ATE69266T1 - Verfahren zum herstellung von insulin-vorlaeufern und verfahren zur herstellung von humaninsulin. - Google Patents

Verfahren zum herstellung von insulin-vorlaeufern und verfahren zur herstellung von humaninsulin.

Info

Publication number: ATE69266T1
Authority: AT; Austria
Prior art keywords: production; methods; insulin; human insulin; precursors
Prior art date: 1985-03-22

Application number

AT86302133T

Other languages

English (en)

Inventor

Lars Thim

Kjeld Norris

Mogens Trier Hansen

Original Assignee

Novo Nordisk As

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-03-22

Filing date

1986-03-21

Publication date

1991-11-15

1986-03-21 Application filed by Novo Nordisk As filed Critical Novo Nordisk As

1991-11-15 Application granted granted Critical

1991-11-15 Publication of ATE69266T1 publication Critical patent/ATE69266T1/de

Links

NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 title abstract 6
101000976075 Homo sapiens Insulin Proteins 0.000 title abstract 5
PBGKTOXHQIOBKM-FHFVDXKLSA-N insulin (human) Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H]1CSSC[C@H]2C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@H](C(N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)N[C@@H](CSSC[C@H](NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3C=CC(O)=CC=3)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC=3NC=NC=3)NC(=O)[C@H](CO)NC(=O)CNC1=O)C(=O)NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O)=O)CSSC[C@@H](C(N2)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](NC(=O)CN)[C@@H](C)CC)[C@@H](C)CC)[C@@H](C)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC=1C=CC=CC=1)C(C)C)C1=CN=CN1 PBGKTOXHQIOBKM-FHFVDXKLSA-N 0.000 title abstract 5
239000002243 precursor Substances 0.000 title abstract 5
102000004877 Insulin Human genes 0.000 title abstract 3
108090001061 Insulin Proteins 0.000 title abstract 3
229940125396 insulin Drugs 0.000 title abstract 3
238000000034 method Methods 0.000 title 2
240000004808 Saccharomyces cerevisiae Species 0.000 abstract 2
239000004475 Arginine Substances 0.000 abstract 1
239000004472 Lysine Substances 0.000 abstract 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 abstract 1
108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 abstract 1
238000012258 culturing Methods 0.000 abstract 1
230000002255 enzymatic effect Effects 0.000 abstract 1
239000001963 growth medium Substances 0.000 abstract 1
238000011084 recovery Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Mycology (AREA)
Engineering & Computer Science (AREA)
Biochemistry (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Zoology (AREA)
Molecular Biology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
Wood Science & Technology (AREA)
Microbiology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Medicinal Chemistry (AREA)
General Engineering & Computer Science (AREA)
Gastroenterology & Hepatology (AREA)
Biomedical Technology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Toxicology (AREA)
Endocrinology (AREA)
Diabetes (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)
Ultra Sonic Daignosis Equipment (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Management, Administration, Business Operations System, And Electronic Commerce (AREA)

AT86302133T 1985-03-22 1986-03-21 Verfahren zum herstellung von insulin-vorlaeufern und verfahren zur herstellung von humaninsulin. ATE69266T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DK129385A DK129385A (da)	1985-03-22	1985-03-22	Peptider og fremstilling deraf
EP86302133A EP0195691B1 (de)	1985-03-22	1986-03-21	Verfahren zum Herstellung von Insulin-Vorläufern und Verfahren zur Herstellung von Humaninsulin

Publications (1)

Publication Number	Publication Date
ATE69266T1 true ATE69266T1 (de)	1991-11-15

Family

ID=8103207

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT86302133T ATE69266T1 (de)	1985-03-22	1986-03-21	Verfahren zum herstellung von insulin-vorlaeufern und verfahren zur herstellung von humaninsulin.

Country Status (16)

Country	Link
EP (1)	EP0195691B1 (de)
JP (3)	JPH0648993B2 (de)
AT (1)	ATE69266T1 (de)
AU (1)	AU586855B2 (de)
CA (1)	CA1340823C (de)
DE (1)	DE3682303D1 (de)
DK (1)	DK129385A (de)
ES (2)	ES8802402A1 (de)
FI (1)	FI87801C (de)
GR (1)	GR860764B (de)
IE (1)	IE58842B1 (de)
MX (1)	MX163837B (de)
NO (1)	NO172646C (de)
NZ (1)	NZ215570A (de)
PT (1)	PT82241B (de)
ZA (1)	ZA861953B (de)

Families Citing this family (43)

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DK58285D0 (da) *	1984-05-30	1985-02-08	Novo Industri As	Peptider samt fremstilling og anvendelse deraf
ATE69062T1 (de) *	1985-06-12	1991-11-15	Lubrizol Genetics Inc	Modifiziertes zein.
PH25772A (en) *	1985-08-30	1991-10-18	Novo Industri As	Insulin analogues, process for their preparation
DK437786D0 (da) *	1986-09-12	1986-09-12	Nordisk Gentofte	Insulinprecursorer
US5426036A (en) *	1987-05-05	1995-06-20	Hoechst Aktiengesellschaft	Processes for the preparation of foreign proteins in streptomycetes
DE3837271A1 (de) *	1988-11-03	1990-05-10	Hoechst Ag	Verfahren zur selektiven spaltung von fusionsproteinen
CA1340772C (en) *	1987-12-30	1999-09-28	Patricia Tekamp-Olson	Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
DK336188D0 (da) *	1988-06-20	1988-06-20	Nordisk Gentofte	Propeptider
ATE101620T1 (de) *	1988-06-23	1994-03-15	Hoechst Ag	Mini-proinsulin, seine herstellung und verwendung.
US6875589B1 (en)	1988-06-23	2005-04-05	Hoechst Aktiengesellschaft	Mini-proinsulin, its preparation and use
ATE132531T1 (de) *	1988-11-03	1996-01-15	Hoechst Ag	Verfahren zur herstellung eines insulin- vorprodukts in streptomyceten
US5716927A (en) *	1988-12-23	1998-02-10	Novo Nordisk A/S	Insulin analogs having a modified B-chain
DE3844211A1 (de) *	1988-12-29	1990-07-05	Hoechst Ag	Neue insulinderivate, verfahren zu deren herstellung, ihre verwendung und eine sie enthaltende pharmazeutische zubereitung
IL93282A (en) *	1989-02-09	1995-08-31	Lilly Co Eli	Insulin analogues
DK134189D0 (da) *	1989-03-20	1989-03-20	Nordisk Gentofte	Insulinforbindelser
CA2051375C (en) *	1989-05-04	2000-09-12	Dennis J. Henner	Process and compositions for the isolation of human relaxin
FI103805B1 (fi) *	1990-09-05	1999-09-30	Hoechst Ag	Menetelmä preproinsuliinien hydroloimiseksi
NZ245170A (en) *	1991-11-26	1994-07-26	Lilly Co Eli	Insulin and proinsulin analogues with arg at b31, b32 and ao and pharmaceutical compositions
FR2686899B1 (fr)	1992-01-31	1995-09-01	Rhone Poulenc Rorer Sa	Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
US6001604A (en) *	1993-12-29	1999-12-14	Bio-Technology General Corp.	Refolding of proinsulins without addition of reducing agents
NZ279002A (en) *	1994-12-29	1999-02-25	Bio Technology General Corp	Production of recombinant human insulin by folding a proinsulin hybrid polypeptide obtained from a bacterial cell
KR0150565B1 (ko) *	1995-02-15	1998-08-17	김정재	유전자 조환에 의한 사람 인슐린 전구체의 제조 및 이를 이용한 인슐린의 제조방법
IL118127A0 (en) *	1995-05-05	1996-09-12	Lilly Co Eli	Single chain insulin with high bioactivity
GB9513967D0 (en) *	1995-07-08	1995-09-06	Univ Leicester	Insulin
KR100381494B1 (ko) *	1997-06-30	2003-07-22	바이오 테크놀로지 제네랄 코오포레이션	인간인슐린의제조방법
AU9162398A (en) *	1997-08-22	1999-03-16	Boehringer Mannheim Gmbh	Process for producing insulin precursor proteins
DE19930676B4 (de)	1999-07-02	2006-01-19	Sanofi-Aventis Deutschland Gmbh	Verfahren zur Stabilisierung von Insulin, Insulinderivaten und/oder deren Vorläufer in komplexen Mischungen bei deren Lagerung in wäßrigen Lösungsmitteln
JP2003528149A (ja)	2000-03-24	2003-09-24	ジェネンテック・インコーポレーテッド	軟骨性疾患の治療のためのインスリンの使用
JP2003530838A (ja)	2000-04-12	2003-10-21	ヒューマンゲノムサイエンシズインコーポレイテッド	アルブミン融合タンパク質
AR025646A1 (es)	2000-09-13	2002-12-04	Beta Lab Sa	Cepa de levaduras metilotroficas recombinantes productoras de un precursor de insulina, construcciones de adn y metodo para obtener la cepa.
US7202059B2 (en)	2001-02-20	2007-04-10	Sanofi-Aventis Deutschland Gmbh	Fusion proteins capable of being secreted into a fermentation medium
US7638618B2 (en)	2001-02-20	2009-12-29	Sanofi-Aventis Deutschland Gmbh	Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
US7312192B2 (en) *	2001-09-07	2007-12-25	Biocon Limited	Insulin polypeptide-oligomer conjugates, proinsulin polypeptide-oligomer conjugates and methods of synthesizing same
ES2425738T3 (es)	2001-12-21	2013-10-17	Human Genome Sciences, Inc.	Proteínas de fusión de la albúmina
EP1594530A4 (de)	2003-01-22	2006-10-11	Human Genome Sciences Inc	Albuminfusionsproteine
KR20110059602A (ko)	2008-08-07	2011-06-02	바이오콘 리미티드	인슐린 화합물의 제조방법
AR072563A1 (es) *	2009-07-15	2010-09-08	Beta Lab Sa	Un procedimiento para obtener insulina aspartica que utiliza una cepa de levaduras de pichia pastoris
PL239062B1 (pl) *	2016-01-22	2021-11-02	Inst Biotechnologii I Antybiotykow	Sposób wytwarzania insuliny i jej pochodnych
US11230585B2 (en)	2016-09-06	2022-01-25	Chemical & Biopharmaceutical Laboratories Of Patra	Proinsulin derivatives
KR102666154B1 (ko) *	2018-08-08	2024-05-20	주식회사 대웅제약	지속형 인슐린 아날로그 및 그 복합체
KR102646845B1 (ko) *	2018-08-08	2024-03-14	주식회사 대웅제약	클로스트리파인을 이용한 지속형 인슐린 아날로그 복합체의 활성형 제조방법
EP3856141A1 (de) *	2018-09-25	2021-08-04	Amphastar Pharmaceuticals, Inc.	Hochreines api von rekombinantem humanem insulin (rhi) und verfahren zur herstellung davon
KR20200080747A (ko) *	2018-12-27	2020-07-07	주식회사 폴루스	인슐린 전구체의 인슐린 효소 전환용 조성물 및 이를 이용하여 인슐린 전구체를 인슐린으로 전환하는 방법

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
PH16156A (en) *	1980-03-27	1983-07-18	Lilly Co Eli	Process for producing an insulin precursor
NZ199391A (en) *	1981-01-02	1985-12-13	Genentech Inc	Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin
DE3209184A1 (de) *	1982-03-13	1983-09-15	Hoechst Ag, 6230 Frankfurt	Verfahren zur umwandlung von praeproinsulinanaloga zu insulinen
DK172738B1 (da) *	1983-04-07	1999-06-21	Chiron Corp	Fremgangsmåde til at udtrykke modent insulin og DNA-struktur til anvendelse ved fremgangsmåden
DK58285D0 (da) *	1984-05-30	1985-02-08	Novo Industri As	Peptider samt fremstilling og anvendelse deraf

1985
- 1985-03-22 DK DK129385A patent/DK129385A/da not_active Application Discontinuation
1986
- 1986-03-17 ZA ZA861953A patent/ZA861953B/xx unknown
- 1986-03-20 MX MX1947A patent/MX163837B/es unknown
- 1986-03-20 JP JP61060995A patent/JPH0648993B2/ja not_active Expired - Lifetime
- 1986-03-20 CA CA000504650A patent/CA1340823C/en not_active Expired - Fee Related
- 1986-03-21 ES ES553231A patent/ES8802402A1/es not_active Expired
- 1986-03-21 NZ NZ215570A patent/NZ215570A/xx unknown
- 1986-03-21 AT AT86302133T patent/ATE69266T1/de not_active IP Right Cessation
- 1986-03-21 IE IE73786A patent/IE58842B1/en not_active IP Right Cessation
- 1986-03-21 PT PT82241A patent/PT82241B/pt not_active IP Right Cessation
- 1986-03-21 EP EP86302133A patent/EP0195691B1/de not_active Expired - Lifetime
- 1986-03-21 FI FI861198A patent/FI87801C/fi not_active IP Right Cessation
- 1986-03-21 GR GR860764A patent/GR860764B/el unknown
- 1986-03-21 NO NO861120A patent/NO172646C/no not_active IP Right Cessation
- 1986-03-21 DE DE8686302133T patent/DE3682303D1/de not_active Expired - Lifetime
- 1986-03-21 AU AU55005/86A patent/AU586855B2/en not_active Ceased
- 1986-11-28 ES ES557227A patent/ES8708251A1/es not_active Expired
1993
- 1993-05-24 JP JP5121394A patent/JPH088871B2/ja not_active Expired - Lifetime
1995
- 1995-07-14 JP JP7178875A patent/JP2553326B2/ja not_active Expired - Lifetime

Also Published As

Publication number	Publication date
IE58842B1 (en)	1993-11-17
PT82241B (pt)	1988-11-30
JPS61221200A (ja)	1986-10-01
FI861198A0 (fi)	1986-03-21
NO172646C (no)	1993-08-18
EP0195691B1 (de)	1991-11-06
NZ215570A (en)	1989-07-27
DK129385A (da)	1986-09-23
EP0195691A1 (de)	1986-09-24
FI87801B (fi)	1992-11-13
ES557227A0 (es)	1987-10-01
CA1340823C (en)	1999-11-16
AU5500586A (en)	1986-09-25
JPH088871B2 (ja)	1996-01-31
FI87801C (fi)	1993-02-25
ES553231A0 (es)	1988-05-16
DE3682303D1 (de)	1991-12-12
JPH0648993B2 (ja)	1994-06-29
DK129385D0 (da)	1985-03-22
MX163837B (es)	1992-06-25
NO861120L (no)	1986-09-23
ES8802402A1 (es)	1988-05-16
FI861198L (fi)	1986-09-23
NO172646B (no)	1993-05-10
PT82241A (en)	1986-04-01
JPH06133777A (ja)	1994-05-17
ES8708251A1 (es)	1987-10-01
AU586855B2 (en)	1989-07-27
JP2553326B2 (ja)	1996-11-13
GR860764B (en)	1986-07-21
IE860737L (en)	1986-09-22
JPH0823985A (ja)	1996-01-30
ZA861953B (en)	1986-11-26

Legal Events

Date	Code	Title	Description
1992-06-15	UEP	Publication of translation of european patent specification
2004-12-15	REN	Ceased due to non-payment of the annual fee

Publication	Publication Date	Title
ATE69266T1 (de)	1991-11-15	Verfahren zum herstellung von insulin-vorlaeufern und verfahren zur herstellung von humaninsulin.
ATE46358T1 (de)	1989-09-15	Verfahren zur zuechtung von lyophilisierten mikroorganismen.
ATE69241T1 (de)	1991-11-15	Herstellung von polypeptiden mit humangammainterferon-aktivitaet.
ATE75775T1 (de)	1992-05-15	Verfahren zur herstellung von biologisch aktivem plasminogenaktivator.
ATE65797T1 (de)	1991-08-15	Gentechnologisches verfahren zur herstellung von human-interleukin-2 und mittel zur durchfuehrung dieses verfahrens.
DE69333812D1 (de)	2005-06-23	Endo-Xyloglucan-Transferase
DE3751326D1 (de)	1995-07-06	Durch rekombinante DNS-Technologie konstruierte amylolytische enzymproduzierende Mikroorganismen und ihre Verwendung in Gärungsverfahren.
ATE90726T1 (de)	1993-07-15	Verfahren zur herstellung von menschlichem lysozym.
DE69522876D1 (de)	2001-10-31	Verfahren zur Herstellung von L-leucin
DE68924222D1 (de)	1995-10-19	Megakaryocytopoietin, seine Herstellung und Benutzung.
ATE89455T1 (de)	1993-06-15	Mittel zur bekaempfung von clostridien.
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