ATE75775T1 - Verfahren zur herstellung von biologisch aktivem plasminogenaktivator. - Google Patents

Verfahren zur herstellung von biologisch aktivem plasminogenaktivator.

Info

Publication number
ATE75775T1
ATE75775T1 AT87304995T AT87304995T ATE75775T1 AT E75775 T1 ATE75775 T1 AT E75775T1 AT 87304995 T AT87304995 T AT 87304995T AT 87304995 T AT87304995 T AT 87304995T AT E75775 T1 ATE75775 T1 AT E75775T1
Authority
AT
Austria
Prior art keywords
plasminogen activator
production
biologically active
medium
culture
Prior art date
Application number
AT87304995T
Other languages
English (en)
Inventor
William R Arathoon
Stuart E Builder
Anthony S Lubiniecki
Reis Robert D Van
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE75775T1 publication Critical patent/ATE75775T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT87304995T 1986-06-06 1987-06-05 Verfahren zur herstellung von biologisch aktivem plasminogenaktivator. ATE75775T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US87164286A 1986-06-06 1986-06-06
EP87304995A EP0248675B1 (de) 1986-06-06 1987-06-05 Verfahren zur Herstellung von biologisch aktivem Plasminogenaktivator

Publications (1)

Publication Number Publication Date
ATE75775T1 true ATE75775T1 (de) 1992-05-15

Family

ID=25357832

Family Applications (1)

Application Number Title Priority Date Filing Date
AT87304995T ATE75775T1 (de) 1986-06-06 1987-06-05 Verfahren zur herstellung von biologisch aktivem plasminogenaktivator.

Country Status (21)

Country Link
US (1) US5053334A (de)
EP (1) EP0248675B1 (de)
JP (1) JPS6332484A (de)
KR (1) KR930002888B1 (de)
AT (1) ATE75775T1 (de)
AU (1) AU601984B2 (de)
DD (1) DD257087A5 (de)
DE (2) DE3718939C2 (de)
DK (1) DK175366B1 (de)
ES (1) ES2032444T3 (de)
FI (1) FI91886C (de)
FR (1) FR2599625B1 (de)
GB (1) GB2191493B (de)
GR (1) GR3005245T3 (de)
HU (1) HU205171B (de)
IE (1) IE60485B1 (de)
IL (1) IL82746A (de)
NO (1) NO170595C (de)
NZ (1) NZ220547A (de)
PT (1) PT84991B (de)
ZA (1) ZA874054B (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL82746A (en) * 1986-06-06 1994-10-07 Genentech Inc Process for producing biologically active tissue-type plasminogen activator
JP2576200B2 (ja) * 1988-03-09 1997-01-29 味の素株式会社 生理活性タンパク質の製造法
EP0338716A3 (de) * 1988-04-21 1990-04-11 Berlex Laboratories, Inc. Methode und Vorrichtung zur Produktion nichtdegradierter Proteine aus Säugetierzellen
DE3829244A1 (de) * 1988-08-29 1990-03-01 Boehringer Mannheim Gmbh Verfahren zur herstellung von einkettigem t-pa
EP0391080A3 (de) * 1989-03-07 1990-11-07 Roche Diagnostics GmbH Verfahren zur Herstellung von zweikettigem t-PA
GB2249099B (en) * 1990-09-26 1995-05-03 Squibb & Sons Inc Squalene synthetase
US5677184A (en) * 1994-04-19 1997-10-14 Takeda Chemical Industries, Ltd. CHO cells that express human LH-RH receptor
US6077940A (en) * 1997-12-24 2000-06-20 Genentech, Inc. Free solution ligand interaction molecular separation method
CA2853267C (en) * 2002-04-08 2018-06-26 Octane Biotech Inc. Automated tissue engineering system comprising sensors linked to a microprocessor
DK2634242T3 (en) 2006-07-14 2017-05-22 Patheon Holdings I B V IMPROVED PROCEDURE FOR CULTURING CELLS
ES2657055T3 (es) * 2007-08-09 2018-03-01 Wyeth Llc Uso de perfusión para mejorar la producción de un cultivo de células alimentado por lotes en biorreactores
WO2011091248A1 (en) * 2010-01-22 2011-07-28 Lonza Walkersville, Inc. High yield method and apparatus for volume reduction and washing of therapeutic cells using tangential flow filtration
US10934519B2 (en) * 2011-07-29 2021-03-02 Global Life Sciences Solutions Usa Llc Systems, methods and control laws for cell harvesting
CN104583386A (zh) * 2012-08-20 2015-04-29 泰尔茂比司特公司 浓缩穿过细胞生长腔室循环的流体的组分
CA3248436A1 (en) 2017-09-01 2025-02-21 Lonza Walkersville, Inc. END-TO-END AUTOMATION OF CELL THERAPY
JP7396777B2 (ja) 2018-09-28 2023-12-12 オクタン バイオテック インコーポレーテッド 磁気分離
CA3123449A1 (en) 2018-12-21 2020-06-25 Lonza Walkersville, Inc. Automated production of viral vectors
JP7477091B2 (ja) 2018-12-21 2024-05-01 オクタン バイオテック インコーポレーテッド モジュラー生物製剤生産ユニットのためのカルーセル
KR102810534B1 (ko) 2018-12-28 2025-05-22 옥테인 바이오테크 인코포레이티드 제어된 환경 존을 포함하는 세포 배양 및 조직 공학 시스템
CN113498432A (zh) 2019-02-08 2021-10-12 隆萨沃克斯维尔股份有限公司 用于自动化生物反应器的细胞浓缩方法和装置
IL292409A (en) 2019-10-24 2022-06-01 Octane Biotech Inc A cell culture chamber with enhanced cell contact surfaces
KR20220097457A (ko) 2019-11-11 2022-07-07 론자 워커스빌 아이엔씨. 자동화된 세포 처리를 위한 품질 관리 방법
WO2023278883A1 (en) 2021-07-02 2023-01-05 Combangio, Inc. Processes for making and using a cellular fibronectin composition

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3904480A (en) * 1972-10-24 1975-09-09 Lilly Co Eli Enhanced production of plasminogen activator
JPS5329985A (en) * 1976-08-27 1978-03-20 Kanegafuchi Chem Ind Co Ltd High concentration culturing of yeast
JPS5571496A (en) * 1978-11-24 1980-05-29 Asahi Chem Ind Co Ltd Preparation of living substance
US4409331A (en) * 1979-03-28 1983-10-11 Damon Corporation Preparation of substances with encapsulated cells
US4232124A (en) * 1979-04-23 1980-11-04 Mann George F Method of producing plasminogen activator
DE3015699C2 (de) * 1979-04-26 1982-07-15 Asahi Kasei Kogyo K.K., Osaka Herstellung eines Plasminogen-Aktivators
JPS5642584A (en) * 1979-09-18 1981-04-20 Asahi Chem Ind Co Ltd Cell cultivation method
US4420398A (en) * 1981-08-13 1983-12-13 American National Red Cross Filteration method for cell produced antiviral substances
IL68561A (en) * 1982-05-05 1991-01-31 Genentech Inc Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
JPS5951220A (ja) * 1982-08-02 1984-03-24 Asahi Chem Ind Co Ltd 新規なプラスミノ−ゲン・アクチベ−タ−およびその製法ならびにこれを含有する薬剤
US4537860A (en) * 1982-12-08 1985-08-27 Monsanto Company Static cell culture maintenance system
NZ206699A (en) * 1982-12-30 1989-08-29 Bio Response Inc Process for the production of serum independent cell lines
AU572108B2 (en) * 1983-01-19 1988-05-05 Genentech Inc. Human tpa production using vectors coding for dhfr protein
JPS59121172U (ja) * 1983-02-04 1984-08-15 ミネソタ・マイニング・アンド・マニユフアクチユアリング・コンパニ− ケーブル電線被覆の剥離部分のカバー装置
US4546083A (en) * 1983-04-22 1985-10-08 Stolle Research & Development Corporation Method and device for cell culture growth
GR80966B (en) * 1983-11-21 1985-03-15 Ciba Geigy Ag Synthesis of fibrinolytic agents by yeast
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
US4888115A (en) * 1983-12-29 1989-12-19 Cuno, Incorporated Cross-flow filtration
US4757005A (en) * 1984-04-19 1988-07-12 Miles Inc. Method and cell line for obtaining plasminogen activators
WO1986005514A1 (en) * 1985-03-22 1986-09-25 Chiron Corporation EXPRESSION OF tPA IN MAMMALIAN CELLS
AU593264B2 (en) * 1985-07-10 1990-02-08 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Chromosomal DNA sequence, expression vector for human tissue plasminogen activating factor, cultured cells transfected with same and method of producing said activating factor
EP0238551A4 (de) * 1985-09-06 1988-02-01 Codon Wiedergewinnungsverfahren von gewebeplasminogen-aktivator.
JPH07110232B2 (ja) * 1985-10-14 1995-11-29 株式会社日立製作所 遺伝子組換え大腸菌の遺伝子生産物を採取する方法および装置
IL82746A (en) * 1986-06-06 1994-10-07 Genentech Inc Process for producing biologically active tissue-type plasminogen activator

Also Published As

Publication number Publication date
GB2191493A (en) 1987-12-16
GB2191493B (en) 1991-02-27
KR930002888B1 (ko) 1993-04-12
FI872526A0 (fi) 1987-06-05
ES2032444T3 (es) 1993-02-16
DK175366B1 (da) 2004-09-13
JPH0574346B2 (de) 1993-10-18
KR880000590A (ko) 1988-03-28
PT84991B (pt) 1990-03-08
IL82746A (en) 1994-10-07
DE3718939C2 (de) 1998-09-17
HUT43875A (en) 1987-12-28
NO170595B (no) 1992-07-27
FR2599625B1 (fr) 1992-03-13
NO872395L (no) 1987-12-07
NZ220547A (en) 1990-01-29
ZA874054B (en) 1989-02-22
AU7387287A (en) 1987-12-10
EP0248675A1 (de) 1987-12-09
IL82746A0 (en) 1987-12-20
IE871504L (en) 1987-12-06
JPS6332484A (ja) 1988-02-12
GB8713267D0 (en) 1987-07-08
DD257087A5 (de) 1988-06-01
NO170595C (no) 1992-11-04
DK288187D0 (da) 1987-06-04
AU601984B2 (en) 1990-09-27
US5053334A (en) 1991-10-01
HU205171B (en) 1992-03-30
IE60485B1 (en) 1994-07-27
EP0248675B1 (de) 1992-05-06
FI91886B (fi) 1994-05-13
DE3778758D1 (de) 1992-06-11
FI872526L (fi) 1987-12-07
FR2599625A1 (fr) 1987-12-11
DE3718939A1 (de) 1987-12-17
FI91886C (fi) 1994-08-25
GR3005245T3 (de) 1993-05-24
PT84991A (en) 1987-07-01
DK288187A (da) 1987-12-07
NO872395D0 (no) 1987-06-05

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Legal Events

Date Code Title Description
EELA Cancelled due to lapse of time