ATE540975T1 - Verbesserte faktor-viii-polypeptid-titer in zellkulturen - Google Patents

Verbesserte faktor-viii-polypeptid-titer in zellkulturen

Info

Publication number
ATE540975T1
ATE540975T1 AT08749930T AT08749930T ATE540975T1 AT E540975 T1 ATE540975 T1 AT E540975T1 AT 08749930 T AT08749930 T AT 08749930T AT 08749930 T AT08749930 T AT 08749930T AT E540975 T1 ATE540975 T1 AT E540975T1
Authority
AT
Austria
Prior art keywords
factor viii
viii polypeptide
cell cultures
improved factor
polypeptide titer
Prior art date
Application number
AT08749930T
Other languages
English (en)
Inventor
Laust Johnsen
Ida Hilden
Gert Bolt
Thomas Steenstrup
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38519867&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE540975(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE540975T1 publication Critical patent/ATE540975T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT08749930T 2007-05-04 2008-04-30 Verbesserte faktor-viii-polypeptid-titer in zellkulturen ATE540975T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07107477A EP1988101A1 (de) 2007-05-04 2007-05-04 Verbesserung von Faktor-VIII-Polypeptid-Titern in Zellkulturen
PCT/EP2008/055349 WO2008135501A1 (en) 2007-05-04 2008-04-30 Improvement of factor viii polypeptide titers in cell cultures

Publications (1)

Publication Number Publication Date
ATE540975T1 true ATE540975T1 (de) 2012-01-15

Family

ID=38519867

Family Applications (1)

Application Number Title Priority Date Filing Date
AT08749930T ATE540975T1 (de) 2007-05-04 2008-04-30 Verbesserte faktor-viii-polypeptid-titer in zellkulturen

Country Status (14)

Country Link
US (1) US9982033B2 (de)
EP (2) EP1988101A1 (de)
JP (1) JP5506664B2 (de)
KR (1) KR101594805B1 (de)
CN (1) CN101675073B (de)
AT (1) ATE540975T1 (de)
AU (1) AU2008248627B2 (de)
BR (1) BRPI0811177A2 (de)
CA (1) CA2684951A1 (de)
ES (1) ES2379846T3 (de)
MX (1) MX2009011805A (de)
PL (1) PL2144929T3 (de)
RU (1) RU2477318C2 (de)
WO (1) WO2008135501A1 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
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MX353340B (es) 2006-09-13 2018-01-09 Abbvie Inc Mejoras de cultivos celulares.
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
SG195577A1 (en) 2008-10-20 2013-12-30 Abbott Lab Viral inactivation during purification of antibodies
TW201024318A (en) 2008-10-20 2010-07-01 Abbott Lab Isolation and purification of antibodies using protein A affinity chromatography
WO2012045769A1 (en) 2010-10-05 2012-04-12 Novo Nordisk Health Care Ag Process for protein production
US9062106B2 (en) 2011-04-27 2015-06-23 Abbvie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
EP2782929A1 (de) * 2011-11-21 2014-10-01 Novo Nordisk A/S Verfahren zur herstellung von faktor viii
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
KR101395736B1 (ko) * 2012-07-10 2014-05-16 아주대학교산학협력단 인간 혈액응고인자 ⅷ 도메인 특이적 항체 및 이의 제조방법
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
KR20150043523A (ko) 2012-09-02 2015-04-22 애브비 인코포레이티드 단백질 불균일성의 제어 방법
SG11201507230PA (en) 2013-03-12 2015-10-29 Abbvie Inc Human antibodies that bind human tnf-alpha and methods of preparing the same
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
WO2014151878A2 (en) 2013-03-14 2014-09-25 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
EP3052640A2 (de) 2013-10-04 2016-08-10 AbbVie Inc. Verwendung von metallionen zur modulation von proteinglykosylierungsprofilen rekombinanter proteine
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
WO2015158696A1 (en) * 2014-04-16 2015-10-22 Cmc Biologics A/S A high cell density fill and draw fermentation process
JP6516829B2 (ja) 2014-08-04 2019-05-22 シーエスエル、リミテッド 第ｖｉｉｉ因子製剤
CA2978374A1 (en) 2015-03-06 2016-09-15 Csl Behring Recombinant Facility Ag Modified von willebrand factor having improved half-life
KR20220029733A (ko) 2019-07-04 2022-03-08 체에스엘 베링 렝나우 아게 응고 인자 viii의 시험관내 안정성을 증가시키기 위한 절단된 폰 빌레브란트 인자 (vwf)
EP4058049A1 (de) 2019-11-11 2022-09-21 CSL Behring Lengnau AG Polypeptide zur induktion der toleranz gegen faktor viii
KR20250110226A (ko) 2022-11-23 2025-07-18 에프. 호프만-라 로슈 아게 재조합 단백질 발현을 증가시키는 방법

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5672502A (en) 1985-06-28 1997-09-30 Celltech Therapeutics Limited Animal cell culture
JP2525022B2 (ja) 1986-01-03 1996-08-14 ジェネティックス・インスチチュ−ト・インコ−ポレ−テッド ▲ＶＩＩＩ▼：ｃ因子型タンパク質の改良生産方法
US5250421A (en) * 1986-01-03 1993-10-05 Genetics Institute, Inc. Method for producing factor VIII:C-type proteins
EP0254076B1 (de) * 1986-07-11 1991-05-08 Miles Inc. Herstellung von rekombinantem Protein
CA1331157C (en) 1987-04-06 1994-08-02 Randal J. Kaufman Method for producing factor viii:c-type proteins
WO1990002175A1 (en) 1988-08-16 1990-03-08 Novo Nordisk A/S A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors
US5679549A (en) * 1995-05-04 1997-10-21 Bayer Corporation Production of recombinant factor VIII in the presence of liposome-like substances of mixed composition
US5952198A (en) 1995-05-04 1999-09-14 Bayer Corporation Production of recombinant Factor VIII in the presence of liposome-like substances of mixed composition
US5851800A (en) 1996-05-14 1998-12-22 Pharmacia & Upjohn Ab Process for producing a protein
US6458563B1 (en) 1996-06-26 2002-10-01 Emory University Modified factor VIII
US6338964B1 (en) 1999-05-07 2002-01-15 Bayer Corporation Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration
AT409379B (de) 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
EP1359935A1 (de) * 2001-02-05 2003-11-12 Novo Nordisk Health Care AG Kombinierte verwendung von faktor vii polypeptiden und factor ix polypeptiden
US7351688B2 (en) * 2003-02-05 2008-04-01 The Research Foundation Of State University Of New York Compositions and methods for less immunogenic protein formulations
US20040229793A1 (en) 2003-02-05 2004-11-18 Balasubramanian Sathyamangalam V. Compositions and methods for less immunogenic protein formulations
EP1707634A1 (de) * 2005-03-29 2006-10-04 Octapharma AG Verfahren zur Isolierung von rekombinant hergestellten Proteinen

Also Published As

Publication number Publication date
US20100120094A1 (en) 2010-05-13
EP2144929B1 (de) 2012-01-11
JP5506664B2 (ja) 2014-05-28
KR20100016430A (ko) 2010-02-12
RU2477318C2 (ru) 2013-03-10
PL2144929T3 (pl) 2012-06-29
EP2144929A1 (de) 2010-01-20
CA2684951A1 (en) 2008-11-13
MX2009011805A (es) 2009-11-13
CN101675073A (zh) 2010-03-17
WO2008135501A1 (en) 2008-11-13
EP1988101A1 (de) 2008-11-05
KR101594805B1 (ko) 2016-02-17
AU2008248627B2 (en) 2013-06-06
BRPI0811177A2 (pt) 2014-10-29
US9982033B2 (en) 2018-05-29
ES2379846T3 (es) 2012-05-04
JP2010525829A (ja) 2010-07-29
CN101675073B (zh) 2013-12-04
AU2008248627A1 (en) 2008-11-13
RU2009143635A (ru) 2011-06-10

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