ATE81529T1 - Rekombinante fusionsproteine. - Google Patents

Rekombinante fusionsproteine.

Info

Publication number: ATE81529T1
Authority: AT; Austria
Prior art keywords: factor; vector; protein; cleavage site; product
Prior art date: 1984-05-16

Application number

AT85303414T

Other languages

English (en)

Inventor

Kiyoshi Nagai

Hans Christian Thogersen

Original Assignee

Celltech Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-05-16

Filing date

1985-05-15

Publication date

1992-10-15

1985-05-15 Application filed by Celltech Ltd filed Critical Celltech Ltd

1992-10-15 Application granted granted Critical

1992-10-15 Publication of ATE81529T1 publication Critical patent/ATE81529T1/de

Links

102000007056 Recombinant Fusion Proteins Human genes 0.000 title 1
108010008281 Recombinant Fusion Proteins Proteins 0.000 title 1
108090000623 proteins and genes Proteins 0.000 abstract 5
102000004169 proteins and genes Human genes 0.000 abstract 4
108010074860 Factor Xa Proteins 0.000 abstract 3
238000003776 cleavage reaction Methods 0.000 abstract 3
108090000765 processed proteins & peptides Proteins 0.000 abstract 3
230000007017 scission Effects 0.000 abstract 3
108020001507 fusion proteins Proteins 0.000 abstract 2
102000037865 fusion proteins Human genes 0.000 abstract 2
PGOHTUIFYSHAQG-LJSDBVFPSA-N (2S)-6-amino-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-4-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-1-[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-1-[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]propanoyl]pyrrolidine-2-carbonyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-4-methylpentanoyl]amino]acetyl]amino]-3-hydroxypropanoyl]amino]-4-methylpentanoyl]amino]-3-sulfanylpropanoyl]amino]-4-methylsulfanylbutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-hydroxybutanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-hydroxypropanoyl]amino]-3-hydroxypropanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-4-methylpentanoyl]amino]-3-hydroxybutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-5-oxopentanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]-5-oxopentanoyl]amino]-3-phenylpropanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-4-oxobutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-4-carboxybutanoyl]amino]-5-oxopentanoyl]amino]hexanoic acid Chemical compound CSCC[C@H](N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCCN)C(O)=O PGOHTUIFYSHAQG-LJSDBVFPSA-N 0.000 abstract 1
108091026890 Coding region Proteins 0.000 abstract 1
108020004705 Codon Proteins 0.000 abstract 1
108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
238000000034 method Methods 0.000 abstract 1
230000001131 transforming effect Effects 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12N9/1033—Chloramphenicol O-acetyltransferase (2.3.1.28)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4716—Muscle proteins, e.g. myosin, actin
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/585—Calcitonins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
- C07K14/805—Haemoglobins; Myoglobins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Molecular Biology (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Medicinal Chemistry (AREA)
Wood Science & Technology (AREA)
Biophysics (AREA)
Biomedical Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Gastroenterology & Hepatology (AREA)
Biotechnology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Microbiology (AREA)
Toxicology (AREA)
Physics & Mathematics (AREA)
Endocrinology (AREA)
Plant Pathology (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Saccharide Compounds (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)

AT85303414T 1984-05-16 1985-05-15 Rekombinante fusionsproteine. ATE81529T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GB848412517A GB8412517D0 (en)	1984-05-16	1984-05-16	Recombinant fusion proteins
EP85303414A EP0161937B1 (de)	1984-05-16	1985-05-15	Rekombinante Fusionsproteine

Publications (1)

Publication Number	Publication Date
ATE81529T1 true ATE81529T1 (de)	1992-10-15

Family

ID=10561052

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT85303414T ATE81529T1 (de)	1984-05-16	1985-05-15	Rekombinante fusionsproteine.

Country Status (9)

Country	Link
US (1)	US6010883A (de)
EP (1)	EP0161937B1 (de)
JP (1)	JPH074253B2 (de)
AT (1)	ATE81529T1 (de)
AU (1)	AU585857B2 (de)
CA (1)	CA1340381C (de)
DE (1)	DE3586750T2 (de)
DK (1)	DK171278B1 (de)
GB (2)	GB8412517D0 (de)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB8412517D0 (en) *	1984-05-16	1984-06-20	Nagai K	Recombinant fusion proteins
US5411873A (en) *	1984-05-29	1995-05-02	Genencor, Inc.	Process for producing heterologous polypeptides
EP0207044A1 (de) *	1985-06-20	1986-12-30	Monsanto Company	Freisetzung von Peptiden aus Polypeptiden
US5087564A (en) *	1985-06-20	1992-02-11	Monsanto Company	Release of recombinant peptides from polypeptides using V8 endopeptidase
US5496924A (en) *	1985-11-27	1996-03-05	Hoechst Aktiengesellschaft	Fusion protein comprising an interleukin-2 fragment ballast portion
DE3636903A1 (de) *	1985-12-21	1987-07-02	Hoechst Ag	Fusionsproteine mit eukaryotischem ballastanteil
US5089406A (en) *	1987-01-07	1992-02-18	Allied-Signal Inc.	Method of producing a gene cassette coding for polypeptides with repeating amino acid sequences
CA1340522C (en) *	1987-03-10	1999-05-04	Heinz Dobeli	Fusion proteins containing neighbouring histidines for improved purification
US5013653A (en)	1987-03-20	1991-05-07	Creative Biomolecules, Inc.	Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
US5215896A (en) *	1987-03-20	1993-06-01	Creative Biomolecules, Inc.	Leader sequences for the production of recombinant proteins
DE3731874A1 (de) *	1987-09-18	1989-03-30	Schering Ag	Verfahren zur herstellung von peptiden durch spezifische spaltung von gentechnisch gewonnenen fusionsproteinen mit collagenasen
IT1223577B (it) *	1987-12-22	1990-09-19	Eniricerche Spa	Procedimento migliorato per la preparazione dell'ormone della crescita umano naturale in forma pura
DE3804148A1 (de) *	1988-02-11	1989-08-24	Andreas Dr Plueckthun	Verfahren zur spaltung von fusionsproteinen
KR920701434A (ko)	1989-05-10	1992-08-11	원본미기재	박테리아 및 이스트 내에서의 헤모글로빈 및 그의 동족체의 제조방법
US5599907A (en)	1989-05-10	1997-02-04	Somatogen, Inc.	Production and use of multimeric hemoglobins
US5545727A (en) *	1989-05-10	1996-08-13	Somatogen, Inc.	DNA encoding fused di-alpha globins and production of pseudotetrameric hemoglobin
US5580560A (en) *	1989-11-13	1996-12-03	Novo Nordisk A/S	Modified factor VII/VIIa
GB8927722D0 (en) *	1989-12-07	1990-02-07	British Bio Technology	Proteins and nucleic acids
DE4009268A1 (de) *	1990-03-22	1991-09-26	Consortium Elektrochem Ind	Sekretion von hirudinderivaten
EP0538398A4 (en) *	1990-06-25	1993-12-29	Oncogene Science, Inc.	Tissue-derived tumor growth inhibitors, methods for preparation and uses thereof
US6514730B1 (en)	1991-03-21	2003-02-04	Consortium für elektrochemische Industrie GmbH	Secretion of hirudin derivatives
JP3277523B2 (ja) *	1991-09-13	2002-04-22	株式会社日立製作所	因子ＸａリンカーＤＮＡ
US5679777A (en) *	1991-11-08	1997-10-21	Somatogen, Inc.	Hemoglobins as drug delivery agents
NZ261571A (en) *	1993-02-04	1997-03-24	Denzyme Aps	Producing correctly folded proteins using multiple denaturation and renaturation cycles
GB9406974D0 (en) *	1994-04-08	1994-06-01	Pharmaceutical Proteins Ltd	Transgenic production
DE19653498C1 (de) *	1996-12-20	1998-07-23	Wolf M Prof Dr Bertling	Klonierungsvektor-System mit Fusionssequenz
US20030207402A1 (en) *	1997-08-22	2003-11-06	Erhard Kopetzki	Autocatalytically activatable zymogenic precursors of proteases and their use
JP2003520571A (ja) *	1999-06-02	2003-07-08	ノボザイムスアクティーゼルスカブ	ポリペプチドの発現及び分泌のためのペクチン酸リアーゼ
HK1048828A1 (zh) *	1999-11-19	2003-04-17	Transkaryotic Therapies, Inc.	可令生产优化的核酸构造
DE10122140A1 (de) *	2001-05-08	2002-11-28	Apotech Res & Dev Ltd	Rekombinante Fusionsproteine und deren Trimere
ES2427974T3 (es) *	2003-04-23	2013-11-05	F. Hoffmann-La Roche Ag	Corte de proteínas de fusión mediante la utilización de la proteasa granzima B
PL213309B1 (pl)	2006-02-09	2013-02-28	Polska Akademia Nauk Inst Biochemii I Biofizyki	Sposób hydrolizy wiazania peptydowego
US8099316B2 (en) *	2007-07-09	2012-01-17	Velti Plc	Mobile device marketing and advertising platforms, methods, and systems
CA2703263C (en)	2007-11-01	2014-03-18	Perseid Therapeutics Llc	Immunosuppressive polypeptides and nucleic acids
US8053222B2 (en) *	2009-02-12	2011-11-08	Academia Sinica, Taiwan	Protein expression system involving mutated severe respiratory syndrome-associated coronavirus 3C-like protease
US9913890B2 (en) *	2012-06-22	2018-03-13	Pharma Green Llc	Methods and compositions for emergency post-infection treatment of anthrax

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US4366246A (en) *	1977-11-08	1982-12-28	Genentech, Inc.	Method for microbial polypeptide expression
IL60184A (en) *	1979-05-31	1984-05-31	Schering Ag	Process for the specific cleavage of protein sequences from proteins
CA1200773A (en) *	1980-02-29	1986-02-18	William J. Rutter	Expression linkers
US4532207A (en) *	1982-03-19	1985-07-30	G. D. Searle & Co.	Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
EP0095361B1 (de) *	1982-05-25	1989-07-26	Eli Lilly And Company	Klonbildungsvektoren für die Expression von exogenem Protein
WO1984001173A1 (en) *	1982-09-15	1984-03-29	Immuno Nuclear Corp	Production of mature proteins in transformed yeast
JPS60500043A (ja) *	1982-12-10	1985-01-17	ノルデイスク・インスリンラボラトリウム	原核細胞又は真核細胞中で合成される融合蛋白質から成熟蛋白質を作る方法
SE8300693L (sv) *	1983-02-09	1984-08-10	Sven Lofdahl	Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta
GB8314362D0 (en) *	1983-05-24	1983-06-29	Celltech Ltd	Polypeptide and protein products
GB2140810B (en) *	1983-05-24	1987-07-08	Celltech Ltd	Polypeptide and protein products, and process for their production
JPS60501758A (ja) *	1983-07-01	1985-10-17	セルテク　リミテツド	タンパク質、医薬構成物、遺伝子、ベクタ−宿主生物及びそれらの生産方法
GB8412517D0 (en) *	1984-05-16	1984-06-20	Nagai K	Recombinant fusion proteins
US4713482A (en) *	1985-03-26	1987-12-15	Ciba-Geigy Corporation	Maleic or phthalic acid half esters of alkoxylated fatty amines
EP0213472A1 (de) *	1985-08-12	1987-03-11	Syntex (U.S.A.) Inc.	Verfahren zur Herstellung von Fusionsproteinen

1984
- 1984-05-16 GB GB848412517A patent/GB8412517D0/en active Pending
1985
- 1985-05-14 AU AU42474/85A patent/AU585857B2/en not_active Ceased
- 1985-05-15 DE DE8585303414T patent/DE3586750T2/de not_active Expired - Lifetime
- 1985-05-15 AT AT85303414T patent/ATE81529T1/de not_active IP Right Cessation
- 1985-05-15 CA CA000481611A patent/CA1340381C/en not_active Expired - Fee Related
- 1985-05-15 EP EP85303414A patent/EP0161937B1/de not_active Expired
- 1985-05-15 DK DK217985A patent/DK171278B1/da not_active IP Right Cessation
- 1985-05-15 GB GB08512333A patent/GB2160206B/en not_active Expired
- 1985-05-16 JP JP60104914A patent/JPH074253B2/ja not_active Expired - Lifetime
1992
- 1992-03-19 US US07/854,749 patent/US6010883A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
DE3586750D1 (de)	1992-11-19
GB2160206B (en)	1987-11-11
DE3586750T2 (de)	1993-04-22
EP0161937A3 (en)	1987-10-07
EP0161937B1 (de)	1992-10-14
AU4247485A (en)	1985-11-21
JPH074253B2 (ja)	1995-01-25
JPS61135591A (ja)	1986-06-23
GB8512333D0 (en)	1985-06-19
DK217985D0 (da)	1985-05-15
EP0161937A2 (de)	1985-11-21
GB2160206A (en)	1985-12-18
CA1340381C (en)	1999-02-09
DK171278B1 (da)	1996-08-19
DK217985A (da)	1985-11-17
US6010883A (en)	2000-01-04
AU585857B2 (en)	1989-06-29
GB8412517D0 (en)	1984-06-20

Legal Events

Date	Code	Title	Description
1993-04-15	UEP	Publication of translation of european patent specification
1994-08-15	REN	Ceased due to non-payment of the annual fee

Publication	Publication Date	Title
AU585857B2 (en)	1989-06-29	Recombinant fusion proteins
Wise et al.	1990	Expression of a human proprotein processing enzyme: correct cleavage of the von Willebrand factor precursor at a paired basic amino acid site.
NZ232742A (en)	1991-10-25	Yeast heterologous protein production method, vectors, dna constructs and proteins so produced and aprotinin (1-58) so produced
ATE88501T1 (de)	1993-05-15	Rekombinante dns-expressionsvektoren.
ATE123520T1 (de)	1995-06-15	Kex2-endoprotease und verfahren zu ihrer herstellung.
HUT43629A (en)	1987-11-30	Process for producing fusion proteins
DE3855084D1 (de)	1996-04-18	Herstellung und Reinigung eines Proteins, das mit einem Bindungsprotein fusioniert ist
JPS6410999A (en)	1989-01-13	Production of physiologically active peptide containing cysteine residue
US5759815A (en)	1998-06-02	Production of platelet derived growth factor (PDGF) an muteins thereof
EP0528686B8 (de)	2004-01-14	Verfahren zur Herstellung von Peptiden
Can et al.	1989	High-level expression in Escherichia coli of a chemically synthesized gene for [Leu-28] echistatin
ES2083352T3 (es)	1996-04-16	Produccion de proteinas activas que contienen residuos de cisteina.
EP0477200A1 (de)	1992-04-01	Reinigung von proteinen mittels ctap-iii schmelzvorgänge
ATE46188T1 (de)	1989-09-15	Positionsspezifische proteolyse mittels renin.
EA199700073A1 (ru)	1997-12-30	Новые производные стафилокиназы
ATE415422T1 (de)	2008-12-15	Menschliches gas-6 (growth arrest-specific gene 6) protein und dafür kodierende nukleinsäuren
IT1186750B (it)	1987-12-16	Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni
EP1241180A3 (de)	2003-03-19	Menschliches A33-Antigen-ähnliches Protein und dafür kodierende Nukleinsäure
PT1241251E (pt)	2008-03-25	Proteína semelhante a prostasina humana e ácidos nucleicos que a codificam
DK1241184T3 (da)	2008-12-08	Humant synaptogyrin-lignende protein og nukleinsyrer kodende for dette
DE69943058D1 (de)	2011-02-03	Loslösendes protein 5 - ucp5