BRPI0706801B8 - método de purificação de cardiomiócitos ou cardiomiócitos programados derivado de células-tronco ou fetos - Google Patents
método de purificação de cardiomiócitos ou cardiomiócitos programados derivado de células-tronco ou fetosInfo
- Publication number
- BRPI0706801B8 BRPI0706801B8 BRPI0706801A BRPI0706801A BRPI0706801B8 BR PI0706801 B8 BRPI0706801 B8 BR PI0706801B8 BR PI0706801 A BRPI0706801 A BR PI0706801A BR PI0706801 A BRPI0706801 A BR PI0706801A BR PI0706801 B8 BRPI0706801 B8 BR PI0706801B8
- Authority
- BR
- Brazil
- Prior art keywords
- cardiomyocytes
- condition
- stem cells
- fetuses
- derived
- Prior art date
Links
- 210000004413 cardiac myocyte Anatomy 0.000 title abstract 10
- 238000000034 method Methods 0.000 title abstract 5
- 210000003754 fetus Anatomy 0.000 title abstract 4
- 238000000746 purification Methods 0.000 title abstract 3
- 210000000130 stem cell Anatomy 0.000 title abstract 3
- LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 abstract 2
- 210000001671 embryonic stem cell Anatomy 0.000 abstract 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 abstract 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 abstract 1
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 239000013543 active substance Substances 0.000 abstract 1
- 210000004504 adult stem cell Anatomy 0.000 abstract 1
- 235000003704 aspartic acid Nutrition 0.000 abstract 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 abstract 1
- 229910052791 calcium Inorganic materials 0.000 abstract 1
- 239000011575 calcium Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000012239 gene modification Methods 0.000 abstract 1
- 230000005017 genetic modification Effects 0.000 abstract 1
- 235000013617 genetically modified food Nutrition 0.000 abstract 1
- 239000008103 glucose Substances 0.000 abstract 1
- 235000013922 glutamic acid Nutrition 0.000 abstract 1
- 239000004220 glutamic acid Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 235000014655 lactic acid Nutrition 0.000 abstract 1
- 239000004310 lactic acid Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 229940107700 pyruvic acid Drugs 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0657—Cardiomyocytes; Heart cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/02—Atmosphere, e.g. low oxygen conditions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
- C12N2500/14—Calcium; Ca chelators; Calcitonin
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
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- C12N2500/32—Amino acids
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/34—Sugars
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- C12N2500/30—Organic components
- C12N2500/38—Vitamins
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/44—Thiols, e.g. mercaptoethanol
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- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/235—Leukemia inhibitory factor [LIF]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Rheumatology (AREA)
- Cardiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
método de purificação de cardiomiõcitos ou cardiomiócitos programados derivado de células- tronco ou fetos a presente invenção refere-se ao desenvolvimento de um método para purificar cardiomiácitos com alto grau de purificação e com alto rendimento a partir de uma mistura celular compreendendo cardiomiócitos derivados de fetos e de células-tronco usando várias características as quais não foram anteriormente esperadas para serem usadas para a purificação de cardiomiócitos, ou as quais são recentemente descobertas, em que o referido método é executado sem sofrer qualquer modificação genética ou sem adicionar quaisquer proteínas ou agentes biologicamente ativos especiais. os inventores da presente invenção descobriram que os cardio-miócitos foram altamente selecionados e purificados de forma eficaz pelo cultivo de cardiomiácitos derivados de células-tronco embrionárias no meio de cultura sob uma condição selecionada de uma condição selecionada com pouco soro, uma condição suplementada com pouca glicose, uma condição nutricional baixa, uma condição com pouco cálcio, uma condição com ph brandamente ácido, uma condição suplementada com ácido lático, uma condição suplementada com ácido aspártico/ácido glutâmico e/ou uma condição suplementada com ácido pirúvico. os inventores da presente invenção ainda descobriram que o método inventado acima, em relação às células-tronco embrionárias, foi aplicável para selecionar e purificar cardiomiácitos derivados de fetos ou de células-tronco adultas.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2006023770 | 2006-01-31 | ||
| JP2006-023770 | 2006-01-31 | ||
| PCT/JP2007/051563 WO2007088874A1 (ja) | 2006-01-31 | 2007-01-31 | 幹細胞及び胎児由来の心筋細胞及び予定心筋細胞の精製方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BRPI0706801A2 BRPI0706801A2 (pt) | 2011-04-05 |
| BRPI0706801B1 BRPI0706801B1 (pt) | 2020-04-07 |
| BRPI0706801B8 true BRPI0706801B8 (pt) | 2021-05-25 |
Family
ID=38327445
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0706801A BRPI0706801B8 (pt) | 2006-01-31 | 2007-01-31 | método de purificação de cardiomiócitos ou cardiomiócitos programados derivado de células-tronco ou fetos |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US9115342B2 (pt) |
| EP (2) | EP2468851A1 (pt) |
| JP (2) | JP5312804B2 (pt) |
| KR (2) | KR101455144B1 (pt) |
| CN (2) | CN101374942A (pt) |
| AU (1) | AU2007210580B2 (pt) |
| BR (1) | BRPI0706801B8 (pt) |
| CA (1) | CA2640644C (pt) |
| IL (1) | IL193095A (pt) |
| RU (1) | RU2426784C2 (pt) |
| SG (1) | SG169359A1 (pt) |
| WO (1) | WO2007088874A1 (pt) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3354723B1 (en) | 2005-08-29 | 2023-12-13 | Technion Research & Development Foundation Ltd. | Media for culturing stem cells |
| JP5312804B2 (ja) * | 2006-01-31 | 2013-10-09 | 第一三共株式会社 | 幹細胞及び胎児由来の心筋細胞及び予定心筋細胞の精製方法 |
| EP3441459B1 (en) | 2006-08-02 | 2021-03-17 | Technion Research & Development Foundation Limited | Methods of expanding embryonic stem cells in a suspension culture |
| CN102449139B (zh) | 2009-03-30 | 2016-08-24 | 第一三共株式会社 | 对多能性干细胞和心肌细胞以外的分化细胞诱导细胞死亡的方法 |
| ES2932664T3 (es) | 2009-11-12 | 2023-01-23 | Technion Res & Dev Foundation | Medios de cultivo, cultivos celulares y métodos de cultivo de células madre pluripotentes en estado no diferenciado |
| AU2011268056B2 (en) | 2010-06-18 | 2014-04-24 | Cellular Dynamics International, Inc. | Cardiomyocyte medium with dialyzed serum |
| KR101364965B1 (ko) * | 2010-10-06 | 2014-02-18 | (주)차바이오앤디오스텍 | 인간 배아줄기세포 유래 심근세포의 배양 및 정제 방법 |
| JP5840855B2 (ja) | 2011-03-30 | 2016-01-06 | 学校法人東京女子医科大学 | 胚性幹細胞から心筋シートを製造する方法 |
| CA2841985C (en) * | 2011-07-29 | 2019-09-17 | Cellular Dynamics International, Inc. | Metabolic maturation in stem cell-derived tissue cells |
| ES2523903B1 (es) * | 2013-04-30 | 2015-09-09 | Fundación Pública Andaluza Progreso Y Salud | Método de obtención de datos útiles para el diagnóstico diferencial de la fibrosis hepática |
| CN105039399A (zh) * | 2014-04-23 | 2015-11-11 | 复旦大学 | 多能干细胞-遗传性心肌病心肌细胞及其制备方法 |
| BR112016030723B8 (pt) * | 2014-07-16 | 2024-02-27 | Heartseed Inc | Meios de cultura de células, método para indução da morte celular de células-tronco indiferenciadas e métodos para seleção de cardiomiócitos |
| WO2016043168A1 (ja) | 2014-09-16 | 2016-03-24 | 国立大学法人大阪大学 | 多能性幹細胞由来心筋細胞集団の製造方法 |
| KR102639313B1 (ko) * | 2014-11-07 | 2024-02-20 | 고꾸리쯔 다이가꾸 호우징 오사까 다이가꾸 | 미분화 세포가 제거된 분화 유도 세포집단, 이의 용도 및 이의 제조 방법 |
| JP6622007B2 (ja) * | 2015-05-29 | 2019-12-18 | オリンパス株式会社 | 細胞評価方法 |
| JP7089484B2 (ja) | 2016-06-01 | 2022-06-22 | ミルテニイ ビオテック ベー．ファー． ウント コー．カーゲー | ヒト多能性幹細胞由来の心筋細胞および心筋細胞サブポピュレーションの生成、同定ならびに単離のための方法 |
| CN106244527B (zh) * | 2016-08-29 | 2019-10-08 | 广东依浦赛生物科技有限公司 | 人源iPS干细胞体外定向分化为心肌细胞的试剂盒及方法 |
| WO2018074457A1 (ja) * | 2016-10-17 | 2018-04-26 | 学校法人慶應義塾 | 未分化幹細胞除去剤、及び未分化幹細胞除去方法 |
| SG11201805456WA (en) | 2016-11-01 | 2019-05-30 | Terumo Corp | Modification method for sheet-shaped cell culture |
| CN106754663A (zh) * | 2016-11-30 | 2017-05-31 | 中国人民解放军第三军医大学第三附属医院 | 促进成年哺乳动物心肌细胞存活的体外培养方法及监测成年哺乳动物心肌细胞增殖的方法 |
| JP2018093823A (ja) | 2016-12-15 | 2018-06-21 | Ｈｅａｒｔｓｅｅｄ株式会社 | 未分化幹細胞除去剤及び未分化幹細胞除去方法 |
| KR101879517B1 (ko) * | 2016-12-27 | 2018-07-17 | 전남대학교병원 | 중간엽 줄기세포를 심근세포로 분화시키는 방법 |
| CN110506109B (zh) | 2017-06-05 | 2024-03-12 | 泰尔茂株式会社 | 细胞培养物的制造方法 |
| CN107828721A (zh) * | 2017-11-10 | 2018-03-23 | 山东农业大学 | 一种鸡胚心肌细胞的培养及免疫组织化学鉴定方法 |
| WO2020067435A1 (ja) * | 2018-09-27 | 2020-04-02 | 国立大学法人大阪大学 | 多能性幹細胞由来細胞のシート化方法 |
| EP3739040B1 (en) | 2018-09-27 | 2023-07-05 | Osaka University | Sheeting method for pluripotent stem cell-derived cells |
| WO2020067436A1 (ja) * | 2018-09-27 | 2020-04-02 | 国立大学法人大阪大学 | 多能性幹細胞由来細胞の移植片形成方法 |
| CN110904024A (zh) * | 2019-11-13 | 2020-03-24 | 武汉济源高科技有限公司 | 一种悬浮细胞培养中去除死细胞的方法 |
| CN111621470B (zh) * | 2020-06-08 | 2023-06-27 | 广东源心再生医学有限公司 | 一种高效低毒的心肌纯化培养基及方法 |
| CN114591895B (zh) * | 2022-04-12 | 2025-07-22 | 澳门大学 | 一种诱导干细胞分化为心肌细胞的方法及其培养基组合 |
| EP4551690A1 (en) | 2022-07-05 | 2025-05-14 | Repairon GmbH | Differentiation of ipscs in bioreactors |
| WO2024248109A1 (ja) | 2023-05-30 | 2024-12-05 | Ｈｅａｒｔｓｅｅｄ株式会社 | 心筋細胞群、医薬組成物、心筋細胞群の製造方法、及び心筋球 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL47012C (pt) | 1933-03-23 | |||
| GB9903804D0 (en) | 1999-02-20 | 1999-04-14 | Univ Sheffield | Pluripotential cells-2 |
| AU784618B2 (en) | 1999-12-28 | 2006-05-18 | Kyowa Hakko Kogyo Co. Ltd. | Cells capable of differentiating into heart muscle cells |
| IL156234A0 (en) * | 2000-11-30 | 2004-01-04 | Stemron Inc | Isolated homozygous stem cells, differentiated cells derived therefrom, and materials and methods for making and using same |
| US7732199B2 (en) * | 2001-07-12 | 2010-06-08 | Geron Corporation | Process for making transplantable cardiomyocytes from human embryonic stem cells |
| WO2003006950A2 (en) * | 2001-07-12 | 2003-01-23 | Geron Corporation | Cells of the cardiomyocyte lineage produced from human pluripotent stem cells |
| US8158421B2 (en) * | 2004-01-14 | 2012-04-17 | Es Cell International Pte Ltd | Cardiomyocyte differentiation |
| AU2005224670B2 (en) | 2004-03-19 | 2010-11-11 | Asterias Biotherapeutics, Inc. | Method for making high purity cardiomyocyte preparations suitable for regenerative medicine |
| WO2006066320A1 (en) * | 2004-12-22 | 2006-06-29 | Es Cell International Pte Ltd | Differentiation of human embryonic stem cells and cardiomyocytes and cardiomyocyte progenitors derived therefrom |
| JP5312804B2 (ja) * | 2006-01-31 | 2013-10-09 | 第一三共株式会社 | 幹細胞及び胎児由来の心筋細胞及び予定心筋細胞の精製方法 |
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2007
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- 2007-01-31 EP EP20120160317 patent/EP2468851A1/en not_active Withdrawn
- 2007-01-31 AU AU2007210580A patent/AU2007210580B2/en active Active
- 2007-01-31 EP EP07713734.7A patent/EP1983042B1/en active Active
- 2007-01-31 BR BRPI0706801A patent/BRPI0706801B8/pt active IP Right Grant
- 2007-01-31 US US12/162,684 patent/US9115342B2/en active Active
- 2007-01-31 CA CA2640644A patent/CA2640644C/en active Active
- 2007-01-31 SG SG201100658-2A patent/SG169359A1/en unknown
- 2007-01-31 CN CNA200780003957XA patent/CN101374942A/zh active Pending
- 2007-01-31 WO PCT/JP2007/051563 patent/WO2007088874A1/ja not_active Ceased
- 2007-01-31 KR KR20127027002A patent/KR101455144B1/ko active Active
- 2007-01-31 KR KR1020087020152A patent/KR101348325B1/ko active Active
- 2007-01-31 RU RU2008135313A patent/RU2426784C2/ru active
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Also Published As
| Publication number | Publication date |
|---|---|
| US20090275132A1 (en) | 2009-11-05 |
| CA2640644C (en) | 2013-11-26 |
| CN103409368A (zh) | 2013-11-27 |
| BRPI0706801A2 (pt) | 2011-04-05 |
| US9115342B2 (en) | 2015-08-25 |
| KR101348325B1 (ko) | 2014-01-08 |
| KR20120123608A (ko) | 2012-11-08 |
| EP1983042A1 (en) | 2008-10-22 |
| CN103409368B (zh) | 2016-05-25 |
| KR101455144B1 (ko) | 2014-10-27 |
| JP5312804B2 (ja) | 2013-10-09 |
| JPWO2007088874A1 (ja) | 2009-06-25 |
| CA2640644A1 (en) | 2007-08-09 |
| AU2007210580A1 (en) | 2007-08-09 |
| AU2007210580B2 (en) | 2012-10-11 |
| IL193095A0 (en) | 2009-02-11 |
| EP1983042A4 (en) | 2009-06-17 |
| BRPI0706801B1 (pt) | 2020-04-07 |
| EP2468851A1 (en) | 2012-06-27 |
| EP1983042B1 (en) | 2017-03-15 |
| JP5756145B2 (ja) | 2015-07-29 |
| RU2426784C2 (ru) | 2011-08-20 |
| CN101374942A (zh) | 2009-02-25 |
| RU2008135313A (ru) | 2010-03-20 |
| WO2007088874A1 (ja) | 2007-08-09 |
| KR20080097197A (ko) | 2008-11-04 |
| SG169359A1 (en) | 2011-03-30 |
| JP2013143968A (ja) | 2013-07-25 |
| IL193095A (en) | 2015-06-30 |
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