ATE520771T1 - Mutierte glucose-dehydrogenase - Google Patents

Mutierte glucose-dehydrogenase

Info

Publication number
ATE520771T1
ATE520771T1 AT09004339T AT09004339T ATE520771T1 AT E520771 T1 ATE520771 T1 AT E520771T1 AT 09004339 T AT09004339 T AT 09004339T AT 09004339 T AT09004339 T AT 09004339T AT E520771 T1 ATE520771 T1 AT E520771T1
Authority
AT
Austria
Prior art keywords
amino acid
glucose dehydrogenase
acid sequence
acid residue
mutated glucose
Prior art date
Application number
AT09004339T
Other languages
English (en)
Inventor
Hideaki Yamaoka
Masashi Tsukada
Original Assignee
Arkray Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arkray Inc filed Critical Arkray Inc
Application granted granted Critical
Publication of ATE520771T1 publication Critical patent/ATE520771T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01049—Glucose-6-phosphate dehydrogenase (1.1.1.49)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Emergency Medicine (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT09004339T 2005-06-20 2006-06-12 Mutierte glucose-dehydrogenase ATE520771T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2005179231 2005-06-20

Publications (1)

Publication Number Publication Date
ATE520771T1 true ATE520771T1 (de) 2011-09-15

Family

ID=37570313

Family Applications (2)

Application Number Title Priority Date Filing Date
AT06757259T ATE455848T1 (de) 2005-06-20 2006-06-12 Mutante glucose-dehydrogenase
AT09004339T ATE520771T1 (de) 2005-06-20 2006-06-12 Mutierte glucose-dehydrogenase

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT06757259T ATE455848T1 (de) 2005-06-20 2006-06-12 Mutante glucose-dehydrogenase

Country Status (9)

Country Link
US (2) US7604969B2 (de)
EP (2) EP1860183B1 (de)
JP (1) JP5341312B2 (de)
KR (2) KR101387240B1 (de)
CN (1) CN101107353B (de)
AT (2) ATE455848T1 (de)
DE (1) DE602006011858D1 (de)
TW (2) TWI485249B (de)
WO (1) WO2006137283A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4494978B2 (ja) 2002-12-24 2010-06-30 池田食研株式会社 補酵素結合型グルコース脱水素酵素
EP1661516B1 (de) * 2003-09-02 2012-08-15 Koji Sode Glukosesensor und glukosespiegel-messgerät
EP2368987A1 (de) 2005-03-25 2011-09-28 Ikeda Food Research Co. Ltd. Coenzym-verknüpfte glucose-dehydrogenase und dafür codierendes polynucleotid
EP2241621B1 (de) * 2007-12-28 2012-08-15 Ikeda Food Research Co. Ltd. Modifiziertes glucosedehydrogenasegen
CA2759911A1 (en) * 2009-04-30 2010-11-04 Ikeda Food Research Co. Ltd. Protein electron mediator
JP5803081B2 (ja) * 2009-10-09 2015-11-04 東洋紡株式会社 Ｆａｄジヌクレオチド依存性グルコースデヒドロゲナーゼの温度依存性を改善する方法
WO2012010308A1 (en) * 2010-07-23 2012-01-26 Roche Diagnostics Gmbh Zwitterion buffer containing compositions and uses in electroanalytical devices and methods
JP5824205B2 (ja) * 2010-10-27 2015-11-25 アークレイ株式会社 変異グルコース脱水素酵素
CN102559624B (zh) * 2012-03-05 2013-10-16 浙江德清汇宁生物科技有限公司 一种吡咯喹啉醌依赖型葡萄糖脱氢酶的热稳定性突变体及其高通量筛选方法
US10487348B2 (en) * 2014-08-12 2019-11-26 Pharmazell Gmbh 3-alpha-hydroxysteroid dehydrogenase mutants and process for the preparation of ursodeoxycholic acid
WO2016087937A2 (en) * 2014-12-04 2016-06-09 Smartzyme Innovation Ltd. Compositions and methods for measuring blood glucose levels
CN106349392A (zh) * 2015-07-14 2017-01-25 达尔生技股份有限公司 一种融合多肽
EP3325615A4 (de) 2015-07-23 2019-06-05 Smartzyme Biopharma Ltd. Zusammensetzungen und verfahren zur messung des blutzuckerspiegels
JP6856344B2 (ja) * 2015-10-29 2021-04-07 アークレイ株式会社 変異グルコース脱水素酵素およびその利用
EP3249050B1 (de) 2016-05-23 2019-01-23 ARKRAY, Inc. Enzymelektrode und biosensor mit verwendung davon
JP7339723B2 (ja) * 2017-07-04 2023-09-06 アークレイ株式会社 変異型シトクロムタンパク質およびその利用
CN109425643B (zh) 2017-08-25 2022-10-25 爱科来株式会社 基于酶电化学阻抗测量法的新型生物传感技术
CN108486027A (zh) * 2018-04-04 2018-09-04 江南大学 一种fad为辅基的葡萄糖脱氢酶的生产、纯化方法
EP3578665B1 (de) 2018-05-22 2024-02-21 ARKRAY, Inc. Biosensing-verfahren
US11293921B2 (en) 2018-10-03 2022-04-05 Arkray, Inc. Direct electron transfer-type oxidoreductase-modified molecular recognition element
CN113614514B (zh) * 2020-01-07 2022-11-25 北京九强生物技术股份有限公司 6-磷酸葡萄糖脱氢酶突变体及其在制备检测试剂中的用途
CN115125221A (zh) 2021-03-29 2022-09-30 北卡罗来纳大学教堂山分校 具有改善的热稳定性的突变体葡萄糖脱氢酶及其应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10243786A (ja) 1997-03-03 1998-09-14 Koji Hayade 改変型グルコース脱水素酵素
JP2000312588A (ja) * 1999-04-30 2000-11-14 Koji Hayade グルコース脱水素酵素
JP2001197888A (ja) 2000-01-18 2001-07-24 Koji Hayade 基質特異性に優れたグルコース脱水素酵素
TWI224136B (en) * 1999-04-30 2004-11-21 Koji Sode Glucose dehydrogenase
PL205561B1 (pl) * 2000-10-27 2010-05-31 Hoffmann La Roche Zmutowane białko zależnej od pirolochinolinochinonu rozpuszczalnej dehydrogenazy glukozowej, wyizolowany polinukleotyd kodujący to białko, wektor ekspresyjny, komórka gospodarza, sposób wytwarzania wariantów s-GDH, sposób wykrywania, oznaczania lub pomiaru glukozy i urządzenie do wykrywania lub pomiaru glukozy
CA2427031C (en) 2000-10-31 2016-02-09 Koji Sode Novel glucose dehydrogenase and method for producing the dehydrogenase
JP4036667B2 (ja) 2002-03-25 2008-01-23 広司 早出 新規グルコース脱水素酵素及びそれをコードする遺伝子
JP4107389B2 (ja) 2002-04-26 2008-06-25 広司 早出 グルコース脱水素酵素βサブユニット及びそれをコードするＤＮＡ
JP2004344145A (ja) 2002-05-27 2004-12-09 Toyobo Co Ltd 基質特異性または安定性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
US7476525B2 (en) * 2002-05-27 2009-01-13 Toyo Boseki Kabushiki Kaisha Modified pyrroloquinoline quinone (PQQ) dependent glucose dehydrogenase with superior substrate specificity and stability
JP4102138B2 (ja) * 2002-08-30 2008-06-18 広司 早出 グルコース脱水素酵素の製造方法
JP2004173538A (ja) 2002-11-25 2004-06-24 Amano Enzyme Inc ピロロキノリンキノン依存性グルコース脱水素酵素
JP4332794B2 (ja) 2003-03-24 2009-09-16 東洋紡績株式会社 基質特異性または安定性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
JP4029346B2 (ja) 2003-03-24 2008-01-09 東洋紡績株式会社 基質特異性または安定性に優れたピロロキノリンキノン（ｐｑｑ）依存性グルコースデヒドロゲナーゼ改変体
US7803592B2 (en) * 2004-04-23 2010-09-28 Arkray, Inc. Mutant glucose dehydrogenase
EP1600503A1 (de) * 2004-05-14 2005-11-30 Amano Enzyme Inc. Modifizierte pyrroloquinolin quinon-abhängige Glukose Dehydrogenase

Also Published As

Publication number Publication date
ATE455848T1 (de) 2010-02-15
JP5341312B2 (ja) 2013-11-13
CN101107353A (zh) 2008-01-16
KR20080028834A (ko) 2008-04-01
JPWO2006137283A1 (ja) 2009-01-15
US20080206833A1 (en) 2008-08-28
EP2077321A2 (de) 2009-07-08
EP2077321B1 (de) 2011-08-17
EP1860183A1 (de) 2007-11-28
TW200726842A (en) 2007-07-16
WO2006137283A1 (ja) 2006-12-28
KR101387240B1 (ko) 2014-04-21
KR101399727B1 (ko) 2014-05-29
TW201307562A (zh) 2013-02-16
EP1860183A4 (de) 2008-10-15
DE602006011858D1 (de) 2010-03-11
US20100086989A1 (en) 2010-04-08
TWI485249B (zh) 2015-05-21
TWI403584B (zh) 2013-08-01
KR20130018336A (ko) 2013-02-20
US7604969B2 (en) 2009-10-20
CN101107353B (zh) 2015-07-29
EP1860183B1 (de) 2010-01-20
EP2077321A3 (de) 2009-10-07
US7776575B2 (en) 2010-08-17

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