ATE527371T1 - Expression löslicher, aktiver, eukaryotischer glucosyltransferasen in prokaryotischen organismen - Google Patents
Expression löslicher, aktiver, eukaryotischer glucosyltransferasen in prokaryotischen organismenInfo
- Publication number
- ATE527371T1 ATE527371T1 AT06748724T AT06748724T ATE527371T1 AT E527371 T1 ATE527371 T1 AT E527371T1 AT 06748724 T AT06748724 T AT 06748724T AT 06748724 T AT06748724 T AT 06748724T AT E527371 T1 ATE527371 T1 AT E527371T1
- Authority
- AT
- Austria
- Prior art keywords
- active
- soluble
- eukaryotic
- expression
- prokaryotic organisms
- Prior art date
Links
- 102000000340 Glucosyltransferases Human genes 0.000 title 1
- 108010055629 Glucosyltransferases Proteins 0.000 title 1
- 108700023372 Glycosyltransferases Proteins 0.000 abstract 1
- 102000045442 glycosyltransferase activity proteins Human genes 0.000 abstract 1
- 108700014210 glycosyltransferase activity proteins Proteins 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 230000001590 oxidative effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US66539605P | 2005-03-24 | 2005-03-24 | |
| US66889905P | 2005-04-05 | 2005-04-05 | |
| US73240905P | 2005-10-31 | 2005-10-31 | |
| PCT/US2006/011065 WO2006102652A2 (en) | 2005-03-24 | 2006-03-24 | Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE527371T1 true ATE527371T1 (de) | 2011-10-15 |
Family
ID=37024713
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06748724T ATE527371T1 (de) | 2005-03-24 | 2006-03-24 | Expression löslicher, aktiver, eukaryotischer glucosyltransferasen in prokaryotischen organismen |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US20100143969A1 (de) |
| EP (1) | EP1863921B1 (de) |
| JP (1) | JP5235657B2 (de) |
| KR (1) | KR20080021590A (de) |
| AT (1) | ATE527371T1 (de) |
| AU (1) | AU2006226782B2 (de) |
| CA (1) | CA2602329C (de) |
| ES (1) | ES2374935T3 (de) |
| MX (1) | MX2007011801A (de) |
| WO (1) | WO2006102652A2 (de) |
Families Citing this family (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002236234B2 (en) * | 2001-03-06 | 2007-12-20 | Phyton Holdings, LLC. | Plant cell having animal-type sugar chain adding function |
| US7214660B2 (en) * | 2001-10-10 | 2007-05-08 | Neose Technologies, Inc. | Erythropoietin: remodeling and glycoconjugation of erythropoietin |
| US7173003B2 (en) | 2001-10-10 | 2007-02-06 | Neose Technologies, Inc. | Granulocyte colony stimulating factor: remodeling and glycoconjugation of G-CSF |
| NZ542094A (en) | 2003-03-14 | 2008-12-24 | Neose Technologies Inc | Branched polymer conjugates comprising a peptide and water-soluble polymer chains |
| PL1615945T3 (pl) | 2003-04-09 | 2012-03-30 | Ratiopharm Gmbh | Sposoby glikopegylacji i białka/peptydy wytwarzane tymi sposobami |
| US8791070B2 (en) | 2003-04-09 | 2014-07-29 | Novo Nordisk A/S | Glycopegylated factor IX |
| US9005625B2 (en) | 2003-07-25 | 2015-04-14 | Novo Nordisk A/S | Antibody toxin conjugates |
| US8633157B2 (en) | 2003-11-24 | 2014-01-21 | Novo Nordisk A/S | Glycopegylated erythropoietin |
| US20080305992A1 (en) | 2003-11-24 | 2008-12-11 | Neose Technologies, Inc. | Glycopegylated erythropoietin |
| US20060040856A1 (en) | 2003-12-03 | 2006-02-23 | Neose Technologies, Inc. | Glycopegylated factor IX |
| NZ548123A (en) | 2004-01-08 | 2010-05-28 | Novo Nordisk As | O-linked glycosylation of peptides |
| US20080300173A1 (en) * | 2004-07-13 | 2008-12-04 | Defrees Shawn | Branched Peg Remodeling and Glycosylation of Glucagon-Like Peptides-1 [Glp-1] |
| WO2006031811A2 (en) | 2004-09-10 | 2006-03-23 | Neose Technologies, Inc. | Glycopegylated interferon alpha |
| WO2006050247A2 (en) | 2004-10-29 | 2006-05-11 | Neose Technologies, Inc. | Remodeling and glycopegylation of fibroblast growth factor (fgf) |
| US9029331B2 (en) | 2005-01-10 | 2015-05-12 | Novo Nordisk A/S | Glycopegylated granulocyte colony stimulating factor |
| US20100143969A1 (en) * | 2005-03-24 | 2010-06-10 | Neose Technologies, Inc. | Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms |
| WO2006121569A2 (en) | 2005-04-08 | 2006-11-16 | Neose Technologies, Inc. | Compositions and methods for the preparation of protease resistant human growth hormone glycosylation mutants |
| EP2975135A1 (de) | 2005-05-25 | 2016-01-20 | Novo Nordisk A/S | Glykopegylierter Faktor IX |
| US20070105755A1 (en) * | 2005-10-26 | 2007-05-10 | Neose Technologies, Inc. | One pot desialylation and glycopegylation of therapeutic peptides |
| WO2007056191A2 (en) | 2005-11-03 | 2007-05-18 | Neose Technologies, Inc. | Nucleotide sugar purification using membranes |
| US8198045B2 (en) * | 2006-04-19 | 2012-06-12 | Biogenerix Ag | Expression of O-glycosylated therapeutic proteins in prokaryotic microorganisms |
| EP2049144B8 (de) | 2006-07-21 | 2015-02-18 | ratiopharm GmbH | Glycosylierung von peptiden über o-verbundene glycosylierungssequenzen |
| EP2054521A4 (de) | 2006-10-03 | 2012-12-19 | Novo Nordisk As | Verfahren zur reinigung von polypeptid-konjugate |
| AU2008227024A1 (en) * | 2007-03-07 | 2008-09-18 | Glycofi, Inc. | Production of glycoproteins with modified fucosylation |
| HRP20130382T1 (hr) | 2007-04-03 | 2013-05-31 | Biogenerix Ag | Postupci lijeäśenja pomoä†u glikopegiliranog g-csf |
| WO2008125972A2 (en) | 2007-04-17 | 2008-10-23 | Plant Research International B.V. | Mammalian-type glycosylation in plants by expression of non-mammalian glycosyltransferases |
| JP2008278761A (ja) * | 2007-05-08 | 2008-11-20 | Tokai Univ | 新規β−ガラクトシドα2,6−シアル酸転移酵素 |
| WO2008143944A2 (en) * | 2007-05-14 | 2008-11-27 | Government Of The Usa, As Represented By The Secretary, Department Of Health And Human Services | Methods of glycosylation and bioconjugation |
| CA2690611C (en) | 2007-06-12 | 2015-12-08 | Novo Nordisk A/S | Improved process for the production of nucleotide sugars |
| US8207112B2 (en) | 2007-08-29 | 2012-06-26 | Biogenerix Ag | Liquid formulation of G-CSF conjugate |
| CN107119095B (zh) * | 2008-01-03 | 2022-07-05 | 康乃尔研究基金会有限公司 | 原核生物中的糖基化蛋白表达 |
| PL2257311T3 (pl) | 2008-02-27 | 2014-09-30 | Novo Nordisk As | Koniugaty cząsteczek czynnika VIII |
| NO2501406T3 (de) | 2009-11-19 | 2018-05-19 | ||
| EP2536753B1 (de) | 2010-02-16 | 2017-12-20 | Novo Nordisk A/S | Faktor-VIII-Moleküle mit reduzierter VWF-Bindung |
| JP2013519699A (ja) | 2010-02-16 | 2013-05-30 | ノヴォ ノルディスク アー/エス | 因子viii融合タンパク質 |
| JP5956982B2 (ja) | 2010-05-27 | 2016-07-27 | メルク・シャープ・エンド・ドーム・コーポレイション | 改善された特性を有する抗体の製造方法 |
| JP2013532176A (ja) | 2010-07-15 | 2013-08-15 | ノヴォ ノルディスク アー/エス | 安定化させた第viii因子バリアント |
| EP2415779B1 (de) | 2010-08-02 | 2015-01-21 | ratiopharm GmbH | Verfahren zur Herstellung und Reinigung einer aktiven löslichen Sialyltransferase |
| AU2012258907A1 (en) | 2011-05-25 | 2013-11-07 | Merck Sharp & Dohme Corp. | Method for preparing Fc-containing polypeptides having improved properties |
| EP2852610B1 (de) | 2012-05-23 | 2018-07-11 | Glykos Finland Oy | Herstellung von fucosylierten glykoproteinen |
| EP2877589B1 (de) | 2012-07-26 | 2024-03-06 | Momenta Pharmaceuticals, Inc. | Glykoproteine mit entzündungshemmenden eigenschaften |
| SG11201508347YA (en) * | 2013-03-14 | 2015-11-27 | Glycobia Inc | Oligosaccharide compositions, glycoproteins and methods to produce the same in prokaryotes |
| PL2991683T3 (pl) | 2013-05-02 | 2020-03-31 | Glykos Finland Oy | Koniugaty glikoproteiny lub glikanu z toksycznym ładunkiem |
| CA2954534C (en) * | 2014-07-07 | 2023-04-04 | Targazyme, Inc. | Manufacture and cryopreservation of fucosylated cells for therapeutic use |
| SG11201700446XA (en) | 2014-07-21 | 2017-02-27 | Glykos Finland Oy | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
| US9616114B1 (en) | 2014-09-18 | 2017-04-11 | David Gordon Bermudes | Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| WO2017117539A1 (en) | 2015-12-30 | 2017-07-06 | Northwestern University | Cell-free glycoprotein synthesis (cfgps) in prokaryotic cell lysates enriched with components for glycosylation |
| US10829795B2 (en) | 2016-07-14 | 2020-11-10 | Northwestern University | Method for rapid in vitro synthesis of glycoproteins via recombinant production of N-glycosylated proteins in prokaryotic cell lysates |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| WO2019035916A1 (en) | 2017-08-15 | 2019-02-21 | Northwestern University | DESIGN OF PROTEIN GLYCOSYLATION SITES BY RAPID EXPRESSION AND CHARACTERIZATION OF N-GLYCOSYLTRANSFERASES |
| US11530432B2 (en) | 2018-03-19 | 2022-12-20 | Northwestern University | Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates |
| WO2019204346A1 (en) | 2018-04-16 | 2019-10-24 | Northwestern University | METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES |
| US12098433B2 (en) | 2018-08-03 | 2024-09-24 | Northwestern University | On demand, portable, cell-free molecular sensing platform |
| CN113646438A (zh) | 2019-01-11 | 2021-11-12 | 西北大学 | 在原核生物细胞裂解物中合成生物缀合物疫苗 |
| US12325884B2 (en) | 2019-03-04 | 2025-06-10 | Northwestern University | Riboswitch-based fluoride sensing in cell-free extract |
| US12226410B2 (en) | 2019-10-18 | 2025-02-18 | Northwestern University | Methods for enhancing cellular clearance of pathological molecules via activation of the cellular protein ykt6 |
| CN115135771A (zh) | 2019-10-25 | 2022-09-30 | 西北大学 | 用于富集膜泡和增加糖蛋白产量的无细胞提取物制备方案 |
| MX2022010467A (es) * | 2020-02-25 | 2022-12-15 | Momenta Pharmaceuticals Inc | Enzimas para la sialilación de glicanos. |
| CA3174533A1 (en) * | 2020-03-05 | 2021-09-10 | Momenta Pharmaceuticals, Inc. | Methods of making hyper-sialylated immunoglobulin |
| CN111534495B (zh) * | 2020-05-14 | 2022-06-17 | 江南大学 | 一种提高重组n-乙酰葡糖胺转移酶ii可溶性表达的方法 |
| WO2022207676A2 (en) | 2021-03-29 | 2022-10-06 | Imperial College Innovations Limited | Methods and polynucleotides |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5858751A (en) * | 1992-03-09 | 1999-01-12 | The Regents Of The University Of California | Compositions and methods for producing sialyltransferases |
| GB9323225D0 (en) * | 1993-11-10 | 1994-01-05 | Unilever Plc | Novel plant enzyme |
| US5639635A (en) | 1994-11-03 | 1997-06-17 | Genentech, Inc. | Process for bacterial production of polypeptides |
| EP0786382A1 (de) | 1996-01-26 | 1997-07-30 | Morton International, Inc. | Beifahrerseitiges Gaskissen |
| AU2660397A (en) | 1996-04-05 | 1997-10-29 | Board Of Regents, The University Of Texas System | Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells |
| JP2001507215A (ja) | 1997-01-16 | 2001-06-05 | サイテル コーポレイション | 組換え糖タンパク質のインビトロでの実用的なシアリル化 |
| WO1998035049A1 (en) * | 1997-02-07 | 1998-08-13 | Oriental Yeast Co., Ltd. | Recombinant yeast pdi and process for preparing the same |
| JP3344618B2 (ja) * | 1997-06-20 | 2002-11-11 | 株式会社エイチ・エス・ピー研究所 | シャペロン発現プラスミド |
| JPH11137247A (ja) | 1997-11-10 | 1999-05-25 | Toyobo Co Ltd | β1,4−ガラクトース転移酵素の製造法 |
| JPH11253163A (ja) | 1998-03-11 | 1999-09-21 | Toyobo Co Ltd | シアル酸転移酵素の製造法 |
| US6037730A (en) | 1998-11-12 | 2000-03-14 | Eaton Corporation | Degraded electronic throttle operation method and system |
| US6949372B2 (en) | 1999-03-02 | 2005-09-27 | The Johns Hopkins University | Engineering intracellular sialylation pathways |
| JP2001178453A (ja) | 1999-12-24 | 2001-07-03 | Toyobo Co Ltd | N−アセチルグルコサミニルトランスフェラーゼの製造方法 |
| US7338932B2 (en) * | 2000-05-11 | 2008-03-04 | Glycozym Aps | Methods of modulating functions of polypeptide GalNAc-transferases and of screening test substances to find agents herefor, pharmaceutical compositions comprising such agents and the use of such agents for preparing medicaments |
| ES2411007T3 (es) * | 2001-10-10 | 2013-07-04 | Novo Nordisk A/S | Remodelación y glicoconjugación de péptidos |
| US7179617B2 (en) | 2001-10-10 | 2007-02-20 | Neose Technologies, Inc. | Factor IX: remolding and glycoconjugation of Factor IX |
| EP1477028A2 (de) * | 2002-02-21 | 2004-11-17 | BRITISH TELECOMMUNICATIONS public limited company | Videoverarbeitung |
| EP1487966B1 (de) * | 2002-03-23 | 2006-12-13 | Research Development Foundation | Sekretion von proteinen mit mehreren disulfidbindungen in bakterien und verwendungen davon |
| US7569376B2 (en) * | 2002-05-03 | 2009-08-04 | Neose Technologies, Inc. | Fucosyltransferase fusion protein |
| JP2004016116A (ja) | 2002-06-17 | 2004-01-22 | Toyobo Co Ltd | β1,4−N−アセチルガラクトサミニルトランスフェラーゼ活性を有する融合蛋白質及びその製造方法 |
| JP2004016117A (ja) | 2002-06-17 | 2004-01-22 | Toyobo Co Ltd | β1,3−ガラクトシルトランスフェラーゼ活性を有する融合蛋白質及びその製造方法 |
| NZ543553A (en) * | 2003-05-27 | 2008-12-24 | Univ York | Bioreactor containing cells expressing glycosyltransferase nucleic acids |
| JP2005046030A (ja) | 2003-07-31 | 2005-02-24 | Toyobo Co Ltd | I分岐形成β1,6−N−アセチルグルコサミニルトランスフェラーゼ活性を有する融合タンパク質及びその製造方法 |
| JP2005046031A (ja) | 2003-07-31 | 2005-02-24 | Toyobo Co Ltd | α1,3−フコシルトランスフェラーゼVI活性を有する融合タンパク質及びその製造方法 |
| AU2005222782B2 (en) * | 2004-02-04 | 2010-06-17 | Ratiopharm Gmbh | Methods of refolding mammalian glycosyltransferases |
| US20100143969A1 (en) | 2005-03-24 | 2010-06-10 | Neose Technologies, Inc. | Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms |
-
2006
- 2006-03-24 US US11/909,599 patent/US20100143969A1/en not_active Abandoned
- 2006-03-24 JP JP2008503276A patent/JP5235657B2/ja not_active Expired - Fee Related
- 2006-03-24 WO PCT/US2006/011065 patent/WO2006102652A2/en not_active Ceased
- 2006-03-24 EP EP06748724A patent/EP1863921B1/de not_active Expired - Lifetime
- 2006-03-24 ES ES06748724T patent/ES2374935T3/es not_active Expired - Lifetime
- 2006-03-24 MX MX2007011801A patent/MX2007011801A/es not_active Application Discontinuation
- 2006-03-24 AT AT06748724T patent/ATE527371T1/de not_active IP Right Cessation
- 2006-03-24 US US11/388,595 patent/US8137928B2/en not_active Expired - Fee Related
- 2006-03-24 AU AU2006226782A patent/AU2006226782B2/en not_active Ceased
- 2006-03-24 KR KR1020077024354A patent/KR20080021590A/ko not_active Withdrawn
- 2006-03-24 CA CA2602329A patent/CA2602329C/en not_active Expired - Fee Related
-
2011
- 2011-03-28 US US13/073,445 patent/US20110223646A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CA2602329A1 (en) | 2006-09-28 |
| CA2602329C (en) | 2016-08-09 |
| MX2007011801A (es) | 2008-02-19 |
| US20060234345A1 (en) | 2006-10-19 |
| JP2008533992A (ja) | 2008-08-28 |
| AU2006226782B2 (en) | 2011-12-08 |
| EP1863921A4 (de) | 2009-04-08 |
| JP5235657B2 (ja) | 2013-07-10 |
| US20110223646A1 (en) | 2011-09-15 |
| ES2374935T3 (es) | 2012-02-23 |
| KR20080021590A (ko) | 2008-03-07 |
| US8137928B2 (en) | 2012-03-20 |
| US20100143969A1 (en) | 2010-06-10 |
| AU2006226782A1 (en) | 2006-09-28 |
| WO2006102652A2 (en) | 2006-09-28 |
| EP1863921B1 (de) | 2011-10-05 |
| WO2006102652A3 (en) | 2007-05-24 |
| EP1863921A2 (de) | 2007-12-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |