AU2004282577B2 - Site-specific incorporation of redox active amino acids into proteins - Google Patents

Site-specific incorporation of redox active amino acids into proteins Download PDF

Info

Publication number
AU2004282577B2
AU2004282577B2 AU2004282577A AU2004282577A AU2004282577B2 AU 2004282577 B2 AU2004282577 B2 AU 2004282577B2 AU 2004282577 A AU2004282577 A AU 2004282577A AU 2004282577 A AU2004282577 A AU 2004282577A AU 2004282577 B2 AU2004282577 B2 AU 2004282577B2
Authority
AU
Australia
Prior art keywords
trna
amino acid
cell
redox active
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2004282577A
Other languages
English (en)
Other versions
AU2004282577A1 (en
Inventor
Lital Alfonta
Peter G. Schultz
Zhiwen Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scripps Research Institute
Original Assignee
Scripps Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Institute filed Critical Scripps Research Institute
Publication of AU2004282577A1 publication Critical patent/AU2004282577A1/en
Application granted granted Critical
Publication of AU2004282577B2 publication Critical patent/AU2004282577B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93Ligases (6)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU2004282577A 2003-10-14 2004-10-13 Site-specific incorporation of redox active amino acids into proteins Ceased AU2004282577B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US51153203P 2003-10-14 2003-10-14
US60/511,532 2003-10-14
PCT/US2004/034089 WO2005038002A2 (en) 2003-10-14 2004-10-13 Site-specific incorporation of redox active amino acids into proteins

Publications (2)

Publication Number Publication Date
AU2004282577A1 AU2004282577A1 (en) 2005-04-28
AU2004282577B2 true AU2004282577B2 (en) 2009-10-29

Family

ID=34465242

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2004282577A Ceased AU2004282577B2 (en) 2003-10-14 2004-10-13 Site-specific incorporation of redox active amino acids into proteins

Country Status (14)

Country Link
US (5) US7494796B2 (2)
EP (1) EP1687401B1 (2)
JP (1) JP5657850B2 (2)
CN (2) CN1942579B (2)
AU (1) AU2004282577B2 (2)
BR (1) BRPI0415308A (2)
CA (1) CA2541166A1 (2)
DK (1) DK1687401T3 (2)
ES (1) ES2396712T3 (2)
IL (3) IL174947A (2)
MX (1) MXPA06004087A (2)
PL (1) PL1687401T3 (2)
PT (1) PT1687401E (2)
WO (1) WO2005038002A2 (2)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002085923A2 (en) * 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids
MXPA05003978A (es) * 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
EP2410331B1 (en) 2003-06-18 2015-09-23 The Scripps Research Institute Aminoacyl-tRNA synthetase for aminoacylation tRNA with unnatural amino acids
EP1649004A4 (en) * 2003-07-07 2008-04-09 Scripps Research Inst COMPOSITIONS WITH PAIRS OF ORTHOGONAL LYSYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF
JP2008500050A (ja) * 2004-05-25 2008-01-10 ザ スクリップス リサーチ インスティテュート 結晶構造決定のための重原子含有非天然アミノ酸の部位特異的蛋白質組込み
DE102004031258A1 (de) * 2004-06-29 2006-02-09 Jennissen, Herbert P., Prof. Dr. Proteinhybride mit polyhydroxyaromatischen Aminosäure-Epitopen
US20080171317A1 (en) * 2004-09-22 2008-07-17 The Scripps Research Institute Site-Specific Labeling of Proteins for Nmr Studies
CN101048506B (zh) 2004-10-27 2013-06-26 斯克利普斯研究院 体内掺入非天然氨基酸的正交翻译组分
BRPI0617191A2 (pt) * 2005-10-12 2011-07-19 Scripps Research Inst modificação pós-traducional de polipeptìdeos expressos em fagos
WO2007092475A2 (en) * 2006-02-06 2007-08-16 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins
KR20080106430A (ko) * 2006-03-09 2008-12-05 더 스크립스 리서치 인스티튜트 진정 세균 숙주 세포 내에서의 직교형 번역 성분의 발현을 위한 시스템
WO2007109035A2 (en) 2006-03-16 2007-09-27 The Scripps Research Institute Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
AU2007267950B2 (en) * 2006-05-23 2012-08-16 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
JP5385143B2 (ja) * 2006-09-21 2014-01-08 ザ スクリプス リサーチ インスティチュート 真正細菌における選択的に硫酸化された蛋白質の遺伝的にプログラムされた発現
WO2008073184A2 (en) 2006-10-18 2008-06-19 The Scripps Research Institute Genetic incorporation of unnatural amino acids into proteins in mammalian cells
WO2008137452A1 (en) * 2007-05-04 2008-11-13 Kenergy Royalty Company, Llc Implantable high efficiency digital stimulation device
US8673591B2 (en) 2007-10-25 2014-03-18 The Scripps Research Institute Genetic incorporation of 3-aminotyrosine into reductases
WO2009059056A2 (en) * 2007-11-02 2009-05-07 The Scripps Research Institute A genetically encoded boronate amino acid
JP5513398B2 (ja) * 2007-11-02 2014-06-04 ザ スクリプス リサーチ インスティチュート 非天然アミノ酸を含有する蛋白質を使用する指向的進化
CN101939410A (zh) 2007-12-11 2011-01-05 斯克利普斯研究院 甲基营养酵母菌巴斯德毕赤酵母中的体内非天然氨基酸表达
EP2249865A4 (en) * 2008-02-08 2012-04-04 Scripps Research Inst BREAKING IMMUNOLOGICAL TOLERANCE WITH A GENETICALLY ENCODED UNNATURAL AMINO ACID
JP2012511403A (ja) * 2008-12-09 2012-05-24 クック メディカル テクノロジーズ エルエルシー 鋲留め器具の制御放出のための装置と方法
US8609383B2 (en) 2008-12-10 2013-12-17 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
WO2010089741A1 (en) * 2009-02-04 2010-08-12 Ben-Gurion University Of The Negev Research And Development Authority Systems and methods for bio-electricity production
JP6574754B2 (ja) 2013-03-19 2019-09-11 ベイジン シェノゲン ファーマ グループ リミテッド エストロゲン受容体関連疾患を処置するための抗体及び方法
US20150331624A1 (en) * 2014-05-19 2015-11-19 Kabushiki Kaisha Toshiba Host-controlled flash translation layer snapshot
KR101956042B1 (ko) * 2017-07-13 2019-03-08 서강대학교산학협력단 표적 단백질 내 l-디하이드록시페닐알라닌의 도입 방법
EP3983501B1 (en) 2019-06-17 2025-02-26 Merck Patent GmbH Liquid crystal-based light valve

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002086075A2 (en) * 2001-04-19 2002-10-31 The Scripps Research Institute Methods and composition for the production of orthoganal trna-aminoacyltrna synthetase pairs

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5879905A (en) * 1996-08-29 1999-03-09 Massachusetts Institute Of Technology In vivo method of initiating protein synthesis with an amino acid other than methionine
MXPA05003978A (es) 2002-10-16 2005-06-22 Scripps Research Inst Sintesis de glicoproteinas.
JP4444113B2 (ja) 2002-10-16 2010-03-31 ザ スクリップス リサーチ インスティテュート ケトアミノ酸の部位特異的蛋白質組込み方法
CA2508939A1 (en) 2002-12-22 2004-07-15 The Scripps Research Institute Protein arrays
JP5642916B2 (ja) 2003-04-17 2014-12-17 ザ スクリプス リサーチ インスティテュート 真核遺伝コードの拡張
EP2410331B1 (en) 2003-06-18 2015-09-23 The Scripps Research Institute Aminoacyl-tRNA synthetase for aminoacylation tRNA with unnatural amino acids
WO2005007624A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof
EP1649004A4 (en) 2003-07-07 2008-04-09 Scripps Research Inst COMPOSITIONS WITH PAIRS OF ORTHOGONAL LYSYL-TRNA AND AMINOACYL-TRNA-SYNTHETASE AND USES THEREOF
US20060160175A1 (en) 2003-07-07 2006-07-20 The Scripps Research Institute Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002086075A2 (en) * 2001-04-19 2002-10-31 The Scripps Research Institute Methods and composition for the production of orthoganal trna-aminoacyltrna synthetase pairs
US20090068717A1 (en) * 2001-04-19 2009-03-12 The Scripps Research Institute In vivo incorporation of unnatural amino acids

Also Published As

Publication number Publication date
DK1687401T3 (da) 2013-02-04
ES2396712T3 (es) 2013-02-25
US20120100570A1 (en) 2012-04-26
PL1687401T3 (pl) 2013-04-30
BRPI0415308A (pt) 2006-12-05
IL174947A0 (en) 2006-08-20
CN101532046A (zh) 2009-09-16
US9783798B2 (en) 2017-10-10
CA2541166A1 (en) 2005-04-28
CN1942579A (zh) 2007-04-04
US8114654B2 (en) 2012-02-14
WO2005038002A2 (en) 2005-04-28
EP1687401B1 (en) 2012-11-21
US20050227318A1 (en) 2005-10-13
CN1942579B (zh) 2013-06-26
IL201705A0 (en) 2011-08-01
JP2007508034A (ja) 2007-04-05
US20090298124A1 (en) 2009-12-03
JP5657850B2 (ja) 2015-01-21
US20070009990A1 (en) 2007-01-11
IL206889A0 (en) 2010-12-30
WO2005038002A3 (en) 2006-11-23
EP1687401A2 (en) 2006-08-09
AU2004282577A1 (en) 2005-04-28
IL174947A (en) 2011-11-30
US7494796B2 (en) 2009-02-24
US8008041B2 (en) 2011-08-30
EP1687401A4 (en) 2007-08-01
PT1687401E (pt) 2013-02-26
US7811801B2 (en) 2010-10-12
US20110008782A1 (en) 2011-01-13
MXPA06004087A (es) 2006-06-27

Similar Documents

Publication Publication Date Title
US9783798B2 (en) Site-specific incorporation of redox active amino acids into proteins
EP1666604B1 (en) Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
EP1994165B1 (en) Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
AU2005286722B2 (en) In vivo incorporation of alkynyl amino acids into proteins in eubacteria
EP1991680B1 (en) System for the expression of orthogonal translation components in eubacterial host cells
US9624485B2 (en) Genetic incorporation of unnatural amino acids into proteins in mammalian cells
EP1914314B1 (en) Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired