ATE527346T1 - Neue plastidgezielte nukleinsäure sequenz, neue beta-amylase sequenz, stimulus-abhängiger promoter und deren verwendungen - Google Patents

Neue plastidgezielte nukleinsäure sequenz, neue beta-amylase sequenz, stimulus-abhängiger promoter und deren verwendungen

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Publication number
ATE527346T1
ATE527346T1 AT99940330T AT99940330T ATE527346T1 AT E527346 T1 ATE527346 T1 AT E527346T1 AT 99940330 T AT99940330 T AT 99940330T AT 99940330 T AT99940330 T AT 99940330T AT E527346 T1 ATE527346 T1 AT E527346T1
Authority
AT
Austria
Prior art keywords
beta
amylase
sequence
new
nucleic acid
Prior art date
Application number
AT99940330T
Other languages
English (en)
Inventor
Thomas Anthony Kavanagh
Nga Thi Lao
Original Assignee
Cambridge Advanced Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9817959.1A external-priority patent/GB9817959D0/en
Priority claimed from GBGB9817963.3A external-priority patent/GB9817963D0/en
Priority claimed from GBGB9913014.8A external-priority patent/GB9913014D0/en
Application filed by Cambridge Advanced Tech filed Critical Cambridge Advanced Tech
Application granted granted Critical
Publication of ATE527346T1 publication Critical patent/ATE527346T1/de

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8221—Transit peptides
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2425—Beta-amylase (3.2.1.2)

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  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Saccharide Compounds (AREA)
AT99940330T 1998-08-19 1999-08-13 Neue plastidgezielte nukleinsäure sequenz, neue beta-amylase sequenz, stimulus-abhängiger promoter und deren verwendungen ATE527346T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9817959.1A GB9817959D0 (en) 1998-08-19 1998-08-19 A novel plastid-targeting nucleic acid sequence and a novel B-amylase sequence and uses therefor
GBGB9817963.3A GB9817963D0 (en) 1998-08-19 1998-08-19 A plastid-targeting nucleic acid sequence and uses therefor
GBGB9913014.8A GB9913014D0 (en) 1999-06-05 1999-06-05 A plastid-targeting nucleic acid sequence,a stimulus-responsive promoter,and uses thereof
PCT/GB1999/002697 WO2000011144A2 (en) 1998-08-19 1999-08-13 Plastid-targeting nucleic acid sequence, beta-amylase sequence, a stimulus-responsive promoter and uses thereof

Publications (1)

Publication Number Publication Date
ATE527346T1 true ATE527346T1 (de) 2011-10-15

Family

ID=27269441

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99940330T ATE527346T1 (de) 1998-08-19 1999-08-13 Neue plastidgezielte nukleinsäure sequenz, neue beta-amylase sequenz, stimulus-abhängiger promoter und deren verwendungen

Country Status (18)

Country Link
US (2) US6489540B1 (de)
EP (1) EP1105468B1 (de)
JP (2) JP2002523040A (de)
CN (2) CN1528904B (de)
AT (1) ATE527346T1 (de)
AU (1) AU773492B2 (de)
BR (1) BR9914299A (de)
CA (1) CA2336249C (de)
CZ (1) CZ303574B6 (de)
ES (1) ES2375034T3 (de)
HU (1) HU228696B1 (de)
MX (1) MXPA01001815A (de)
NZ (1) NZ509642A (de)
PL (1) PL205558B1 (de)
SK (1) SK288125B6 (de)
TR (1) TR200100625T2 (de)
WO (1) WO2000011144A2 (de)
ZA (1) ZA200100736B (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3546337B2 (ja) * 1993-12-21 2004-07-28 ゼロックス コーポレイション 計算システム用ユーザ・インタフェース装置及びグラフィック・キーボード使用方法
TR200100625T2 (tr) 1998-08-19 2001-06-21 Advanced Technologies (Cambridge) Limited Nükleik asit serisini hedefleyen yeni plastidlerin kullanımları
MXPA03007469A (es) 2001-02-22 2004-07-30 Pioneer Hi Bred Int Manipulacion de genes sucrosa sintasa para mejorar la calidad del grano y tallo.
JP2004535789A (ja) * 2001-04-25 2004-12-02 マックス−プランク−ゲゼルシャフト ツール フォルデルング デル ヴィッセンシャフテン エー．ファウ． デンプン分解酵素をコードする核酸分子
AR036370A1 (es) * 2001-08-27 2004-09-01 Syngenta Participations Ag Plantas con auto-procesamiento y partes de las mismas
AU2003279371C1 (en) * 2002-11-08 2010-10-14 Bayer Intellectual Property Gmbh Process for reducing the acrylamide content of heat-treated foods
JP2007151435A (ja) * 2005-12-02 2007-06-21 Niigata Univ デンプン集積能力の高い形質転換植物およびその製造方法
CN101910404A (zh) * 2007-11-05 2010-12-08 先正达参股股份有限公司 提高植物淀粉含量的方法
US20120054915A1 (en) * 2009-02-25 2012-03-01 Syngenta Participations Ag Methods for increasing starch content in plant cobs
US10443068B2 (en) 2010-06-25 2019-10-15 Agrivida, Inc. Plants with engineered endogenous genes
WO2011163659A2 (en) 2010-06-25 2011-12-29 Agrivida, Inc. Plants with altered levels of vegetative starch
US9598700B2 (en) 2010-06-25 2017-03-21 Agrivida, Inc. Methods and compositions for processing biomass with elevated levels of starch
WO2015021600A1 (en) * 2013-08-13 2015-02-19 Danisco Us Inc. Beta-amylase and methods of use
CN104232681B (zh) * 2014-09-28 2017-02-15 浙江大学 一种植物表达载体及在制备磷酸化改性水稻淀粉中的应用
CN117481005B (zh) * 2023-09-21 2024-08-02 中国烟草总公司广东省公司 一种坡地烟与山旱稻双优融合的种植方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5034323A (en) 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5391725A (en) * 1989-12-08 1995-02-21 New York University Medical Center Organ-specific plant promoter sequences
US5451513A (en) * 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5349123A (en) * 1990-12-21 1994-09-20 Calgene, Inc. Glycogen biosynthetic enzymes in plants
AU644203B2 (en) 1990-06-18 1993-12-02 Monsanto Company Increased starch content in plants
US5750876A (en) * 1994-07-28 1998-05-12 Monsanto Company Isoamylase gene, compositions containing it, and methods of using isoamylases
GB9522589D0 (en) * 1995-11-03 1996-01-03 Innes John Centre Modified plants and plant products
JP4159114B2 (ja) 1996-09-03 2008-10-01 バイエル バイオサイエンス エヌ．ブイ 改良されたバースター（ｂａｒｓｔａｒ）遺伝子
TR200100625T2 (tr) 1998-08-19 2001-06-21 Advanced Technologies (Cambridge) Limited Nükleik asit serisini hedefleyen yeni plastidlerin kullanımları

Also Published As

Publication number Publication date
MXPA01001815A (es) 2002-06-21
NZ509642A (en) 2003-08-29
PL205558B1 (pl) 2010-05-31
JP4673431B2 (ja) 2011-04-20
HU228696B1 (en) 2013-05-28
CN1323349A (zh) 2001-11-21
AU5432699A (en) 2000-03-14
CN1528904A (zh) 2004-09-15
ES2375034T3 (es) 2012-02-24
CZ2001460A3 (cs) 2001-11-14
CN1528904B (zh) 2010-05-26
PL346704A1 (en) 2002-02-25
HUP0200732A2 (hu) 2002-07-29
CZ303574B6 (cs) 2012-12-19
CN1234868C (zh) 2006-01-04
JP2010131029A (ja) 2010-06-17
AU773492B2 (en) 2004-05-27
BR9914299A (pt) 2001-11-27
CA2336249C (en) 2011-03-08
EP1105468B1 (de) 2011-10-05
US20030074690A1 (en) 2003-04-17
SK2042001A3 (en) 2002-01-07
SK288125B6 (sk) 2013-10-02
WO2000011144A2 (en) 2000-03-02
WO2000011144A3 (en) 2000-09-08
EP1105468A2 (de) 2001-06-13
HUP0200732A3 (en) 2004-10-28
ZA200100736B (en) 2002-06-26
US6489540B1 (en) 2002-12-03
CA2336249A1 (en) 2000-03-02
JP2002523040A (ja) 2002-07-30
TR200100625T2 (tr) 2001-06-21

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