ATE548466T1 - Verfahren und zusammensetzungen zur verbesserung der genauigkeit bei einer nukleinsäuresynthesereaktion - Google Patents

Verfahren und zusammensetzungen zur verbesserung der genauigkeit bei einer nukleinsäuresynthesereaktion

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Publication number
ATE548466T1
ATE548466T1 AT06734692T AT06734692T ATE548466T1 AT E548466 T1 ATE548466 T1 AT E548466T1 AT 06734692 T AT06734692 T AT 06734692T AT 06734692 T AT06734692 T AT 06734692T AT E548466 T1 ATE548466 T1 AT E548466T1
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Prior art keywords
compositions
improving
synthesis reaction
accuracy
methods
Prior art date
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AT06734692T
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English (en)
Inventor
Philip Buzby
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Helicos Biosciences Corp
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Publication of ATE548466T1 publication Critical patent/ATE548466T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT06734692T 2005-01-28 2006-01-30 Verfahren und zusammensetzungen zur verbesserung der genauigkeit bei einer nukleinsäuresynthesereaktion ATE548466T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/046,448 US7482120B2 (en) 2005-01-28 2005-01-28 Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
PCT/US2006/004638 WO2006081583A2 (en) 2005-01-28 2006-01-30 Methods and compositions for improving fidelity in a nucleic acid synthesis reaction

Publications (1)

Publication Number Publication Date
ATE548466T1 true ATE548466T1 (de) 2012-03-15

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AT06734692T ATE548466T1 (de) 2005-01-28 2006-01-30 Verfahren und zusammensetzungen zur verbesserung der genauigkeit bei einer nukleinsäuresynthesereaktion

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US (1) US7482120B2 (de)
EP (1) EP1848829B1 (de)
AT (1) ATE548466T1 (de)
CA (1) CA2599477A1 (de)
WO (1) WO2006081583A2 (de)

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EP1848829A2 (de) 2007-10-31
US20060172313A1 (en) 2006-08-03
WO2006081583A2 (en) 2006-08-03
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US7482120B2 (en) 2009-01-27
CA2599477A1 (en) 2006-08-03

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