ATE553218T1 - Differentielle enzymatische fragmetierung - Google Patents

Differentielle enzymatische fragmetierung

Info

Publication number
ATE553218T1
ATE553218T1 AT04796072T AT04796072T ATE553218T1 AT E553218 T1 ATE553218 T1 AT E553218T1 AT 04796072 T AT04796072 T AT 04796072T AT 04796072 T AT04796072 T AT 04796072T AT E553218 T1 ATE553218 T1 AT E553218T1
Authority
AT
Austria
Prior art keywords
fragmeting
differential enzymatic
enzymatic
differential
locus
Prior art date
Application number
AT04796072T
Other languages
English (en)
Inventor
Jeffrey Jeddeloh
Nathan Lakey
Original Assignee
Orion Genomics Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orion Genomics Llc filed Critical Orion Genomics Llc
Application granted granted Critical
Publication of ATE553218T1 publication Critical patent/ATE553218T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT04796072T 2003-10-21 2004-10-21 Differentielle enzymatische fragmetierung ATE553218T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US51342603P 2003-10-21 2003-10-21
US56156304P 2004-04-12 2004-04-12
US56172104P 2004-04-12 2004-04-12
PCT/US2004/035012 WO2005042704A2 (en) 2003-10-21 2004-10-21 Differential enzymatic fragmentation

Publications (1)

Publication Number Publication Date
ATE553218T1 true ATE553218T1 (de) 2012-04-15

Family

ID=34527938

Family Applications (2)

Application Number Title Priority Date Filing Date
AT04796212T ATE550438T1 (de) 2003-10-21 2004-10-21 Verfahren zur quantitativen bestimmung der methylierungsdichte in einem dna-locus
AT04796072T ATE553218T1 (de) 2003-10-21 2004-10-21 Differentielle enzymatische fragmetierung

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT04796212T ATE550438T1 (de) 2003-10-21 2004-10-21 Verfahren zur quantitativen bestimmung der methylierungsdichte in einem dna-locus

Country Status (8)

