ES2083352T3 - Produccion de proteinas activas que contienen residuos de cisteina. - Google Patents

Produccion de proteinas activas que contienen residuos de cisteina.

Info

Publication number
ES2083352T3
ES2083352T3 ES86115148T ES86115148T ES2083352T3 ES 2083352 T3 ES2083352 T3 ES 2083352T3 ES 86115148 T ES86115148 T ES 86115148T ES 86115148 T ES86115148 T ES 86115148T ES 2083352 T3 ES2083352 T3 ES 2083352T3
Authority
ES
Spain
Prior art keywords
production
cysteine residues
proteins containing
nucleotide sequence
active proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES86115148T
Other languages
English (en)
Inventor
Robert T Garvin
Eric James
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cangene Corp
Original Assignee
Cangene Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cangene Corp filed Critical Cangene Corp
Application granted granted Critical
Publication of ES2083352T3 publication Critical patent/ES2083352T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

SE DESCRIBE UNA SECUENCIA DE DNA PARA CODIFICAR PROTEINAS QUE TENGAN RESTOS DE CISTINA, QUE IMPLICA UNA SECUENCIA NUCLEOTIDA REGULADORA, SELECCIONADA DE UNA CELULA O UN MICROORGANISMO CAPAZ DE FORMAR Y EXCRETAR PROTEINAS HOMOLOGAS UNIDAS POR ENLACES DISULFURO, DICHA SECUENCIA NUCLEOTIDA UNIDA CON FINES DE OPERACION A UNA SEGUNDA SECUENCIA DE NUCLEOTIDO CODIFICADA PARA ORIGINAR PROTEINAS HETEROLOGAS QUE CONTIENEN UN ENLACE DISULFURO. TAMBIEN SE DESCRIBE UN PROCEDIMIENTO PARA PRODUCIR UNA PROTEINA QUE CONTIENE CISTINA.
ES86115148T 1985-11-01 1986-10-31 Produccion de proteinas activas que contienen residuos de cisteina. Expired - Lifetime ES2083352T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA000494456A CA1295563C (en) 1985-11-01 1985-11-01 Production of active proteins containing cystine residues

Publications (1)

Publication Number Publication Date
ES2083352T3 true ES2083352T3 (es) 1996-04-16

Family

ID=4131785

Family Applications (1)

Application Number Title Priority Date Filing Date
ES86115148T Expired - Lifetime ES2083352T3 (es) 1985-11-01 1986-10-31 Produccion de proteinas activas que contienen residuos de cisteina.

Country Status (5)

Country Link
EP (1) EP0222279B1 (es)
AT (1) ATE133711T1 (es)
CA (1) CA1295563C (es)
DE (1) DE3650477T2 (es)
ES (1) ES2083352T3 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1295566C (en) * 1987-07-21 1992-02-11 Robert T. Garvin Characterization and structure of genes for protease a and protease b from streptomyces griseus
CA1295567C (en) * 1988-07-25 1992-02-11 Lawrence T. Malek Expression system for the secretion of bioactive human granulocyte, macrophage-colony stimulating factor (gm-csf) and other heterologous proteins from streptomyces
WO1988007079A1 (en) * 1987-03-09 1988-09-22 Cetus Corporation Expression of heterologous genes in streptomyces species
US5610034A (en) * 1987-04-29 1997-03-11 Alko Group Ltd. Immunoglobulin production by trichoderma
DE3908813A1 (de) * 1989-03-17 1990-09-20 Roehm Gmbh Verfahren zur expression eines aus aspergillus niger stammenden gens in einem aspergillus
GB9106185D0 (en) * 1991-03-22 1991-05-08 Wellcome Found Biological control agents
FR2686899B1 (fr) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
US6926898B2 (en) 2000-04-12 2005-08-09 Human Genome Sciences, Inc. Albumin fusion proteins
CA2841097A1 (en) 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin and g-csf fusion proteins
WO2005003296A2 (en) 2003-01-22 2005-01-13 Human Genome Sciences, Inc. Albumin fusion proteins

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3418274A1 (de) * 1984-05-17 1985-11-21 Hoechst Ag, 6230 Frankfurt Signalpeptid fuer die exkretion von peptiden in streptomyceten
US4745056A (en) * 1984-10-23 1988-05-17 Biotechnica International, Inc. Streptomyces secretion vector

Also Published As

Publication number Publication date
DE3650477D1 (de) 1996-03-14
EP0222279A2 (en) 1987-05-20
EP0222279B1 (en) 1996-01-31
EP0222279A3 (en) 1988-11-09
DE3650477T2 (de) 1996-10-31
ATE133711T1 (de) 1996-02-15
CA1295563C (en) 1992-02-11

Similar Documents

Publication Publication Date Title
AU595221B2 (en) Fusion proteins, a process for their preparation and their use
JPS6485096A (en) Hybrid human protein c and biotechnological synthesis thereof
DE3751269D1 (de) Expression des biologisch aktiven Faktors XIII.
FI104261B1 (fi) Menetelmä yhdistelmä ihmisen erytropoietiinin tuottamiseksi
ATE81529T1 (de) Rekombinante fusionsproteine.
FI883082A0 (fi) Konstruering av syntetisk dna och dess anvaendning i syntes av stora polypeptider.
HUT46061A (en) Dna sequences encoding hirudin-like protein and process for producing such proteins
NO872988L (no) Pattedyr-interleukin-4.
FI875427A0 (fi) Menetelmä valmistaa hevosen -interferonia
NZ242492A (en) Method of improving yield of disulphide-bonded proteins by using thiol reagents
DE59611434D1 (de) Herstellung von Proteinen aus Pro-Proteinen durch Fusionsproteine abgeleitet von Furin oder Furinanalogen
DE3650477D1 (de) Produktion von Cystinreste enthaltenden aktiven Proteinen
PT83968A (de) Verfahren zur verbesserung des expression von eukaryontenprotein
EP0206200A3 (en) Human cholinesterase-type proteins and their production
AU1090688A (en) Synthetic porcine growth hormone
ATE87977T1 (de) Herstellung menschlichen somatomedins c.
EP0213085A3 (en) Vector for the expression and secretion of heterologous gene products in bacillus subtilis
DE58909692D1 (de) Verfahren zur selektiven Spaltung von Fusionsproteinen
FI934079L (fi) Menetelmä fuusioproteiinin valmistamiseksi

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 222279

Country of ref document: ES