DK1507002T3 - Termostabilt Taq-polymerasefragment - Google Patents

Termostabilt Taq-polymerasefragment

Info

Publication number
DK1507002T3
DK1507002T3 DK04018913T DK04018913T DK1507002T3 DK 1507002 T3 DK1507002 T3 DK 1507002T3 DK 04018913 T DK04018913 T DK 04018913T DK 04018913 T DK04018913 T DK 04018913T DK 1507002 T3 DK1507002 T3 DK 1507002T3
Authority
DK
Denmark
Prior art keywords
delta
taq
taqwt
nucleic acid
well
Prior art date
Application number
DK04018913T
Other languages
Danish (da)
English (en)
Inventor
Waltraud Dr Ankenbauer
Thomas Dr Meier
Annette Dr Deufel
Dieter Dr Heindl
Gisela Dr Betzl
Rainer Dr Schmuck
Bernd Dr Schneidinger
Jessica Strey
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK1507002T3 publication Critical patent/DK1507002T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK04018913T 2003-08-12 2004-08-10 Termostabilt Taq-polymerasefragment DK1507002T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03017636 2003-08-12

Publications (1)

Publication Number Publication Date
DK1507002T3 true DK1507002T3 (da) 2007-04-10

Family

ID=34130067

Family Applications (1)

Application Number Title Priority Date Filing Date
DK04018913T DK1507002T3 (da) 2003-08-12 2004-08-10 Termostabilt Taq-polymerasefragment

Country Status (14)

Country Link
US (1) US7972830B2 (pt)
EP (1) EP1507002B1 (pt)
JP (1) JP3929061B2 (pt)
KR (1) KR100626578B1 (pt)
CN (1) CN1590543B (pt)
AT (1) ATE348881T1 (pt)
AU (1) AU2004203649B2 (pt)
BR (1) BRPI0403221A (pt)
CA (1) CA2474734C (pt)
DE (1) DE602004003755T2 (pt)
DK (1) DK1507002T3 (pt)
ES (1) ES2278249T3 (pt)
MX (1) MXPA04007721A (pt)
SG (1) SG109552A1 (pt)

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DE102006025154A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität
EP2113574A1 (en) 2008-04-28 2009-11-04 Biotype AG Substances and methods for a DNA based profiling assay
US20140255925A9 (en) * 2008-04-30 2014-09-11 Integrated Dna Technologies Modified rnase h enzymes and their uses
US9434988B2 (en) 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
DK2279263T3 (da) 2008-04-30 2013-11-11 Integrated Dna Tech Inc Rnase-h-baserede assays med anvendelse af modificerede rna-monomerer
CA2742594C (en) 2008-11-03 2020-03-24 Kapa Biosystems, Inc. Modified type a dna polymerases
IN2012DN05145A (pt) * 2009-11-19 2015-10-23 Solis Biodyne
US20110250598A1 (en) 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
FI3461807T3 (fi) 2011-06-08 2023-09-07 Life Technologies Corp Uudenlaisten detergenttien suunnittelu ja kehitys pcr-järjestelmissä käyttöä varten
EP3539944A1 (en) 2013-10-25 2019-09-18 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
CN113403293B (zh) * 2014-12-16 2024-08-16 生命技术公司 聚合酶组合物和制造与使用其的方法
JP6822974B2 (ja) 2015-05-29 2021-01-27 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ シトラコン酸無水物によって微生物を不活性化する方法
US11046939B2 (en) * 2015-11-27 2021-06-29 Kyushu University, National University Corporation DNA polymerase variant
GB201604876D0 (en) * 2016-03-22 2016-05-04 Uni I Tromsø Norges Arktiske Uni Marine DNA polymerases
CN108265039B (zh) * 2016-12-30 2020-07-14 天津强微特生物科技有限公司 一种突变TaqDNA聚合酶及其纯化方法
WO2018183612A1 (en) * 2017-03-29 2018-10-04 Editas Medicine, Inc. Nucleic acid mutagenesis methods
WO2018209092A1 (en) * 2017-05-10 2018-11-15 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
US10961518B2 (en) 2018-01-19 2021-03-30 Bio-Rad Laboratories, Inc. Mutant DNA polymerases
GB201812428D0 (en) * 2018-07-31 2018-09-12 Bg Res Ltd Improved method for pathogen detection
JP2023524117A (ja) 2020-04-30 2023-06-08 スタブ ヴィーダ － インヴェスティガサォン エ セルヴィソス エム シエンシアス バイオロジカス エルディーエー コロナウイルスの疑いのある試料中の核酸配列を検出するための方法及びポータブルデバイス
CN115996940A (zh) * 2020-07-01 2023-04-21 免疫医疗有限责任公司 用于纯化生物治疗剂的洗涤剂和方法
US11649441B2 (en) * 2020-10-07 2023-05-16 Abclonal Science, Inc. Taq DNA polymerase mutants for probe qPCR
WO2023122291A2 (en) * 2021-12-23 2023-06-29 Sherlock Biosciences, Inc. Methods for polypeptide purification
CN116024320A (zh) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 一种用于检测核酸的荧光定量pcr方法

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US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
NZ199722A (en) 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
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WO1991005753A1 (en) 1989-10-16 1991-05-02 E.I. Du Pont De Nemours And Company Process for chlorofluoropropanes
DE553264T1 (de) 1990-10-05 1994-04-28 Wayne M Barnes Thermostabile dna polymerase.
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5885813A (en) 1995-05-31 1999-03-23 Amersham Life Science, Inc. Thermostable DNA polymerases
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
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CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro

Also Published As

Publication number Publication date
CA2474734A1 (en) 2005-02-12
CN1590543B (zh) 2010-05-12
EP1507002A2 (en) 2005-02-16
KR100626578B1 (ko) 2006-09-25
US20050037412A1 (en) 2005-02-17
EP1507002B1 (en) 2006-12-20
US7972830B2 (en) 2011-07-05
DE602004003755T2 (de) 2007-10-11
AU2004203649A1 (en) 2005-03-03
SG109552A1 (en) 2005-03-30
ES2278249T3 (es) 2007-08-01
MXPA04007721A (es) 2005-07-12
CN1590543A (zh) 2005-03-09
JP2005058236A (ja) 2005-03-10
AU2004203649B2 (en) 2006-01-12
CA2474734C (en) 2010-07-13
BRPI0403221A (pt) 2005-07-12
KR20050025266A (ko) 2005-03-14
DE602004003755D1 (de) 2007-02-01
ATE348881T1 (de) 2007-01-15
HK1074458A1 (zh) 2005-11-11
EP1507002A3 (en) 2005-07-27
JP3929061B2 (ja) 2007-06-13

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