
CN112903639B (zh) 2014-08-08 2022-09-13 宽腾矽公司 用于对分子进行探测、检测和分析的带外部光源的集成装置
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
US10435685B2 (en) 2014-08-19 2019-10-08 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
WO2016028887A1 (en) 2014-08-19 2016-02-25 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
KR20170042794A (ko) 2014-09-01 2017-04-19 브이아이비 브이지더블유 돌연변이체 csgg 포어
WO2016044391A1 (en) 2014-09-17 2016-03-24 Ibis Biosciences, Inc. Sequencing by synthesis using pulse read optics
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
EP3274473B1 (de) 2015-03-24 2020-10-28 Pacific Biosciences of California, Inc. Verfahren und zusammensetzungen zum laden einer einzelmolekülzusammensetzung
WO2016166232A1 (en) 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
WO2016174271A1 (en) 2015-04-30 2016-11-03 Curevac Ag Immobilized poly(n)polymerase
EP3332032B1 (de) 2015-08-06 2023-12-27 Pacific Biosciences Of California, Inc. Integrierte schaltungen zur selektiven adressierung spärlich angeordneter, elektronischer messvorrichtungen
WO2017027518A1 (en) 2015-08-10 2017-02-16 Stratos Genomics, Inc. Single molecule nucleic acid sequencing with molecular sensor complexes
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
US10731211B2 (en) 2015-11-18 2020-08-04 Pacific Biosciences Of California, Inc. Methods and compositions for loading of polymerase complexes
CN108350491A (zh) 2015-11-18 2018-07-31 加利福尼亚太平洋生物科学股份有限公司 将核酸加载到基材上
US10781483B2 (en) 2015-11-20 2020-09-22 Pacific Biosciences Of California, Inc. Labeled nucleotide analogs, reaction mixtures, and methods and systems for sequencing
WO2017087974A1 (en) 2015-11-20 2017-05-26 Pacific Biosciences Of California, Inc. Protected dye-labeled reagents
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
EP3400298B1 (de) 2016-01-08 2024-03-06 Bio-Rad Laboratories, Inc. Auflösung mehrerer beads pro tropfen
TW202139919A (zh) 2016-02-17 2021-11-01 美商太斯萊特健康股份有限公司 用於生命期成像及偵測應用之感測器及裝置
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
EP4122949B1 (de) 2016-04-06 2024-06-05 Oxford Nanopore Technologies plc Mutante pore
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN106021749B (zh) * 2016-05-25 2019-03-05 上海交通大学 反推二维格栅的稠度优化实现方法
US10544457B2 (en) 2016-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Methods and compositions for enriching compositions for polymerase enzyme complexes
US10711300B2 (en) 2016-07-22 2020-07-14 Pacific Biosciences Of California, Inc. Methods and compositions for delivery of molecules and complexes to reaction sites
WO2018042251A1 (en) 2016-08-29 2018-03-08 Oslo Universitetssykehus Hf Chip-seq assays
JP6941680B2 (ja) 2016-10-24 2021-09-29 ジェネインフォセック、インコーポレイテッド 核酸内に存在する情報の秘匿化
WO2018118971A1 (en) 2016-12-19 2018-06-28 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
US10845308B2 (en) 2016-12-22 2020-11-24 Quantum-Si Incorporated Integrated photodetector with direct binning pixel
WO2018191857A1 (zh) * 2017-04-18 2018-10-25 深圳华大智造科技有限公司 Phi29 DNA聚合酶及其编码基因与应用
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CA3059211A1 (en) 2017-05-05 2018-11-08 Quantum-Si Incorporated Substrates having modified surface reactivity and antifouling properties in biological reactions
EP3625240A4 (de) 2017-05-18 2021-01-27 Ultima Genomics, Inc. Sequenzierung mit nichtnatürlichen nukleotiden
US11186862B2 (en) 2017-06-20 2021-11-30 Bio-Rad Laboratories, Inc. MDA using bead oligonucleotide
CN117106037B (zh) 2017-06-30 2025-07-25 弗拉芒区生物技术研究所 新颖蛋白孔
BR112020000826A2 (pt) 2017-07-24 2020-07-21 Quantum-Si Incorporated instrumento bio-optoeletrônico maciçamente paralelo portátil
US11162138B2 (en) 2017-10-30 2021-11-02 Pacific Biosciences Of California, Inc. Multi-amplitude modular labeled compounds
US10907205B2 (en) 2017-11-02 2021-02-02 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
EP3729090A4 (de) 2017-12-22 2021-09-22 Pacific Biosciences Of California, Inc. Modifizierte biotin-bindende proteine zur immobilisierung
CN119614672A (zh) 2018-01-31 2025-03-14 生物辐射实验室股份有限公司 用于解卷积分区条码的方法和组合物
EP3765632A4 (de) 2018-03-13 2021-12-08 Sarmal, Inc. Verfahren zur einzelmolekülsequenzierung
US11512002B2 (en) 2018-04-18 2022-11-29 University Of Virginia Patent Foundation Silica materials and methods of making thereof
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
AU2019288394B2 (en) 2018-06-22 2025-01-30 Quantum-Si Incorporated Integrated photodetector with charge storage bin of varied detection time
WO2020006421A1 (en) 2018-06-29 2020-01-02 Pacific Biosciences Of California, Inc. Methods and compositions for delivery of molecules and complexes to reaction sites
CN113166807B (zh) 2018-08-20 2024-06-04 生物辐射实验室股份有限公司 通过分区中条码珠共定位生成核苷酸序列
EP3674702A1 (de) 2018-12-27 2020-07-01 Imec VZW Verfahren zur sequenzierung eines polynukleotids mittels eines biofet
US11773422B2 (en) 2019-08-16 2023-10-03 Microsoft Technology Licensing, Llc Regulation of polymerase using cofactor oxidation states
US11795450B2 (en) 2019-09-06 2023-10-24 Microsoft Technology Licensing, Llc Array-based enzymatic oligonucleotide synthesis
BR112022006682A2 (pt) 2019-10-11 2022-07-12 Quantum Si Inc Modificação de superfície na fase de vapor
CN110713519A (zh) * 2019-11-02 2020-01-21 深圳市安帝宝科技有限公司 一种11-脱氢血栓烷b2抗原及其制备方法
US12006535B2 (en) 2019-11-19 2024-06-11 Sarmal, Inc. Methods and devices for detecting SARS-COV-2
EP4061962A4 (de) 2019-11-19 2024-10-09 Sarmal, Inc. Verfahren und vorrichtungen zum nachweis von sars-cov-2
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
US11702683B2 (en) 2020-05-28 2023-07-18 Microsoft Technology Licensing, Llc De novo polynucleotide synthesis with substrate-bound polymerase
CA3195982A1 (en) 2020-10-23 2022-04-28 Spindle Biotech Inc. Compositions and methods for rna synthesis
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
WO2025244989A1 (en) 2024-05-21 2025-11-27 Pacific Biosciences Of California, Inc. Selectively functionalized nanoscale wells
WO2026019720A1 (en) 2024-07-15 2026-01-22 Pacific Biosciences Of California, Inc. Improved loading of molecules and complexes to reaction sites

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198543A (en) * 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5496934A (en) * 1993-04-14 1996-03-05 Yissum Research Development Company Of The Hebrew University Of Jerusalem Nucleic acids encoding a cellulose binding domain
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US6383483B1 (en) * 1995-01-06 2002-05-07 Désiré José Collen Staphylokinase derivatives with cysteine substitutions
US5998580A (en) * 1995-10-13 1999-12-07 Fay; Frederick F. Photosensitive caged macromolecules
US6261797B1 (en) * 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
US5972603A (en) * 1996-02-09 1999-10-26 President And Fellows Of Harvard College DNA polymerase with modified processivity
US5736373A (en) * 1996-09-23 1998-04-07 Becton, Dickinson And Company Thermostable DNA polymerase from Bacillus pallidus
AU9312598A (en) * 1997-09-10 1999-03-29 Allegheny University Of The Health Sciences Inhibitors of collagen assembly
US6346379B1 (en) * 1997-09-11 2002-02-12 F. Hoffman-La Roche Ag Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
US6077940A (en) * 1997-12-24 2000-06-20 Genentech, Inc. Free solution ligand interaction molecular separation method
DE19810879A1 (de) 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
AR021833A1 (es) * 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
EP1159453B1 (de) 1999-03-10 2008-05-28 ASM Scientific, Inc. Methode zur direkten sequenzierung von nukleinsäuren
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US20030077808A1 (en) * 2000-01-31 2003-04-24 Rosen Craig A. Nucleic acids, proteins, and antibodies
US20030140369A1 (en) * 2000-02-29 2003-07-24 Pioneer Hi-Bred International, Inc. Novel defense induced multi-drug resistance genes and uses thereof
US20010039016A1 (en) * 2000-03-27 2001-11-08 Waldman Scott A. Use of expression profiling for identifying molecular markers useful for diagnosis of metastatic cancer
US6917726B2 (en) * 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US20030036181A1 (en) * 2000-06-30 2003-02-20 Okkels Jens Sigurd Peptide extended glycosylated polypeptides
DE10034804A1 (de) * 2000-07-18 2002-01-31 Bayer Ag Verwendung von VLCFAE zum Identifizieren von herbizid wirksamen Verbindungen
US7238505B2 (en) * 2000-10-04 2007-07-03 Ahram Biosystems Inc. Immobilized DNA polymerase
WO2002029027A1 (en) * 2000-10-04 2002-04-11 Ahram Biosystems Inc. Immobilized dna polymerase
AU2002258997A1 (en) * 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
US7205106B1 (en) * 2001-07-20 2007-04-17 Roche Molecular Systems, Inc. Association of polymorphisms in IL4-related genes with autoimmune disease
US7041812B2 (en) * 2001-08-29 2006-05-09 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US7560254B2 (en) * 2001-08-29 2009-07-14 Ge Healthcare Bio-Sciences Corp. Allele specific primer extension
US20050187718A1 (en) * 2001-09-21 2005-08-25 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from Streptococcus pneumoniae
US7745116B2 (en) * 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
US7405281B2 (en) * 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
CA2633524A1 (en) * 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation

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EP1963530A4 (de) 2009-08-05
EP1963530A2 (de) 2008-09-03
CA2633476C (en) 2015-04-21
AU2006331512A1 (en) 2007-07-05
CA2633476A1 (en) 2007-07-05
WO2007075987A2 (en) 2007-07-05
US8936926B2 (en) 2015-01-20
EP1963530B1 (de) 2011-07-27
AU2006331512B2 (en) 2012-02-23
WO2007075987A3 (en) 2009-01-29
US20080199932A1 (en) 2008-08-21
US8323939B2 (en) 2012-12-04

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