Country Link
US (4) US7910296B2 (de)
EP (4) EP1689887B1 (de)
JP (2) JP4887154B2 (de)
AT (2) ATE550438T1 (de)
AU (2) AU2004286235B2 (de)
CA (2) CA2542542C (de)
ES (2) ES2383970T3 (de)
WO (2) WO2005042704A2 (de)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003298733B2 (en) * 2002-11-27 2009-06-18 Agena Bioscience, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
EP1618216A2 (de) * 2003-04-25 2006-01-25 Sequenom, Inc. Auf fragmentierung beruhende verfahren und systeme zur de-novo-sequenzierung
US9394565B2 (en) 2003-09-05 2016-07-19 Agena Bioscience, Inc. Allele-specific sequence variation analysis
WO2005042704A2 (en) 2003-10-21 2005-05-12 Orion Genomics Llc Differential enzymatic fragmentation
WO2005085477A1 (en) * 2004-03-02 2005-09-15 Orion Genomics Llc Differential enzymatic fragmentation by whole genome amplification
US7608394B2 (en) 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
AU2005230936B2 (en) 2004-03-26 2010-08-05 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
DE602004030430D1 (de) * 2004-06-23 2011-01-20 Epigenomics Ag Verfahren zur Quantifizierung von methylierter DNS
WO2006031745A2 (en) * 2004-09-10 2006-03-23 Sequenom, Inc. Methods for long-range sequence analysis of nucleic acids
US7820385B2 (en) * 2006-03-22 2010-10-26 The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention Method for retaining methylation pattern in globally amplified DNA
US7754428B2 (en) 2006-05-03 2010-07-13 The Chinese University Of Hong Kong Fetal methylation markers
WO2008008501A2 (en) * 2006-07-14 2008-01-17 University Of Georgia Research Foundation, Inc. Reverse restriction fragment length polymorphism assay and uses thereof
CN101153336B (zh) 2006-09-27 2011-09-07 香港中文大学 检测dna甲基化程度的方法和试剂盒
EP2087137B1 (de) * 2006-10-18 2011-11-30 Epigenomics AG Molekül zur bereitstellung eines standards zur quantitativen analyse des methylierungsstatus einer nukleinsäure
JP2008136404A (ja) * 2006-11-30 2008-06-19 Sysmex Corp Ｄｎａメチル化検出における非メチル化シトシン変換処理後のｄｎａ量の確認方法
WO2008091913A2 (en) 2007-01-23 2008-07-31 Sequenom, Inc. Method for accurate assessment of dna quality after bisulfite treatment
JP2010524454A (ja) * 2007-04-19 2010-07-22 モレキュラー ディテクション インコーポレーテッド 抗生物質耐性細菌の検出および分析のための方法、組成物、およびキット
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
US8709726B2 (en) * 2008-03-11 2014-04-29 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US20090253121A1 (en) * 2008-04-04 2009-10-08 Micah Halpern Method for amt-rflp dna fingerprinting
BRPI0913578A2 (pt) 2008-05-14 2017-06-06 Dermtech Int diagnose de melanoma e lentigo solar por análise de ácido nucléico
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
WO2010104737A1 (en) * 2009-03-09 2010-09-16 New England Biolabs, Inc. Engineering a novel methylation-specific restriction endonuclease
WO2011001274A2 (en) * 2009-07-02 2011-01-06 Nucleix Methods for distinguishing between natural and artificial dna samples
JP2013511991A (ja) * 2009-11-25 2013-04-11 クアンタライフ， インコーポレイテッド 遺伝子材料を検出する方法および組成物
US9783850B2 (en) * 2010-02-19 2017-10-10 Nucleix Identification of source of DNA samples
CA2783739C (en) * 2009-12-11 2019-12-31 Nucleix Categorization of dna samples
US9752187B2 (en) 2009-12-11 2017-09-05 Nucleix Categorization of DNA samples
US9926593B2 (en) 2009-12-22 2018-03-27 Sequenom, Inc. Processes and kits for identifying aneuploidy
GB201003036D0 (en) 2010-02-23 2010-04-07 Fermentas Uab Restriction endonucleases and their applications
EP2542698B1 (de) * 2010-03-03 2015-04-22 Zymo Research Corporation Erkennung einer dna-methylierung
WO2011112845A2 (en) * 2010-03-11 2011-09-15 Board Of Regents, The University Of Texas System Methods and compositions related to a multi-methylation assay to predict patient outcome
JP2013524805A (ja) * 2010-04-20 2013-06-20 ニュークレイックス Ｄｎａ試料のメチル化プロファイリング
US20130316339A1 (en) * 2010-09-01 2013-11-28 Orion Genomics Llc Detection of nucleic acid sequences adjacent to repeated sequences
CN103562407A (zh) 2011-02-09 2014-02-05 伯乐生命医学产品有限公司 核酸的分析
US12097495B2 (en) * 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
CA2834218C (en) 2011-04-29 2021-02-16 Sequenom, Inc. Quantification of a minority nucleic acid species using inhibitory oligonucleotides
WO2013123481A1 (en) 2012-02-16 2013-08-22 Cornell University Methods and kit for characterizing the modified base status of a transcriptome
EP2820129A1 (de) 2012-03-02 2015-01-07 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
CA3083314C (en) 2012-05-11 2023-01-31 Clinical Genomics Pty Ltd Diagnostic gene marker panel for colorectal cancer
US10941449B2 (en) 2012-05-18 2021-03-09 Clinical Genomics Pty. Ltd. Method of screening for colorectal cancer
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2014011928A1 (en) 2012-07-13 2014-01-16 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US10865446B2 (en) 2012-11-02 2020-12-15 The Johns Hopkins University DNA methylation biomarkers of post-partum depression risk
EP2971100A1 (de) 2013-03-13 2016-01-20 Sequenom, Inc. Primer zur analyse der dna-methylierung
ES2746237T3 (es) 2013-03-14 2020-03-05 Univ Cornell Método para la cuantificación relativa de cambios en la metilación del ADN, usando reacciones combinadas de nucleasa, ligamiento, y polimerasa
US10280464B2 (en) 2013-07-11 2019-05-07 The Johns Hopkins University DNA methylation and genotype specific biomarker of suicide attempt and/or suicide ideation
ES2685893T3 (es) * 2013-07-30 2018-10-15 Bgi Genomics Co., Limited Método para determinar la composición de ácidos nucleicos de una mezcla de ácidos nucleicos
WO2015047910A1 (en) * 2013-09-30 2015-04-02 Us Biomarkers, Inc. Biomarkers for detection of colorectal cancer
US11578373B2 (en) 2019-03-26 2023-02-14 Dermtech, Inc. Gene classifiers and uses thereof in skin cancers
EP3736344A1 (de) 2014-03-13 2020-11-11 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
MY193347A (en) 2014-05-02 2022-10-06 Malaysian Palm Oil Board Mantle phenotype detection in palm
CN104268441B (zh) * 2014-09-23 2017-04-12 中国科学院海洋研究所 一种基于生物信息学获得双dna限制性内切酶组合的方法
US11072828B2 (en) 2014-10-06 2021-07-27 The Johns Hopkins University DNA methylation and genotype specific biomarker for predicting post-traumatic stress disorder
WO2016061624A1 (en) * 2014-10-20 2016-04-28 Commonwealth Scientific And Industrial Research Organisation Genome methylation analysis
US9984201B2 (en) 2015-01-18 2018-05-29 Youhealth Biotech, Limited Method and system for determining cancer status
WO2016138105A2 (en) 2015-02-24 2016-09-01 Zymo Research Corporation Assays to determine dna methylation and dna methylation markers of cancer
US9476100B1 (en) 2015-07-06 2016-10-25 Nucleix Ltd. Methods for diagnosing bladder cancer
US10913986B2 (en) 2016-02-01 2021-02-09 The Board Of Regents Of The University Of Nebraska Method of identifying important methylome features and use thereof
EP3464642A4 (de) 2016-05-31 2020-02-19 The Regents of the University of California Verfahren zur bewertung, überwachung und modulation des alterungsprozesses
US12359257B2 (en) 2016-07-06 2025-07-15 Helio Health Inc. Liver cancer methylation markers and uses thereof
US10093986B2 (en) 2016-07-06 2018-10-09 Youhealth Biotech, Limited Leukemia methylation markers and uses thereof
WO2018009707A1 (en) 2016-07-06 2018-01-11 Youhealth Biotech, Limited Solid tumor methylation markers and uses thereof
WO2018009703A1 (en) 2016-07-06 2018-01-11 Youhealth Biotech, Limited Breast and ovarian cancer methylation markers and uses thereof
US12351876B2 (en) 2016-07-06 2025-07-08 Helio Health, Inc. Colon cancer methylation markers and uses thereof
CN106701939A (zh) * 2016-12-22 2017-05-24 中国热带农业科学院热带生物技术研究所 一种胞嘧啶甲基化挖掘的方法
US10513739B2 (en) 2017-03-02 2019-12-24 Youhealth Oncotech, Limited Methylation markers for diagnosing hepatocellular carcinoma and lung cancer
CA3060553A1 (en) * 2017-04-19 2018-10-25 Singlera Genomics, Inc. Compositions and methods for detection of genomic variance and dna methylation status
JP7220200B2 (ja) 2017-04-19 2023-02-09 シングレラ ジェノミクス， インコーポレイテッド ライブラリー構築および配列解析のための組成物および方法
US11433074B2 (en) 2017-06-22 2022-09-06 Triact Therapeutics, Inc. Methods of treating glioblastoma
EP3687501A4 (de) 2017-09-29 2021-06-23 Triact Therapeutics, Inc. Iniparib-formulierungen und verwendungen davon
US11976332B2 (en) 2018-02-14 2024-05-07 Dermtech, Inc. Gene classifiers and uses thereof in non-melanoma skin cancers
US11959077B2 (en) 2018-05-21 2024-04-16 Battelle Memorial Institute Methods and control compositions for sequencing
US12404540B2 (en) 2018-10-17 2025-09-02 The University Of Queensland Epigenetic biomarker and uses therefor
JP7657149B2 (ja) * 2018-10-31 2025-04-04 ガーダント ヘルス， インコーポレイテッド エピジェネティック区画アッセイを較正するための方法、組成物およびシステム
IL265451B (en) 2019-03-18 2020-01-30 Frumkin Dan Methods and systems for the detection of methylation changes in DNA samples
US20220325316A1 (en) * 2019-08-09 2022-10-13 Battelle Memorial Institute Compositions and methods for detecting methylated dna
US11427874B1 (en) 2019-08-26 2022-08-30 Epi One Inc. Methods and systems for detection of prostate cancer by DNA methylation analysis
CN115398548A (zh) * 2020-02-06 2022-11-25 昂科霍斯特公司 疗法响应的机器学习预测
JP2023544721A (ja) * 2020-09-30 2023-10-25 ガーダント ヘルス， インコーポレイテッド 分配およびメチル化依存性ヌクレアーゼを使用してｄｎａを解析するための組成物および方法
IL302988A (en) * 2020-11-19 2023-07-01 Nucleix Ltd Detection of methylation changes in DNA samples using restriction enzymes and high-throughput sequencing
KR20220096382A (ko) * 2020-12-31 2022-07-07 주식회사 시선바이오머티리얼스 비메틸화 특이적 프로브를 이용한 유전자의 메틸화 검출 방법 및 검출 키트
CN113981046A (zh) * 2021-11-05 2022-01-28 朱运峰 一种基于定量pcr技术dna甲基化检测方法及其试剂盒
WO2023175434A1 (en) * 2022-03-15 2023-09-21 Diagenode S.A. Detection of methylation status of a dna sample
US20260078447A1 (en) 2022-08-22 2026-03-19 University Of Virginia Patent Foundation Dna methylation biomarkers of premenstrual dysphoric disorder and perimenopausal depression
WO2024059649A1 (en) 2022-09-13 2024-03-21 The Saban Research Institute; Children's Hospital Los Angeles Dna methylation signature of retinoblastoma
GB202308414D0 (en) * 2023-06-06 2023-07-19 Nucleix Ltd Markers
CN116926194A (zh) * 2023-06-29 2023-10-24 纳昂达(南京)生物科技有限公司 一种检测dna样本甲基化状态的方法

