ATE325877T1 - Mutierte chimäre dns-polymerasen - Google Patents

Mutierte chimäre dns-polymerasen

Info

Publication number
ATE325877T1
ATE325877T1 AT98112327T AT98112327T ATE325877T1 AT E325877 T1 ATE325877 T1 AT E325877T1 AT 98112327 T AT98112327 T AT 98112327T AT 98112327 T AT98112327 T AT 98112327T AT E325877 T1 ATE325877 T1 AT E325877T1
Authority
AT
Austria
Prior art keywords
dna polymerase
enzymes
thermostable dna
chimeric thermostable
mutant
Prior art date
Application number
AT98112327T
Other languages
English (en)
Inventor
David Harrow Gelfand
Fred Lawrence Reichert
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE325877T1 publication Critical patent/ATE325877T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT98112327T 1997-07-09 1998-07-03 Mutierte chimäre dns-polymerasen ATE325877T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US5206597P 1997-07-09 1997-07-09

Publications (1)

Publication Number Publication Date
ATE325877T1 true ATE325877T1 (de) 2006-06-15

Family

ID=21975226

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98112327T ATE325877T1 (de) 1997-07-09 1998-07-03 Mutierte chimäre dns-polymerasen

Country Status (7)

Country Link
US (1) US6228628B1 (de)
EP (1) EP0892058B1 (de)
JP (1) JP4350815B2 (de)
AT (1) ATE325877T1 (de)
CA (1) CA2240570C (de)
DE (1) DE69836779T2 (de)
ES (1) ES2263188T3 (de)

Families Citing this family (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6759226B1 (en) * 2000-05-24 2004-07-06 Third Wave Technologies, Inc. Enzymes for the detection of specific nucleic acid sequences
EP1007730A4 (de) 1996-08-30 2004-04-28 Invitrogen Corp Verfahren zur identifizierung und isolierung von spezifischen nukleotidsequenzen in cdna und genomischer dna
US6306588B1 (en) * 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
DE19810879A1 (de) 1998-03-13 1999-09-16 Roche Diagnostics Gmbh Polymerasenchimären
GB9818432D0 (en) * 1998-08-24 1998-10-21 Bioline Limited Thermostable DNA Polymerase
EP1259624B1 (de) 2000-02-17 2008-05-14 Qiagen GmbH Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen
JP4989004B2 (ja) * 2000-02-23 2012-08-01 シティ・オブ・ホープ 加ピロリン酸分解活性化重合（ｐａｐ）：アリル−特異的増幅および核酸配列決定への適応
US7033763B2 (en) 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
CA2410072C (en) * 2000-05-24 2011-07-19 Third Wave Technologies, Inc. Polymerase cleavage agents and detection of rna
US6627424B1 (en) * 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
WO2002004022A1 (en) * 2000-07-12 2002-01-17 Invitrogen Corp High fidelity polymerases and uses thereof
US7238505B2 (en) 2000-10-04 2007-07-03 Ahram Biosystems Inc. Immobilized DNA polymerase
DE10049211A1 (de) * 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
CA2426540A1 (en) * 2000-10-20 2002-07-25 Biocardia, Inc. Leukocyte expression profiling
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) * 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7026121B1 (en) 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US20050042629A1 (en) * 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
WO2003046149A2 (en) * 2001-11-28 2003-06-05 Mj Bioworks Incorporated Methods of using improved polymerases
AU2002346498A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases
US7148049B2 (en) * 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
US7666645B2 (en) * 2002-10-23 2010-02-23 Bio-Rad Laboratories, Inc. Sso7-polymerase conjugate proteins
US7960157B2 (en) * 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
US7892745B2 (en) * 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
GB2416538B (en) * 2004-07-26 2008-07-30 Bioline Ltd Chimeric DNA polymerase
WO2006029184A2 (en) * 2004-09-08 2006-03-16 Expression Diagnostics, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US20060223067A1 (en) * 2005-03-31 2006-10-05 Paolo Vatta Mutant DNA polymerases and methods of use
US7378260B2 (en) * 2005-04-01 2008-05-27 Applera Corporation Products and methods for reducing dye artifacts
EP1885889A4 (de) * 2005-05-11 2010-01-20 Expression Diagnostics Inc Verfahren zur überwachung des betriebsstatus von transplantaten über genlisten
US20060292578A1 (en) 2005-06-28 2006-12-28 Weidong Zheng DNA polymerase blends and mutant DNA polymerases
WO2007016702A2 (en) * 2005-07-29 2007-02-08 Applera Corporation Chimeric polymerases
EP2007909A4 (de) * 2006-04-07 2011-01-26 Xdx Inc Steroidreaktive nukleinsäureexpression und voraussage von erkrankungsaktivitäten
WO2008021431A2 (en) 2006-08-14 2008-02-21 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
EP2102367A2 (de) * 2006-11-09 2009-09-23 XDX, Inc. Verfahren zur diagnose und überwachung des status von systemischem lupus erythematodes
US8540517B2 (en) * 2006-11-27 2013-09-24 Pharos Innovations, Llc Calculating a behavioral path based on a statistical profile
ES2553893T3 (es) * 2007-11-28 2015-12-14 F. Hoffmann-La Roche Ag ADN polimerasas mutantes con capacidad mejorada de polimerización activada por pirofosforolisis (PAP)
WO2010062777A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Modified type a dna polymerases
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
US9428737B1 (en) * 2010-01-11 2016-08-30 Eric Gaucher Taq polymerase sequences useful for incorporating analogs of nucleoside triphosphates
KR101622442B1 (ko) 2010-06-18 2016-05-19 에프. 호프만-라 로슈 아게 ３’―미스매치 구별이 증가된 ｄｎａ 중합효소
EP2582804B1 (de) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen
US8722378B2 (en) 2010-06-18 2014-05-13 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
WO2011157430A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
WO2011157435A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
WO2011157438A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increasesed 3'-mismatch discrimination
CA2802000C (en) 2010-06-18 2016-08-16 F. Hoffmann-La Roche Ag Dna polymerases with increased 3'-mismatch discrimination
JP5876477B2 (ja) 2010-06-18 2016-03-02 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ
WO2012082753A2 (en) 2010-12-13 2012-06-21 Life Technologies Corporation Polymerization of nucleic acids using activation by polyphosphorolysis (app) reactions
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
EP2675897B1 (de) 2011-02-15 2016-05-11 Roche Diagnostics GmbH Dna-polymerasen mit erhöhter unterscheidung von 3'-diskrepanz
CA2831180C (en) 2011-04-11 2017-02-14 Keith Bauer Dna polymerases with improved activity
EP4249603A3 (de) 2011-06-08 2024-01-03 Life Technologies Corporation Design und entwicklung neuartiger reinigungsmittel zur verwendung in pcr-systemen
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
WO2013013822A1 (en) 2011-07-28 2013-01-31 Roche Diagnostics Gmbh Dna polymerases with improved activity
EP2788479B1 (de) 2011-12-08 2016-02-10 Roche Diagnostics GmbH Dna-polymerasen mit verbesserter aktivität
CN103998603B (zh) 2011-12-08 2016-05-25 霍夫曼-拉罗奇有限公司 具有改进活性的dna聚合酶
JP6144697B2 (ja) 2011-12-08 2017-06-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
WO2014071322A1 (en) 2012-11-02 2014-05-08 Life Technologies Corporation Small RNA Capture, Detection and Quantification
US11254977B2 (en) 2013-03-12 2022-02-22 Life Technologies Corporation Universal reporter-based genotyping methods and materials
US9914964B2 (en) 2013-10-25 2018-03-13 Life Technologies Corporation Compounds for use in PCR systems and applications thereof
PL3161154T3 (pl) * 2014-06-27 2020-10-19 Illumina, Inc. Zmodyfikowane polimerazy do ulepszonego włączania analogów nukleotydów
EP3268491B1 (de) 2015-03-13 2021-07-14 Life Technologies Corporation Verfahren, zusammensetzungen und kits für erfassung, nachweis und quantifizierung von kleinen rna-molekülen
WO2019122200A1 (en) 2017-12-22 2019-06-27 Thermo Fisher Scientific Baltics Uab Polymerase chain reaction composition comprising amines
JP7319990B2 (ja) 2018-03-21 2023-08-02 エフ． ホフマン－ラ ロシュ アーゲー メチル化ｄＮＴＰを効率的かつ効果的に組み込むＤＮＡポリメラーゼ
US11739306B2 (en) 2018-09-13 2023-08-29 Roche Molecular Systems, Inc. Mutant DNA polymerase(s) with improved strand displacement ability
MY203805A (en) 2018-10-31 2024-07-19 Illumina Inc Polymerases, compositions, and methods of use
MX2020013357A (es) 2018-12-05 2021-04-28 Illumina Inc Polimerasas, composiciones y metodos de uso.
JP7209980B2 (ja) * 2020-12-11 2023-01-23 東洋紡株式会社 Ｄｎａポリメラーゼの５’→３’エキソヌクレアーゼ活性ドメインに特異的に結合する抗体
US12077789B2 (en) 2021-08-14 2024-09-03 Illumina, Inc. Polymerases, compositions, and methods of use
CN115772533B (zh) * 2022-08-24 2025-06-06 江南大学 一种细菌连续进化系统、正交易错dna聚合酶及连续进化方法
CN118460500B (zh) * 2024-04-28 2025-01-07 湖南艾科瑞生物工程有限公司 一种融合dna聚合酶及其制备方法与应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
JP2709311B2 (ja) * 1990-09-28 1998-02-04 エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異
US5474920A (en) 1993-11-23 1995-12-12 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University Modified thermo-resistant DNA polymerases
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
ES2072238T3 (es) * 1994-10-17 1997-01-16 Harvard College Adn-polimerasas que tienen un sitio de union con nucleotidos modificados para la secuenciacion de adn.
US5614365A (en) 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
WO1997009451A1 (en) * 1995-09-08 1997-03-13 Life Technologies, Inc. Cloned dna polymerases from thermotoga and mutants thereof

Also Published As

Publication number Publication date
EP0892058A2 (de) 1999-01-20
JP4350815B2 (ja) 2009-10-21
EP0892058A3 (de) 2001-09-26
DE69836779D1 (de) 2007-02-15
CA2240570A1 (en) 1999-01-09
US6228628B1 (en) 2001-05-08
DE69836779T2 (de) 2007-04-26
EP0892058B1 (de) 2006-05-10
CA2240570C (en) 2009-12-15
JPH1189588A (ja) 1999-04-06
ES2263188T3 (es) 2006-12-01

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