BR0210870A - Aminopeptidase derivada de bacillus licheniformis, gene codificando a aminopeptidase vetor de expressão contendo o gene, transformante e método para preparação destes - Google Patents
Aminopeptidase derivada de bacillus licheniformis, gene codificando a aminopeptidase vetor de expressão contendo o gene, transformante e método para preparação destesInfo
- Publication number
- BR0210870A BR0210870A BR0210870-4A BR0210870A BR0210870A BR 0210870 A BR0210870 A BR 0210870A BR 0210870 A BR0210870 A BR 0210870A BR 0210870 A BR0210870 A BR 0210870A
- Authority
- BR
- Brazil
- Prior art keywords
- aminopeptidase
- expression vector
- gene
- vector containing
- preparing
- Prior art date
Links
- 108090000915 Aminopeptidases Proteins 0.000 title abstract 6
- 102000004400 Aminopeptidases Human genes 0.000 title abstract 6
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 239000013604 expression vector Substances 0.000 title abstract 5
- 241000194108 Bacillus licheniformis Species 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108700026220 vif Genes Proteins 0.000 abstract 2
- 102000002265 Human Growth Hormone Human genes 0.000 abstract 1
- 108010000521 Human Growth Hormone Proteins 0.000 abstract 1
- 239000000854 Human Growth Hormone Substances 0.000 abstract 1
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 238000007796 conventional method Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
"AMINOPEPTIDASE DERIVADA DE BACILLUS LICHENIFORMIS, GENE CODIFICANDO A AMINOPEPTIDASE VETOR DE EXPRESSãO CONTENDO O GENE, TRANSFORMANTE E MéTODO PARA PREPARAçãO DESTES". A presente invenção refere-se a nova aminopeptidase derivada de Bacillus licheniformis, um gene codificando a aminopeptidase, um vetor de expressão contendo o gene, um transformante de célula transfectado com o vetor de expressão e um processo para preparar uma proteína do tipo natural usando estes. Mais particularmente, a presente invenção refere-se a um gene codificando a aminopeptidase que é clonada e produzida usando a técnica de DNA recombinante, um vetor de expressão contendo o gene, um transformante de célula transfectado com o vetor de expressão e uma aminopeptidase recombinante que é necessária para produzir um hormónio de crescimento humano recombinante em uma proteína do tipo natural e pode ser expressa em um alto rendimento mais estável e vantajosamente, comparado com métodos convencionais para a purificação.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20010040268 | 2001-07-06 | ||
| KR10-2002-0030798A KR100477062B1 (ko) | 2001-07-06 | 2002-05-31 | 바실러스 리케니포미스 유래의 신규한 아미노펩티다아제, 이를 코딩하는 유전자, 이 유전자를 포함하는 발현벡터, 이 발현벡터로 형질전환된 형질전환체 및 이를 이용하는 천연형 단백질의 제조방법 |
| PCT/KR2002/001280 WO2003004635A1 (en) | 2001-07-06 | 2002-07-06 | Novel aminopeptidase derived from bacillus licheniformis, gene encoding the aminopeptidase, expression vector containing the gene, transformant and method for preparation thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0210870A true BR0210870A (pt) | 2004-06-22 |
Family
ID=26639209
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR0210870-4A BR0210870A (pt) | 2001-07-06 | 2002-07-06 | Aminopeptidase derivada de bacillus licheniformis, gene codificando a aminopeptidase vetor de expressão contendo o gene, transformante e método para preparação destes |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7098018B2 (pt) |
| EP (1) | EP1404829B1 (pt) |
| JP (1) | JP4580644B2 (pt) |
| AT (1) | ATE380863T1 (pt) |
| BR (1) | BR0210870A (pt) |
| CA (1) | CA2451528C (pt) |
| DK (1) | DK1404829T3 (pt) |
| ES (1) | ES2295370T3 (pt) |
| PL (1) | PL205888B1 (pt) |
| WO (1) | WO2003004635A1 (pt) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5319543B2 (ja) * | 2007-10-29 | 2013-10-16 | 日本ケミカルリサーチ株式会社 | アミノペプチダーゼの製造方法 |
| US20120021469A1 (en) * | 2009-03-27 | 2012-01-26 | Codexis, Inc. | Amidases and methods of their use |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4755465A (en) | 1983-04-25 | 1988-07-05 | Genentech, Inc. | Secretion of correctly processed human growth hormone in E. coli and Pseudomonas |
| DK589785A (da) * | 1985-12-18 | 1987-06-19 | Samuelsson Ernst Gunnar | Peptidpraeparat, fremgangsmaade til fremstilling deraf samt anvendelse af peptidpraeparatet |
| US5108919A (en) | 1988-06-24 | 1992-04-28 | Genentech, Inc. | Dna sequences encoding yeast ubiquitin hydrolase |
| US5126249A (en) | 1989-05-09 | 1992-06-30 | Eli Lilly And Company | Enzymatic removal of a protein amino-terminal sequence |
| KR100369839B1 (ko) * | 1997-02-28 | 2003-06-12 | 주식회사 엘지생명과학 | 바실러스리케니포미스균주에서유래한아미노펩티다제,그의제조방법및이를이용한천연형단백질의제조방법 |
| EP1355931A2 (en) * | 2000-10-06 | 2003-10-29 | Novozymes Biotech, Inc. | Methods for monitoring multiple gene expression |
-
2002
- 2002-07-06 JP JP2003510794A patent/JP4580644B2/ja not_active Expired - Fee Related
- 2002-07-06 WO PCT/KR2002/001280 patent/WO2003004635A1/en not_active Ceased
- 2002-07-06 US US10/481,531 patent/US7098018B2/en not_active Expired - Fee Related
- 2002-07-06 BR BR0210870-4A patent/BR0210870A/pt not_active IP Right Cessation
- 2002-07-06 PL PL367768A patent/PL205888B1/pl not_active IP Right Cessation
- 2002-07-06 CA CA2451528A patent/CA2451528C/en not_active Expired - Fee Related
- 2002-07-06 AT AT02749387T patent/ATE380863T1/de active
- 2002-07-06 ES ES02749387T patent/ES2295370T3/es not_active Expired - Lifetime
- 2002-07-06 EP EP02749387A patent/EP1404829B1/en not_active Expired - Lifetime
- 2002-07-06 DK DK02749387T patent/DK1404829T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| JP2004533263A (ja) | 2004-11-04 |
| JP4580644B2 (ja) | 2010-11-17 |
| ATE380863T1 (de) | 2007-12-15 |
| WO2003004635A1 (en) | 2003-01-16 |
| CA2451528C (en) | 2011-08-16 |
| PL205888B1 (pl) | 2010-06-30 |
| US20040253703A1 (en) | 2004-12-16 |
| EP1404829A4 (en) | 2005-06-08 |
| DK1404829T3 (da) | 2008-03-25 |
| EP1404829B1 (en) | 2007-12-12 |
| CA2451528A1 (en) | 2003-01-16 |
| PL367768A1 (en) | 2005-03-07 |
| ES2295370T3 (es) | 2008-04-16 |
| EP1404829A1 (en) | 2004-04-07 |
| US7098018B2 (en) | 2006-08-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] | ||
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 13A ANUIDADE. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2313 DE 05-05-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |