BRPI0610092A2 - mÉtodos para a detecÇço de um analito e de um Ácido nucleico em uma amostra, e para sintetizaÇço de uma molÉcula de Ácido nucleico ou de anÁlago de Ácido nucleico, kit reagente, usos do mesmo, de um composto, e de um mÉtodo, molÉcula de Ácido nucleico ou de anÁlogo de Ácido nucleico, produto de metalizaÇço, produto associado, e, composto - Google Patents

mÉtodos para a detecÇço de um analito e de um Ácido nucleico em uma amostra, e para sintetizaÇço de uma molÉcula de Ácido nucleico ou de anÁlago de Ácido nucleico, kit reagente, usos do mesmo, de um composto, e de um mÉtodo, molÉcula de Ácido nucleico ou de anÁlogo de Ácido nucleico, produto de metalizaÇço, produto associado, e, composto

Info

Publication number
BRPI0610092A2
BRPI0610092A2 BRPI0610092-9A BRPI0610092A BRPI0610092A2 BR PI0610092 A2 BRPI0610092 A2 BR PI0610092A2 BR PI0610092 A BRPI0610092 A BR PI0610092A BR PI0610092 A2 BRPI0610092 A2 BR PI0610092A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
compound
product
methods
synthesizing
Prior art date
Application number
BRPI0610092-9A
Other languages
English (en)
Inventor
Thomas Carell
Anja Schwoegler
Glenn Ashley Burley
Johannes Gierlich
Mohammad Reza Mofid
Original Assignee
Basf Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag filed Critical Basf Ag
Publication of BRPI0610092A2 publication Critical patent/BRPI0610092A2/pt
Publication of BRPI0610092B1 publication Critical patent/BRPI0610092B1/pt
Publication of BRPI0610092B8 publication Critical patent/BRPI0610092B8/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

MÉTODOS PARA A DETECÇçO DE UM ANALITO E DE UM ÁCIDO NUCLEICO EM UMA AMOSTRA, E PARA SINTETIZAÇçO DE UMA MOLÉCULA DE ÁCIDO NUCLEICO OU DE ANÁLOGO DE ÁCIDO NUCLEICO, KIT REAGENTE, USOS DO MESMO, DE UM COMPOSTO, E DE UM MÉTODO, MOLÉCULA DE ÁCIDO NUCLEICO OU DE ANÁLOGO DE ÁCIDO NUCLEICO, PRODUTO DE METALIZAÇçO, PRODUTO ASSOCIADO, E, COMPOSTO. A invenção refere-se a métodos e reagentes para a detecção de analitos, e.g., ácidos nucleicos. Os novos métodos e reagentes permitem uma detecção simples e sensível mesmo em amostras biológicas complexas.
BRPI0610092A 2005-05-02 2006-04-28 métodos para a detecção de um analito e de um ácido nucleico em uma amostra, e de sintetização de uma molécula de ácido nucleico ou de análogo de ácido nucleico, kit reagente, uso do mesmo, molécula de ácido nucleico ou de análogo de ácido nucleico e produto associado BRPI0610092B8 (pt)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US67678505P 2005-05-02 2005-05-02
EP05009618.9 2005-05-02
EP05009618 2005-05-02
US60/676,785 2005-05-02
US75010005P 2005-12-14 2005-12-14
US60/750,100 2005-12-14
EP05027370 2005-12-14
EP05027370.5 2005-12-14
PCT/EP2006/004017 WO2006117161A2 (en) 2005-05-02 2006-04-28 New labelling strategies for the sensitive detection of analytes

Publications (3)

Publication Number Publication Date
BRPI0610092A2 true BRPI0610092A2 (pt) 2008-12-09
BRPI0610092B1 BRPI0610092B1 (pt) 2018-03-27
BRPI0610092B8 BRPI0610092B8 (pt) 2021-07-27

Family

ID=42676666

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0610092A BRPI0610092B8 (pt) 2005-05-02 2006-04-28 métodos para a detecção de um analito e de um ácido nucleico em uma amostra, e de sintetização de uma molécula de ácido nucleico ou de análogo de ácido nucleico, kit reagente, uso do mesmo, molécula de ácido nucleico ou de análogo de ácido nucleico e produto associado

Country Status (16)

Country Link
US (1) US8129315B2 (pt)
EP (4) EP2266996A3 (pt)
JP (1) JP4944098B2 (pt)
AR (1) AR053251A1 (pt)
AT (1) ATE479699T1 (pt)
AU (1) AU2006243370B2 (pt)
BR (1) BRPI0610092B8 (pt)
CA (1) CA2607530C (pt)
DE (1) DE602006016589D1 (pt)
DK (1) DK1877415T3 (pt)
MX (1) MX2007013296A (pt)
PL (1) PL1877415T3 (pt)
PT (1) PT1877415E (pt)
SI (1) SI1877415T1 (pt)
TW (1) TWI406952B (pt)
WO (1) WO2006117161A2 (pt)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
KR101335218B1 (ko) * 2005-05-02 2013-12-12 바스프 에스이 분석물의 감응성 검출을 위한 신규한 표지화 전략
TWI406952B (zh) 2005-05-02 2013-09-01 Basf Ag 分析物之敏感偵測的新標記策略
US7910335B2 (en) 2005-10-27 2011-03-22 President And Fellows Of Harvard College Methods and compositions for labeling nucleic acids
US8114636B2 (en) * 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
EP1987068B1 (en) * 2006-02-10 2018-08-08 Life Technologies Corporation Oligosaccharide modification and labeling of proteins
TW200808975A (en) * 2006-04-28 2008-02-16 Basf Ag Molecular beacons for DNA-photography
JP5646172B2 (ja) * 2006-10-31 2014-12-24 ベースクリック ゲーエムベーハー リポーター分子の製造のためのクリックケミストリー
TW200827703A (en) * 2006-11-09 2008-07-01 Basf Ag Photographic determination of analytes
GB0718255D0 (en) * 2007-09-19 2007-10-31 Univ Edinburgh Nucleobase characterisation
EP2053131A1 (en) * 2007-10-19 2009-04-29 Ludwig-Maximilians-Universität München Method for determining methylation at deoxycytosine residues
EP2222341B1 (en) 2007-11-21 2015-02-25 University Of Georgia Research Foundation, Inc. Alkynes and methods of reacting alkynes with 1,3-dipole-functional compounds
CN104263816B (zh) * 2008-05-16 2018-10-19 生命技术公司 用于测量细胞增殖的双标记法
US8618297B2 (en) 2008-07-25 2013-12-31 Basf Se Azide substituted naphthylene or rylene imide derivatives and their use as reagents in click-reactions
WO2010010198A2 (en) 2008-07-25 2010-01-28 Basf Se New azide substituted naphthylene or rylene imide derivatives and their use as reagents in click-reactions
EP3587434A1 (en) * 2008-09-23 2020-01-01 Alnylam Pharmaceuticals Inc. Chemical modifications of monomers and oligonucleotides with click components for conjugation with ligands
EP2239053A1 (en) 2009-04-09 2010-10-13 baseclick GmbH Click chemistry on heterogeneous catalysts
EP2435461B1 (en) 2009-05-29 2017-08-09 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US11435358B2 (en) 2011-06-23 2022-09-06 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
US9480752B2 (en) 2012-07-10 2016-11-01 Baseclick Gmbh Anandamide-modified nucleic acid molecules
CN111394444A (zh) 2013-09-13 2020-07-10 生命技术公司 使用凝聚核酸颗粒的装置制备
WO2017040510A1 (en) * 2015-08-31 2017-03-09 Life Technologies Corporation Colorimetric labeling and detection methods and compositions
EP3861009A4 (en) * 2018-10-05 2023-01-11 Board of Regents, The University of Texas System DETECTION AND RELEASE OF N-TERMINAL SOLID PHASE PEPTIDE
US20240052391A1 (en) 2020-10-29 2024-02-15 Dna Script Enzymatic Synthesis of Polynucleotide Probes
CN120530202A (zh) 2023-01-26 2025-08-22 Dna斯克瑞普特公司 多核苷酸探针的酶促合成

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2209754A (en) * 1987-09-17 1989-05-24 Thomas Lee Mattson Polyaldehydic polynucleotides in use as probes,their preparation and use
JP2681061B2 (ja) * 1988-06-08 1997-11-19 ロンドン・ダイアグノスティック・インコーポレーテッド 検出可能なシグナルのセンシタイザー誘導発生を利用したアッセイ
US5723289A (en) 1990-06-11 1998-03-03 Nexstar Pharmaceuticals, Inc. Parallel selex
US5843650A (en) 1995-05-01 1998-12-01 Segev; David Nucleic acid detection and amplification by chemical linkage of oligonucleotides
EP2256133B1 (en) 1997-01-08 2016-12-14 Sigma-Aldrich Co. LLC Bioconjugation of macromolecules
US7427678B2 (en) 1998-01-08 2008-09-23 Sigma-Aldrich Co. Method for immobilizing oligonucleotides employing the cycloaddition bioconjugation method
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US7205105B2 (en) * 1999-12-08 2007-04-17 Epoch Biosciences, Inc. Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis
DE10013600A1 (de) 2000-03-18 2002-01-10 Aventis Res & Tech Gmbh & Co Reaktive Monomere für die Oligonucleotid- und Polynucleotidsynthese, modifizierte Oligonucleotide und Polynucleotiden und ein Verfahren zu deren Herstellung
US7473699B2 (en) * 2002-02-28 2009-01-06 Astrazeneca Ab 3-cyclyl-5-(nitrogen-containing 5-membered ring)methyl-oxazolidinone derivatives and their use as antibacterial agents
AU2003225799A1 (en) 2002-03-03 2003-09-29 Activx Biosciences, Inc. Tethered activity-based probes and uses thereof
ES2566761T3 (es) 2002-05-30 2016-04-15 The Scripps Research Institute Ligación de azidas y acetilenos catalizada por cobre
AU2003297859A1 (en) 2002-12-13 2004-07-09 The Trustees Of Columbia University In The City Of New York Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
KR100540509B1 (ko) * 2004-01-07 2006-01-11 학교법인 포항공과대학교 수화젤레이터로 사용되는 2'-데옥시우리딘 유도체
CA2562285A1 (en) * 2004-04-08 2006-03-16 Nimblegen Systems, Inc. Comparative genomic resequencing
KR20080000620A (ko) * 2005-04-27 2008-01-02 지멘스 메디컬 솔루션즈 유에스에이, 인크. 분자 이미징 프로브를 합성하기 위한 클릭 화학 방법
TWI406952B (zh) 2005-05-02 2013-09-01 Basf Ag 分析物之敏感偵測的新標記策略
US7910335B2 (en) 2005-10-27 2011-03-22 President And Fellows Of Harvard College Methods and compositions for labeling nucleic acids
JP5646172B2 (ja) 2006-10-31 2014-12-24 ベースクリック ゲーエムベーハー リポーター分子の製造のためのクリックケミストリー

Also Published As

Publication number Publication date
SI1877415T1 (sl) 2011-01-31
JP4944098B2 (ja) 2012-05-30
EP1877415B1 (en) 2010-09-01
EP2266996A3 (en) 2011-06-15
ATE479699T1 (de) 2010-09-15
AU2006243370A1 (en) 2006-11-09
AR053251A1 (es) 2007-04-25
WO2006117161A3 (en) 2007-01-11
CA2607530C (en) 2014-07-08
EP2266996A2 (en) 2010-12-29
WO2006117161A2 (en) 2006-11-09
DK1877415T3 (da) 2010-12-20
PL1877415T3 (pl) 2011-02-28
EP2270228A1 (en) 2011-01-05
TW200720441A (en) 2007-06-01
US8129315B2 (en) 2012-03-06
EP2256126A1 (en) 2010-12-01
EP2256126B1 (en) 2015-07-01
EP2270228B1 (en) 2013-08-21
JP2008539703A (ja) 2008-11-20
CA2607530A1 (en) 2006-11-09
US20090215635A1 (en) 2009-08-27
MX2007013296A (es) 2007-12-13
DE602006016589D1 (de) 2010-10-14
TWI406952B (zh) 2013-09-01
EP1877415A2 (en) 2008-01-16
BRPI0610092B1 (pt) 2018-03-27
BRPI0610092B8 (pt) 2021-07-27
AU2006243370B2 (en) 2012-06-28
PT1877415E (pt) 2010-12-09

Similar Documents

Publication Publication Date Title
BRPI0610092A2 (pt) mÉtodos para a detecÇço de um analito e de um Ácido nucleico em uma amostra, e para sintetizaÇço de uma molÉcula de Ácido nucleico ou de anÁlago de Ácido nucleico, kit reagente, usos do mesmo, de um composto, e de um mÉtodo, molÉcula de Ácido nucleico ou de anÁlogo de Ácido nucleico, produto de metalizaÇço, produto associado, e, composto
EP4339299A3 (en) Methods for determining a location of a target nucleic acid in a biological sample
WO2009076485A3 (en) Sequencing of nucleic acids
WO2010141075A8 (en) Specific analysis of ketone and aldehyde analytes using reagent compounds, labeling strategies, and mass spectrometry workflow
WO2011017094A3 (en) Assay apparatuses, consumables and methods
BR112012021202A2 (pt) aparelho e metodos para preparacao, reacao e deteccao integradas de amostras
WO2006116362A3 (en) Structured substrates for optical surface profiling
CL2011002228A1 (es) Aparato para el analisis integrado de acidos nucleicos en tiempo real, y metodo de detecicion de dichas moleculas.
WO2011057197A3 (en) Integrated droplet actuator for gel electrophoresis and molecular analysis
DE60023539D1 (de) Immunologischer nachweis von rna:dna hybriden auf mikroarrays
EA201071134A1 (ru) Анализ днк посредством капиллярного электрофореза
ATE431854T1 (de) Auf bio-barcodes beruhender nachweis von zielanalyten
WO2007133704A3 (en) A non-nucleic acid based biobarcode assay for detection of biological materials
WO2008080629A3 (de) Verbesserte molekularbiologische prozessanlage
WO2008151207A3 (en) Expression quantification using mass spectrometry
WO2011146521A3 (en) A general mass spectrometry assay using continuously eluting co-fractionating reporters of mass spectrometry detection efficiency
ATE455302T1 (de) Komplexformierungs- und -trennungsverfahren
WO2004083819A3 (en) Molecular forensic specimen marker
BRPI0908618A2 (pt) método para a coleta, processamento e análise de um ácido nucléico alvo a partir de uma amostra, kit que fornece materiais e um recipiente para preparar as amostras e para detectar ou identificar um dna ou rna alvo diagnóstico e sistema diagnóstico para a preparação da amostra e detecção ou identificação de um ácido nucléico alvo
WO2005010485A3 (en) Methods for detecting and analyzing n-glycolylneuraminic acid (neu5gc) in biological materials
WO2008020823A3 (en) Cooperative reporter systems, components, and methods for analyte detection
EP2176432A4 (en) METHOD FOR DETECTION OF SICKNESS MARKERS
EP1930445A4 (en) KIT AND METHOD FOR DETECTING UROTHEL CARCINOMA
Treitz et al. Combination of a SAW-biosensor with MALDI mass spectrometric analysis
WO2007030373A3 (en) Method for in situ hybridization analysis

Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B09X Republication of the decision to grant [chapter 9.1.3 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]
B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 28/04/2006, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO