ATE527371T1 - Expression löslicher, aktiver, eukaryotischer glucosyltransferasen in prokaryotischen organismen - Google Patents
Expression löslicher, aktiver, eukaryotischer glucosyltransferasen in prokaryotischen organismenInfo
- Publication number
- ATE527371T1 ATE527371T1 AT06748724T AT06748724T ATE527371T1 AT E527371 T1 ATE527371 T1 AT E527371T1 AT 06748724 T AT06748724 T AT 06748724T AT 06748724 T AT06748724 T AT 06748724T AT E527371 T1 ATE527371 T1 AT E527371T1
- Authority
- AT
- Austria
- Prior art keywords
- active
- soluble
- eukaryotic
- expression
- prokaryotic organisms
- Prior art date
Links
- 102000000340 Glucosyltransferases Human genes 0.000 title 1
- 108010055629 Glucosyltransferases Proteins 0.000 title 1
- 108700023372 Glycosyltransferases Proteins 0.000 abstract 1
- 102000045442 glycosyltransferase activity proteins Human genes 0.000 abstract 1
- 108700014210 glycosyltransferase activity proteins Proteins 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 230000001590 oxidative effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US66539605P | 2005-03-24 | 2005-03-24 | |
| US66889905P | 2005-04-05 | 2005-04-05 | |
| US73240905P | 2005-10-31 | 2005-10-31 | |
| PCT/US2006/011065 WO2006102652A2 (en) | 2005-03-24 | 2006-03-24 | Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE527371T1 true ATE527371T1 (de) | 2011-10-15 |
Family
ID=37024713
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06748724T ATE527371T1 (de) | 2005-03-24 | 2006-03-24 | Expression löslicher, aktiver, eukaryotischer glucosyltransferasen in prokaryotischen organismen |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US20100143969A1 (de) |
| EP (1) | EP1863921B1 (de) |
| JP (1) | JP5235657B2 (de) |
| KR (1) | KR20080021590A (de) |
| AT (1) | ATE527371T1 (de) |
| AU (1) | AU2006226782B2 (de) |
| CA (1) | CA2602329C (de) |
| ES (1) | ES2374935T3 (de) |
| MX (1) | MX2007011801A (de) |
| WO (1) | WO2006102652A2 (de) |
Families Citing this family (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MXPA03008001A (es) * | 2001-03-06 | 2004-10-15 | Dow Chemical Co | Celula vegetal que tiene funcion de adicion de cadena de azucar tipo animal. |
| US7173003B2 (en) | 2001-10-10 | 2007-02-06 | Neose Technologies, Inc. | Granulocyte colony stimulating factor: remodeling and glycoconjugation of G-CSF |
| US7214660B2 (en) * | 2001-10-10 | 2007-05-08 | Neose Technologies, Inc. | Erythropoietin: remodeling and glycoconjugation of erythropoietin |
| JP2006523211A (ja) | 2003-03-14 | 2006-10-12 | ネオス テクノロジーズ インコーポレイテッド | 分岐水溶性ポリマーとその複合物 |
| US8791070B2 (en) | 2003-04-09 | 2014-07-29 | Novo Nordisk A/S | Glycopegylated factor IX |
| NZ542586A (en) | 2003-04-09 | 2009-11-27 | Biogenerix Ag | Method of forming a covalent conjugate between a G-CSF peptide and PEG |
| WO2006127896A2 (en) | 2005-05-25 | 2006-11-30 | Neose Technologies, Inc. | Glycopegylated factor ix |
| US9005625B2 (en) | 2003-07-25 | 2015-04-14 | Novo Nordisk A/S | Antibody toxin conjugates |
| US20080305992A1 (en) | 2003-11-24 | 2008-12-11 | Neose Technologies, Inc. | Glycopegylated erythropoietin |
| US8633157B2 (en) | 2003-11-24 | 2014-01-21 | Novo Nordisk A/S | Glycopegylated erythropoietin |
| US20060040856A1 (en) | 2003-12-03 | 2006-02-23 | Neose Technologies, Inc. | Glycopegylated factor IX |
| US7338933B2 (en) | 2004-01-08 | 2008-03-04 | Neose Technologies, Inc. | O-linked glycosylation of peptides |
| WO2006010143A2 (en) * | 2004-07-13 | 2006-01-26 | Neose Technologies, Inc. | Branched peg remodeling and glycosylation of glucagon-like peptide-1 [glp-1] |
| EP1799249A2 (de) | 2004-09-10 | 2007-06-27 | Neose Technologies, Inc. | Glycopegyliertes interferon alpha |
| EP3061461A1 (de) | 2004-10-29 | 2016-08-31 | ratiopharm GmbH | Remodellierung und glykopegylierung von fibroblasten-wachstumsfaktor (fgf) |
| US9029331B2 (en) | 2005-01-10 | 2015-05-12 | Novo Nordisk A/S | Glycopegylated granulocyte colony stimulating factor |
| WO2006102652A2 (en) * | 2005-03-24 | 2006-09-28 | Neose Technologies, Inc. | Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms |
| EP1871795A4 (de) | 2005-04-08 | 2010-03-31 | Biogenerix Ag | Zusammensetzungen und verfahren zur herstellung von glycosylierungsmutanten eines proteaseresistenten menschlichen wachstumshormons |
| US20070105755A1 (en) * | 2005-10-26 | 2007-05-10 | Neose Technologies, Inc. | One pot desialylation and glycopegylation of therapeutic peptides |
| WO2007056191A2 (en) | 2005-11-03 | 2007-05-18 | Neose Technologies, Inc. | Nucleotide sugar purification using membranes |
| EP2013357A4 (de) * | 2006-04-19 | 2012-02-22 | Biogenerix Ag | Expression o-glycosylierter therapeutischer proteine in prokaryotischen mikroorganismen |
| US20080242607A1 (en) | 2006-07-21 | 2008-10-02 | Neose Technologies, Inc. | Glycosylation of peptides via o-linked glycosylation sequences |
| US8969532B2 (en) | 2006-10-03 | 2015-03-03 | Novo Nordisk A/S | Methods for the purification of polypeptide conjugates comprising polyalkylene oxide using hydrophobic interaction chromatography |
| CA2679732A1 (en) * | 2007-03-07 | 2008-09-18 | Glycofi, Inc. | Production of glycoproteins with modified fucosylation |
| CN101796063B (zh) | 2007-04-03 | 2017-03-22 | 拉蒂奥法姆有限责任公司 | 使用糖聚乙二醇化g‑csf的治疗方法 |
| NZ580510A (en) | 2007-04-17 | 2011-06-30 | Stichting Dienst Landbouwkundi | Mammalian-type glycosylation in plants by expression of non-mammalian glycosyltransferases |
| JP2008278761A (ja) * | 2007-05-08 | 2008-11-20 | Tokai Univ | 新規β−ガラクトシドα2,6−シアル酸転移酵素 |
| WO2008143944A2 (en) * | 2007-05-14 | 2008-11-27 | Government Of The Usa, As Represented By The Secretary, Department Of Health And Human Services | Methods of glycosylation and bioconjugation |
| US9493499B2 (en) | 2007-06-12 | 2016-11-15 | Novo Nordisk A/S | Process for the production of purified cytidinemonophosphate-sialic acid-polyalkylene oxide (CMP-SA-PEG) as modified nucleotide sugars via anion exchange chromatography |
| US8207112B2 (en) | 2007-08-29 | 2012-06-26 | Biogenerix Ag | Liquid formulation of G-CSF conjugate |
