ATE554189T1 - Hochsensibles schnelles isothermisches verfahren zur detektion von punktmutationen und snps - Google Patents
Hochsensibles schnelles isothermisches verfahren zur detektion von punktmutationen und snpsInfo
- Publication number
- ATE554189T1 ATE554189T1 AT09165252T AT09165252T ATE554189T1 AT E554189 T1 ATE554189 T1 AT E554189T1 AT 09165252 T AT09165252 T AT 09165252T AT 09165252 T AT09165252 T AT 09165252T AT E554189 T1 ATE554189 T1 AT E554189T1
- Authority
- AT
- Austria
- Prior art keywords
- point mutations
- snps
- detecting point
- highly sensitive
- isothermal method
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 230000035772 mutation Effects 0.000 title abstract 3
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 101150009057 JAK2 gene Proteins 0.000 abstract 1
- 230000001404 mediated effect Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 238000006116 polymerization reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Viewfinders (AREA)
- Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09165252A EP2272978B1 (de) | 2009-07-10 | 2009-07-10 | Hochsensibles schnelles isothermisches Verfahren zur Detektion von Punktmutationen und SNPs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE554189T1 true ATE554189T1 (de) | 2012-05-15 |
Family
ID=41398176
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT09165252T ATE554189T1 (de) | 2009-07-10 | 2009-07-10 | Hochsensibles schnelles isothermisches verfahren zur detektion von punktmutationen und snps |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US9404149B2 (de) |
| EP (2) | EP2272978B1 (de) |
| JP (1) | JP5841939B2 (de) |
| AT (1) | ATE554189T1 (de) |
| BR (1) | BR112012000604A2 (de) |
| DK (1) | DK2272978T3 (de) |
| ES (2) | ES2386514T3 (de) |
| WO (1) | WO2011003690A1 (de) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103459612A (zh) * | 2010-12-03 | 2013-12-18 | 布兰代斯大学 | 用于检测野生型群体中的核酸突变体的方法和试剂盒 |
| WO2012174192A2 (en) * | 2011-06-14 | 2012-12-20 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | Method of amplifying a nucleic acid |
| JP2013017395A (ja) * | 2011-07-07 | 2013-01-31 | Toyobo Co Ltd | 骨髄増殖性疾患に関する遺伝子変異を検出するためのプローブおよび該プローブを用いた遺伝子変異の検出方法 |
| EP3012326A4 (de) * | 2013-03-26 | 2016-12-21 | Nippon Gene Co Ltd | Primer und sondenset zur identifizierung von genpolymorphismus und verwendung davon |
| US11332782B2 (en) * | 2016-08-05 | 2022-05-17 | Arizona Board Of Regents On Behalf Of Arizona State University | Method for detecting nucleotide polymorphisms |
| CN107841536A (zh) * | 2017-10-24 | 2018-03-27 | 复旦大学附属华山医院 | 一种检测jak2 v617f基因突变的引物组合物及其试剂盒和检测方法 |
| GB201809449D0 (en) * | 2018-06-08 | 2018-07-25 | Imperial Innovations Ltd | Method for detecting a single nucleotide polymorphism(SNP) |
| GB201809420D0 (en) | 2018-06-08 | 2018-07-25 | Imperial Innovations Ltd | Devices and method for detecting an amplification event |
| CN110172500B (zh) * | 2019-05-31 | 2023-12-15 | 中国科学院成都生物研究所 | 一种单核苷酸多态性的等温分型方法 |
| CN111187805B (zh) * | 2020-01-19 | 2023-06-23 | 陕西师范大学 | 一种辅助突变引物的设计方法及其应用 |
| WO2025083239A1 (en) * | 2023-10-20 | 2025-04-24 | Dx4Life Ab | Methods and polynucleotides and uses thereof |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1020534B2 (de) | 1998-11-09 | 2011-01-05 | Eiken Kagaku Kabushiki Kaisha | Verfahren zur synthese von nukleinsäuren |
| US6277607B1 (en) * | 1999-05-24 | 2001-08-21 | Sanjay Tyagi | High specificity primers, amplification methods and kits |
| DE60025840T2 (de) | 1999-11-08 | 2006-07-27 | Eiken Kagaku K.K. | Methode zum nachweis von variationen oder polymorphismen |
| FR2877013A1 (fr) | 2004-10-27 | 2006-04-28 | Assist Publ Hopitaux De Paris | Indentification d'une mutation de jak2 impliquee dans la polyglobulie de vaquez |
| US20070128620A1 (en) * | 2005-07-15 | 2007-06-07 | Applera Corporation | Hot start reverse transcription by primer design |
| US7488581B2 (en) | 2006-03-20 | 2009-02-10 | Kabushiki Kaisha Toshiba | Method for detecting a target nucleic acid sequence |
| JP4410269B2 (ja) | 2007-03-28 | 2010-02-03 | 株式会社東芝 | Ｎ−アセチルトランスフェラーゼ２（ｎａｔ２）の遺伝子型を検出するための核酸プライマーセット、キット、及び該プライマーセットを用いた検出方法 |
| WO2009049630A1 (en) | 2007-10-16 | 2009-04-23 | Aarhus Universitet | Isothermal amplification method for detection of nucleic acid mutation |
| JP2009106220A (ja) * | 2007-10-31 | 2009-05-21 | Genodive Pharma Kk | 基板上での等温増幅反応による標的塩基配列の捕捉及び検出方法 |
| US20100310563A1 (en) | 2007-11-30 | 2010-12-09 | Bumm Thomas G P | Methods for treating induced cellular proliferative disorders |
-
2009
- 2009-07-10 AT AT09165252T patent/ATE554189T1/de active
- 2009-07-10 EP EP09165252A patent/EP2272978B1/de not_active Not-in-force
- 2009-07-10 DK DK09165252.9T patent/DK2272978T3/da active
- 2009-07-10 ES ES09165252T patent/ES2386514T3/es active Active
-
2010
- 2010-06-08 BR BR112012000604-5A patent/BR112012000604A2/pt not_active Application Discontinuation
- 2010-06-08 JP JP2012518855A patent/JP5841939B2/ja active Active
- 2010-06-08 WO PCT/EP2010/058022 patent/WO2011003690A1/en not_active Ceased
- 2010-06-08 ES ES10725083.9T patent/ES2535178T3/es active Active
- 2010-06-08 US US13/383,141 patent/US9404149B2/en active Active
- 2010-06-08 EP EP10725083.9A patent/EP2451968B8/de active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2272978A9 (de) | 2011-10-19 |
| EP2272978B1 (de) | 2012-04-18 |
| EP2451968B1 (de) | 2015-01-21 |
| ES2535178T3 (es) | 2015-05-06 |
| US20120171676A1 (en) | 2012-07-05 |
| WO2011003690A8 (en) | 2011-06-30 |
| WO2011003690A1 (en) | 2011-01-13 |
| ES2386514T3 (es) | 2012-08-22 |
| US9404149B2 (en) | 2016-08-02 |
| JP5841939B2 (ja) | 2016-01-13 |
| EP2272978A1 (de) | 2011-01-12 |
| EP2451968B8 (de) | 2015-04-08 |
| JP2012531919A (ja) | 2012-12-13 |
| EP2451968A1 (de) | 2012-05-16 |
| BR112012000604A2 (pt) | 2020-08-11 |
| DK2272978T3 (da) | 2012-07-23 |
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