BRPI0403221A - Fragmento de taq polimerase termoestável - Google Patents

Fragmento de taq polimerase termoestável

Info

Publication number
BRPI0403221A
BRPI0403221A BR0403221-7A BRPI0403221A BRPI0403221A BR PI0403221 A BRPI0403221 A BR PI0403221A BR PI0403221 A BRPI0403221 A BR PI0403221A BR PI0403221 A BRPI0403221 A BR PI0403221A
Authority
BR
Brazil
Prior art keywords
delta
taq
taqwt
nucleic acid
well
Prior art date
Application number
BR0403221-7A
Other languages
English (en)
Inventor
Waltraud Ankenbauer
Thomas Meier
Annette Deufel
Dieter Heindl
Gisela Betzl
Rainer Schmuck
Bernd Schneidinger
Jessica Strey
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BRPI0403221A publication Critical patent/BRPI0403221A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

"FRAGMENTO DE TAQ POLIMERASE TERMOESTáVEL". A presente invenção refere-se a um fragmento de DNA polimerase Thermus aquaticus (TaqWT) desprovido de aminoácidos N-terminais 288 (Taq<30>288) possui uma termostabilidade aumentada sobre as TaqWT, Taq<30>279, e Taq<30>289. A presente invenção portanto fornece Taq<30>288, os vetores de expressão recombinantes que codificam a mesma ou seus derivados, assim como os protocolos de purificação para a Taq<30>288. A invenção também abrange kits contendo Taq<30>288 assim como o uso de Taq<30>288 e kits contendo Taq<30>288. Além disso, a invenção abrange métodos para o seq³enciamento de um padrão de ácido nucléico e métodos para a amplificação de um ácido nucléico alvo.
BR0403221-7A 2003-08-12 2004-08-12 Fragmento de taq polimerase termoestável BRPI0403221A (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03017636 2003-08-12

Publications (1)

Publication Number Publication Date
BRPI0403221A true BRPI0403221A (pt) 2005-07-12

Family

ID=34130067

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0403221-7A BRPI0403221A (pt) 2003-08-12 2004-08-12 Fragmento de taq polimerase termoestável

Country Status (14)

Country Link
US (1) US7972830B2 (pt)
EP (1) EP1507002B1 (pt)
JP (1) JP3929061B2 (pt)
KR (1) KR100626578B1 (pt)
CN (1) CN1590543B (pt)
AT (1) ATE348881T1 (pt)
AU (1) AU2004203649B2 (pt)
BR (1) BRPI0403221A (pt)
CA (1) CA2474734C (pt)
DE (1) DE602004003755T2 (pt)
DK (1) DK1507002T3 (pt)
ES (1) ES2278249T3 (pt)
MX (1) MXPA04007721A (pt)
SG (1) SG109552A1 (pt)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006074217A2 (en) * 2005-01-04 2006-07-13 Stratagene California Hot start polymerase reaction using a thermolabile blocker
DE102006025154A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität
EP2113574A1 (en) 2008-04-28 2009-11-04 Biotype AG Substances and methods for a DNA based profiling assay
US20140255925A9 (en) * 2008-04-30 2014-09-11 Integrated Dna Technologies Modified rnase h enzymes and their uses
EP2644710B1 (en) 2008-04-30 2016-12-21 Integrated Dna Technologies, Inc. Rnase-h-based assays utilizing modified rna monomers
WO2010062777A2 (en) * 2008-11-03 2010-06-03 Kapabiosystems Modified type a dna polymerases
IN2012DN05145A (pt) * 2009-11-19 2015-10-23 Solis Biodyne
US20110250598A1 (en) 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
US9315787B2 (en) 2011-01-14 2016-04-19 Kapa Biosystems, Inc. Modified DNA polymerases for improved amplification
AU2012236896A1 (en) 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
DK2718260T3 (en) 2011-06-08 2018-11-19 Life Technologies Corp DESIGN AND DEVELOPMENT OF NEW DETERGENTS FOR USE IN PCR SYSTEMS
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
CN106458885B (zh) 2013-10-25 2019-12-10 生命技术公司 用于聚合酶链式反应系统的新颖化合物及其应用
EP3795681B1 (en) * 2014-12-16 2024-09-18 Life Technologies Corporation Polymerase compositions and methods of making and using same
JP6822974B2 (ja) 2015-05-29 2021-01-27 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ シトラコン酸無水物によって微生物を不活性化する方法
US11046939B2 (en) * 2015-11-27 2021-06-29 Kyushu University, National University Corporation DNA polymerase variant
GB201604876D0 (en) * 2016-03-22 2016-05-04 Uni I Tromsø Norges Arktiske Uni Marine DNA polymerases
CN108265039B (zh) * 2016-12-30 2020-07-14 天津强微特生物科技有限公司 一种突变TaqDNA聚合酶及其纯化方法
WO2018183612A1 (en) * 2017-03-29 2018-10-04 Editas Medicine, Inc. Nucleic acid mutagenesis methods
WO2018209092A1 (en) * 2017-05-10 2018-11-15 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
WO2019143622A1 (en) * 2018-01-19 2019-07-25 Bio-Rad Laboratories, Inc. Mutant dna polymerases
GB201812428D0 (en) * 2018-07-31 2018-09-12 Bg Res Ltd Improved method for pathogen detection
BR112022021086A2 (pt) 2020-04-30 2022-12-13 Stab Vida Investig E Servicos Em Ciencias Biologicas Lda Sistema integrado para a detecção e identificação de sequências específicas de ácidos nucleicos e método de utilização do sistema integrado
CN115996940A (zh) * 2020-07-01 2023-04-21 免疫医疗有限责任公司 用于纯化生物治疗剂的洗涤剂和方法
US11649441B2 (en) * 2020-10-07 2023-05-16 Abclonal Science, Inc. Taq DNA polymerase mutants for probe qPCR
WO2023122291A2 (en) * 2021-12-23 2023-06-29 Sherlock Biosciences, Inc. Methods for polypeptide purification
CN116024320A (zh) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 一种用于检测核酸的荧光定量pcr方法
CN121628873A (zh) * 2026-02-04 2026-03-10 中科院南昌高新技术产业协同创新研究院 一种双抗体封闭Taq DNA聚合酶及其构建方法与应用

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
NZ199722A (en) 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
AU606043B2 (en) 1985-03-28 1991-01-31 F. Hoffmann-La Roche Ag Detection of viruses by amplification and hybridization
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA1284931C (en) 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5108892A (en) 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
FR2652520B1 (fr) 1989-10-02 1992-02-07 Sgn Soc Gen Tech Nouvelle Procede et dispositif pour maintenir une atmosphere propre a temperature regulee sur un poste de travail.
AU6735490A (en) 1989-10-16 1991-05-16 E.I. Du Pont De Nemours And Company Process for chlorofluoropropanes
EP0894860B1 (en) * 1990-09-28 2012-12-12 F. Hoffmann-La Roche AG Thermostable DNA polymerases and deletion mutants thereof
AU8906091A (en) 1990-10-05 1992-04-28 Wayne M. Barnes Thermostable dna polymerase
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
CA2222744C (en) 1995-05-31 2008-03-25 Amersham Life Science, Inc. Thermostable dna polymerases
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
US6130045A (en) * 1998-06-11 2000-10-10 Clontech Laboratories, Inc. Thermostable polymerase
DK1069131T3 (da) 1999-07-15 2006-06-26 Qiagen Gmbh Fremgangsmåde til at skille partikelformige substrater fra en oplösning med et minimalt tab af partikler
GB9920194D0 (en) 1999-08-27 1999-10-27 Advanced Biotech Ltd A heat-stable thermostable DNA polymerase for use in nucleic acid amplification
JP4768155B2 (ja) 2001-07-02 2011-09-07 独立行政法人理化学研究所 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法
CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro

Also Published As

Publication number Publication date
HK1074458A1 (zh) 2005-11-11
EP1507002B1 (en) 2006-12-20
CA2474734A1 (en) 2005-02-12
JP3929061B2 (ja) 2007-06-13
EP1507002A3 (en) 2005-07-27
DE602004003755D1 (de) 2007-02-01
EP1507002A2 (en) 2005-02-16
US7972830B2 (en) 2011-07-05
AU2004203649A1 (en) 2005-03-03
CA2474734C (en) 2010-07-13
KR100626578B1 (ko) 2006-09-25
JP2005058236A (ja) 2005-03-10
CN1590543A (zh) 2005-03-09
AU2004203649B2 (en) 2006-01-12
ES2278249T3 (es) 2007-08-01
US20050037412A1 (en) 2005-02-17
KR20050025266A (ko) 2005-03-14
SG109552A1 (en) 2005-03-30
DK1507002T3 (da) 2007-04-10
DE602004003755T2 (de) 2007-10-11
MXPA04007721A (es) 2005-07-12
ATE348881T1 (de) 2007-01-15
CN1590543B (zh) 2010-05-12

Similar Documents

Publication Publication Date Title
ATE348881T1 (de) Thermostabiles taq polymerase fragment
EP2272952B8 (en) Thermostable or thermoactive DNA polymerase with attenuated 3&#39;-5&#39; exonuclease activity
ATE459710T1 (de) Dna-polymerasefusionen und deren verwendungen
DK0624641T3 (da) Termostabil nukleinsyrepolymerase
DK0550687T3 (da) 5&#39; til 3&#39;-exonukleasemutationer af termostabile DNA-polymeraser
ATE325877T1 (de) Mutierte chimäre dns-polymerasen
DE60110564D1 (en) Rna polymerase mutanten mit erhöhter thermostabilität
DE60301325D1 (de) Mutation von dna polymerasen aus archaeobakterien
ATE371022T1 (de) Fy7 polymerase
DE60332781D1 (de) Verfahren und Zusammensetzungen zur Identifizierung und Charakterisierung von Hepatitis C
EA200300246A1 (ru) Ген и белок, ассоциированные с шизофренией
ATE496122T1 (de) Thermostabile polymerase aus i thermococcus pacificus /i
Ikeuchi et al. The enhancing of a cysteine proteinase activity at acidic pH by protein engineering, the role of glutamic 50 in the enzyme mechanism of caricain
IL195194A0 (en) Thermostable dna polymerase of the archaeal ampullavirus abv and its applications
DE602005023586D1 (de) Primer- und sondenkonstruktion zur effizienten amplifikation und detektion des 3&#39;-nichttranslatierten bereichs von hcv
DK2186898T3 (da) Blomstrings-induktion
EP1312677A4 (en) NUCLEIC ACIDS ENCODING PROTEINS OF LETTUCE VEGETABLE HYPERTROPHY VIRUSES AND USE THEREOF
AR040993A1 (es) Procedimiento para la identificacion de sustancias con accion herbicida
DK1789541T3 (da) Overeksprimeret og renset Aspergillus ficuum-oxidase og nukleinsyre, der koder derfor
WO2002022823A3 (de) An der stressantwort beteiligte proteine und dafür codierende gene aus ashbya gossypii
WO2005049797A3 (en) Cynomolgus monkey dickkopf-4, nucleotides encoding same, and uses thereof
DE602006005932D1 (de) Verfahren zur identifizierung von proteinfaltungsinhibitoren

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O, 11, 13, 10, 18 DA LPI

B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.