BE1001973A5 - Expression et purification d'une proteine du gene htlv-iii gag/env. - Google Patents

Expression et purification d'une proteine du gene htlv-iii gag/env. Download PDF

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Publication number
BE1001973A5
BE1001973A5 BE8700350A BE8700350A BE1001973A5 BE 1001973 A5 BE1001973 A5 BE 1001973A5 BE 8700350 A BE8700350 A BE 8700350A BE 8700350 A BE8700350 A BE 8700350A BE 1001973 A5 BE1001973 A5 BE 1001973A5
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BE
Belgium
Prior art keywords
protein
htlv
expression
gag
iii
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BE8700350A
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English (en)
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Hoffmann La Roche
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/14011—Deltaretrovirus, e.g. bovine leukeamia virus
    • C12N2740/14022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/974—Aids related test
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/826—Viruses

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • AIDS & HIV (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Méthodes et compositions pour le clonage et l'expression de plasmides portant des gènes codant pour une nouvelle protéine dérivée de HTLV-III. Cette protéine, qui est appelée protéine gag/env et qui contient des déterminants antigéniques à la fois des protéines de la particule centrale et de l'enveloppe du HTLV-III, peut être purifiée jusqu'à l'état homogène et utilisée comme base pour des tests diagnostiques pour la détection de la présence d'anticorps dirigés contre les virus associés au SIDA ou des virus eux-mêmes dans les sérums humains et d'autres liquides biologiques. La protéine gag/env peut également être préparée pour être utilisée comme vaccin pour la protection contre le SIDA par immunisation préventive.
BE8700350A 1986-04-04 1987-04-03 Expression et purification d'une proteine du gene htlv-iii gag/env. BE1001973A5 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/848,671 US4925784A (en) 1986-04-04 1986-04-04 Expression and purification of an HTLV-III gag/env gene protein

Publications (1)

Publication Number Publication Date
BE1001973A5 true BE1001973A5 (fr) 1990-05-02

Family

ID=25303966

Family Applications (1)

Application Number Title Priority Date Filing Date
BE8700350A BE1001973A5 (fr) 1986-04-04 1987-04-03 Expression et purification d'une proteine du gene htlv-iii gag/env.

Country Status (23)

Country Link
US (1) US4925784A (fr)
JP (1) JP2625118B2 (fr)
KR (1) KR930001118B1 (fr)
AT (1) AT400442B (fr)
AU (1) AU599091B2 (fr)
BE (1) BE1001973A5 (fr)
BR (1) BR8701528A (fr)
CA (1) CA1341249C (fr)
CH (1) CH676004A5 (fr)
DE (3) DE3744825C2 (fr)
DK (1) DK172274B1 (fr)
ES (3) ES2004133A6 (fr)
FR (1) FR2606422B1 (fr)
GB (1) GB2188639B (fr)
IL (1) IL82088A (fr)
IT (1) IT1203851B (fr)
NL (1) NL192275C (fr)
NO (1) NO871409L (fr)
NZ (1) NZ219837A (fr)
SE (1) SE8701413L (fr)
SG (1) SG102792G (fr)
SU (1) SU1644720A3 (fr)
ZA (1) ZA871724B (fr)

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Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
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Non-Patent Citations (2)

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Title
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Also Published As

Publication number Publication date
NO871409L (no) 1987-10-05
ES2016426A6 (es) 1990-11-01
BR8701528A (pt) 1988-01-19
ZA871724B (en) 1987-11-25
NL192275C (nl) 1997-04-03
AU7103287A (en) 1987-10-08
US4925784A (en) 1990-05-15
KR930001118B1 (ko) 1993-02-18
IT1203851B (it) 1989-02-23
DE3744825C2 (fr) 1990-10-25
NL8700795A (nl) 1987-11-02
GB2188639A (en) 1987-10-07
DE3711016A1 (de) 1987-10-08
IT8719973A0 (it) 1987-04-03
JPS62244393A (ja) 1987-10-24
NZ219837A (en) 1990-09-26
FR2606422B1 (fr) 1991-01-04
SU1644720A3 (ru) 1991-04-23
DK172274B1 (da) 1998-02-16
DK166087D0 (da) 1987-04-01
AU599091B2 (en) 1990-07-12
KR870010189A (ko) 1987-11-30
NO871409D0 (no) 1987-04-03
SE8701413L (sv) 1987-10-05
CH676004A5 (fr) 1990-11-30
DE3744827C2 (fr) 1990-08-09
NL192275B (nl) 1996-12-02
ES2004133A6 (es) 1988-12-01
ES2009350A6 (es) 1989-09-16
IL82088A (en) 1991-11-21
JP2625118B2 (ja) 1997-07-02
DK166087A (da) 1987-10-05
FR2606422A1 (fr) 1988-05-13
AT400442B (de) 1995-12-27
IL82088A0 (en) 1987-10-30
SG102792G (en) 1993-01-29
GB8707971D0 (en) 1987-05-07
DE3711016C2 (fr) 1990-08-02
CA1341249C (fr) 2001-06-12
SE8701413D0 (sv) 1987-04-03
ATA82487A (de) 1995-05-15
GB2188639B (en) 1990-09-26

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