ECSP077295A - Sistemas y metodos para la produccion de proteinas - Google Patents
Sistemas y metodos para la produccion de proteinasInfo
- Publication number
- ECSP077295A ECSP077295A EC2007007295A ECSP077295A ECSP077295A EC SP077295 A ECSP077295 A EC SP077295A EC 2007007295 A EC2007007295 A EC 2007007295A EC SP077295 A ECSP077295 A EC SP077295A EC SP077295 A ECSP077295 A EC SP077295A
- Authority
- EC
- Ecuador
- Prior art keywords
- cells
- protein
- interest
- genetically engineered
- polypeptide
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
- C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/51—Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
La invención se refiere a sistemas y métodos para producir proteínas de interés. La invención emplea células animales o vegetales genéticamente manipuladas que tienen capacidades modificadas de doblamiento o procesamiento de proteínas. En un aspecto, la invención incorpora células genéticamente modificadas que comprenden uno o más casetes de expresión recombinantes los cuales codifican para (1) una proteína de interés y (2) un polipéptido que es funcional en la vía de respuesta de proteínas no dobladas (UPR) de las células. La co-expresión del polipéptido incrementa significativamente el rendimiento de la proteína de interés en las células genéticamente manipuladas. En un ejemplo, las células genéticamente manipuladas son células animales, y el polipéptido co-expresado es un componente o modulador de una vía de UPR mediada por XBP1 o ATF6.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US60643904P | 2004-09-02 | 2004-09-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ECSP077295A true ECSP077295A (es) | 2007-04-26 |
Family
ID=36036848
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EC2007007295A ECSP077295A (es) | 2004-09-02 | 2007-03-02 | Sistemas y metodos para la produccion de proteinas |
Country Status (16)
| Country | Link |
|---|---|
| US (3) | US7514237B2 (es) |
| EP (2) | EP2316955B1 (es) |
| JP (2) | JP2008511335A (es) |
| KR (1) | KR20070053732A (es) |
| CN (1) | CN101048509A (es) |
| AU (1) | AU2005282746A1 (es) |
| BR (1) | BRPI0514870A (es) |
| CA (1) | CA2577073C (es) |
| CR (1) | CR8927A (es) |
| EC (1) | ECSP077295A (es) |
| ES (2) | ES2649862T3 (es) |
| IL (1) | IL181278A0 (es) |
| MX (1) | MX2007002379A (es) |
| NO (1) | NO20071221L (es) |
| RU (1) | RU2007106442A (es) |
| WO (1) | WO2006028889A2 (es) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5548932B2 (ja) * | 2006-09-25 | 2014-07-16 | 国立大学法人大阪大学 | Ａｔｆ４および／またはｇａｄｄ３４を利用した蛋白質高生産方法 |
| EP2297331B1 (en) * | 2008-05-28 | 2015-03-25 | Bayer HealthCare, LLC | Methods and compositions for production of recombinant protein in hbx-expressing mammalian cells |
| CA2726804C (en) | 2008-06-02 | 2021-12-28 | Dana-Farber Cancer Institute, Inc. | Xbp1, cd138, and cs1 peptides |
| GB0902180D0 (en) | 2009-02-10 | 2009-03-25 | Ucb Pharma Sa | Method for producing protein |
| US9315565B2 (en) | 2009-02-10 | 2016-04-19 | Ucb Pharma, S.A. | Method for producing protein |
| WO2010151827A1 (en) * | 2009-06-25 | 2010-12-29 | Bayer Healthcare Llc | X-box binding proteins (xbp-1) variants and methods of using the same |
| TW201823460A (zh) | 2012-05-29 | 2018-07-01 | 美商再生元醫藥公司 | 生產細胞株增強子 |
| CL2015003242A1 (es) | 2015-11-04 | 2016-10-14 | Univ Chile | Virus aav/upr-plus, proteína de fusión upr-plus, método de tratamiento genético y su uso en el tratamiento enfermedades neurodegenerativas, tales como las enfermedades de parkinson y huntington, entre otras. |
| WO2019010551A1 (pt) * | 2017-07-10 | 2019-01-17 | Instituto De Biologia Molecular Do Paraná - Ibmp | Plataforma genética para superexpressão heteróloga associada à seleção de células altamente produtoras de proteínas |
| CN107858378B (zh) * | 2017-11-20 | 2020-11-10 | 中国科学院生物物理研究所 | 衰老细胞模型及其制备方法 |
| CN110093361B (zh) * | 2019-04-17 | 2023-05-09 | 拜澳泰克(沈阳)生物医学集团有限公司 | 一种增强基因表达的增强子多肽及其应用 |
| KR20230078719A (ko) | 2020-09-28 | 2023-06-02 | 베스타론 코포레이션 | 해충 방제용 뮤-디구에톡신-Dc1a 변이형 폴리펩타이드 |
| CN116670282A (zh) * | 2020-12-22 | 2023-08-29 | 豪夫迈·罗氏有限公司 | 靶向xbp1的寡核苷酸 |
| US20240374761A1 (en) | 2021-05-05 | 2024-11-14 | Radius Pharmaceuticals, Inc. | Animal model having homologous recombination of mouse pth1 receptor |
| WO2023122805A1 (en) | 2021-12-20 | 2023-06-29 | Vestaron Corporation | Sorbitol driven selection pressure method |
| EP4413121A4 (en) | 2022-06-09 | 2025-06-04 | CHO Plus Inc. | Cell hybrids as host cells for high efficiency production of gene therapy vectors and viral vaccines |
| WO2024026406A2 (en) | 2022-07-29 | 2024-02-01 | Vestaron Corporation | Next Generation ACTX Peptides |
| WO2025024776A2 (en) * | 2023-07-27 | 2025-01-30 | Duke University | Compositions comprising xbp1 fragments and methods of use in gene therapy |
| WO2026015884A1 (en) * | 2024-07-11 | 2026-01-15 | Helix Nanotechnologies Inc | Compositions comprising ire-1 binding sequences and uses thereof |
| CN120888609B (zh) * | 2025-10-10 | 2025-12-23 | 汇智和源生物技术(苏州)有限公司 | 一种瞬时高效表达人源slc转运体oatp1b3的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6063630A (en) | 1991-11-05 | 2000-05-16 | Transkaryotic Therapies, Inc. | Targeted introduction of DNA into primary or secondary cells and their use for gene therapy |
| AU745960C (en) | 1997-11-18 | 2003-09-18 | Pioneer Hi-Bred International, Inc. | A novel method for the integration of foreign DNA into eukaryoticgenomes |
| AU4903099A (en) | 1998-07-01 | 2000-01-24 | Austrian Nordic Biotherapeutics Ag | Targeted integration into chromosomes using retroviral vectors |
| JP2001054391A (ja) * | 1998-11-13 | 2001-02-27 | Hsp Kenkyusho:Kk | 小胞体ストレス転写因子 |
| WO2001072783A2 (en) | 2000-03-24 | 2001-10-04 | Genencor International, Inc. | Production of secreted proteins by recombinant eukaryotic cells |
| WO2003089622A2 (en) | 2002-04-22 | 2003-10-30 | University Of Michigan | Novel genes, compositions, and methods for modulating the unfolded protein response |
| JP4446057B2 (ja) * | 2002-08-28 | 2010-04-07 | 独立行政法人理化学研究所 | 生体刺激存在下でのｍＲＮＡのフレームシフトを利用した蛋白質の発現方法 |
| JP2007500016A (ja) * | 2003-06-11 | 2007-01-11 | バイオジェン・アイデック・エムエイ・インコーポレイテッド | 培養においてタンパク質生成を増大させるための方法 |
-
2005
- 2005-09-01 CA CA2577073A patent/CA2577073C/en not_active Expired - Fee Related
- 2005-09-01 US US11/217,137 patent/US7514237B2/en not_active Expired - Lifetime
- 2005-09-01 JP JP2007530343A patent/JP2008511335A/ja active Pending
- 2005-09-01 ES ES10182800.2T patent/ES2649862T3/es not_active Expired - Lifetime
- 2005-09-01 EP EP10182800.2A patent/EP2316955B1/en not_active Expired - Lifetime
- 2005-09-01 AU AU2005282746A patent/AU2005282746A1/en not_active Abandoned
- 2005-09-01 MX MX2007002379A patent/MX2007002379A/es active IP Right Grant
- 2005-09-01 KR KR1020077005169A patent/KR20070053732A/ko not_active Ceased
- 2005-09-01 ES ES05807248.9T patent/ES2653843T3/es not_active Expired - Lifetime
- 2005-09-01 BR BRPI0514870-7A patent/BRPI0514870A/pt not_active IP Right Cessation
- 2005-09-01 WO PCT/US2005/031081 patent/WO2006028889A2/en not_active Ceased
- 2005-09-01 RU RU2007106442/13A patent/RU2007106442A/ru not_active Application Discontinuation
- 2005-09-01 EP EP05807248.9A patent/EP1784490B1/en not_active Expired - Lifetime
- 2005-09-01 CN CNA200580037014XA patent/CN101048509A/zh active Pending
-
2007
- 2007-02-12 IL IL181278A patent/IL181278A0/en unknown
- 2007-02-15 CR CR8927A patent/CR8927A/es not_active Application Discontinuation
- 2007-03-02 EC EC2007007295A patent/ECSP077295A/es unknown
- 2007-03-06 NO NO20071221A patent/NO20071221L/no not_active Application Discontinuation
-
2009
- 2009-04-06 US US12/419,018 patent/US8173394B2/en not_active Expired - Lifetime
-
2012
- 2012-03-28 JP JP2012072852A patent/JP5508460B2/ja not_active Expired - Fee Related
- 2012-04-13 US US13/446,649 patent/US20120322104A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US8173394B2 (en) | 2012-05-08 |
| US7514237B2 (en) | 2009-04-07 |
| BRPI0514870A (pt) | 2008-06-24 |
| CA2577073C (en) | 2016-10-11 |
| AU2005282746A1 (en) | 2006-03-16 |
| WO2006028889A2 (en) | 2006-03-16 |
| US20060053502A1 (en) | 2006-03-09 |
| ES2649862T3 (es) | 2018-01-16 |
| RU2007106442A (ru) | 2008-10-10 |
| US20120322104A1 (en) | 2012-12-20 |
| EP2316955A1 (en) | 2011-05-04 |
| CN101048509A (zh) | 2007-10-03 |
| US20090221035A1 (en) | 2009-09-03 |
| JP5508460B2 (ja) | 2014-05-28 |
| EP1784490B1 (en) | 2017-11-01 |
| CA2577073A1 (en) | 2006-03-16 |
| NO20071221L (no) | 2007-03-30 |
| MX2007002379A (es) | 2007-04-23 |
| KR20070053732A (ko) | 2007-05-25 |
| JP2008511335A (ja) | 2008-04-17 |
| ES2653843T3 (es) | 2018-02-09 |
| CR8927A (es) | 2007-08-28 |
| IL181278A0 (en) | 2007-07-04 |
| EP2316955B1 (en) | 2017-10-04 |
| JP2012161318A (ja) | 2012-08-30 |
| EP1784490A2 (en) | 2007-05-16 |
| WO2006028889A3 (en) | 2006-10-05 |
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