BE1002908A4 - Procede pour obtenir des polydesoxyribonucleotides chimiquement definis et reproductibles. - Google Patents

Procede pour obtenir des polydesoxyribonucleotides chimiquement definis et reproductibles. Download PDF

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Publication number
BE1002908A4
BE1002908A4 BE8700423A BE8700423A BE1002908A4 BE 1002908 A4 BE1002908 A4 BE 1002908A4 BE 8700423 A BE8700423 A BE 8700423A BE 8700423 A BE8700423 A BE 8700423A BE 1002908 A4 BE1002908 A4 BE 1002908A4
Authority
BE
Belgium
Prior art keywords
polydesoxyribonucleotides
reproducible
chemically defined
depolymerization
obtaining chemically
Prior art date
Application number
BE8700423A
Other languages
English (en)
Inventor
Gianfranco Fedeli
Giuseppe Diamantini
Marisa Mantovani
Giuseppe Prino
Original Assignee
Crinos Industria Farmaco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Crinos Industria Farmaco filed Critical Crinos Industria Farmaco
Application granted granted Critical
Publication of BE1002908A4 publication Critical patent/BE1002908A4/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/117—Nucleic acids having immunomodulatory properties, e.g. containing CpG-motifs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/17—Immunomodulatory nucleic acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

En effectuant la dépolymérisation de solutions stabilisées de polydésoxyribonucléotides fortement polymérisés et coupés, obtenus par agrégation stabilisante d'acides nucléiques bruts, la dépolymérisation étant effectuée par chauffage à une température réglée et étant contrôlée en fonction de la variation de l'hyperchomicité réversible, puis par élimination des liaisions hydrogène dans les filaments à deux brins, et par stabilisation thermique les filaments à un seul brin, on obtient le polydésoxyribonucléotide appelé Défibrotide.
BE8700423A 1986-04-17 1987-04-17 Procede pour obtenir des polydesoxyribonucleotides chimiquement definis et reproductibles. BE1002908A4 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT20117/86A IT1190313B (it) 1986-04-17 1986-04-17 Procedimento per l'ottenimento di polidesossiribonucleotidi chimicamente definiti e riproducibili e prodotto farmacologicamente attivo risultante

Publications (1)

Publication Number Publication Date
BE1002908A4 true BE1002908A4 (fr) 1991-07-30

Family

ID=11163944

Family Applications (1)

Application Number Title Priority Date Filing Date
BE8700423A BE1002908A4 (fr) 1986-04-17 1987-04-17 Procede pour obtenir des polydesoxyribonucleotides chimiquement definis et reproductibles.

Country Status (20)

Country Link
US (1) US4985552A (fr)
EP (1) EP0263155B1 (fr)
JP (1) JP2581722B2 (fr)
KR (1) KR880701242A (fr)
CN (1) CN1018650B (fr)
AR (1) AR242585A1 (fr)
AT (1) ATE66927T1 (fr)
AU (1) AU612976B2 (fr)
BE (1) BE1002908A4 (fr)
CA (1) CA1297430C (fr)
CH (1) CH677114A5 (fr)
DE (1) DE3772697D1 (fr)
ES (1) ES2005168A6 (fr)
FR (1) FR2597481B1 (fr)
GR (1) GR870602B (fr)
IL (1) IL82251A (fr)
IT (1) IT1190313B (fr)
MX (1) MX168887B (fr)
WO (1) WO1987006235A1 (fr)
ZA (1) ZA872755B (fr)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5182269A (en) * 1987-09-23 1993-01-26 Crinos Industria Farmacobiologica Spa Composition for topical use having hair stimulating, anti-dandruff and anti-seborrhoic activity
IT1252174B (it) * 1991-12-09 1995-06-05 Crinos Industria Farmaco Oligodesossimibonucleotidi ad attivita' antiischemica e procedimenti per il loro ottenimento
IT1271685B (it) * 1994-08-03 1997-06-04 Crinos Industria Farmaco Impiego dei polidesossiribonucleotidi nelle neuropati diabetiche
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
ES2251134T3 (es) * 1999-06-08 2006-04-16 Gentium S.P.A. Uso de complejos entre liposomas cationicos y polidesoxirribonucleotidos como medicamentos.
JP2001078901A (ja) 1999-09-14 2001-03-27 Paloma Ind Ltd 液体加熱調理器
EP1147777A1 (fr) 2000-04-18 2001-10-24 Crinos Industria Farmacobiologica S.p.A. Combinaison de Defibrotide et de G-CSF et son utilisation pour activer les cellules progénitrices haematopoiétiques
WO2002065125A1 (fr) * 2001-02-13 2002-08-22 Invitrogen Corporation Procedes et compositions utilises dans l'isolation de macromolecules biologiques
EP1325962A1 (fr) * 2001-12-17 2003-07-09 Gentium S.p.A. Procédé pour la détermination de l'activité biologique de la defibrotide
US7314746B2 (en) 2002-09-13 2008-01-01 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
EP2216415B2 (fr) * 2003-08-01 2017-01-04 Life Technologies Corporation Compositions et procédés de préparation de courtes molécules d'ARN et d'autres acides nucléiques
ITMI20031714A1 (it) * 2003-09-05 2005-03-06 Gentium Spa Formazioni ad azione antitumorale.
AU2006222045B2 (en) * 2005-03-03 2011-10-20 Gentium Spa Oligodeoxyribonucleotides of 4000-10000 Dalton for treating tumors
EP2103689A1 (fr) * 2008-03-19 2009-09-23 Gentium S.p.A. Oligonucléotides phosphodiesters synthétiques et leurs utilisations thérapeutiques
EP2637672B1 (fr) 2010-11-12 2018-08-22 Gentium S.r.l. Défibrotide pour une utilisation dans la prophylaxie et/ou le traitement de la maladie du greffon contre l'hôte (mgch)
ES2660969T5 (es) * 2012-06-22 2021-09-03 Gentium S R L Método basado en euglobulina para determinar la actividad biológica de defibrotida
EP3026122A1 (fr) 2014-11-27 2016-06-01 Gentium S.p.A. Procédé à base cellulaire pour déterminer la puissance de défibrotide
TW201909904A (zh) 2017-08-03 2019-03-16 愛爾蘭商爵士製藥愛爾蘭有限責任公司 高濃度調配物
CN112236149A (zh) 2018-04-12 2021-01-15 贾兹制药公司 用于预防和治疗细胞因子释放综合征和与免疫耗竭相关的神经毒性的去纤苷
US20190328765A1 (en) 2018-04-30 2019-10-31 Gentium S.R.L. Methods for treating patients having sinusoidal obstruction syndrome
US20220023533A1 (en) 2018-12-07 2022-01-27 Jazz Phrmaceticals Ireland Limited Subcutaneous delivery of high concentration formulations
EP4110287A1 (fr) 2020-02-28 2023-01-04 Jazz Pharmaceuticals Ireland Limited Administration de formulations de faible viscosité
WO2021212055A1 (fr) 2020-04-17 2021-10-21 Jazz Pharmaceuticals Ireland Limited Traitement de défibrotide pour la prévention d'un rejet et d'une lésion d'organe
TW202308659A (zh) 2021-05-06 2023-03-01 愛爾蘭商爵士製藥愛爾蘭有限責任公司 用於急性呼吸窘迫症候群之治療及預防的去纖維蛋白多核苷酸

