BRPI0210359B8 - método para medir a polimerização de dna dependente do dna em uma amostra biológica, e, embalagem comercial - Google Patents
método para medir a polimerização de dna dependente do dna em uma amostra biológica, e, embalagem comercialInfo
- Publication number
- BRPI0210359B8 BRPI0210359B8 BRPI0210359A BRPI0210359A BRPI0210359B8 BR PI0210359 B8 BRPI0210359 B8 BR PI0210359B8 BR PI0210359 A BRPI0210359 A BR PI0210359A BR PI0210359 A BRPI0210359 A BR PI0210359A BR PI0210359 B8 BRPI0210359 B8 BR PI0210359B8
- Authority
- BR
- Brazil
- Prior art keywords
- dna
- biological sample
- measuring
- primer
- labeled antibody
- Prior art date
Links
- 239000012472 biological sample Substances 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 230000001419 dependent effect Effects 0.000 title abstract 2
- 238000004806 packaging method and process Methods 0.000 title 1
- 238000006116 polymerization reaction Methods 0.000 title 1
- 102100034343 Integrase Human genes 0.000 abstract 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 2
- 235000011178 triphosphate Nutrition 0.000 abstract 2
- 239000001226 triphosphate Substances 0.000 abstract 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 239000007795 chemical reaction product Substances 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 239000007790 solid phase Substances 0.000 abstract 1
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 abstract 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 abstract 1
- 241001430294 unidentified retrovirus Species 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
método para medir a polimerização de dna dependente do dna em uma amostra biológica, e, embalagem comercial é descrito um método para a medição da polimerização de dna dependente do dna em uma amostra biológica. o método compreende as etapas de fornecer um iniciador com uma seqüência específica curta trançada em filamentos única, o qual é incapaz de pareamento interno de bases, ligadas a uma fase sólida; colocar em contato a construção do iniciador com uma mistura de reação contendo um padrão de deoxinucleotídeo trançado em filamentos único com uma parte da seqüência complementar no iniciador e os quatro trifosfatos de deoxinucleosídeo, um dos quais é modificado de modo que seja especificamente reconhecido por um anticorpo rotulado; adicionar uma amostra biológica que compreende a dna polimerase, tal como a transcriptase reversa (rt) de retrovírus, na mistura; deixar a reação de polimerase prosseguir; incubar o produto da reação imobilizado com o anticorpo rotulado; detectar a quantidade de anticorpo rotulado ligado; e medir a quantidade de trifosfato de deoxinucleosídeo modificado incorporado, como uma medida da polimerização de dna, que pode ser usada em teste da suscetibilidade ao medicamento. uma embalagem comercial é também apresentada.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29777301P | 2001-06-14 | 2001-06-14 | |
| US60/297773 | 2001-06-14 | ||
| PCT/SE2002/001155 WO2002103039A1 (en) | 2001-06-14 | 2002-06-14 | A method for measuring dna polymerization and applications of the method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BR0210359A BR0210359A (pt) | 2004-06-22 |
| BRPI0210359B1 BRPI0210359B1 (pt) | 2020-08-11 |
| BRPI0210359B8 true BRPI0210359B8 (pt) | 2021-07-27 |
Family
ID=23147684
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0210359A BRPI0210359B8 (pt) | 2001-06-14 | 2002-06-14 | método para medir a polimerização de dna dependente do dna em uma amostra biológica, e, embalagem comercial |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US7488577B2 (pt) |
| EP (1) | EP1395675B1 (pt) |
| JP (1) | JP4188228B2 (pt) |
| CN (1) | CN100379879C (pt) |
| AT (1) | ATE316149T1 (pt) |
| AU (1) | AU2002303065B2 (pt) |
| BR (1) | BRPI0210359B8 (pt) |
| DE (1) | DE60208787T2 (pt) |
| ES (1) | ES2257548T3 (pt) |
| WO (1) | WO2002103039A1 (pt) |
| ZA (1) | ZA200309552B (pt) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101275585B1 (ko) | 2005-02-25 | 2013-06-19 | 뢴네르볼 인터내셔널 아베 | 티미딘 키나아제 활성을 결정하는 방법 및 키트 및 그의용도 |
| WO2008024052A1 (en) * | 2006-08-24 | 2008-02-28 | Rönnerbol International Ab | A method and a kit for determination of an enzyme activity involved in metabolic production of a deoxynucleoside triphosphate and use thereof |
| ES3026859T3 (en) | 2017-01-04 | 2025-06-12 | Mgi Tech Co Ltd | Stepwise sequencing by non-labeled reversible terminators or natural nucleotides |
| BR112019027993A2 (pt) * | 2017-07-04 | 2020-07-07 | Cavidi Ab | métodos para avaliar a susceptibilidade de um vírus ao tratamento com um fármaco inibidor de uma enzima do vírus do tipo selvagem, para avaliar se um paciente tratado para uma infecção viral tem necessidade de alterar a terapia farmacológica com um fármaco inibidor da enzima viral e para determinar a carga de um vírus em uma amostra do paciente e a dita resistência do vírus ao tratamento com um fármaco inibidor de uma enzima do vírus do tipo selvagem, e, sistema |
| CN109609606B (zh) * | 2018-11-28 | 2023-03-31 | 成都博奥晶芯生物科技有限公司 | 一种dna聚合酶的相对酶活的测定方法 |
| AU2022279886A1 (en) | 2021-05-25 | 2023-11-02 | Cavidi Ab | Method for sensitive analyte detection assays and kits therefor |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE462497B (sv) * | 1988-11-30 | 1990-07-02 | Clas Fredrik Runesson Kaelland | Saett foer att aktivitetsbestaemma polymeraser |
| SE9902410D0 (sv) * | 1999-06-24 | 1999-06-24 | Cavidi Tech Ab | Reverse transcriptase assay kit, use thereof and method for analysis of RT activity in biological samples |
-
2002
- 2002-06-14 EP EP02731065A patent/EP1395675B1/en not_active Expired - Lifetime
- 2002-06-14 AT AT02731065T patent/ATE316149T1/de not_active IP Right Cessation
- 2002-06-14 BR BRPI0210359A patent/BRPI0210359B8/pt not_active IP Right Cessation
- 2002-06-14 WO PCT/SE2002/001155 patent/WO2002103039A1/en not_active Ceased
- 2002-06-14 AU AU2002303065A patent/AU2002303065B2/en not_active Ceased
- 2002-06-14 DE DE60208787T patent/DE60208787T2/de not_active Expired - Lifetime
- 2002-06-14 US US10/479,510 patent/US7488577B2/en not_active Expired - Lifetime
- 2002-06-14 JP JP2003505360A patent/JP4188228B2/ja not_active Expired - Fee Related
- 2002-06-14 ES ES02731065T patent/ES2257548T3/es not_active Expired - Lifetime
- 2002-06-14 CN CNB028156056A patent/CN100379879C/zh not_active Expired - Fee Related
-
2003
- 2003-12-09 ZA ZA2003/09552A patent/ZA200309552B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN100379879C (zh) | 2008-04-09 |
| ATE316149T1 (de) | 2006-02-15 |
| US7488577B2 (en) | 2009-02-10 |
| JP2004533255A (ja) | 2004-11-04 |
| ZA200309552B (en) | 2005-02-23 |
| AU2002303065B2 (en) | 2007-09-06 |
| BRPI0210359B1 (pt) | 2020-08-11 |
| BR0210359A (pt) | 2004-06-22 |
| EP1395675B1 (en) | 2006-01-18 |
| JP4188228B2 (ja) | 2008-11-26 |
| DE60208787T2 (de) | 2006-11-02 |
| WO2002103039A1 (en) | 2002-12-27 |
| US20040157230A1 (en) | 2004-08-12 |
| DE60208787D1 (de) | 2006-04-06 |
| CN1541276A (zh) | 2004-10-27 |
| EP1395675A1 (en) | 2004-03-10 |
| ES2257548T3 (es) | 2006-08-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] | ||
| B07G | Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 11/08/2020, OBSERVADAS AS CONDICOES LEGAIS. |
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| B21F | Lapse acc. art. 78, item iv - on non-payment of the annual fees in time |
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| B16C | Correction of notification of the grant [chapter 16.3 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 14/06/2002, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO |
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| B24J | Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12) |
Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2624 DE 20-04-2021 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |