BRPI0403221A - Fragmento de taq polimerase termoestável - Google Patents

Fragmento de taq polimerase termoestável

Info

Publication number
BRPI0403221A
BRPI0403221A BR0403221-7A BRPI0403221A BRPI0403221A BR PI0403221 A BRPI0403221 A BR PI0403221A BR PI0403221 A BRPI0403221 A BR PI0403221A BR PI0403221 A BRPI0403221 A BR PI0403221A
Authority
BR
Brazil
Prior art keywords
delta
taq
taqwt
nucleic acid
well
Prior art date
Application number
BR0403221-7A
Other languages
English (en)
Inventor
Waltraud Ankenbauer
Thomas Meier
Annette Deufel
Dieter Heindl
Gisela Betzl
Rainer Schmuck
Bernd Schneidinger
Jessica Strey
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BRPI0403221A publication Critical patent/BRPI0403221A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

"FRAGMENTO DE TAQ POLIMERASE TERMOESTáVEL". A presente invenção refere-se a um fragmento de DNA polimerase Thermus aquaticus (TaqWT) desprovido de aminoácidos N-terminais 288 (Taq<30>288) possui uma termostabilidade aumentada sobre as TaqWT, Taq<30>279, e Taq<30>289. A presente invenção portanto fornece Taq<30>288, os vetores de expressão recombinantes que codificam a mesma ou seus derivados, assim como os protocolos de purificação para a Taq<30>288. A invenção também abrange kits contendo Taq<30>288 assim como o uso de Taq<30>288 e kits contendo Taq<30>288. Além disso, a invenção abrange métodos para o seq³enciamento de um padrão de ácido nucléico e métodos para a amplificação de um ácido nucléico alvo.
BR0403221-7A 2003-08-12 2004-08-12 Fragmento de taq polimerase termoestável BRPI0403221A (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03017636 2003-08-12

Publications (1)

Publication Number Publication Date
BRPI0403221A true BRPI0403221A (pt) 2005-07-12

Family

ID=34130067

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0403221-7A BRPI0403221A (pt) 2003-08-12 2004-08-12 Fragmento de taq polimerase termoestável

Country Status (14)

Country Link
US (1) US7972830B2 (pt)
EP (1) EP1507002B1 (pt)
JP (1) JP3929061B2 (pt)
KR (1) KR100626578B1 (pt)
CN (1) CN1590543B (pt)
AT (1) ATE348881T1 (pt)
AU (1) AU2004203649B2 (pt)
BR (1) BRPI0403221A (pt)
CA (1) CA2474734C (pt)
DE (1) DE602004003755T2 (pt)
DK (1) DK1507002T3 (pt)
ES (1) ES2278249T3 (pt)
MX (1) MXPA04007721A (pt)
SG (1) SG109552A1 (pt)

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US20140255925A9 (en) * 2008-04-30 2014-09-11 Integrated Dna Technologies Modified rnase h enzymes and their uses
US9434988B2 (en) 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
DK2644707T3 (en) 2008-04-30 2015-06-29 Integrated Dna Tech Inc RNase-H-based assays using modified RNA monomers.
US10457968B2 (en) 2008-11-03 2019-10-29 Kapa Biosystems, Inc. Modified type A DNA polymerases
CN102858962A (zh) * 2009-11-19 2013-01-02 索利斯拜欧代因公司 用于增加多肽稳定性和活性的组合物及相关方法
US20110250598A1 (en) 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
CN106518696B (zh) 2011-06-08 2019-06-14 生命技术公司 用于pcr系统的新型去污剂的设计和开发
EP3063129B1 (en) 2013-10-25 2019-04-17 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
EP4461821A3 (en) * 2014-12-16 2025-04-09 Life Technologies Corporation Polymerase compositions and methods of making and using same
WO2016193070A1 (en) 2015-05-29 2016-12-08 Roche Diagnostics Gmbh A method of inactivating microbes by citraconic anhydride
CN108473970B (zh) * 2015-11-27 2022-09-13 国立大学法人九州大学 Dna聚合酶变体
GB201604876D0 (en) * 2016-03-22 2016-05-04 Uni I Tromsø Norges Arktiske Uni Marine DNA polymerases
CN108265039B (zh) * 2016-12-30 2020-07-14 天津强微特生物科技有限公司 一种突变TaqDNA聚合酶及其纯化方法
WO2018183612A1 (en) * 2017-03-29 2018-10-04 Editas Medicine, Inc. Nucleic acid mutagenesis methods
US11299776B2 (en) * 2017-05-10 2022-04-12 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
EP3740591A4 (en) * 2018-01-19 2022-02-23 Bio-Rad Laboratories, Inc. Mutant dna polymerases
GB201812428D0 (en) * 2018-07-31 2018-09-12 Bg Res Ltd Improved method for pathogen detection
CA3175648A1 (en) 2020-04-30 2021-11-04 Alcino Orfeu De Leao E Flores Method and portable device for detection of nucleic sequences in suspected coronavirus samples
WO2022003565A1 (en) * 2020-07-01 2022-01-06 Medimmune, Llc Detergent and method for purifying a biotherapeutic
US11649441B2 (en) * 2020-10-07 2023-05-16 Abclonal Science, Inc. Taq DNA polymerase mutants for probe qPCR
WO2023122291A2 (en) * 2021-12-23 2023-06-29 Sherlock Biosciences, Inc. Methods for polypeptide purification
CN116024320A (zh) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 一种用于检测核酸的荧光定量pcr方法

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NZ199722A (en) 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
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Also Published As

Publication number Publication date
JP2005058236A (ja) 2005-03-10
DK1507002T3 (da) 2007-04-10
KR100626578B1 (ko) 2006-09-25
ES2278249T3 (es) 2007-08-01
AU2004203649A1 (en) 2005-03-03
CN1590543A (zh) 2005-03-09
EP1507002A2 (en) 2005-02-16
EP1507002A3 (en) 2005-07-27
US7972830B2 (en) 2011-07-05
MXPA04007721A (es) 2005-07-12
CN1590543B (zh) 2010-05-12
US20050037412A1 (en) 2005-02-17
CA2474734C (en) 2010-07-13
JP3929061B2 (ja) 2007-06-13
SG109552A1 (en) 2005-03-30
CA2474734A1 (en) 2005-02-12
KR20050025266A (ko) 2005-03-14
ATE348881T1 (de) 2007-01-15
AU2004203649B2 (en) 2006-01-12
EP1507002B1 (en) 2006-12-20
DE602004003755T2 (de) 2007-10-11
HK1074458A1 (zh) 2005-11-11
DE602004003755D1 (de) 2007-02-01

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O, 11, 13, 10, 18 DA LPI

B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.