BRPI0909611A2 - ''polinucleotídeo isolado, construção de dna recombinante, célula, oleaginosa transgênica, método de aumento do teor total de ácido graxo de oleginosas, método de aumento do teor total de ácido graxo de uma célula de oleaginosa, fragmento de ácido nucleico, fragmento de ácido nucleico isolado e célula fúngica'' - Google Patents

''polinucleotídeo isolado, construção de dna recombinante, célula, oleaginosa transgênica, método de aumento do teor total de ácido graxo de oleginosas, método de aumento do teor total de ácido graxo de uma célula de oleaginosa, fragmento de ácido nucleico, fragmento de ácido nucleico isolado e célula fúngica''

Info

Publication number
BRPI0909611A2
BRPI0909611A2 BRPI0909611A BRPI0909611A BRPI0909611A2 BR PI0909611 A2 BRPI0909611 A2 BR PI0909611A2 BR PI0909611 A BRPI0909611 A BR PI0909611A BR PI0909611 A BRPI0909611 A BR PI0909611A BR PI0909611 A2 BRPI0909611 A2 BR PI0909611A2
Authority
BR
Brazil
Prior art keywords
cell
increasing
total fatty
fatty acid
oleaginous
Prior art date
Application number
BRPI0909611A
Other languages
English (en)
Inventor
Shen Bo
Li Changjiang
Bermudez Ericka
Glenn Damude Howard
Roesler Keith
Meyer Knut
C Tarczynski Mitchell
Original Assignee
Du Pont
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Pioneer Hi Bred Int filed Critical Du Pont
Publication of BRPI0909611A2 publication Critical patent/BRPI0909611A2/pt
Publication of BRPI0909611B1 publication Critical patent/BRPI0909611B1/pt
Publication of BRPI0909611B8 publication Critical patent/BRPI0909611B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • Mycology (AREA)
  • Agronomy & Crop Science (AREA)
  • Fats And Perfumes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BRPI0909611A 2008-05-23 2009-05-22 Método de aumento do teor total de ácido graxo de uma célula de oleaginosa, ácido nucleico recombinante, construção de dna recombinante e método de produção de uma semente oleaginosa BRPI0909611B8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5557908P 2008-05-23 2008-05-23
PCT/US2009/044926 WO2009143397A2 (en) 2008-05-23 2009-05-22 Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants

Publications (3)

Publication Number Publication Date
BRPI0909611A2 true BRPI0909611A2 (pt) 2017-05-30
BRPI0909611B1 BRPI0909611B1 (pt) 2018-09-11
BRPI0909611B8 BRPI0909611B8 (pt) 2022-12-06

Family

ID=41110732

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0909611A BRPI0909611B8 (pt) 2008-05-23 2009-05-22 Método de aumento do teor total de ácido graxo de uma célula de oleaginosa, ácido nucleico recombinante, construção de dna recombinante e método de produção de uma semente oleaginosa

Country Status (6)

Country Link
US (5) US8101819B2 (pt)
EP (2) EP2297310A2 (pt)
AU (2) AU2009248931A1 (pt)
BR (1) BRPI0909611B8 (pt)
CA (2) CA2990650C (pt)
WO (1) WO2009143397A2 (pt)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008006171A1 (en) 2006-07-14 2008-01-17 Commonwealth Scientific And Industrial Research Organisation Altering the fatty acid composition of rice
DE102006054481A1 (de) * 2006-11-18 2008-05-21 Eppendorf Ag Vakuumkonzentrator und Verfahren zur Vakuumkonzentration
WO2009109051A1 (en) * 2008-03-07 2009-09-11 The Governors Of The University Of Alberta Diacyglycerol acyltransferases from flax
AU2009248932B2 (en) 2008-05-23 2015-08-13 E. I. Du Pont De Nemours And Company DGAT genes from yarrowia lipolytica combined with plastidic phosphoglucomutase down regulation for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
EP2297310A2 (en) * 2008-05-23 2011-03-23 E. I. du Pont de Nemours and Company Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
EP2620502A3 (en) 2008-05-23 2013-09-25 E. I. du Pont de Nemours and Company DGAT genes from oleaginous organisms for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
CN102202498B (zh) 2008-07-21 2016-09-07 澳大利亚联邦科学与工业研究组织 改良的棉籽油及应用
WO2010009500A1 (en) 2008-07-21 2010-01-28 Commonwealth Scientific And Industrial Research Organisation Improved vegetable oils and uses therefor
CA2804025C (en) 2010-06-28 2023-02-21 Commonwealth Scientific And Industrial Research Organisation Methods of producing lipids
WO2012059925A2 (en) * 2010-11-04 2012-05-10 Ben-Gurion University Of The Negev Research And Development Authority Acyl-coa: diacylglycerol acyltransferase 1-like gene (ptdgat1) and uses thereof
CA2860220C (en) 2011-12-22 2021-07-13 E. I. Du Pont De Nemours And Company Use of the soybean or medicago truncatula sucrose synthase promoter to increase plant seed oil content
US8809026B2 (en) 2011-12-27 2014-08-19 Commonwealth Scientific And Industrial Research Organisation Processes for producing lipids
US11639507B2 (en) 2011-12-27 2023-05-02 Commonwealth Scientific And Industrial Research Organisation Processes for producing lipids
PH12014501474B1 (en) 2011-12-27 2024-01-19 Nuseed Global Innovation Ltd Processes for producing lipids
BR112014018009A2 (pt) 2012-01-23 2018-06-26 Du Pont sequência de ácido nucleico isolada, construção recombinante, célula vegetal, método para reduzir a expressão de pelo menos um gene biossintético de ácido graxo vegetal, método para reduzir a expressão de dois ou mais genes biossintéticos de ácido graxo vegetal e planta ou semente transgênica
HK1207938A1 (en) 2012-04-25 2016-02-19 Commonwealth Scientific And Industrial Research Organisation High oleic acid oils
WO2014049580A2 (en) * 2012-09-28 2014-04-03 Jawaharlal Nehru Centre For Advanced Scientific Research Assay to monitor autophagy, a method and kit thereof
BR112015009303A2 (pt) * 2012-10-30 2017-08-22 Agresearch Ltd Novos polipeptídeos, polinucleotídeos de aciltransferase, e métodos de uso
US9896694B2 (en) * 2012-10-30 2018-02-20 Agresearch Limited Acyltransferase polynucleotides, polypeptides and methods of use
CA2889800A1 (en) 2012-10-30 2014-05-08 Agresearch Limited Enhanced acyltransferase polynucleotides, polypeptides, and methods of use
EP2935590A4 (en) 2012-12-21 2016-09-28 Nz Inst Plant & Food Res Ltd REGULATION OF GENE EXPRESSION
US20140289908A1 (en) * 2013-03-25 2014-09-25 The Governors Of The University Of Alberta Gene combinations for producing punicic acid in transgenic plants
WO2015017510A1 (en) * 2013-07-31 2015-02-05 E. I. Du Pont De Nemours And Company Modification of soybean seed composition to enhance feed, food and other industrial applications of soybean products
AR100159A1 (es) 2014-04-22 2016-09-14 Du Pont Genes de anhidrasa carbónica plastídica para el incremento de aceite en semillas con expresión incrementada de dgat
MY204560A (en) 2014-07-07 2024-09-04 Nuseed Global Innovation Ltd Process for producing industrial products from plant lipids
WO2018039740A1 (en) 2016-09-02 2018-03-08 Commonwealth Scientific And Industrial Research Organisation Plants with modified traits
BR112019018200B1 (pt) 2017-03-03 2024-02-20 Pioneer Hi-Bred International, Inc Método para medir a quantidade de um sucrosil-oligossacarídeo,método para medir estaquiose, método para processar sementes de soja geneticamente modificadas
CA3099278A1 (en) 2018-06-01 2019-12-05 Pioneer Hi-Bred International, Inc. Modified seed oil content by gene editing
WO2022246387A1 (en) * 2021-05-17 2022-11-24 Pioneer Hi-Bred International, Inc. Modified seed oil content in plants

Family Cites Families (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1987A (en) 1841-02-20 austin
DE1492986C2 (de) 1964-05-21 1979-05-17 Archer Daniels Midland Co, Minneapolis, Mina (V.StA.) Verfahren zur Herstellung von Proteinnahrungsmitteln mit Fleischcharakter
US3897574A (en) 1974-03-21 1975-07-29 Central Soya Co Purification of ethanol extractant in soy protein concentrate process
US4454804A (en) 1981-06-18 1984-06-19 Carnation Company Apparatus for incorporating additives in extruded foods
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US5231020A (en) 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
AU1871492A (en) 1991-03-29 1992-11-02 Board Of Trustees Of The University Of Illinois, The Production fo transgenic soybean plants
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5631152A (en) 1994-10-26 1997-05-20 Monsanto Company Rapid and efficient regeneration of transgenic plants
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
CA2266423A1 (en) 1996-09-27 1998-04-02 Maxygen, Inc. Methods for optimization of gene therapy by recursive sequence shuffling and selection
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
EP1717322B1 (en) 1997-01-17 2012-07-18 Codexis Mayflower Holdings, LLC Evolution of whole cells and organisms by recursive sequence recombination
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
GB9703146D0 (en) 1997-02-14 1997-04-02 Innes John Centre Innov Ltd Methods and means for gene silencing in transgenic plants
KR20000076363A (ko) 1997-03-18 2000-12-26 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 Dna 라이브러리를 제조하기 위한 시험관내 방법
DE69840382D1 (de) 1997-03-18 2009-02-05 Novozymes As Methode zur herstellung einer bibliothek mittels dna-shuffling
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6153410A (en) 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
CN1266460A (zh) 1997-06-05 2000-09-13 卡尔金有限责任公司 二酰甘油酰基转移酶蛋白
AU1124499A (en) 1997-10-28 1999-05-17 Maxygen, Inc. Human papillomavirus vectors
AU1449499A (en) 1997-10-31 1999-05-24 Maxygen, Incorporated Modification of virus tropism and host range by viral genome shuffling
IL136574A0 (en) 1997-12-08 2001-06-14 California Inst Of Techn A method for forming a polynucleotide of desired properties
EP1053343A2 (en) 1998-02-11 2000-11-22 Maxygen, Inc. Targeting of genetic vaccine vectors
CA2320958A1 (en) 1998-02-11 1999-08-19 Maxygen, Inc. Antigen library immunization
DK1068311T3 (da) 1998-04-08 2011-08-08 Commw Scient Ind Res Org Fremgangsmåder og midler til opnåelse af modificerede fænotyper
US6344548B1 (en) * 1998-06-24 2002-02-05 The Regents Of The University Of California Diacylglycerol o-acyltransferase
JP4700805B2 (ja) 1998-06-29 2011-06-15 ブリストル−マイヤーズ スクウィブ カンパニー 多様性の高いライブラリーを製造する方法
US7135617B2 (en) 1998-07-02 2006-11-14 Calgene Llc Diacylglycerol acyl transferase proteins
BR9911800A (pt) 1998-07-02 2001-02-28 Calgene Llc Proteìnas de diacil glicerol acil transferase
FR2782323B1 (fr) 1998-08-12 2002-01-11 Proteus Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
AU1199000A (en) 1998-09-29 2000-04-17 Maxygen, Inc. Shuffling of codon altered genes
US6100077A (en) 1998-10-01 2000-08-08 The Trustees Of Columbia University In The City Of New York Isolation of a gene encoding diacylglycerol acyltransferase
US6642426B1 (en) 1998-10-05 2003-11-04 David L. Johnson Fluid-bed aromatics alkylation with staged injection of alkylating agents
WO2000032756A2 (en) 1998-12-02 2000-06-08 E.I. Du Pont De Nemours And Company Sequenzes of a putative plant diacylglycerol acyltransferases
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
AU2415200A (en) 1999-01-18 2000-08-01 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
JP2002534965A (ja) 1999-01-19 2002-10-22 マキシジェン, インコーポレイテッド 所望の特徴を有する文字ストリング、ポリヌクレオチドおよびポリペプチドを作製するための方法
AU2001241939A1 (en) 2000-02-28 2001-09-12 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
CA2408326A1 (en) 2000-06-23 2002-01-03 E.I. Dupont De Nemours And Company Recombinant constructs and their use in reducing gene expression
US7705202B2 (en) * 2002-03-16 2010-04-27 The University Of York Transgenic plants expressing enzymes involved in fatty acid biosynthesis
CA2492205A1 (en) 2002-07-31 2004-02-05 Monsanto Technology, Llc Diacylglycerol acyltransferase nucleic acid sequences and associated products
US20040172682A1 (en) 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
US7129089B2 (en) 2003-02-12 2006-10-31 E. I. Du Pont De Nemours And Company Annexin and P34 promoters and use in expression of transgenic genes in plants
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
WO2005063988A1 (en) * 2003-12-23 2005-07-14 Pioneer Hi-Bred International, Inc. Alteration of oil traits in plants
US8685679B2 (en) 2004-11-04 2014-04-01 E I Du Pont De Nemours And Company Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms
WO2006127655A2 (en) 2005-05-20 2006-11-30 Washington State University Enhancement of hydroxy fatty acid accumulation in oilseed plants
BRPI0708492A2 (pt) 2006-03-01 2011-05-31 Pioneer Hi Bred Int composições relacionadas ao lócus 6 de traço quantitativo (qtl6) em milho e método de uso
WO2008006207A2 (en) 2006-07-13 2008-01-17 National Research Council Of Canada Acyl-coa-dependent diacylglycerol acyltransferase 1 (dgat1 ) gene from tropaeolum majus, protein encoded thereby and uses thereof
EP2297310A2 (en) * 2008-05-23 2011-03-23 E. I. du Pont de Nemours and Company Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
AU2009248932B2 (en) 2008-05-23 2015-08-13 E. I. Du Pont De Nemours And Company DGAT genes from yarrowia lipolytica combined with plastidic phosphoglucomutase down regulation for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
US20090291479A1 (en) 2008-05-23 2009-11-26 E. I. Du Pont De Nemours And Company Manipulation of acyl-coa binding protein expression for altered lipid production in microbial hosts
EP2620502A3 (en) 2008-05-23 2013-09-25 E. I. du Pont de Nemours and Company DGAT genes from oleaginous organisms for increased seed storage lipid production and altered fatty acid profiles in oilseed plants

Also Published As

Publication number Publication date
BRPI0909611B1 (pt) 2018-09-11
CA2723427A1 (en) 2009-11-26
AU2015213370A1 (en) 2015-09-03
US10017777B2 (en) 2018-07-10
CA2990650C (en) 2021-02-09
US20160348124A1 (en) 2016-12-01
EP2297310A2 (en) 2011-03-23
BRPI0909611B8 (pt) 2022-12-06
US8455714B2 (en) 2013-06-04
US20180291391A1 (en) 2018-10-11
WO2009143397A3 (en) 2010-04-22
WO2009143397A2 (en) 2009-11-26
US20090293152A1 (en) 2009-11-26
CA2990650A1 (en) 2009-11-26
US20130225799A1 (en) 2013-08-29
US8101819B2 (en) 2012-01-24
AU2009248931A1 (en) 2009-11-26
EP3115459A2 (en) 2017-01-11
US10633666B2 (en) 2020-04-28
CA2723427C (en) 2018-01-23
US20120156360A1 (en) 2012-06-21
US9447386B2 (en) 2016-09-20
EP3115459A3 (en) 2017-04-26

Similar Documents

Publication Publication Date Title
HRP20181833T1 (hr) Ekspresija proteina iz više nukleinskih kiselina
BRPI0909212A2 (pt) Composições e método para sequeciamento de ácido nucléico
GB0922209D0 (en) Proteins, nucleic acid molecules and compositions
BRPI0819526A2 (pt) "fragmento de ácido nucléico isolado, construção recombinante, célula de planta e método para reduzir a expressão de uma sequência alvo"
WO2010028347A3 (en) Continuous directed evolution of proteins and nucleic acids
BRPI0819495A2 (pt) "fragmento de ácido nucleico isolado, construção recombinante, célula de planta e método para reduzir a expressão de uma sequência alvo"
GB2470672B (en) Methods of RNA amplification in the presence of DNA
BRPI0812037A2 (pt) Variantes de uma alfa-amilase com níveis de produção aperfeiçoados em processos de fermentação
EP3699291C0 (en) METHODS FOR ANALYZING UNIQUE NUCLEIC ACID SEQUENCE AND COMPOSITIONS
BRPI0915142A2 (pt) polipeptídeos, ácido nucleico e usos dos mesmos
EP2267146A4 (en) METHOD OF DEATURING PROTEIN WITH ENZYMES
EP4212630C0 (en) HEIGHT-BASED GENOMIC ANALYSIS FOR PRENATAL DIAGNOSIS
EP2414547A4 (en) MULTIPLE PRIMER REINFORCEMENT PROCESS FOR BARODING TARGET NUCLEIC ACIDS
BRPI1006830A2 (pt) composições de leveduras entrelaçadas com argila e método de utilização das mesmas
WO2009092035A3 (en) Methods and compositions for the analysis of biological molecules
DK1984502T3 (da) Nukleinsyre
WO2009114475A3 (en) Compositions comprising human pcsk9 and apolipoprotein b sirna and methods of use
WO2012027572A3 (en) Methods for nucleic acid capture and sequencing
EP2297182A4 (en) HIGHLY CHARGED PROTEINS USED FOR CELL PENETRATION
EP2315619A4 (en) PROCESS FOR EXTRACTING NUCLEIC ACIDS
WO2008067423A3 (en) Methods of improving the introduction of dna into bacterial cells
WO2010039802A3 (en) Methods and compositions for isolating nucleic acid
ITBO20080047A1 (it) Azionamento elettrico e metodo di pilotaggio dello stesso.
BRPI0817199A2 (pt) Identificaçãob e isolamento de células fetais e ácido nucleico
FI20085039A0 (fi) Nukleiinihappokoettimia ja laaja-alaisia alukkeita

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 22/05/2009, OBSERVADAS AS CONDICOES LEGAIS.

B25A Requested transfer of rights approved

Owner name: PIONEER HI-BRED INTERNATIONAL, INC. (US) ; CORTEVA AGRISCIENCE LLC (US)