BRPI0506474A - sistema de tecido com células tronco não diferenciadas derivadas no limbus da córnea - Google Patents

sistema de tecido com células tronco não diferenciadas derivadas no limbus da córnea

Info

Publication number
BRPI0506474A
BRPI0506474A BRPI0506474-0A BRPI0506474A BRPI0506474A BR PI0506474 A BRPI0506474 A BR PI0506474A BR PI0506474 A BRPI0506474 A BR PI0506474A BR PI0506474 A BRPI0506474 A BR PI0506474A
Authority
BR
Brazil
Prior art keywords
tissue system
tissue
stem cells
limbic
uscs
Prior art date
Application number
BRPI0506474-0A
Other languages
English (en)
Inventor
Satish Totey Mahadeorao
Sudhadra Devi Kashyap
Original Assignee
Reliance Life Sciences Pvt Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Reliance Life Sciences Pvt Ltd filed Critical Reliance Life Sciences Pvt Ltd
Publication of BRPI0506474A publication Critical patent/BRPI0506474A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/02—Coculture with; Conditioned medium produced by embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Vascular Medicine (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

"SISTEMA DE TECIDO COM CéLULAS TRONCO NãO DIFERENCIADAS DERIVADAS DO LIMBUS DA CóRNEA". A presente descrição apresenta um sistema de tecido com células tronco límbicas auto-regenerativas, em que as célula tronco límbicas são principalmente células tronco não diferenciadas (USCS). O sistema de tecido é derivado do tecido límbico da córnea isolado, e é apropriado para restaurar deteriorações da superfície ocular, particularmente aquelas que resultam das deficiências das células tronco límbicas. O sistema de tecido é gerado ao aumentar seletivamente o sistema de tecido para as USCS, por exemplo, mediante a seleção e a classificação das células que expressam marcadores de superfície específicos de células tronco, tal como o marcador 4 de antígeno embriónico específico de estágio (SSEA-4) . Depois do isolamento, as USCs são cultivadas em uma base de tecido na presença de um meio enriquecido para gerar o sistema de tecido, o qual é apropriado para o transplante, o implante, ou o enxerto.
BRPI0506474-0A 2004-01-27 2005-01-27 sistema de tecido com células tronco não diferenciadas derivadas no limbus da córnea BRPI0506474A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN74MU2004 2004-01-27
PCT/IB2005/000203 WO2005079145A2 (en) 2004-01-27 2005-01-27 Tissue system with undifferentiated stem cells derived from corneal limbus

Publications (1)

Publication Number Publication Date
BRPI0506474A true BRPI0506474A (pt) 2007-02-06

Family

ID=34856876

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0506474-0A BRPI0506474A (pt) 2004-01-27 2005-01-27 sistema de tecido com células tronco não diferenciadas derivadas no limbus da córnea

Country Status (10)

Country Link
US (1) US20050186672A1 (pt)
EP (1) EP1733026A4 (pt)
JP (1) JP2007524411A (pt)
KR (1) KR20070001108A (pt)
CN (1) CN100554411C (pt)
BR (1) BRPI0506474A (pt)
CA (1) CA2554691A1 (pt)
SG (1) SG151246A1 (pt)
WO (1) WO2005079145A2 (pt)
ZA (1) ZA200606102B (pt)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1290028B1 (en) 2000-06-05 2009-12-23 The Brigham And Women's Hospital, Inc. A GENE ENCODING A MULTIDRUG RESISTANCE HUMAN P-GLYCOPROTEIN HOMOLOGUE ON CHROMOSOME 7p15-21 AND USES THEREOF
WO2006088747A2 (en) * 2005-02-14 2006-08-24 Mercer University Serum-free reagents for the isolation, cultivation, and cryopreservation of postnatal pluripotent epiblast-like stem cells
WO2007059084A2 (en) * 2005-11-14 2007-05-24 The New England Medical Center Hospitals, Inc. Methods for preparing cord matrix stem cells (cmsc) for long term storage and for preparing a segment of umbilical cord for cryopreservation
CN101389754A (zh) 2005-12-29 2009-03-18 人类起源公司 胎盘干细胞和第二来源干细胞的联合培养
JP2009527250A (ja) * 2006-02-24 2009-07-30 リライアンス ライフ サイエンシーズ プライベイト リミテッド 結膜組織系
KR100908481B1 (ko) 2006-04-24 2009-07-21 코아스템(주) 중간엽 줄기세포 배양 배지 및 이를 이용한 중간엽줄기세포의 배양 방법
HUE059011T2 (hu) 2006-05-31 2022-09-28 Childrens Medical Center Immunmodulátorként szolgáló ABC5-pozitív mezenchimális õssejtek
WO2008024832A2 (en) * 2006-08-24 2008-02-28 Cedars-Sinai Medical Center Methods for isolating and using pituitary adenoma stem cells and pituitary adenoma cells
US20100062477A1 (en) * 2006-11-28 2010-03-11 Cedars-Sinai Medical Center Methods of isolating and propagating stem cells from benign tumors
CA2685248A1 (en) * 2007-04-26 2008-11-06 Medinnova As Transplant storage
US20100272694A1 (en) * 2008-10-17 2010-10-28 Affiliated Hospital Of Ningxia Medical University Clinic compliant method for banking human placental mesenchymal cells
US11542328B2 (en) 2008-11-14 2023-01-03 The Brigham And Women's Hospital, Inc. Therapeutic and diagnostic methods relating to cancer stem cells
WO2010104696A1 (en) * 2009-03-11 2010-09-16 Invitrx, Inc. Method and composition for the treatment of moderate to severe keratoconjunctivitis sicca
EP2309003A1 (en) * 2009-09-18 2011-04-13 Bioftalmik, S.L. Method for the diagnosis of limbal stem cell deficiency
EP2499240A1 (en) 2009-11-12 2012-09-19 Vbi Technologies, LLC Subpopulations of spore-like cells and uses thereof
KR101202836B1 (ko) * 2010-08-27 2012-11-20 서울대학교산학협력단 세포응집을 이용한 인간 혈액 유래 혈구 세포괴의 유도와 이를 이용한 혈중 성체줄기세포 및 전구세포의 증폭방법 및 상기의 방법에 의해 제조된 줄기세포
JP5759536B2 (ja) * 2011-04-20 2015-08-05 国立大学法人大阪大学 角膜上皮分化指向性ｉＰＳ細胞
RU2475218C1 (ru) * 2011-12-06 2013-02-20 Федеральное государственное учреждение "Межотраслевой научно-технический комплекс "Микрохирургия глаза" имени академика С.Н. Федорова Федерального агентства по высокотехнологичной медицинской помощи" Способ выделения и органо-типической консервации аллогенного лимбального трансплантата
WO2013184843A1 (en) * 2012-06-05 2013-12-12 The Regents Of The University Of California Novel methods to regenerate human limbal stem cells
US9308082B2 (en) 2012-08-07 2016-04-12 RegenEye, L.L.C. Ocular collar stent for treating narrowing of the irideocorneal angle
US10265161B2 (en) 2012-08-07 2019-04-23 Regeneye L. L. C. Ocular collar stent for treating narrowing of the irideocorneal angle
US9974645B2 (en) 2012-08-07 2018-05-22 RegenEye, L.L.C. Method of reducing the occurrence of macular and neuroretinal degenerations by alleviating age related retinal stresses as a contributing factor in a mammalian eye
AU2014219026B2 (en) * 2013-02-19 2018-11-29 Children's Medical Center Corporation ABCB5(+) stem cells for treating ocular disease
CN107075469A (zh) * 2014-06-27 2017-08-18 加利福尼亚大学董事会 培养的哺乳动物角膜缘干细胞、其产生方法和其用途
KR101645901B1 (ko) * 2015-12-31 2016-08-04 가톨릭대학교 산학협력단 양막 슬라이드 지지체를 이용한 윤부줄기세포 배양방법
CN105797138A (zh) * 2016-03-29 2016-07-27 深圳爱生再生医学科技有限公司 修复角膜的干细胞制剂及其制备方法和应用
CN106039289A (zh) * 2016-05-26 2016-10-26 深圳爱生再生医学科技有限公司 干细胞制剂及其制备方法
JP2020501543A (ja) 2016-12-07 2020-01-23 メイヨ・ファウンデーション・フォー・メディカル・エデュケーション・アンド・リサーチ 網膜色素上皮移植のためのフィブリン支持体を用いた方法及び材料
CA3048388A1 (en) * 2017-01-13 2018-07-19 Osaka University Method for producing a corneal epithelial cell population
KR102624998B1 (ko) * 2017-06-05 2024-01-12 메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치 줄기 세포의 배양, 증식 및 분화를 위한 방법 및 물질
EP3645068A4 (en) * 2017-06-30 2021-04-07 Vascudyne Inc REGENERATIVE FABRIC AND NATURAL TISSUE IMPLANTS
WO2019210109A1 (en) 2018-04-25 2019-10-31 Children's Medical Center Corporation Abcb5 ligands and substrates
EP3823635A4 (en) 2018-07-19 2022-08-24 Texas Tech University System CORNEAL EPITHELIAL CELLS AND PRODUCTS THEREOF FOR THE TREATMENT OF CORNEAL DISEASES
CN109321527A (zh) * 2018-10-10 2019-02-12 中国海洋大学 角膜缘干细胞稳定性的体外培养方法
CN111700912B (zh) * 2020-06-03 2022-04-19 青岛海尔生物科技有限公司 一种适用于角膜缘干细胞缺乏症的滴眼液及制备
CN111657269B (zh) * 2020-06-22 2022-02-08 镇江雷音再生医学科技有限公司 纤维蛋白胶用于smile来源人角膜透镜保存前的保护处理方法
CN113106057B (zh) * 2020-12-23 2023-04-25 重庆医科大学附属儿童医院 一种用于肝细胞移植的自体干细胞制剂及制备方法
CN112626019A (zh) * 2020-12-28 2021-04-09 武汉爱尔眼科医院有限公司 一种眼角膜及角膜缘单细胞悬液的制备方法
CN115558636A (zh) * 2021-07-01 2023-01-03 京东方再生医学科技有限公司 无动物源性细胞膜片及其制备方法和应用

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5703047A (en) * 1992-09-21 1997-12-30 Board Of Regents, The University Of Texas System Methods and treatments for corneal healing with growth factors
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US6152142A (en) * 1997-02-28 2000-11-28 Tseng; Scheffer C. G. Grafts made from amniotic membrane; methods of separating, preserving, and using such grafts in surgeries
US7410798B2 (en) * 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US20030161817A1 (en) * 2001-03-28 2003-08-28 Young Henry E. Pluripotent embryonic-like stem cells, compositions, methods and uses thereof
US20020039788A1 (en) * 2000-02-29 2002-04-04 Isseroff Roslyn R. Corneal epithelial graft composites
EP1276431B1 (en) * 2000-04-27 2006-06-14 Ray Jui-Fang Tsai Method for expansion of epithelial stem cells
US6921665B2 (en) * 2000-11-27 2005-07-26 Roslin Institute (Edinburgh) Selective antibody targeting of undifferentiated stem cells
US20040015617A1 (en) * 2001-01-25 2004-01-22 Sangha Onkar S. Flexible network interfaces and flexible data clocking
US7347876B2 (en) * 2001-04-25 2008-03-25 Ray Jui-Fang Tsai Method for expansion of epithelial stem cells
CN1218755C (zh) * 2001-07-27 2005-09-14 北京科宇联合干细胞生物技术有限公司 一种干细胞再生表层角膜及其作为角膜移植材料的用途
ITRM20010476A1 (it) * 2001-08-03 2003-02-03 Idi Irccs Lamine di epitelio corneale umano ricostruite e metodo per il loro ottenimento.
WO2003023018A2 (en) * 2001-09-12 2003-03-20 DeveloGen Aktiengesellschaft für entwicklungsbiologische Forschung A method for isolating, culturing and differentiating intestinal stem cells for therapeutic use
CA2483010A1 (en) * 2002-04-19 2003-10-30 University Of Pittsburgh Of The Commonwealth System Of Higher Education Placental derived stem cells and uses thereof
ITUD20020092A1 (it) 2002-04-30 2002-07-30 Univ Degli Studi Udine Metodo per l'identificazione e l'isolamento di cellule staminali di un tessuto umano
US7422736B2 (en) * 2002-07-26 2008-09-09 Food Industry Research And Development Institute Somatic pluripotent cells
US20050136536A1 (en) * 2003-09-15 2005-06-23 Anderson Daniel G. Embryonic epithelial cells
KR100931887B1 (ko) * 2004-02-26 2009-12-15 리라이언스 라이프 사이언시스 프라이빗. 리미티드 각막 윤부에서 유도된 다능성 배아 유사 줄기 세포, 분리 방법 및 이들의 용도
MY165114A (en) * 2006-09-01 2018-02-28 Stempeutics Res Private Limited Self-renewing master adult pluripotent stem cells

Also Published As

Publication number Publication date
JP2007524411A (ja) 2007-08-30
CN100554411C (zh) 2009-10-28
US20050186672A1 (en) 2005-08-25
ZA200606102B (en) 2007-11-28
SG151246A1 (en) 2009-04-30
EP1733026A2 (en) 2006-12-20
WO2005079145A3 (en) 2007-05-03
WO2005079145A2 (en) 2005-09-01
CA2554691A1 (en) 2005-09-01
EP1733026A4 (en) 2009-06-03
KR20070001108A (ko) 2007-01-03
WO2005079145A8 (en) 2006-04-13
CN101052299A (zh) 2007-10-10

Similar Documents

Publication Publication Date Title
BRPI0506474A (pt) sistema de tecido com células tronco não diferenciadas derivadas no limbus da córnea
Gong et al. Isolation and characterization of lung resident mesenchymal stem cells capable of differentiating into alveolar epithelial type II cells
BR0210599A (pt) Uso de monóxido de carbono, método para manutenção de uma célula animal in vitro, artigo de manufatura, bem como meio celular estéril
BRPI0407636A (pt) meio de cultura de células, linhagem de células animais, métodos para produzir um meio de cultura condicionado, células animais, uma cultura de células animais, vìrus em uma cultura de células animais, ou, de preferência, humanas diplóides dependentes de ancoragem, método para produzir uma vacina, e, população de vìrus
MX350951B (es) Composiciones y metodos para tratar enfermedades retinales.
NO20022180D0 (no) Hematopoietisk differensiering av humane embryoniske stamceller
WO2007099556A3 (en) Conjunctival tissue system
IL210618A (en) Cell cultures containing isolated and multiplied human cells other than embryonic and germ cells, pharmaceutical preparations containing them, methods of preparation, and methods for identifying modulators and differentiation factors.
BRPI0411798A (pt) sistemas e métodos para separar e concentrar células regenerativas do tecido
CU20210033A7 (es) Métodos y composiciones para terapia con células oculares
BR112022017216A2 (pt) Método para produzir células exterminadoras naturais a partir de células-tronco pluripotentes
ATE482267T1 (de) Tierzellen und verfahren für die replikation von influenza viren
ATE489456T1 (de) Menschliche hornhautendothelzellen und verfahren zur gewinnung und kultivierung von zellen zur hornhautzelltransplantation
BR0212780A (pt) Métodos para produzir polipeptìdeos em células eucarióticas, para cultivar células eucarióticas e para colher polipeptìdeos produzidos por células eucarióticas
NO20051030L (no) Funksjonelle kardiomyocytter fra humane embryonale stamceller
UY38321A (es) Proceso de obtención de un sustituto dérmico a partir de membrana amniótica descelularizada y su uso como agente de regeneración
BR112022014246A2 (pt) Meio de criopreservação para criopreservar células de mamífero, kit, e, métodos de criopreservação de células de mamífero e recuperação de células viáveis
Echevarria et al. Tissue-regenerating, vision-restoring corneal epithelial stem cells
ATE329632T1 (de) Verfahren zur entwicklung von epithelialen stammzellen
Li et al. In vivo dedifferentiation of human epidermal cells
NZ551828A (en) A method of cell isolating a homogenous population of mammary stem cells using specific cell surface marker proteins
WO2004113515A3 (en) Tissue modeling in embryonic stem (es) cell system
Stoner et al. Putrescine stimulates growth of human bronchial epithelial cells in primary culture
Wang et al. Characterization of ex vivo expanded oral mucosal epithelium cells on acellular porcine corneal stroma for ocular surface reconstruction
BRPI0407002A (pt) Células cultivadas que apresentam antìgeno cd14+

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 6A ANUIDADE(S).

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2094 DE 22/02/2011.

B15K Others concerning applications: alteration of classification

Ipc: C12N 5/079 (2010.01), C12N 5/0797 (2010.01)