| DK2240595T4 (da) * | 2008-01-03 | 2019-10-07 | Cornell Res Foundation Inc | Glykosyleret proteinekspression i prokaryoter |
| DK2257311T3 (da) | 2008-02-27 | 2014-06-30 | Novo Nordisk As | Konjugerede Faktor VIII-Molekyler |
| TR201803015T4 (tr) * | 2009-11-19 | 2018-03-21 | Glaxosmithkline Biologicals Sa | Prokaryotik hücrelerde immünojenik polisakaridler üreten biyosentetik sistem. |
| US9493543B2 (en) | 2010-02-16 | 2016-11-15 | Novo Nordisk A/S | Factor VIII fusion protein |
| JP5914363B2 (ja) | 2010-02-16 | 2016-05-11 | ノヴォ ノルディスク アー/エス | 低減されたvwf結合を有する因子viii分子 |
| RU2604811C2 (ru) | 2010-05-27 | 2016-12-10 | Мерк Шарп Энд Домэ Корп. | Способ получения антител с улучшенными свойствами |
| US20130183280A1 (en) | 2010-07-15 | 2013-07-18 | Novo Nordisk A/S | Stabilized factor viii variants |
| PT2415779E (pt) | 2010-08-02 | 2015-05-13 | Ratiopharm Gmbh | Processo de produção e purificação de uma sialiltransferase solúvel activa |
| JP2014517846A (ja) | 2011-05-25 | 2014-07-24 | メルク・シャープ・アンド・ドーム・コーポレーション | 改善された特性を有するFc含有ポリペプチドの製造方法 |
| DK2852610T3 (en) | 2012-05-23 | 2018-09-03 | Glykos Finland Oy | PRODUCTION OF FUCOSYLED GLYCOPROTEIN |
| EP2877589B1 (de) | 2012-07-26 | 2024-03-06 | Momenta Pharmaceuticals, Inc. | Glykoproteine mit entzündungshemmenden eigenschaften |
| JP2016518114A (ja) * | 2013-03-14 | 2016-06-23 | グリコビア,インコーポレイテッド | オリゴ糖組成物、糖タンパク質および原核生物においてそれらを産生する方法 |
| ES2759503T3 (es) | 2013-05-02 | 2020-05-11 | Glykos Finland Oy | Conjugados de una glicoproteína o un glicano con una carga útil tóxica |
| US20170121673A1 (en) * | 2014-07-07 | 2017-05-04 | Targazyme, Inc. | Manufacture and Cryopreservation of Fucosylated Cells for Therapeutic Use |
| AU2015293949B2 (en) | 2014-07-21 | 2019-07-25 | Teknologian Tutkimuskeskus Vtt Oy | Production of glycoproteins with mammalian-like N-glycans in filamentous fungi |
| US9616114B1 (en) | 2014-09-18 | 2017-04-11 | David Gordon Bermudes | Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| WO2017117539A1 (en) | 2015-12-30 | 2017-07-06 | Northwestern University | Cell-free glycoprotein synthesis (cfgps) in prokaryotic cell lysates enriched with components for glycosylation |
| US10829795B2 (en) | 2016-07-14 | 2020-11-10 | Northwestern University | Method for rapid in vitro synthesis of glycoproteins via recombinant production of N-glycosylated proteins in prokaryotic cell lysates |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| WO2019035916A1 (en) | 2017-08-15 | 2019-02-21 | Northwestern University | DESIGN OF PROTEIN GLYCOSYLATION SITES BY RAPID EXPRESSION AND CHARACTERIZATION OF N-GLYCOSYLTRANSFERASES |
| US11530432B2 (en) | 2018-03-19 | 2022-12-20 | Northwestern University | Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates |
| US11725224B2 (en) | 2018-04-16 | 2023-08-15 | Northwestern University | Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates |
| WO2020072127A2 (en) | 2018-08-03 | 2020-04-09 | Northwestern University | On demand, portable, cell-free molecular sensing platform |
| WO2020146814A1 (en) | 2019-01-11 | 2020-07-16 | Northwestern University | Bioconjugate vaccines' synthesis in prokaryotic cell lysates |
| WO2020185451A2 (en) | 2019-03-04 | 2020-09-17 | Northwestern University | Riboswitch-based fluoride sensing in cell-free extract |
| US12226410B2 (en) | 2019-10-18 | 2025-02-18 | Northwestern University | Methods for enhancing cellular clearance of pathological molecules via activation of the cellular protein ykt6 |
| AU2020371793A1 (en) | 2019-10-25 | 2022-06-02 | Northwestern University | Cell-free extract preparation protocol for enrichment of membrane vesicles and increased glycoprotein yields |
| AU2021226352A1 (en) * | 2020-02-25 | 2022-09-29 | Momenta Pharmaceuticals, Inc. | Enzymes for sialylation of glycans |
| MX2022010826A (es) * | 2020-03-05 | 2022-12-02 | Momenta Pharmaceuticals Inc | Métodos para elaborar inmunoglobulina hipersialilada. |
| CN111534495B (zh) * | 2020-05-14 | 2022-06-17 | 江南大学 | 一种提高重组n-乙酰葡糖胺转移酶ii可溶性表达的方法 |
| WO2022207676A2 (en) | 2021-03-29 | 2022-10-06 | Imperial College Innovations Limited | Methods and polynucleotides |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5858751A (en) * | 1992-03-09 | 1999-01-12 | The Regents Of The University Of California | Compositions and methods for producing sialyltransferases |
| GB9323225D0 (en) * | 1993-11-10 | 1994-01-05 | Unilever Plc | Novel plant enzyme |
| US5639635A (en) | 1994-11-03 | 1997-06-17 | Genentech, Inc. | Process for bacterial production of polypeptides |
| EP0786382A1 (de) | 1996-01-26 | 1997-07-30 | Morton International, Inc. | Beifahrerseitiges Gaskissen |
| AU2660397A (en) | 1996-04-05 | 1997-10-29 | Board Of Regents, The University Of Texas System | Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells |
| NZ336780A (en) | 1997-01-16 | 2000-12-22 | Neose Technologies Inc | In vitro sialylation of glycoproteins |
| EP0974665A4 (de) * | 1997-02-07 | 2003-02-05 | Oriental Yeast Co Ltd | Rekombinantes pdi aus hefe und verfahren zu dessen herstellung |
| JP3344618B2 (ja) * | 1997-06-20 | 2002-11-11 | 株式会社エイチ・エス・ピー研究所 | シャペロン発現プラスミド |
| JPH11137247A (ja) | 1997-11-10 | 1999-05-25 | Toyobo Co Ltd | β1,4−ガラクトース転移酵素の製造法 |
| JPH11253163A (ja) | 1998-03-11 | 1999-09-21 | Toyobo Co Ltd | シアル酸転移酵素の製造法 |
| US6037730A (en) | 1998-11-12 | 2000-03-14 | Eaton Corporation | Degraded electronic throttle operation method and system |
| US6949372B2 (en) * | 1999-03-02 | 2005-09-27 | The Johns Hopkins University | Engineering intracellular sialylation pathways |
| JP2001178453A (ja) | 1999-12-24 | 2001-07-03 | Toyobo Co Ltd | N−アセチルグルコサミニルトランスフェラーゼの製造方法 |
| US7338932B2 (en) * | 2000-05-11 | 2008-03-04 | Glycozym Aps | Methods of modulating functions of polypeptide GalNAc-transferases and of screening test substances to find agents herefor, pharmaceutical compositions comprising such agents and the use of such agents for preparing medicaments |
| EP2042196B1 (de) | 2001-10-10 | 2016-07-13 | ratiopharm GmbH | Neumodellierung und Glykokonjugation von Granulozyten-Kolonie stimulierender Faktor (G-CSF) |
| US7179617B2 (en) * | 2001-10-10 | 2007-02-20 | Neose Technologies, Inc. | Factor IX: remolding and glycoconjugation of Factor IX |
| EP1477028A2 (de) * | 2002-02-21 | 2004-11-17 | BRITISH TELECOMMUNICATIONS public limited company | Videoverarbeitung |
| JP2005520569A (ja) * | 2002-03-23 | 2005-07-14 | リサーチ ディベロップメント ファンデーション | バクテリア内で複数のジスルフィド結合を有する蛋白質の分泌とその利用 |
| WO2003093448A2 (en) * | 2002-05-03 | 2003-11-13 | Neose Technologies, Inc. | Recombinant glycosyltransferase fusion proteins |
| JP2004016116A (ja) | 2002-06-17 | 2004-01-22 | Toyobo Co Ltd | β1,4−N−アセチルガラクトサミニルトランスフェラーゼ活性を有する融合蛋白質及びその製造方法 |
| JP2004016117A (ja) | 2002-06-17 | 2004-01-22 | Toyobo Co Ltd | β1,3−ガラクトシルトランスフェラーゼ活性を有する融合蛋白質及びその製造方法 |
| US7662583B2 (en) * | 2003-05-27 | 2010-02-16 | The University Of York | Bioreactor containing cells expressing glycosyltransferase nucleic acids isolatable from Arabidopsis |
| JP2005046030A (ja) | 2003-07-31 | 2005-02-24 | Toyobo Co Ltd | I分岐形成β1,6−N−アセチルグルコサミニルトランスフェラーゼ活性を有する融合タンパク質及びその製造方法 |
| JP2005046031A (ja) | 2003-07-31 | 2005-02-24 | Toyobo Co Ltd | α1,3−フコシルトランスフェラーゼVI活性を有する融合タンパク質及びその製造方法 |
| NZ548893A (en) * | 2004-02-04 | 2009-09-25 | Biogernerix Ag | Methods of refolding mammalian glycosyltransferases |
| WO2006102652A2 (en) | 2005-03-24 | 2006-09-28 | Neose Technologies, Inc. | Expression of soluble, active eukaryotic glycosyltransferases in prokaryotic organisms |
-
2006
- 2006-03-24 WO PCT/US2006/011065 patent/WO2006102652A2/en not_active Ceased
- 2006-03-24 AT AT06748724T patent/ATE527371T1/de not_active IP Right Cessation
- 2006-03-24 ES ES06748724T patent/ES2374935T3/es not_active Expired - Lifetime
- 2006-03-24 MX MX2007011801A patent/MX2007011801A/es not_active Application Discontinuation
- 2006-03-24 CA CA2602329A patent/CA2602329C/en not_active Expired - Fee Related
- 2006-03-24 US US11/909,599 patent/US20100143969A1/en not_active Abandoned
- 2006-03-24 KR KR1020077024354A patent/KR20080021590A/ko not_active Withdrawn
- 2006-03-24 US US11/388,595 patent/US8137928B2/en not_active Expired - Fee Related
- 2006-03-24 AU AU2006226782A patent/AU2006226782B2/en not_active Ceased
- 2006-03-24 EP EP06748724A patent/EP1863921B1/de not_active Expired - Lifetime
- 2006-03-24 JP JP2008503276A patent/JP5235657B2/ja not_active Expired - Fee Related
-
2011
- 2011-03-28 US US13/073,445 patent/US20110223646A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| MX2007011801A (es) | 2008-02-19 |
| JP5235657B2 (ja) | 2013-07-10 |
| EP1863921B1 (de) | 2011-10-05 |
| AU2006226782B2 (en) | 2011-12-08 |
| WO2006102652A2 (en) | 2006-09-28 |
| US20100143969A1 (en) | 2010-06-10 |
| EP1863921A2 (de) | 2007-12-12 |
| JP2008533992A (ja) | 2008-08-28 |
| US20110223646A1 (en) | 2011-09-15 |
| WO2006102652A3 (en) | 2007-05-24 |
| AU2006226782A1 (en) | 2006-09-28 |
| CA2602329A1 (en) | 2006-09-28 |
| KR20080021590A (ko) | 2008-03-07 |
| US8137928B2 (en) | 2012-03-20 |
| US20060234345A1 (en) | 2006-10-19 |
| CA2602329C (en) | 2016-08-09 |
| EP1863921A4 (de) | 2009-04-08 |
| ES2374935T3 (es) | 2012-02-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |