ATE555219T1 - Korrekturlese-primerverlängerung - Google Patents

Korrekturlese-primerverlängerung

Info

Publication number
ATE555219T1
ATE555219T1 AT09777762T AT09777762T ATE555219T1 AT E555219 T1 ATE555219 T1 AT E555219T1 AT 09777762 T AT09777762 T AT 09777762T AT 09777762 T AT09777762 T AT 09777762T AT E555219 T1 ATE555219 T1 AT E555219T1
Authority
AT
Austria
Prior art keywords
primer extension
proofreading
primer
reactions
polymerase
Prior art date
Application number
AT09777762T
Other languages
English (en)
Inventor
Ellen Fiss
Thomas Myers
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE555219T1 publication Critical patent/ATE555219T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Joining Of Building Structures In Genera (AREA)
  • Road Signs Or Road Markings (AREA)
  • Reinforcement Elements For Buildings (AREA)
AT09777762T 2008-08-12 2009-08-08 Korrekturlese-primerverlängerung ATE555219T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18884108P 2008-08-12 2008-08-12
PCT/EP2009/005770 WO2010017932A1 (en) 2008-08-12 2009-08-08 Proofreading primer extension

Publications (1)

Publication Number Publication Date
ATE555219T1 true ATE555219T1 (de) 2012-05-15

Family

ID=41323468

Family Applications (1)

Application Number Title Priority Date Filing Date
AT09777762T ATE555219T1 (de) 2008-08-12 2009-08-08 Korrekturlese-primerverlängerung

Country Status (8)

Country Link
US (1) US7989168B2 (de)
EP (1) EP2324124B1 (de)
JP (1) JP5596684B2 (de)
CN (1) CN102119226B (de)
AT (1) ATE555219T1 (de)
CA (1) CA2733903C (de)
ES (1) ES2384681T3 (de)
WO (1) WO2010017932A1 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2646549B1 (de) * 2010-12-02 2015-02-18 Keygene N.V. Gezielte änderung von dna
CN103502449B (zh) * 2010-12-02 2016-08-10 关键基因股份有限公司 用寡核苷酸靶向改变dna
CA2839964C (en) 2011-07-28 2019-03-12 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
US10378051B2 (en) * 2011-09-29 2019-08-13 Illumina Cambridge Limited Continuous extension and deblocking in reactions for nucleic acids synthesis and sequencing
US9133509B2 (en) 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
JP2014000015A (ja) * 2012-06-15 2014-01-09 Kaneka Corp 核酸解析法
EP2943590A4 (de) 2013-01-13 2017-02-15 Unitaq Bio Verfahren und zusammensetzungen für pcr mittels blockierter und universelle primer
CN109517888B (zh) * 2013-04-01 2022-07-26 基因特力株式会社 使用等位基因特异的反应性引物的核酸扩增方法
CN104561249A (zh) * 2013-10-22 2015-04-29 常州金麦格生物技术有限公司 在样品中检测靶核酸的方法
CN104561248A (zh) * 2013-10-22 2015-04-29 常州金麦格生物技术有限公司 用于检测靶核酸的引物和其应用
US10240195B2 (en) 2014-03-24 2019-03-26 The Trustees Of Columbia University In The City Of New York Chemical methods for producing tagged nucleotides
CN107208143A (zh) 2014-11-20 2017-09-26 安普里怀斯公司 用于核酸扩增和分析的组合物、方法、系统和试剂盒
CN104560972A (zh) * 2014-11-28 2015-04-29 深圳市海普洛斯生物科技有限公司 一种pcr引物及其修饰方法及基于平行高通量单分子高保真扩增方法
US11286518B2 (en) 2016-05-06 2022-03-29 Regents Of The University Of Minnesota Analytical standards and methods of using same
GB201919186D0 (en) 2019-12-23 2020-02-05 Biofidelity Ltd Simplified polynucleotide sequence detection method
IL293936A (en) * 2019-12-23 2022-08-01 Biofidelity Ltd kits and devices
EP4288557A4 (de) * 2021-02-02 2024-12-25 RhoDx, Inc. Synthetische polynukleotide und verfahren zur selektiven amplifikation von allelen
WO2024049358A1 (en) * 2022-08-31 2024-03-07 Agency For Science, Technology And Research A method of detecting the presence of a nucleic acid

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6279681B1 (en) * 1998-05-11 2001-08-28 X-It Products, Llc Collapsible ladder having highly nesting rungs with integral stand-off projections
JP3883476B2 (ja) * 1999-03-19 2007-02-21 タカラバイオ株式会社 核酸配列の増幅方法
ATE362532T1 (de) * 1999-03-19 2007-06-15 Takara Bio Inc Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers
AU7125401A (en) 2000-05-19 2001-12-03 David J Marshall Materials and methods for detection of nucleic acids
JP4481656B2 (ja) * 2002-02-27 2010-06-16 ライフ テクノロジーズ コーポレーション ３’→５’エキソヌクレアーゼ耐性クエンチャードメインを含むｆｅｔ標識オリゴヌクレオチドを使用する方法およびそれを実施するための組成物
US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
JP2007501005A (ja) * 2003-08-01 2007-01-25 インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド ポリマー核酸を増幅するための方法
US7928207B2 (en) 2004-06-28 2011-04-19 Roche Molecular Systems, Inc Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides
US20060105348A1 (en) * 2004-11-15 2006-05-18 Lee Jun E Compositions and methods for the detection and discrimination of nucleic acids
DE202008005749U1 (de) 2008-04-25 2008-07-10 Dmg Microset Gmbh Spannvorrichtung zum Einspannen einer Werkzeugeinheit

Also Published As

Publication number Publication date
WO2010017932A1 (en) 2010-02-18
WO2010017932A4 (en) 2010-04-15
US7989168B2 (en) 2011-08-02
EP2324124B1 (de) 2012-04-25
EP2324124A1 (de) 2011-05-25
CN102119226B (zh) 2013-10-30
JP2011530301A (ja) 2011-12-22
ES2384681T3 (es) 2012-07-10
CN102119226A (zh) 2011-07-06
CA2733903C (en) 2013-10-22
JP5596684B2 (ja) 2014-09-24
CA2733903A1 (en) 2010-02-18
US20100041053A1 (en) 2010-02-18
HK1159192A1 (en) 2012-07-27

Similar Documents

Publication Publication Date Title
NZ701145A (en) Compositions and methods for quantifying a nucleic acid sequence in a sample
CY1119331T1 (el) Ισοθερμικη ενισχυση νουκλεικων οξεων
EP4582805A3 (de) Verfahren zur erzeugung von bibliotheken von nukleinsäuresequenzen zur detektion mittels in-situ-fluoreszenzsequenzierung
ATE550441T1 (de) Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
WO2008002920A3 (en) Methods for generating target nucleic acid sequences
MX379233B (es) Métodos y composiciones para el perfilado de adn.
EP4644568A3 (de) Verfahren zur gezielten bildung von nukleinsäurebibliotheken
WO2009012246A3 (en) Nicking and extension amplification reaction for the exponential amplification of nucleic acids
EP4105644A3 (de) Systeme und vorrichtungen zur "sequence-by-synthesis"-analyse
MX353840B (es) Deteccion de acidos nucleicos.
EP2032714A4 (de) Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation
WO2014182528A3 (en) Multiplex labeling of molecules by sequential hybridization barcoding
GB2470672A (en) Methods of RNA aplification in the presence of DNA
WO2008074328A3 (en) Microrna target site blocking oligos and uses thereof
MX2009010710A (es) Complejos y enzimas de acido nucleico y metodos para su uso.
ATE480644T1 (de) Verfahren zur quantifizierung kleiner rna- moleküle
BR112015023398A8 (pt) Métodos para amplificar ácidos nucléicos
CY1118903T1 (el) Συστημα ανιχνευσης αντιδρασης αλυσιδας πολυμερασης χρησιμοποιωντας ολιγονουκλεοτιδια που περιλαμβανουν μια φωσφοροθειοϊκη ομαδα
EP4219519A3 (de) Kompetitive sonden zur erzeugung von techniksignalen
EP4428528A3 (de) Markierte nukleotidzusammensetzungen und verfahren zur nukleinsäuresequenzierung
BRPI0816047A2 (pt) conjunto de oligonucleotídeos para detecção de um ácido nucleico alvo, métodos para detecção da presença ou ausência de um ácido nucleico alvo e de pelo menos dois ácidos nucleicos alvo, kit, mistura de reação e amplicon identificado por fluorescência
ATE544871T1 (de) Hochmultiplexe nukleinsäureamplifikation
EP3656873A3 (de) Polynukleotidanreicherung und -amplifikation unter verwendung von argonautensystemen
EP2004852A4 (de) Amplifikation von dna-fragmenten
ATE534657T1 (de) Neue nachweissonde