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2131249A5 (fr) * 1970-11-03 1972-11-10 Crinos Industria Farmaco

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1371944A (fr) * 1963-07-30 1964-09-11 Centre Nat Rech Scient Procédé de préparation d'acides désoxyribonucléiques hautement polymérisés
US3899481A (en) * 1970-11-03 1975-08-12 Crinos Industria Farmaco Process for the controlled partial degradation of deoxyribonucleic acid extracted from animal organs
DE2154279A1 (de) * 1970-11-03 1972-05-25 Crinos Industria Farmaco Medikamente für das fibrinolytische System
CH617963A5 (fr) * 1973-10-05 1980-06-30 Derivati Organici Lab
SU652187A1 (ru) * 1975-07-23 1979-03-15 Киевский Ордена Ленина Государственный Университет Им. Т.Г.Шевченко Способ получени дезоксирибонуклеиновой кислоты
JPS554308A (en) * 1978-06-23 1980-01-12 Yuki Gosei Yakuhin Kogyo Kk Isolation of deoxyribonucleic acid from milt of fish
IT1170215B (it) * 1983-09-12 1987-06-03 Crinos Industria Farmaco Composizione farmaceutica per il trattamento di stati di insufficienza renale acuta
IT1206341B (it) * 1984-02-16 1989-04-14 Crinos Industria Farmaco Composizione farmaceutica per il trattamento dell'ischemia acuta del miocardio.

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2131249A5 (fr) * 1970-11-03 1972-11-10 Crinos Industria Farmaco
FR2131248A5 (fr) * 1970-11-03 1972-11-10 Crinos Industria Farmaco

Also Published As

Publication number Publication date
AR242585A1 (es) 1993-04-30
ATE66927T1 (de) 1991-09-15
IL82251A0 (en) 1987-10-30
MX168887B (es) 1993-06-14
CH677114A5 (fr) 1991-04-15
JPS63503067A (ja) 1988-11-10
ES2005168A6 (es) 1989-03-01
GR870602B (en) 1987-08-17
ZA872755B (en) 1987-10-05
FR2597481B1 (fr) 1990-01-12
IL82251A (en) 1991-05-12
JP2581722B2 (ja) 1997-02-12
CN1018650B (zh) 1992-10-14
AU7288687A (en) 1987-11-09
EP0263155B1 (fr) 1991-09-04
KR880701242A (ko) 1988-07-26
WO1987006235A1 (fr) 1987-10-22
CN87103688A (zh) 1988-01-20
FR2597481A1 (fr) 1987-10-23
DE3772697D1 (de) 1991-10-10
US4985552A (en) 1991-01-15
IT8620117A0 (it) 1986-04-17
EP0263155A1 (fr) 1988-04-13
AU612976B2 (en) 1991-07-25
IT1190313B (it) 1988-02-16
CA1297430C (fr) 1992-03-17
IT8620117A1 (it) 1987-10-17

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Owner name: *GIENTIUM S.P.A.

Effective date: 20031021

RE20 Patent expired

Owner name: *GIENTIUM S.P.A.

Effective date: 20070417