Family Cites Families (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4376110A (en) 1980-08-04 1983-03-08 Hybritech, Incorporated Immunometric assays using monoclonal antibodies
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
US4517288A (en) 1981-01-23 1985-05-14 American Hospital Supply Corp. Solid phase system for ligand assay
US4732847A (en) 1981-06-09 1988-03-22 University Of Hawaii Monoclonal antibodies for DNA-RNA hybrid complexes and their uses
ZA853756B (en) 1984-06-01 1986-01-29 Miles Lab Nucleic acid hybridization assay employing immobilized rna probes
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
CA1291031C (en) 1985-12-23 1991-10-22 Nikolaas C.J. De Jaeger Method for the detection of specific binding agents and their correspondingbindable substances
DE3803275A1 (de) 1988-02-04 1989-08-17 Dornier Medizintechnik Piezoelektrische stosswellenquelle
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US6033854A (en) 1991-12-16 2000-03-07 Biotronics Corporation Quantitative PCR using blocking oligonucleotides
US5405760A (en) * 1992-04-30 1995-04-11 New England Biolabs, Inc. Process for producing recombinant McrBC endonuclease and cleavage of methylated DNA
WO1995006137A1 (en) 1993-08-27 1995-03-02 Australian Red Cross Society Detection of genes
US5552277A (en) 1994-07-19 1996-09-03 The Johns Hopkins University School Of Medicine Genetic diagnosis of prostate cancer
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US5871917A (en) 1996-05-31 1999-02-16 North Shore University Hospital Research Corp. Identification of differentially methylated and mutated nucleic acids
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6017704A (en) 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5912147A (en) * 1996-10-22 1999-06-15 Health Research, Inc. Rapid means of quantitating genomic instability
US6686151B1 (en) 1998-02-06 2004-02-03 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US7700324B1 (en) 1998-11-03 2010-04-20 The Johns Hopkins University School Of Medicine Methylated CpG island amplification (MCA)
AU2965500A (en) * 1999-01-15 2000-08-01 Gene Logic, Inc. Immobilized nucleic acid hybridization reagent and method
GB9901072D0 (en) 1999-01-19 1999-03-10 Imp Cancer Res Tech Methods for detecting changes to a macromolecular component of a cell
US6605432B1 (en) 1999-02-05 2003-08-12 Curators Of The University Of Missouri High-throughput methods for detecting DNA methylation
US20010046669A1 (en) 1999-02-24 2001-11-29 Mccobmie William R. Genetically filtered shotgun sequencing of complex eukaryotic genomes
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US6180349B1 (en) 1999-05-18 2001-01-30 The Regents Of The University Of California Quantitative PCR method to enumerate DNA copy number
CA2383264A1 (en) 1999-08-13 2001-02-22 Yale University Binary encoded sequence tags
WO2001068807A2 (en) 2000-03-16 2001-09-20 Fred Hutchinson Cancer Research Center Identification of in vivo dna binding loci of chromatin proteins using a tethered nucleotide modification enzyme
US20040033490A1 (en) 2000-03-31 2004-02-19 Laird Peter W. Epigenetic sequences for esophageal adenocarcinoma
WO2001083751A2 (en) * 2000-04-28 2001-11-08 Sangamo Biosciences, Inc. Methods for binding an exogenous molecule to cellular chromatin
US7439016B1 (en) 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
CA2412609C (en) 2000-06-23 2010-09-07 The University Of Chicago Methods for isolating centromere dna
US7300751B2 (en) 2000-06-30 2007-11-27 Syngenta Participations Ag Method for identification of genetic markers
US6498013B1 (en) 2000-07-28 2002-12-24 The Johns Hopkins University Serial analysis of transcript expression using MmeI and long tags
CA2421102A1 (en) 2000-08-25 2002-03-07 Lovelace Respiratory Research Institute Nested methylation-specific polymerase chain reaction cancer detection method
IL154663A0 (en) * 2000-09-01 2003-09-17 Epigenomics Ag Method for determining the degree of methylation of defined cytosines in genomic dna in the sequence context 5'-cpg-3'
JP2004535757A (ja) * 2000-11-08 2004-12-02 ユニバーシティ・オブ・サザン・カリフォルニア ヘミメチル化の検出及び定量のための新規アッセイ法
US6756200B2 (en) 2001-01-26 2004-06-29 The Johns Hopkins University School Of Medicine Aberrantly methylated genes as markers of breast malignancy
US6777187B2 (en) 2001-05-02 2004-08-17 Rubicon Genomics, Inc. Genome walking by selective amplification of nick-translate DNA library and amplification from complex mixtures of templates
DE10128508A1 (de) 2001-06-14 2003-02-06 Epigenomics Ag Verfahren und Nukleinsäuren für die Differenzierung von Prostata-Tumoren
AU2002312588A1 (en) 2001-06-29 2003-03-03 Rubicon Genomics Inc. Methods of using nick translate libraries for snp analysis
WO2003023065A1 (en) 2001-09-06 2003-03-20 Syngenta Participations Ag Dna methylation patterns
EP1438428A4 (de) 2001-09-14 2005-11-16 Univ Queensland Nachweis der methylierung von dna
JP2006500901A (ja) * 2001-09-26 2006-01-12 エピジェンクス ファーマスーティカルズ、 インコーポレイテッド Ｄｎａメチル化変化のアッセイ
WO2003035860A1 (en) 2001-10-24 2003-05-01 The Trustees Of Columbia University In The City Of New York A method for gene identification based on differential dna methylation
DE10154318A1 (de) 2001-10-26 2003-05-15 Epigenomics Ag Verfahren zur Analyse genomischer Methylierungsmuster
US7655791B2 (en) 2001-11-13 2010-02-02 Rubicon Genomics, Inc. DNA amplification and sequencing using DNA molecules generated by random fragmentation
US6960436B2 (en) 2002-02-06 2005-11-01 Epigenomics Ag Quantitative methylation detection in DNA samples
US20040038254A1 (en) 2002-03-08 2004-02-26 Risa Peoples Compositions and methods for detecting nucleic acid methylation
DE60310697D1 (de) 2002-03-15 2007-02-08 Epigenomics Ag Entdeckungs- und diagnoseverfahren mit 5-methylcytosin-dna-glycosylase
US20030175828A1 (en) 2002-03-15 2003-09-18 Lazar James G. Signal amplification by Hybrid Capture
EP2339025B1 (de) 2002-06-26 2013-10-09 Cold Spring Harbor Laboratory Verfahren zur Bestimmung des Methylierungsprofils
AU2003279101A1 (en) 2002-10-02 2004-04-23 Northwestern University Methylation profile of cancer
EP1606417A2 (de) 2003-03-07 2005-12-21 Rubicon Genomics Inc. In-vitro-dna-immortalisierung und gesamtgenomamplifikation unter verwendung von aus willkürlich fragmentierter dna erzeugten bibliotheken
WO2005042704A2 (en) 2003-10-21 2005-05-12 Orion Genomics Llc Differential enzymatic fragmentation
WO2005085477A1 (en) 2004-03-02 2005-09-15 Orion Genomics Llc Differential enzymatic fragmentation by whole genome amplification

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JP4887154B2 (ja) 2012-02-29
AU2004286235A1 (en) 2005-05-12
US8163485B2 (en) 2012-04-24
EP2292795A3 (de) 2011-06-08
WO2005042704A3 (en) 2005-06-30
EP2339024B1 (de) 2014-12-31
EP2292795A2 (de) 2011-03-09
US20050158739A1 (en) 2005-07-21
US20100240064A1 (en) 2010-09-23
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JP2007522795A (ja) 2007-08-16
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CA2542542A1 (en) 2005-05-12
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AU2004286235B2 (en) 2011-05-12
ES2382780T3 (es) 2012-06-13
ATE550438T1 (de) 2012-04-15
WO2005040399A3 (en) 2005-07-21
US8361719B2 (en) 2013-01-29
EP2292795B1 (de) 2015-01-07
AU2004284456A1 (en) 2005-05-06
EP1682677A4 (de) 2007-12-19
EP1682677A2 (de) 2006-07-26
EP2339024A1 (de) 2011-06-29
US7910296B2 (en) 2011-03-22
WO2005040399A2 (en) 2005-05-06
US20050153316A1 (en) 2005-07-14
US20120100535A1 (en) 2012-04-26
EP1689887A4 (de) 2007-12-19
EP1689887B1 (de) 2012-03-21
CA2542526A1 (en) 2005-05-06
EP1682677B1 (de) 2012-04-11
EP1689887A2 (de) 2006-08-16

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