BRPI0608288A2 - método para preparar polinucleotìdeos para análise - Google Patents

método para preparar polinucleotìdeos para análise

Info

Publication number
BRPI0608288A2
BRPI0608288A2 BRPI0608288-2A BRPI0608288A BRPI0608288A2 BR PI0608288 A2 BRPI0608288 A2 BR PI0608288A2 BR PI0608288 A BRPI0608288 A BR PI0608288A BR PI0608288 A2 BRPI0608288 A2 BR PI0608288A2
Authority
BR
Brazil
Prior art keywords
polynucleotide
target
analysis
forming
hybridized
Prior art date
Application number
BRPI0608288-2A
Other languages
English (en)
Inventor
Preben Lexow
Original Assignee
Lingvitae As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lingvitae As filed Critical Lingvitae As
Publication of BRPI0608288A2 publication Critical patent/BRPI0608288A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/151—Modifications characterised by repeat or repeated sequences, e.g. VNTR, microsatellite, concatemer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

MéTODO PARA PREPARAR POLINUCLEOTìDEOS PARA ANáLISE. A presente invenção refere-se a um método para analisar um polinucleotídeo alvo tendo unidades distintas de seqúência de ácidos nucléicos compreendendo: (i) formar um primeiro polinucleotkleo o qual é um concatêmero tendo múltiplas seqúências de polinucleotídeos alvo de repetição; (ii) formar sobre o primeiro polinucleotídeo um segundo polinucleotídeo hibridizado a uma porção de um ou mais dos polinucleotì'deos alvo, de tal modo que a porção hibridizada, ou a porção não hibridizada, corresponde a uma unidade de seqúência sobre o alvo, e determinar a unidade de seqúéncia sobre o alvo.
BRPI0608288-2A 2005-03-08 2006-03-08 método para preparar polinucleotìdeos para análise BRPI0608288A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0504774.1A GB0504774D0 (en) 2005-03-08 2005-03-08 Method
PCT/GB2006/000825 WO2006095169A1 (en) 2005-03-08 2006-03-08 Method for preparing polynucleotides for analysis

Publications (1)

Publication Number Publication Date
BRPI0608288A2 true BRPI0608288A2 (pt) 2009-12-22

Family

ID=34452026

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0608288-2A BRPI0608288A2 (pt) 2005-03-08 2006-03-08 método para preparar polinucleotìdeos para análise

Country Status (13)

Country Link
US (1) US20090053699A1 (pt)
EP (1) EP1861510A1 (pt)
JP (1) JP2008532508A (pt)
KR (1) KR20080005196A (pt)
CN (1) CN101142325A (pt)
AU (1) AU2006221770A1 (pt)
BR (1) BRPI0608288A2 (pt)
CA (1) CA2600240A1 (pt)
EA (1) EA012525B1 (pt)
GB (1) GB0504774D0 (pt)
NO (1) NO20074962L (pt)
WO (1) WO2006095169A1 (pt)
ZA (1) ZA200707704B (pt)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP4592400A3 (en) 2012-10-17 2025-10-29 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
CN116334193A (zh) 2013-03-15 2023-06-27 赛拉诺斯知识产权有限责任公司 核酸扩增
US20140302504A1 (en) 2013-03-15 2014-10-09 Theranos, Inc. Nucleic Acid Amplification
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
US10081825B2 (en) * 2013-03-15 2018-09-25 Aegea Biotechnologies, Inc. Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
SG11201507273SA (en) 2013-03-15 2015-10-29 Theranos Inc Nucleic acid amplification
CA2916662C (en) 2013-06-25 2022-03-08 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
SG11201601611WA (en) 2013-09-06 2016-04-28 Theranos Inc Systems and methods for detecting infectious diseases
MX370616B (es) * 2013-11-22 2019-12-17 Theranos Ip Co Llc Amplificacion de acidos nucleicos.
WO2016077313A1 (en) 2014-11-11 2016-05-19 Bgi Shenzhen Multi-pass sequencing
EP3901282B1 (en) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CA2995422A1 (en) 2015-08-12 2017-02-16 The Chinese University Of Hong Kong Single-molecule sequencing of plasma dna
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
WO2020123316A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
EP4153776B1 (en) 2020-05-22 2025-03-05 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
ES2994976T3 (en) 2020-06-25 2025-02-05 10X Genomics Inc Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4729631A2 (en) 2020-12-21 2026-04-22 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4482979B1 (en) 2022-11-09 2025-10-01 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU649066B2 (en) * 1990-07-25 1994-05-12 Syngene, Inc. Circular extension for generating multiple nucleic acid complements
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
ATE238434T1 (de) * 1995-12-05 2003-05-15 Jorn Erland Koch Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
GB0308851D0 (en) * 2003-04-16 2003-05-21 Lingvitae As Method

Also Published As

Publication number Publication date
GB0504774D0 (en) 2005-04-13
WO2006095169A1 (en) 2006-09-14
NO20074962L (no) 2007-11-23
KR20080005196A (ko) 2008-01-10
US20090053699A1 (en) 2009-02-26
JP2008532508A (ja) 2008-08-21
CA2600240A1 (en) 2006-09-14
AU2006221770A1 (en) 2006-09-14
EA200701662A1 (ru) 2008-04-28
EP1861510A1 (en) 2007-12-05
ZA200707704B (en) 2008-12-31
EA012525B1 (ru) 2009-10-30
CN101142325A (zh) 2008-03-12

Similar Documents

Publication Publication Date Title
BRPI0608288A2 (pt) método para preparar polinucleotìdeos para análise
WO2005094363A3 (en) System, method and software arrangement for bi-allele haplotype phasing
BR0214683A (pt) Molécula de nucleotìdeo ou de nucleosìdeo, métodos de rotular uma molécula de ácido nucléico e para determinar a sequência de um polinucleotìdeo alvo de filamento único, e, kit
WO2008093098A3 (en) Methods for indexing samples and sequencing multiple nucleotide templates
ES2963242T3 (es) Conjunto de sondas para analizar una muestra de ADN y método para utilizar el mismo
BR9909200A (pt) Aerogéis de silicato de alumìnio-magnésio ou de magnésio fluorado submetidos a troca iÈnica e suportes de catalisador produzidos a partir dos mesmos
WO2004013346A8 (en) Methods and compositions for genotyping
CY1117574T1 (el) Αναλυση γονιδιωματικων κλασματων με χρηση των αριθμων πολυμορφισμων
WO2007136834A3 (en) Combined extension and ligation for nucleic acid assembly
TW200712204A (en) Method of processing and/or genome mapping of ditag sequences
SG170028A1 (en) High throughput genome sequencing on dna arrays
BRPI0407207A (pt) Método para tratar, prevenir e diagnosticar uma infecção por heliocobacter
WO2007020457A3 (en) Labelled modified guanine- containing nucleosides and nucleotides comprising a fluorophore attached to the base through a linking group comprising a polyethylene glycol spacing group and methods for their use
BRPI0511479A (pt) molécula de ligação tendo atividade neutralizadora do vìrus da raiva, variante funcional de uma molécula de ligação, imunoconjugado, molécula de ácido nucléico, vetor, hospedeiro, método para produzir uma molécula de ligação ou uma variante funcional, composição farmacêutica, uso de uma molécula de ligação, uma variante funcional, um imunoconjugado como, ou uma composição, kit, método para identificar uma molécula de ligação ou uma molécula de ácido nucléico, e, coleção de moléculas de ligação humanas na superfìcie de pacotes genéticos replicáveis
EP1417000A4 (en) SYSTEM AND METHOD FOR DETERMINING NERVOUS PROXIMITY, DIRECTION AND PATHOLOGY DURING OPERATIONS
BR0301719A (pt) Processo para diagnosticar enfarte do miocárdio e/ou estratificação de risco da sìndrome aguda das coronárias, conjunto de reagentes para realizar esse diagnóstico e utilização do mesmo
BR0009529A (pt) Sequenciamento de polinucleotìdeos usando uma helicase
BR112014014307A2 (pt) métodos e kits para detecção in situ à temperatura ambiente de um ácido nucleico alvo em uma amostra biológica
BRPI0816047A2 (pt) conjunto de oligonucleotídeos para detecção de um ácido nucleico alvo, métodos para detecção da presença ou ausência de um ácido nucleico alvo e de pelo menos dois ácidos nucleicos alvo, kit, mistura de reação e amplicon identificado por fluorescência
BRPI0517131A2 (pt) mÉtodos para simplificar Ácidos nuclÉicos microbiais por modificaÇço quÍmica de citosinas
BR0315146A (pt) Composição anti-incrustante, e, método para prevenir incrustração em um processo de ácido (met) acrìlico
BR0311768A (pt) Identificação de oligonucleotìdeos para captura, detecção e quantificação de ácido nucléico do virus da hepatite a
BR0315178A (pt) Eritropoietina: remodelagem e glicoconjugação de eritropoietina
BR0317576B1 (pt) composição de revestimento de cura à temperatura ambiente, processo para a sua preparação, bem como um polìmero acrìlico e processo para a sua preparação.
BR0315836A (pt) Derivados de [6,7-dihidro-5h-imidazol[1,2-alfa]imadazol-3-sulfonila mino]-propionamida, composição farmacêutica, uso do mesmo, bem como processo para sua preparação

Legal Events

Date Code Title Description
B08F Application fees: application dismissed [chapter 8.6 patent gazette]

Free format text: REFERENTE AS 5A E 6A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2161 DE 05/06/2012.