BR9104965A - Proteina de tabaco,processo para purificar putrescina n-metiltransferase de um extrato de planta de tabaco,moleculas de dna recombinante,vetor,celula de tabaco transgenica cultivada e planta de tabaco - Google Patents

Proteina de tabaco,processo para purificar putrescina n-metiltransferase de um extrato de planta de tabaco,moleculas de dna recombinante,vetor,celula de tabaco transgenica cultivada e planta de tabaco

Info

Publication number
BR9104965A
BR9104965A BR919104965A BR9104965A BR9104965A BR 9104965 A BR9104965 A BR 9104965A BR 919104965 A BR919104965 A BR 919104965A BR 9104965 A BR9104965 A BR 9104965A BR 9104965 A BR9104965 A BR 9104965A
Authority
BR
Brazil
Prior art keywords
tobacco
tobacco plant
methyltranspherase
putrescin
purify
Prior art date
Application number
BR919104965A
Other languages
English (en)
Inventor
Hebert Y Nakatani
Vedpal S Malik
Original Assignee
Philip Morris
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philip Morris filed Critical Philip Morris
Publication of BR9104965A publication Critical patent/BR9104965A/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1003Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007Methyltransferases (general) (2.1.1.)
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/823Nicotiana, e.g. tobacco
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/814Enzyme separation or purification
    • Y10S435/815Enzyme separation or purification by sorption

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
BR919104965A 1990-11-14 1991-11-14 Proteina de tabaco,processo para purificar putrescina n-metiltransferase de um extrato de planta de tabaco,moleculas de dna recombinante,vetor,celula de tabaco transgenica cultivada e planta de tabaco BR9104965A (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/613,160 US5260205A (en) 1990-11-14 1990-11-14 Method of purifying putrescine N-methyltransferase from tobacco plant extract with a polyamine

Publications (1)

Publication Number Publication Date
BR9104965A true BR9104965A (pt) 1992-09-15

Family

ID=24456121

Family Applications (1)

Application Number Title Priority Date Filing Date
BR919104965A BR9104965A (pt) 1990-11-14 1991-11-14 Proteina de tabaco,processo para purificar putrescina n-metiltransferase de um extrato de planta de tabaco,moleculas de dna recombinante,vetor,celula de tabaco transgenica cultivada e planta de tabaco

Country Status (6)

Country Link
US (3) US5260205A (pt)
EP (1) EP0486214A3 (pt)
JP (1) JPH0584075A (pt)
KR (1) KR920009843A (pt)
BR (1) BR9104965A (pt)
TR (1) TR27504A (pt)

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US5260205A (en) * 1990-11-14 1993-11-09 Philip Morris Incorporated Method of purifying putrescine N-methyltransferase from tobacco plant extract with a polyamine
EP0701614A1 (en) * 1993-06-01 1996-03-20 Philip Morris Products Inc. Putrescine n-methyltransferase, recombinant dna molecules encoding putrescine n-methyltransferase, and transgenic tobacco plants with decreased alkaloid content
US7361331B2 (en) * 1996-10-18 2008-04-22 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Agriculture And Agri-Food Plant bioreactors
US6586661B1 (en) 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
US6168807B1 (en) * 1998-07-23 2001-01-02 Les Laboratoires Aeterna Inc. Low molecular weight components of shark cartilage, processes for their preparation and therapeutic uses thereof
DE19926216A1 (de) * 1999-06-09 2001-02-22 Metallgesellschaft Ag Verfahren zur Herstellung von Bariumsulfat, Bariumsulfat und Verwendung des Bariumsulfats
EP1724355A3 (en) * 2000-08-30 2007-05-23 North Carolina State University Transgenic plants containing molecular decoys that alter protein content therein
JP2004533807A (ja) 2000-11-07 2004-11-11 ノース・キャロライナ・ステイト・ユニヴァーシティ プトレッシン−n−メチルトランスフェラーゼプロモーター
CA2364983A1 (en) * 2000-12-13 2002-06-13 John R. Coleman Nucleic acid molecules and polypeptides for catabolism of abscisic acid
CA2449920A1 (en) * 2001-06-08 2002-12-19 Vector Tobacco Ltd. Modifying nicotine and nitrosamine levels in tobacco
US20060157072A1 (en) * 2001-06-08 2006-07-20 Anthony Albino Method of reducing the harmful effects of orally or transdermally delivered nicotine
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
AU2004266694A1 (en) * 2003-08-19 2005-03-03 22Nd Century Limited, Llc Reduced-exposure tobacco products
EP1684603A2 (en) * 2003-10-02 2006-08-02 Vector Tobacco Ltd. Tobacco product labeling system
ATE530656T1 (de) * 2005-02-23 2011-11-15 Univ North Carolina State Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene.
AU2006247739A1 (en) 2005-05-11 2006-11-23 Vector Tobacco Inc. Reduced risk tobacco products and methods of making same
JP2006315017A (ja) * 2005-05-11 2006-11-24 Canon Inc レーザ切断方法および被切断部材
CA2656430C (en) 2006-06-19 2014-08-05 Jonathan E. Page Nucleic acid encoding n-methylputrescine oxidase and uses thereof
KR100899993B1 (ko) 2007-03-07 2009-05-28 한국생명공학연구원 저니코틴 형질전환 담배식물체 및 이의 제조방법
CN101687906A (zh) 2007-05-16 2010-03-31 马里兰大学,大学城 回收叶蛋白的新方法
US20100206317A1 (en) * 2007-09-28 2010-08-19 Vector Tobacco, Inc. Reduced risk tobacco products and use thereof
WO2009064771A2 (en) 2007-11-12 2009-05-22 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
PH12012501393B1 (en) 2010-01-15 2017-10-25 Univ North Carolina State Compositions and methods for minimizing nornicotine synthesis in tobacco
EP2537929B1 (en) * 2010-02-17 2017-04-12 Japan Tobacco, Inc. Plant component regulation factor, and use thereof
US9862923B2 (en) 2010-03-26 2018-01-09 Philip Morris Usa Inc. Cultured tobacco cells as a matrix for consumable products
EP2681321A1 (en) 2011-02-28 2014-01-08 Altria Client Services Inc. Tobacco inbred plants albex1f and albex1ms
US9096864B2 (en) 2011-02-28 2015-08-04 North Carolina State University Tobacco inbred plants NCBEX1F, NCBEX1MS, and NC EX90
US9603335B2 (en) 2013-01-11 2017-03-28 North Carolina State University Tobacco inbred plants K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, CMS NCTG-61 SRC and hybrid NC196 SRC
US9560830B2 (en) 2013-03-05 2017-02-07 North Carolina State University Tobacco inbred and hybrid plants and uses thereof
EP3113603A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
EP3113604A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
EP3113602A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
US10405571B2 (en) 2015-06-26 2019-09-10 Altria Client Services Llc Compositions and methods for producing tobacco plants and products having altered alkaloid levels
EP3801056A4 (en) 2018-05-30 2022-03-16 LeafPro, LLC. PURIFIED SWEET POTATO LEAF PROTEIN CONCENTRATES AND RECOVERY METHODS
KR102851176B1 (ko) * 2018-07-26 2025-08-28 알트리아 클라이언트 서비시즈 엘엘씨 알칼로이드 수준이 변경된 담배 식물 및 제품을 생산하기 위한 pmt 공학에 기초한 조성물 및 방법
US20200035118A1 (en) 2018-07-27 2020-01-30 Joseph Pandolfino Methods and products to facilitate smokers switching to a tobacco heating product or e-cigarettes
US10897925B2 (en) 2018-07-27 2021-01-26 Joseph Pandolfino Articles and formulations for smoking products and vaporizers
US20200031857A1 (en) 2018-07-30 2020-01-30 Dionex Corporation Anion exchange stationary phases based on a polyalkylpolyamine polymer layer

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US6617496B1 (en) * 1985-10-16 2003-09-09 Monsanto Company Effecting virus resistance in plants through the use of negative strand RNAs
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US5260205A (en) * 1990-11-14 1993-11-09 Philip Morris Incorporated Method of purifying putrescine N-methyltransferase from tobacco plant extract with a polyamine

Also Published As

Publication number Publication date
US5369023A (en) 1994-11-29
KR920009843A (ko) 1992-06-25
US5684241A (en) 1997-11-04
EP0486214A2 (en) 1992-05-20
TR27504A (tr) 1995-06-07
EP0486214A3 (en) 1992-11-19
US5260205A (en) 1993-11-09
JPH0584075A (ja) 1993-04-06

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Legal Events

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Ipc: C12N 9/10 (2006.01), A01H 5/12 (2018.0