BRPI0905853A8 - processo para a produção de proteínas recombinantes, melhorando a incorporação de fosfato. - Google Patents

processo para a produção de proteínas recombinantes, melhorando a incorporação de fosfato.

Info

Publication number
BRPI0905853A8
BRPI0905853A8 BRPI0905853A BRPI0905853A BRPI0905853A8 BR PI0905853 A8 BRPI0905853 A8 BR PI0905853A8 BR PI0905853 A BRPI0905853 A BR PI0905853A BR PI0905853 A BRPI0905853 A BR PI0905853A BR PI0905853 A8 BRPI0905853 A8 BR PI0905853A8
Authority
BR
Brazil
Prior art keywords
recombinant proteins
producing recombinant
phosphate uptake
fermentation media
improving phosphate
Prior art date
Application number
BRPI0905853A
Other languages
English (en)
Inventor
Goel Anuj
Kumar Bimal
Ramegowda Naveen Kumar Chittnalli
Iyer Harish
Kumar Garg Mayank
Babuappa Patale Mukesh
Tiwari Sanjay
Garg Saurabh
Joshi Sulekha
Original Assignee
Biocon Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocon Ltd filed Critical Biocon Ltd
Publication of BRPI0905853A2 publication Critical patent/BRPI0905853A2/pt
Publication of BRPI0905853A8 publication Critical patent/BRPI0905853A8/pt
Publication of BRPI0905853B1 publication Critical patent/BRPI0905853B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

meios de fermentação e processos destes. a presente invenção demonstra a utilidade de amidas de ácido carbônico como ureia ou seus derivados, carbamatos, carbodiimidas e tiocarbamidas como suplementos nitrogenosos em meios de fermentação para produção de proteínas recombinantes para atingir taxas de bioconversão elevadas e peptídeos semelhantes à insulina e análogos de insulina, exendina e enzimas como lipase usando sistemas de expressão fúngica induzíveis por metanol, como pichia.
BRPI0905853-2A 2008-02-06 2009-02-05 Processo para a produção de proteínas recombinantes, melhorando a incorporação de fosfato. BRPI0905853B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
IN310CH2008 2008-02-06
IN00310/CHE/2008 2008-02-06
IN00086/CHE/2009 2009-01-12
IN86CH2009 2009-01-12
PCT/IN2009/000078 WO2009113099A2 (en) 2008-02-06 2009-02-05 Fermentation medias and processes thereof

Publications (3)

Publication Number Publication Date
BRPI0905853A2 BRPI0905853A2 (pt) 2015-06-30
BRPI0905853A8 true BRPI0905853A8 (pt) 2019-01-29
BRPI0905853B1 BRPI0905853B1 (pt) 2019-03-26

Family

ID=41065638

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0905853-2A BRPI0905853B1 (pt) 2008-02-06 2009-02-05 Processo para a produção de proteínas recombinantes, melhorando a incorporação de fosfato.

Country Status (12)

Country Link
US (2) US8927256B2 (pt)
EP (2) EP2565272A3 (pt)
JP (1) JP5587795B2 (pt)
KR (2) KR101492011B1 (pt)
CN (2) CN101983242B (pt)
BR (1) BRPI0905853B1 (pt)
IL (2) IL207402A (pt)
MX (2) MX2010008654A (pt)
MY (1) MY161108A (pt)
RU (3) RU2491345C2 (pt)
SG (1) SG188126A1 (pt)
WO (1) WO2009113099A2 (pt)

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JP2016506401A (ja) 2012-12-21 2016-03-03 サノフイ 二重ｇｌｐ１／ｇｉｐまたは三方ｇｌｐ１／ｇｉｐ／グルカゴンアゴニストとしてのエキセンジン−４誘導体
CN103911298B (zh) * 2012-12-31 2016-08-10 中粮营养健康研究院有限公司 培养基组合物和培养基及对菌株进行活菌计数的方法
TW201609796A (zh) 2013-12-13 2016-03-16 賽諾菲公司 非醯化之艾塞那肽－４（ｅｘｅｎｄｉｎ－４）胜肽類似物
EP3080150B1 (en) 2013-12-13 2018-08-01 Sanofi Exendin-4 peptide analogues as dual glp-1/gip receptor agonists
WO2015086733A1 (en) 2013-12-13 2015-06-18 Sanofi Dual glp-1/glucagon receptor agonists
TW201609799A (zh) 2013-12-13 2016-03-16 賽諾菲公司 雙重ｇｌｐ－１／ｇｉｐ受體促效劑
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TW201706291A (zh) 2015-07-10 2017-02-16 賽諾菲公司 作為選擇性肽雙重ｇｌｐ－１／升糖素受體促效劑之新毒蜥外泌肽（ｅｘｅｎｄｉｎ－４）衍生物
CN108350471A (zh) * 2015-10-20 2018-07-31 巴克曼实验室国际公司 增强用于发酵生物产品生产的酵母生长的方法及其营养组合物
EP3405785B1 (en) * 2016-01-23 2020-04-29 Biocon Limited Bio-analytical method for insulin analogues
CN107022591B (zh) * 2017-05-11 2018-06-12 宜昌东阳光长江药业股份有限公司 一种提高胰岛素及其类似物前体表达的毕赤酵母发酵方法
WO2018216978A1 (en) * 2017-05-22 2018-11-29 Cj Healthcare Corporation Cell culture method at low temperature applicable to mass production of protein
CN107988292B (zh) * 2018-01-18 2023-12-26 江苏江山聚源生物技术有限公司 提高重组人源胶原蛋白稳定性的发酵工艺
CN111411049B (zh) * 2020-04-15 2022-11-22 北京惠之衡生物科技有限公司 一种提高胰岛素前体表达的毕赤酵母的发酵培养基及发酵方法
CN111996136A (zh) * 2020-07-24 2020-11-27 北大方正集团有限公司 一种荧光假单孢杆菌发酵培养基、培养方法与应用
CN114507609B (zh) * 2020-12-22 2023-04-18 益海嘉里(连云港)生物科技有限公司 一种提高磷脂酶c蛋白产量的方法
CN117467716A (zh) * 2022-07-22 2024-01-30 上海医药工业研究院有限公司 一种提高洛伐他汀发酵单位的发酵培养基及其应用

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Also Published As

Publication number Publication date
WO2009113099A3 (en) 2009-11-05
MX349413B (es) 2017-07-19
KR101492011B1 (ko) 2015-02-10
CN103642843A (zh) 2014-03-19
US20100317056A1 (en) 2010-12-16
RU2010137009A (ru) 2012-03-20
WO2009113099A2 (en) 2009-09-17
CN101983242B (zh) 2015-12-16
RU2011153811A (ru) 2013-07-10
US9255285B2 (en) 2016-02-09
EP2565272A2 (en) 2013-03-06
IL221379A (en) 2013-08-29
US20140141467A1 (en) 2014-05-22
EP2565272A3 (en) 2013-07-03
KR20120066068A (ko) 2012-06-21
SG188126A1 (en) 2013-03-28
CN103642843B (zh) 2017-03-01
EP2247737A4 (en) 2012-03-14
RU2012145969A (ru) 2014-05-10
BRPI0905853A2 (pt) 2015-06-30
IL221379A0 (en) 2012-10-31
JP5587795B2 (ja) 2014-09-10
JP2011510677A (ja) 2011-04-07
BRPI0905853B1 (pt) 2019-03-26
RU2491345C2 (ru) 2013-08-27
IL207402A (en) 2013-07-31
MX2010008654A (es) 2010-10-06
IL207402A0 (en) 2010-12-30
RU2556120C2 (ru) 2015-07-10
MY161108A (en) 2017-04-14
US8927256B2 (en) 2015-01-06
RU2595380C2 (ru) 2016-08-27
KR101283734B1 (ko) 2013-07-08
EP2247737B1 (en) 2018-06-20
KR20100118592A (ko) 2010-11-05
EP2247737A2 (en) 2010-11-10
CN101983242A (zh) 2011-03-02

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Legal Events

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B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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