BR0012099A - Transferência citoplásmica para de-diferenciar células recipientes - Google Patents

Transferência citoplásmica para de-diferenciar células recipientes

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Publication number
BR0012099A
BR0012099A BR0012099-5A BR0012099A BR0012099A BR 0012099 A BR0012099 A BR 0012099A BR 0012099 A BR0012099 A BR 0012099A BR 0012099 A BR0012099 A BR 0012099A
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Brazil
Prior art keywords
cells
methods
differentiate
cytoplasm
cytoplasmic transfer
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Application number
BR0012099-5A
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English (en)
Inventor
Karen B Chapman
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Advanced Cell Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Advanced Cell Tech Inc filed Critical Advanced Cell Tech Inc
Publication of BR0012099A publication Critical patent/BR0012099A/pt

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    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer

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  • Animal Behavior & Ethology (AREA)
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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

"TRANSFERêNCIA CITOPLáSMICA PARA DE-DIFERENCIAR CéLULAS RECIPIENTES". Métodos para de-diferenciação ou alteração da vida útil de células "recipientes" desejadas, por exemplo, células somáticas humanas, através de introdução de citoplasma de um tipo de célula menos diferenciado mais primitivo, por exemplo, oócito ou blastómero, são proporcionados. Esses métodos podem ser usados para produzir células do tronco embriónico e aumentar a eficiência da terapia genética permitindo que células desejadas sejam submetidas a modificações genéticas múltiplas sem se tornarem senescentes. Tal citoplasma pode ser fracionado e/ou submetido à hibridização subtrativa e os materiais ativos (suficientes para de-diferenciação) identificados e produzidos por meio de métodos recombinantes.
BR0012099-5A 1999-06-30 2000-06-30 Transferência citoplásmica para de-diferenciar células recipientes BR0012099A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14125099P 1999-06-30 1999-06-30
PCT/US2000/018063 WO2001000650A1 (en) 1999-06-30 2000-06-30 Cytoplasmic transfer to de-differentiate recipient cells

Publications (1)

Publication Number Publication Date
BR0012099A true BR0012099A (pt) 2003-07-29

Family

ID=22494867

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0012099-5A BR0012099A (pt) 1999-06-30 2000-06-30 Transferência citoplásmica para de-diferenciar células recipientes

Country Status (11)

