BRPI0811490A2 - Método colorimétrico e estojo de detecção de sequências específicas de ácidos nucléicos através de nanopartículas metálicas funcionalizadas com oligonucleotídos modificados. - Google Patents

Método colorimétrico e estojo de detecção de sequências específicas de ácidos nucléicos através de nanopartículas metálicas funcionalizadas com oligonucleotídos modificados.

Info

Publication number
BRPI0811490A2
BRPI0811490A2 BRPI0811490-0A2A BRPI0811490A BRPI0811490A2 BR PI0811490 A2 BRPI0811490 A2 BR PI0811490A2 BR PI0811490 A BRPI0811490 A BR PI0811490A BR PI0811490 A2 BRPI0811490 A2 BR PI0811490A2
Authority
BR
Brazil
Prior art keywords
oligonucleotids
nanoparticules
modified
detection
nucleic acid
Prior art date
Application number
BRPI0811490-0A2A
Other languages
English (en)
Inventor
Jose Ricardo Ramos Franco Tavares
Miguel Ribeiro Viana Baptista
Goncalo Maria Reimao Pinto De Franca Doria
Alcino Orfeu De Leao Flores
Original Assignee
Stab Vida Investigacao E Servicos Em Ciencias Biolog Lda
Univ Nova De Lisboa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stab Vida Investigacao E Servicos Em Ciencias Biolog Lda, Univ Nova De Lisboa filed Critical Stab Vida Investigacao E Servicos Em Ciencias Biolog Lda
Publication of BRPI0811490A2 publication Critical patent/BRPI0811490A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BRPI0811490-0A2A 2007-05-04 2008-05-02 Método colorimétrico e estojo de detecção de sequências específicas de ácidos nucléicos através de nanopartículas metálicas funcionalizadas com oligonucleotídos modificados. BRPI0811490A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PT103730A PT103730A (pt) 2007-05-04 2007-05-04 Método colorimétrico e estojo de detecção de sequências específicas de ácidos nucleicos através de nanopartículas metálicas funcionalizadas com oligonucleótidos modificados
PCT/IB2008/051708 WO2008135929A2 (en) 2007-05-04 2008-05-02 Colorimetric method and kit for the detection of specific nucleic acid sequences using metal nanoparticles functionalized with modified oligonucleotides

Publications (1)

Publication Number Publication Date
BRPI0811490A2 true BRPI0811490A2 (pt) 2014-11-04

Family

ID=39761016

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0811490-0A2A BRPI0811490A2 (pt) 2007-05-04 2008-05-02 Método colorimétrico e estojo de detecção de sequências específicas de ácidos nucléicos através de nanopartículas metálicas funcionalizadas com oligonucleotídos modificados.

Country Status (5)

Country Link
US (1) US20100075335A1 (pt)
EP (1) EP2145023A2 (pt)
BR (1) BRPI0811490A2 (pt)
PT (1) PT103730A (pt)
WO (1) WO2008135929A2 (pt)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101048429B1 (ko) * 2009-09-09 2011-07-11 한국생명공학연구원 바이오칩을 이용한 표적 물질 검출 및 정량 방법
KR101329300B1 (ko) 2010-10-14 2013-11-14 한국과학기술원 과산화효소 활성을 가지는 자성 나노입자 기반의 발색반응 현상을 이용한 핵산 및 생체물질 검출방법
CN102041312B (zh) * 2010-10-15 2012-12-26 中国科学院上海微系统与信息技术研究所 利用核酸酶反应进行dna单碱基突变颜色检测的方法
EP2631300A1 (en) * 2012-02-23 2013-08-28 Biosure R & T Cell Co. Detection of leishmania
DE102012203964B3 (de) 2012-03-14 2013-02-28 GNA Biosolultions GmbH Verfahren und Kit zur Detektion von Nukleinsäuren
US20140134609A1 (en) * 2012-08-17 2014-05-15 Agency For Science, Technology And Research Enzymatic metal nanoparticle sensor for detecting dna binders
WO2014065753A1 (en) * 2012-10-23 2014-05-01 Dso National Laboratories Direct visual detection of nucleic acid using gold nanoparticles
DE102015010069B3 (de) 2015-08-03 2016-07-21 Gna Biosolutions Gmbh Verfahren zum Nachweis einer Nukleinsäure
CN105527240A (zh) * 2016-01-21 2016-04-27 南昌大学 一种基于银纳米可视化检测镉离子的方法
US10400268B2 (en) 2016-11-02 2019-09-03 The Queen's University Of Belfast Methods and kits for the detection of DNA
CN106680232A (zh) * 2017-03-21 2017-05-17 中国科学院长春光学精密机械与物理研究所 用于检测水溶液中Hg2+的试剂组合物、试剂溶液及其制备方法和检测方法
KR20190011117A (ko) * 2017-07-24 2019-02-01 사회복지법인 삼성생명공익재단 금 나노입자의 응집을 이용한 유전자의 변이를 검출하는 방법
WO2019162465A1 (en) * 2018-02-22 2019-08-29 Nano4 Global, Lda Method for detecting flaviviridae
ES3056904T3 (en) * 2019-04-25 2026-02-25 Agilent Technologies Inc Hybridization compositions and methods for making and using compositions
CN113828771B (zh) * 2021-09-18 2023-06-27 福州大学 一种氟取代核酸修饰金颗粒的制备方法
CN116083535A (zh) * 2022-10-08 2023-05-09 上海橙康生物科技有限公司 一种实现多微生物检测的金纳米检测方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07227299A (ja) * 1994-02-14 1995-08-29 Kyoto Daiichi Kagaku:Kk Ｄｎａの特定塩基配列の検出方法及びその装置
US6582921B2 (en) * 1996-07-29 2003-06-24 Nanosphere, Inc. Nanoparticles having oligonucleotides attached thereto and uses thereof
US20040185462A1 (en) * 1999-08-06 2004-09-23 Tum Gene, Inc. Method of and detecting apparatus and detecting chip for single base substitution SNP and point mutation of genes
US20030013096A1 (en) * 2000-03-16 2003-01-16 Ichiro Yamashita Nucleotide detector, process for producing the same and process for morming forming fine particle membrane
US6972173B2 (en) * 2002-03-14 2005-12-06 Intel Corporation Methods to increase nucleotide signals by raman scattering
US20050208592A1 (en) * 2004-03-17 2005-09-22 National Research Council Of Canada Method and apparatus for the detection of microorganisms
US20060222595A1 (en) * 2005-03-31 2006-10-05 Priyabrata Mukherjee Nanoparticles for therapeutic and diagnostic applications
US20100035243A1 (en) * 2006-07-10 2010-02-11 Nanosphere, Inc. Ultra-sensitive detection of analytes

Also Published As

Publication number Publication date
WO2008135929A3 (en) 2008-12-31
PT103730A (pt) 2008-11-04
US20100075335A1 (en) 2010-03-25
WO2008135929A2 (en) 2008-11-13
EP2145023A2 (en) 2010-01-20

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Legal Events

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]