ATE535603T1 - Mutante reverse transkriptase und verwendungsverfahren - Google Patents

Mutante reverse transkriptase und verwendungsverfahren

Info

Publication number
ATE535603T1
ATE535603T1 AT06801382T AT06801382T ATE535603T1 AT E535603 T1 ATE535603 T1 AT E535603T1 AT 06801382 T AT06801382 T AT 06801382T AT 06801382 T AT06801382 T AT 06801382T AT E535603 T1 ATE535603 T1 AT E535603T1
Authority
AT
Austria
Prior art keywords
methods
transcriptasis
mutant reverse
reverse transcriptase
mmlv reverse
Prior art date
Application number
AT06801382T
Other languages
English (en)
Inventor
Holly Hogrefe
Bahram Arezi
Weimei Xing
Original Assignee
Stratagene California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stratagene California filed Critical Stratagene California
Application granted granted Critical
Publication of ATE535603T1 publication Critical patent/ATE535603T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT06801382T 2005-08-10 2006-08-10 Mutante reverse transkriptase und verwendungsverfahren ATE535603T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70701905P 2005-08-10 2005-08-10
PCT/US2006/031567 WO2007022045A2 (en) 2005-08-10 2006-08-10 Mutant reverse transcriptase and methods of use

Publications (1)

Publication Number Publication Date
ATE535603T1 true ATE535603T1 (de) 2011-12-15

Family

ID=37758252

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06801382T ATE535603T1 (de) 2005-08-10 2006-08-10 Mutante reverse transkriptase und verwendungsverfahren

Country Status (5)

Country Link
EP (1) EP1931772B1 (de)
JP (1) JP5203200B2 (de)
AT (1) ATE535603T1 (de)
CA (1) CA2617790A1 (de)
WO (1) WO2007022045A2 (de)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0806562D0 (en) * 2008-04-10 2008-05-14 Fermentas Uab Production of nucleic acid
EP2604688B1 (de) * 2010-08-13 2018-01-10 Kyoto University Variante reverse transkriptase
KR101818126B1 (ko) 2011-02-09 2018-01-15 (주)바이오니아 열안정성이 증가된 역전사효소
ES2525135B1 (es) * 2013-05-17 2015-09-29 Consejo Superior De Investigaciones Científicas (Csic) Retrotranscriptasas del vih tipo 1 grupo o, activas a temperaturas elevadas
US9663770B2 (en) 2014-01-22 2017-05-30 Life Technologies Corporation Reverse transcriptases for use in high temperature nucleic acid synthesis
CN106414704B (zh) 2014-03-14 2020-11-03 生命技术公司 用于核酸扩增和检测的集成系统
US11046940B2 (en) 2016-04-12 2021-06-29 Solis Biodyne Ou Synthetic reverse transcriptases and uses thereof
JP7061078B2 (ja) * 2016-12-14 2022-04-27 タカラバイオ株式会社 耐熱性の逆転写酵素変異体
EP4375376A3 (de) 2017-04-26 2024-08-14 10X Genomics, Inc. Mmlv-reverse-transkriptase-varianten
CN113785053B (zh) * 2018-12-26 2024-07-12 深圳华大生命科学研究院 酶活性提高的逆转录酶及其应用
CN112795550B (zh) * 2019-11-13 2024-05-31 广州达安基因股份有限公司 耐高温逆转录酶突变体
CN112795551B (zh) * 2019-11-13 2024-01-30 广州达安基因股份有限公司 一种耐高温逆转录酶突变体及其应用
EP4073241A2 (de) 2019-12-11 2022-10-19 10X Genomics, Inc. Varianten der reversen transcriptase
EP4114937A4 (de) * 2020-03-04 2024-09-04 Flagship Pioneering Innovations VI, LLC Verfahren und zusammensetzungen zur modulierung eines genoms
US12559734B2 (en) 2020-07-20 2026-02-24 Integrated Dna Technologies, Inc. Reverse transcriptase mutants with increased activity and thermostability
WO2022020371A2 (en) 2020-07-20 2022-01-27 Integrated Dna Technologies, Inc. Reverse transcriptase mutants with increased activity and thermostability
JP2022165387A (ja) * 2021-04-19 2022-10-31 東ソー株式会社 トリ骨髄芽細胞腫ウイルス逆転写酵素発現ベクターおよび当該ベクターを用いた前記酵素の製造法
WO2022265965A1 (en) * 2021-06-14 2022-12-22 10X Genomics, Inc. Reverse transcriptase variants for improved performance
WO2023114473A2 (en) * 2021-12-16 2023-06-22 10X Genomics, Inc. Recombinant reverse transcriptase variants for improved performance
CN114317485B (zh) * 2021-12-30 2023-04-11 南京巨匠生物科技有限公司 重组鼠白血病病毒逆转录酶突变体、制备方法及应用
WO2023164726A2 (en) * 2022-02-28 2023-08-31 Integrated Dna Technologies, Inc. C-terminal peptide extensions with increased activity
US11639499B1 (en) * 2022-03-08 2023-05-02 Watchmaker Genomics, Inc. Reverse transcriptase variants
CN115261352B (zh) * 2022-06-08 2024-03-29 厦门通灵生物医药科技有限公司 一种耐高温的突变型逆转录酶及其制备方法
CN116814583A (zh) * 2022-06-29 2023-09-29 武汉爱博泰克生物科技有限公司 一种Taq DNA聚合酶突变体及其应用
WO2025118254A1 (zh) * 2023-12-07 2025-06-12 深圳华大生命科学研究院 逆转录酶及其应用
WO2026003215A1 (fr) 2024-06-27 2026-01-02 Commissariat A L'energie Atomique Et Aux Energies Alternatives Bases modifiées chimiquement avec un groupement clivable et méthodes de sélection d'acides nucléiques fonctionnels les comprenant

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4193079B2 (ja) * 1998-11-10 2008-12-10 東洋紡績株式会社 変異型逆転写酵素
WO2001068895A1 (en) * 2000-03-15 2001-09-20 Invitrogen Corporation High fidelity reverse transcriptases and uses thereof
CN1430670A (zh) * 2000-05-26 2003-07-16 茵维特罗根公司 热稳定逆转录酶及其用途
EP2325303B1 (de) * 2002-09-13 2013-11-06 Life Technologies Corporation Wärmestabile reverse Transkriptasen und Verwendungen davon
WO2005071072A1 (en) * 2004-01-13 2005-08-04 The Institute Of Biophysics Chinese Academy Of Sciences The recombinant murine leukemia virus reverse transcriptase, the gene encoding it and the method for expressing it

Also Published As

Publication number Publication date
JP2009504162A (ja) 2009-02-05
EP1931772A2 (de) 2008-06-18
EP1931772A4 (de) 2009-12-30
JP5203200B2 (ja) 2013-06-05
CA2617790A1 (en) 2007-02-22
EP1931772B1 (de) 2011-11-30
WO2007022045A2 (en) 2007-02-22
WO2007022045A3 (en) 2009-05-22

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