BRPI0412535A - serina acetiltransferase mutante, dna, bactéria, e, método para produzir l-cisteìna - Google Patents

serina acetiltransferase mutante, dna, bactéria, e, método para produzir l-cisteìna

Info

Publication number
BRPI0412535A
BRPI0412535A BRPI0412535-5A BRPI0412535A BRPI0412535A BR PI0412535 A BRPI0412535 A BR PI0412535A BR PI0412535 A BRPI0412535 A BR PI0412535A BR PI0412535 A BRPI0412535 A BR PI0412535A
Authority
BR
Brazil
Prior art keywords
cysteine
bacterium
dna
producing
serine acetyltransferase
Prior art date
Application number
BRPI0412535-5A
Other languages
English (en)
Inventor
Tatsuki Kashiwagi
Yuko Kai
Kohki Ishikawa
Ei-Ichiro Suzuki
Hiroshi Takagi
Mikhail Kharisovich Ziyatdinov
Ekaterina Igorevna Redkina
Mikhail Markovich Gusyatiner
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from RU2003121601/13A external-priority patent/RU2003121601A/ru
Priority claimed from RU2003135291/13A external-priority patent/RU2279477C2/ru
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Publication of BRPI0412535A publication Critical patent/BRPI0412535A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"SERINA ACETIlTRANSFERASE MUTANTE, DNA, BACTéRIA, E, MéTODO PARA PRODUZIR L-CISTEìNA". O-acetilserina, L-cisteína e compostos sulfurosos delas derivados, são produzidos mediante o cultivo de bactérias do gênero Escherichia retendo uma acetiltransferase variante que é uma serina acetiltransferase do tipo selvagem tendo a seqüência de aminoácidos correspondente às 89<a> a 96<a> posições substituídas por qualquer uma das seqüências de aminoácidos de SEQ ID NOS: 4 a 9 e tendo a retroinibição pela dessensibilização da L-cisteína.
BRPI0412535-5A 2003-07-16 2004-07-16 serina acetiltransferase mutante, dna, bactéria, e, método para produzir l-cisteìna BRPI0412535A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
RU2003121601/13A RU2003121601A (ru) 2003-07-16 2003-07-16 Мутантная серинацетилтрансфераза
RU2003135291/13A RU2279477C2 (ru) 2003-12-05 2003-12-05 Мутантная серинацетилтрансфераза, фрагмент днк, кодирующий мутантную серинацетилтрансферазу (варианты), бактерия, принадлежащая к роду escherichia, - продуцент l-цистеина, и способ продукции l-цистеина
PCT/JP2004/010216 WO2005007841A1 (ja) 2003-07-16 2004-07-16 変異型セリンアセチルトランスフェラーゼ及びｌ−システインの製造法

Publications (1)

Publication Number Publication Date
BRPI0412535A true BRPI0412535A (pt) 2006-09-19

Family

ID=34082460

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0412535-5A BRPI0412535A (pt) 2003-07-16 2004-07-16 serina acetiltransferase mutante, dna, bactéria, e, método para produzir l-cisteìna

Country Status (6)

Country Link
US (1) US7312058B2 (pt)
EP (1) EP1650296B1 (pt)
JP (1) JP4923573B2 (pt)
AT (1) ATE554162T1 (pt)
BR (1) BRPI0412535A (pt)
WO (1) WO2005007841A1 (pt)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0200350B1 (pt) * 2001-02-13 2014-10-14 Ajinomoto Kk Bactéria transgênica produtora de l-aminoácido pertencente ao gênero escherichia, e, método para produzir l-aminoácido
RU2229513C2 (ru) * 2001-11-23 2004-05-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Способ получения l-аминокислот, штамм escherichia coli - продуцент l-аминокислоты (варианты)
JP4479283B2 (ja) * 2004-03-04 2010-06-09 味の素株式会社 Ｌ−システイン生産菌及びｌ−システインの製造法
WO2006088235A1 (en) 2005-02-18 2006-08-24 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family
WO2006123764A1 (en) * 2005-05-16 2006-11-23 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family with attenuated expression of the kefb gene
EP1976994A1 (en) * 2006-01-26 2008-10-08 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of enterobacteriaceae family with attenuated expression of the aldh gene
EP2004803A2 (en) 2006-03-23 2008-12-24 Ajinomoto Co., Inc. A method for producing an l-amino acid using bacterium of theenterobacteriaceae family with attenuated expression of a gene coding for small rna
EP2007873B1 (en) * 2006-04-18 2015-11-18 Ajinomoto Co., Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE sfmACDFH-fimZ CLUSTER OR THE fimZ GENE
EP2035569A1 (en) * 2006-06-01 2009-03-18 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family with attenuated expression of the rcsa gene
RU2337961C2 (ru) * 2006-07-04 2008-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) СПОСОБ ПОЛУЧЕНИЯ L-ТРЕОНИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia, В КОТОРОЙ ИНАКТИВИРОВАН ОПЕРОН rspAB
RU2006143864A (ru) * 2006-12-12 2008-06-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНОВ cynT, cynS, cynX, ИЛИ cynR, ИЛИ ИХ КОМБИНАЦИИ
DE102007007333A1 (de) 2007-02-14 2008-08-21 Wacker Chemie Ag Verfahren zur Reinigung von L-Cystein
EP2123756B1 (en) * 2007-02-15 2014-06-04 Ajinomoto Co., Inc. Transglutaminase having disulfide bond introduced therein
WO2009091045A1 (ja) * 2008-01-18 2009-07-23 Ajinomoto Co., Inc. セリン誘導体の製造方法及びこれに用いるタンパク質
JP5476545B2 (ja) * 2008-02-21 2014-04-23 味の素株式会社 Ｌ−システイン生産菌及びｌ−システインの製造法
DE602009000714D1 (de) * 2008-03-06 2011-03-24 Ajinomoto Kk L-Zystein-produzierendes Bakterium und Verfahren zur Herstellung von L-Zystein
JP5332237B2 (ja) * 2008-03-06 2013-11-06 味の素株式会社 Ｌ−システイン生産菌及びｌ−システインの製造法
PE20110369A1 (es) 2008-09-08 2011-06-24 Ajinomoto Kk Un microorganismo que produce l-aminoacido y un metodo para producir un l-aminoacido
RU2008148283A (ru) * 2008-12-09 2010-06-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) СПОСОБ ПОЛУЧЕНИЯ L-АРГИНИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА artI
JP5521347B2 (ja) * 2009-02-16 2014-06-11 味の素株式会社 Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造法
JP5463528B2 (ja) * 2009-02-25 2014-04-09 味の素株式会社 Ｌ−システイン生産菌及びｌ−システインの製造法
JP5359409B2 (ja) 2009-03-12 2013-12-04 味の素株式会社 Ｌ−システイン生産菌及びｌ−システインの製造法
RU2009136544A (ru) 2009-10-05 2011-04-10 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) (RU) Способ получения l-цистеина с использованием бактерии семейства enterobacteriaceae
BR112012012915B1 (pt) 2009-11-30 2020-12-01 Ajinomoto Co., Inc. método para produção de l-cisteína, l-cistina, um derivado das mesmas, ou uma mistura das mesmas