Country Link
US (4) US20020001842A1 (pt)
EP (1) EP1196426A4 (pt)
JP (1) JP2003503071A (pt)
CN (1) CN1362965A (pt)
AU (1) AU782286B2 (pt)
BR (1) BR0012099A (pt)
CA (1) CA2377515C (pt)
IL (2) IL147179A0 (pt)
MX (1) MXPA01013450A (pt)
NZ (1) NZ516236A (pt)
WO (1) WO2001000650A1 (pt)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3880795B2 (ja) 1997-10-23 2007-02-14 ジェロン・コーポレーション フィーダー細胞を含まない培養物中で、霊長類由来始原幹細胞を増殖させるための方法
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
EP1196426A4 (en) 1999-06-30 2003-09-03 Advanced Cell Tech Inc CYTOPLASMATIC TRANSFER FOR DE-DIFFERENTIATION OF VASCELL CELLS
EP1214443A4 (en) * 1999-09-07 2003-08-27 Advanced Cell Tech Inc RESTORATION OF THE TELOMER AND EXTENSION OF THE CELL LIFETIME IN CLONED ANIMALS FROM SENESCENT SOMATIC CELLS
AU1777301A (en) * 1999-11-19 2001-05-30 Hematech, Llc Production of ungulates, preferably bovines that produce human immunoglobulins
US7414170B2 (en) * 1999-11-19 2008-08-19 Kirin Beer Kabushiki Kaisha Transgenic bovines capable of human antibody production
US7253334B2 (en) * 2000-12-22 2007-08-07 Aurox, Llc Methods for cloning non-human mammals using reprogrammed donor chromatin or donor cells
US7820878B2 (en) * 1999-11-19 2010-10-26 Kyowa Hakko Kirin Co., Ltd. Production of ungulates, preferably bovines that produce human immunoglobulins
US7074983B2 (en) * 1999-11-19 2006-07-11 Kirin Beer Kabushiki Kaisha Transgenic bovine comprising human immunoglobulin loci and producing human immunoglobulin
US20050042749A1 (en) 2001-05-16 2005-02-24 Carpenter Melissa K. Dopaminergic neurons and proliferation-competent precursor cells for treating Parkinson's disease
IL152741A0 (en) 2000-05-17 2003-06-24 Geron Corp Neural progenitor cell populations
US20020142397A1 (en) 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
US7491534B2 (en) * 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
US7265262B2 (en) 2001-03-21 2007-09-04 Roslin Institute (Edinburgh) Telomerizing nuclear donor cells and improving the efficiency on nuclear transfer
JP2004526449A (ja) 2001-03-29 2004-09-02 イクシオン・バイオテクノロジー・インコーポレーテッド 非膵性幹細胞の膵分化経路への分化転換法
GB0118984D0 (en) * 2001-08-03 2001-09-26 Intercytex Ltd Fusion of cells
JP2005500847A (ja) * 2001-08-24 2005-01-13 アドバンスド セル テクノロジー、インコーポレイテッド 分化誘導薬の同定のためのスクリーニング検定及び細胞治療用の分化細胞の調製
WO2003018780A1 (en) * 2001-08-27 2003-03-06 Advanced Cell Technology, Inc. De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
WO2003054146A2 (en) 2001-11-14 2003-07-03 Northwestern University Self-assembly and mineralization of peptide-amphiphile nanofibers
WO2003070749A2 (en) 2002-02-15 2003-08-28 Northwestern University Self-assembly of peptide-amphiphile nanofibers under physiological conditions
WO2004013606A2 (en) 2002-08-02 2004-02-12 Stratatech Corporation Species specific dna detection
EP1391503A1 (en) * 2002-08-12 2004-02-25 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts A method of cell re-programming by cytoplasmic transfer
US7534761B1 (en) 2002-08-21 2009-05-19 North Western University Charged peptide-amphiphile solutions and self-assembled peptide nanofiber networks formed therefrom
JP2006505291A (ja) * 2002-11-08 2006-02-16 ヘマテック,エルエルシー プリオンタンパク質活性が低減されたトランスジェニック有蹄動物及びその用途
US7554021B2 (en) 2002-11-12 2009-06-30 Northwestern University Composition and method for self-assembly and mineralization of peptide amphiphiles
US7683025B2 (en) 2002-11-14 2010-03-23 Northwestern University Synthesis and self-assembly of ABC triblock bola peptide amphiphiles
MXPA05008483A (es) 2003-02-11 2006-03-10 Univ Northwestern Metodos y materiales para revestimientos de superficie nanocristalinos y union de nanofibras de anfililos peptidicos sobre las mismas.
AU2004232917A1 (en) * 2003-04-21 2004-11-04 University Of Massachusetts Reprogramming nuclear function with somatic cell cytoplasm
US7683236B2 (en) * 2003-06-10 2010-03-23 Trustees Of The University Of Pennsylvania Enhanced production of cloned mammals by zona pellucida-free homologous mammalian embryo aggregation
GB0316089D0 (en) * 2003-07-09 2003-08-13 Xo Bioscience Ltd Differentiation method
US7823789B2 (en) 2004-12-21 2010-11-02 Cognex Technology And Investment Corporation Low profile illumination for direct part mark readers
FR2861997A1 (fr) * 2003-11-06 2005-05-13 Fred Zacouto Stimulateur cardiaque orthorythmique inotrope
ATE541580T1 (de) 2003-12-05 2012-02-15 Univ Northwestern Selbst anordnende peptid-amphiphile und relevante verfahren für die abgabe von wachstumsfaktor
WO2005056576A2 (en) 2003-12-05 2005-06-23 Northwestern University Branched peptide amphiphiles, related epitope compounds and self assembled structures thereof
US20050214257A1 (en) 2003-12-23 2005-09-29 Northwestern University Compositions and methods for controlling stem cell and tumor cell differentiation, growth, and formation
CA2563064A1 (en) 2004-04-22 2005-11-10 Kirin Beer Kabushiki Kaisha Transgenic animals and uses thereof
CA2598251A1 (en) 2005-03-04 2006-09-14 Northwestern University Angiogenic heparin binding peptide amphiphiles
FR2885368A1 (fr) * 2005-05-04 2006-11-10 Fred Zacouto Systemes et procedes de regeneration cellulaire et utilisations de telles cellules regenerees
WO2007026255A2 (en) * 2005-06-22 2007-03-08 Universitetet I Oslo Dedifferentiated cells and methods of making and using dedifferentiated cells
CA2659945C (en) 2005-08-03 2014-12-16 Advanced Cell Technology, Inc. Improved methods of reprogramming animal somatic cells
US20070087437A1 (en) * 2005-10-14 2007-04-19 Jifan Hu Methods for rejuvenating cells in vitro and in vivo
US8114843B2 (en) 2005-11-18 2012-02-14 The Regents Of The University Of California Photoreactive regulator of protein function and methods of use thereof
WO2008103462A2 (en) 2007-02-23 2008-08-28 Advanced Cell Technology, Inc. Highly efficient methods for reprogramming differentiated cells and for generating animals and embryonic stem cells from reprogrammed cells
US8076295B2 (en) 2007-04-17 2011-12-13 Nanotope, Inc. Peptide amphiphiles having improved solubility and methods of using same
JP2009024027A (ja) * 2008-09-16 2009-02-05 Tadashi Goino 生理活性組成物
US9249412B2 (en) 2009-01-26 2016-02-02 Fred Zacouto Simplified method for partial genetic and epigenetic reprogramming of cells using siRNA specific for a heterochromatin protein 1
CN101798569B (zh) * 2009-02-09 2012-11-21 北京瑞途之星科技有限公司 以卵裂后的发育胚胎替代卵母细胞的治疗性克隆新方法
AU2010236584A1 (en) 2009-04-13 2011-11-10 Northwestern University Novel peptide-based scaffolds for cartilage regeneration and methods for their use
CA2807552A1 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
ES3005233T3 (en) 2010-10-01 2025-03-14 Modernatx Inc Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
US9732128B2 (en) 2010-10-22 2017-08-15 Biotime, Inc. Methods of modifying transcriptional regulatory networks in stem cells
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
WO2013010045A1 (en) 2011-07-12 2013-01-17 Biotime Inc. Novel methods and formulations for orthopedic cell therapy
CN102266350A (zh) * 2011-07-20 2011-12-07 黄必录 逆转干细胞衰老的方案
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
CN102321571A (zh) * 2011-09-21 2012-01-18 王跃嗣 从分化细胞制备自体造血干细胞的方法
EP2763701B1 (en) 2011-10-03 2018-12-19 Moderna Therapeutics, Inc. Modified nucleosides, nucleotides, and nucleic acids, and uses thereof
JP2015501844A (ja) 2011-12-16 2015-01-19 モデルナ セラピューティクス インコーポレイテッドModerna Therapeutics,Inc. 修飾ヌクレオシド、ヌクレオチドおよび核酸組成物
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
WO2013151663A1 (en) 2012-04-02 2013-10-10 modeRNA Therapeutics Modified polynucleotides for the production of membrane proteins
US9254311B2 (en) 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
ES2921623T3 (es) 2012-11-26 2022-08-30 Modernatx Inc ARN modificado terminalmente
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
CA2914615C (en) 2013-06-05 2023-10-17 Biotime, Inc. Compositions and methods for induced tissue regeneration in mammalian species
WO2015048744A2 (en) 2013-09-30 2015-04-02 Moderna Therapeutics, Inc. Polynucleotides encoding immune modulating polypeptides
KR20160067219A (ko) 2013-10-03 2016-06-13 모더나 세라퓨틱스, 인코포레이티드 저밀도 지단백질 수용체를 암호화하는 폴리뉴클레오타이드
US11078462B2 (en) 2014-02-18 2021-08-03 ReCyte Therapeutics, Inc. Perivascular stromal cells from primate pluripotent stem cells
US10240127B2 (en) 2014-07-03 2019-03-26 ReCyte Therapeutics, Inc. Exosomes from clonal progenitor cells
US9371516B2 (en) 2014-09-19 2016-06-21 Regenerative Medical Solutions, Inc. Compositions and methods for differentiating stem cells into cell populations comprising beta-like cells
US9828634B2 (en) 2015-01-22 2017-11-28 Regenerative Medical Solutions, Inc. Markers for differentiation of stem cells into differentiated cell populations
ES2980659T3 (es) 2015-03-23 2024-10-02 Astellas Inst For Regenerative Medicine Ensayos mejorados de potencia de células de epitelio pigmentario retiniano (EPR) humano y progenitores de fotorreceptores
CN108779435B (zh) 2015-12-07 2022-05-03 再生疗法有限公司 用于重新衍生不同的多能干细胞衍生的褐色脂肪细胞的方法
CN106755113B (zh) * 2016-12-29 2020-06-19 赛业(苏州)生物科技有限公司 Es细胞基因打靶技术制备基因修饰小鼠的改进方法
JP2024534952A (ja) * 2021-09-07 2024-09-26 ユバ バイオサイエンシーズ, インコーポレイテッド 卵巣老化、卵巣疾患および状態、ならびにその症状を処置するための組成物および方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6344526A (ja) * 1986-08-12 1988-02-25 Kingo Yoshida 幼若細胞の細胞質・ミトコンドリア・イントロン他注入による若返り長寿法
US5480772A (en) 1993-02-03 1996-01-02 Brandeis University In vitro activation of a nucleus
US5830651A (en) 1995-06-01 1998-11-03 Signal Pharmaceuticals, Inc. Human oligodendroglial progenitor cell line
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
AU2593697A (en) 1996-03-28 1997-10-17 Albert Einstein College Of Medicine Of Yeshiva University Transgenic organisms with altered telomerase activity
US20010012513A1 (en) 1996-08-19 2001-08-09 University Of Massachusetts Embryonic or stem-like cell lines produced by cross species nuclear transplantation
JP2001500725A (ja) * 1996-08-19 2001-01-23 ユニヴァーシティー オヴ マサチューセッツ 種間核移植により製造される胚性または幹細胞様細胞株
US6011197A (en) * 1997-03-06 2000-01-04 Infigen, Inc. Method of cloning bovines using reprogrammed non-embryonic bovine cells
AU8587598A (en) * 1997-07-26 1999-02-16 Wisconsin Alumni Research Foundation Trans-species nuclear transfer
GB9809178D0 (en) * 1998-04-29 1998-07-01 Univ Edinburgh Nuclear reprogramming of somatic cells
WO2000065137A1 (en) * 1999-04-23 2000-11-02 Palacios Boyce Monica Microelectromechanical devices useful for manipulating cells or embryos, kits thereof, methods of making same, and methods of use thereof
US20040199935A1 (en) 1999-06-30 2004-10-07 Chapman Karen B. Cytoplasmic transfer to de-differentiate recipient cells
EP1196426A4 (en) 1999-06-30 2003-09-03 Advanced Cell Tech Inc CYTOPLASMATIC TRANSFER FOR DE-DIFFERENTIATION OF VASCELL CELLS
AU784731B2 (en) * 1999-12-20 2006-06-08 University Of Massachusetts Embryonic or stem-like cells produced by cross species nuclear transplantation
WO2003018780A1 (en) 2001-08-27 2003-03-06 Advanced Cell Technology, Inc. De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies

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WO2001000650A1 (en) 2001-01-04
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EP1196426A1 (en) 2002-04-17
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US9580683B2 (en) 2017-02-28
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