RU2460793C2 (ru) 2010-01-15 2012-09-10 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) Способ получения l-аминокислот с использованием бактерий семейства enterobacteriaceae
JP5803927B2 (ja) 2010-09-14 2015-11-04 味の素株式会社 含硫アミノ酸生産菌及び含硫アミノ酸の製造法
JP2014087259A (ja) 2011-02-22 2014-05-15 Ajinomoto Co Inc Ｌ−システイン生産菌及びｌ−システインの製造法
EP2695940B1 (en) 2011-04-01 2016-11-30 Ajinomoto Co., Inc. Method for producing l-cysteine
US9234223B2 (en) 2011-04-01 2016-01-12 Ajinomoto Co., Inc. Method for producing L-cysteine
JP2014131487A (ja) * 2011-04-18 2014-07-17 Ajinomoto Co Inc Ｌ−システインの製造法
CN105008532B (zh) 2013-05-13 2017-07-21 味之素株式会社 L‑氨基酸的制造方法
JP2016165225A (ja) 2013-07-09 2016-09-15 味の素株式会社 有用物質の製造方法
JP6459962B2 (ja) 2013-10-21 2019-01-30 味の素株式会社 Ｌ−アミノ酸の製造法
JP7066977B2 (ja) 2017-04-03 2022-05-16 味の素株式会社 Ｌ－アミノ酸の製造法
KR20190092950A (ko) 2018-01-31 2019-08-08 씨제이제일제당 (주) 연속식 크로마토그래피 공정을 이용한 천연 l-시스테인 염산염 수화물 결정의 제조 방법
KR20190092951A (ko) 2018-01-31 2019-08-08 씨제이제일제당 (주) 연속식 크로마토그래피 공정을 이용한 천연 l-시스테인 결정의 제조 방법
WO2020071538A1 (en) 2018-10-05 2020-04-09 Ajinomoto Co., Inc. Method for producing target substance by bacterial fermentation
JP7491314B2 (ja) 2019-02-22 2024-05-28 味の素株式会社 ｙｄｉＪ遺伝子を過剰発現する腸内細菌科に属する細菌を用いたＬ－アミノ酸の製造方法
JP7524909B2 (ja) 2019-04-05 2024-07-30 味の素株式会社 Ｌ－アミノ酸の製造方法
JP7655312B2 (ja) 2019-09-25 2025-04-02 味の素株式会社 細菌の発酵によるｌ－アミノ酸の製造方法
KR102277404B1 (ko) * 2021-01-27 2021-07-14 씨제이제일제당 주식회사 신규한 갈락토사이드 o-아세틸트랜스퍼라제 변이체 및 이를 이용한 l-글루탐산 생산 방법
EP4784722A2 (en) * 2023-09-29 2026-08-05 Northeastern University Methods to modulate in vivo levels of hydrogen sulfide by engineered organisms
CN121182776B (zh) * 2025-11-25 2026-02-03 山东理工大学 一种磷酸胆碱胞苷酰转移酶突变体及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU875663A1 (ru) 1978-06-30 1982-09-15 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Штаммы е.coLI ВНИИГенетика VL 334 @ N6 и ВНИИГенетика VL 334 @ N7-продуценты L-треонина и способ их получени
JPS5618596A (en) 1979-07-23 1981-02-21 Ajinomoto Co Inc Production of l-lysine through fermentation process
US5976843A (en) 1992-04-22 1999-11-02 Ajinomoto Co., Inc. Bacterial strain of Escherichia coli BKIIM B-3996 as the producer of L-threonine
ES2158867T3 (es) 1992-11-10 2001-09-16 Ajinomoto Kk Dna que codifica mutantes de aspartoquinasa iii y su uso para la produccion de l-treonina por fermentacion.
JPH07155184A (ja) 1993-12-08 1995-06-20 Ajinomoto Co Inc 発酵法によるｌ−リジンの製造法
DE19539952A1 (de) 1995-10-26 1997-04-30 Consortium Elektrochem Ind Verfahren zur Herstellung von O-Acetylserin, L-Cystein und L-Cystein-verwandten Produkten
JPH11299491A (ja) * 1997-10-08 1999-11-02 Research Institute Of Innovative Technology For The Earth 改変セリンアセチルトランスフェラーゼ、改変セリンアセチルトランスフェラーゼ遺伝子、新規な組み換え体ｄｎａ及び改変セリンアセチルトランスフェラーゼの製造法
JP4151094B2 (ja) * 1997-11-25 2008-09-17 味の素株式会社 Ｌ−システインの製造法
RU2229513C2 (ru) 2001-11-23 2004-05-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Способ получения l-аминокислот, штамм escherichia coli - продуцент l-аминокислоты (варианты)

Also Published As

Publication number Publication date
US7312058B2 (en) 2007-12-25
EP1650296B1 (en) 2012-04-18
JP4923573B2 (ja) 2012-04-25
US20050112731A1 (en) 2005-05-26
JPWO2005007841A1 (ja) 2006-11-09
EP1650296A1 (en) 2006-04-26
WO2005007841A1 (ja) 2005-01-27
EP1650296A4 (en) 2006-07-12
ATE554162T1 (de) 2012-05-15

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