BRPI0909611B8 - Método de aumento do teor total de ácido graxo de uma célula de oleaginosa, ácido nucleico recombinante, construção de dna recombinante e método de produção de uma semente oleaginosa - Google Patents
Método de aumento do teor total de ácido graxo de uma célula de oleaginosa, ácido nucleico recombinante, construção de dna recombinante e método de produção de uma semente oleaginosaInfo
- Publication number
- BRPI0909611B8 BRPI0909611B8 BRPI0909611A BRPI0909611A BRPI0909611B8 BR PI0909611 B8 BRPI0909611 B8 BR PI0909611B8 BR PI0909611 A BRPI0909611 A BR PI0909611A BR PI0909611 A BRPI0909611 A BR PI0909611A BR PI0909611 B8 BRPI0909611 B8 BR PI0909611B8
- Authority
- BR
- Brazil
- Prior art keywords
- fatty acid
- oilseed
- total fatty
- acid content
- recombinant
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Food Science & Technology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Agronomy & Crop Science (AREA)
- Fats And Perfumes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
POLINUCLEOTÍDEO RECOMBINANTE, CONSTRUÇÕES DE DNA RECOMBINANTE, MÉTODO DE AUMENTO DO TEOR TOTAL DE ÁCIDO GRAXO DE UMA SEMENTE, MÉTODO DE AUMENTO DO TEOR TOTAL DE ÁCIDO GRAXO DE UMA CÉLULA DE OLEAGINOSA, ÁCIDOS NUCLEICOS RECOMBINANTES, MÉTODO DE PRODUÇÃO DE UMA SEMENTE OLEAGINOSA, MÉTODOS PARA PRODUZIR UM PRODUTO A PARTIR DA SEMENTE OLEAGINOSA E PRODUTO OU SUBPRODUTO. A presente invenção descreve oleaginosas transgênicas que possuem teor total de ácido graxo aumentado em pelo menos 10% e perfis de ácidos graxos alterados em comparação com o teor total de ácido graxo de oleaginosas segregante nulas. Genes DGAT inovadores são utilizados para atingir o aumento dos lipídios de armazenagem de sementes.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US5557908P | 2008-05-23 | 2008-05-23 | |
| PCT/US2009/044926 WO2009143397A2 (en) | 2008-05-23 | 2009-05-22 | Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BRPI0909611A2 BRPI0909611A2 (pt) | 2017-05-30 |
| BRPI0909611B1 BRPI0909611B1 (pt) | 2018-09-11 |
| BRPI0909611B8 true BRPI0909611B8 (pt) | 2022-12-06 |
Family
ID=41110732
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0909611A BRPI0909611B8 (pt) | 2008-05-23 | 2009-05-22 | Método de aumento do teor total de ácido graxo de uma célula de oleaginosa, ácido nucleico recombinante, construção de dna recombinante e método de produção de uma semente oleaginosa |
Country Status (6)
| Country | Link |
|---|---|
| US (5) | US8101819B2 (pt) |
| EP (2) | EP2297310A2 (pt) |
| AU (2) | AU2009248931A1 (pt) |
| BR (1) | BRPI0909611B8 (pt) |
| CA (2) | CA2723427C (pt) |
| WO (1) | WO2009143397A2 (pt) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105123532B (zh) | 2006-07-14 | 2018-12-18 | 联邦科学技术研究组织 | 改变水稻的脂肪酸组成 |
| DE102006054481A1 (de) * | 2006-11-18 | 2008-05-21 | Eppendorf Ag | Vakuumkonzentrator und Verfahren zur Vakuumkonzentration |
| AU2009221599A1 (en) * | 2008-03-07 | 2009-09-11 | Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food | Diacyglycerol acyltransferases from flax |
| BRPI0908642A2 (pt) | 2008-05-23 | 2020-06-23 | E.I. Du Pont De Mours And Company | Polinucleotídeo recombinante, construção de dna recombinante, método para aumentar o teor total de ácido graxo de uma semente, produto ou subproduto, método para produzir uma semente de oleaginosa com um teor total de ácidos graxos aumentado e método para produzir um produto a partir de uma semente de oleaginosa |
| CA2723427C (en) * | 2008-05-23 | 2018-01-23 | E. I. Du Pont De Nemours And Company | Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants |
| US8143476B2 (en) | 2008-05-23 | 2012-03-27 | E I Du Pont De Nemours And Company | DGAT genes from Yarrowia lipolytica combined with plastidic phosphoglucomutase down regulation for increased seed storage lipid production and altered fatty acid profiles in oilseed plants |
| CN102202498B (zh) | 2008-07-21 | 2016-09-07 | 澳大利亚联邦科学与工业研究组织 | 改良的棉籽油及应用 |
| CA2768737A1 (en) | 2008-07-21 | 2010-01-28 | Commonwealth Scientific And Industrial Research Organisation | Improved vegetable oils and uses therefor |
| ES2640100T3 (es) | 2010-06-28 | 2017-10-31 | Commonwealth Scientific And Industrial Research Organisation | Métodos para producir lípidos |
| US9410132B2 (en) * | 2010-11-04 | 2016-08-09 | Ben-Gurion University Of The Negev Research And Development Authority | Acyl-CoA: diacylglycerol acyltransferase 1-like gene (PtDGAT1) and uses thereof |
| US10036030B2 (en) | 2011-12-22 | 2018-07-31 | E I Du Pont De Nemours And Company | Use of the soybean sucrose synthase promoter to increase plant seed lipid content |
| US8809026B2 (en) | 2011-12-27 | 2014-08-19 | Commonwealth Scientific And Industrial Research Organisation | Processes for producing lipids |
| US11639507B2 (en) | 2011-12-27 | 2023-05-02 | Commonwealth Scientific And Industrial Research Organisation | Processes for producing lipids |
| CA3203397A1 (en) | 2011-12-27 | 2013-07-04 | Nuseed Global Innovation Ltd | Processes for producing lipids |
| EP2807258B1 (en) | 2012-01-23 | 2017-04-26 | E. I. du Pont de Nemours and Company | Down-regulation of gene expression using artificial micrornas for silencing fatty acid biosynthestic genes |
| CA2871503C (en) | 2012-04-25 | 2023-08-29 | Commonwealth Scientific And Industrial Research Organisation | High oleic acid oils |
| WO2014049580A2 (en) * | 2012-09-28 | 2014-04-03 | Jawaharlal Nehru Centre For Advanced Scientific Research | Assay to monitor autophagy, a method and kit thereof |
| US11312971B2 (en) | 2012-10-30 | 2022-04-26 | Agresearch Limited | Enhanced acyltransferase polynucleotides, polypeptides and methods of use |
| WO2014068439A2 (en) | 2012-10-30 | 2014-05-08 | Agresearch Limited | Improved acyltransferase polynucleotides, polypeptides, and methods of use |
| MX359167B (es) * | 2012-10-30 | 2018-09-18 | Agresearch Ltd | Polinucleotidos, polipeptidos y metodos de uso novedosos de aciltransferasa. |
| AU2013365731B2 (en) | 2012-12-21 | 2018-11-22 | The New Zealand Institute For Plant And Food Research Limited | Regulation of gene expression |
| US20140289908A1 (en) * | 2013-03-25 | 2014-09-25 | The Governors Of The University Of Alberta | Gene combinations for producing punicic acid in transgenic plants |
| BR112016001918A2 (pt) * | 2013-07-31 | 2017-08-29 | Du Pont | Semente de soja transgênica, farelo, progênie, produtos e subprodutos, métodos para aumentar ácidos graxos totais e a percentagem de ácidos graxos totais, terceira planta transgênica e alimento ou ração |
| AR100159A1 (es) | 2014-04-22 | 2016-09-14 | Du Pont | Genes de anhidrasa carbónica plastídica para el incremento de aceite en semillas con expresión incrementada de dgat |
| WO2016004473A1 (en) | 2014-07-07 | 2016-01-14 | Commonwealth Scientific And Industrial Research Organisation | Processes for producing industrial products from plant lipids |
| US11859193B2 (en) | 2016-09-02 | 2024-01-02 | Nuseed Global Innovation Ltd. | Plants with modified traits |
| BR112019018200B1 (pt) | 2017-03-03 | 2024-02-20 | Pioneer Hi-Bred International, Inc | Método para medir a quantidade de um sucrosil-oligossacarídeo,método para medir estaquiose, método para processar sementes de soja geneticamente modificadas |
| CA3099278A1 (en) | 2018-06-01 | 2019-12-05 | Pioneer Hi-Bred International, Inc. | Modified seed oil content by gene editing |
| US20240228985A1 (en) * | 2021-05-17 | 2024-07-11 | Pioneer Hi-Bred International, Inc | Modified seed oil content in plants |
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| BRPI0908642A2 (pt) | 2008-05-23 | 2020-06-23 | E.I. Du Pont De Mours And Company | Polinucleotídeo recombinante, construção de dna recombinante, método para aumentar o teor total de ácido graxo de uma semente, produto ou subproduto, método para produzir uma semente de oleaginosa com um teor total de ácidos graxos aumentado e método para produzir um produto a partir de uma semente de oleaginosa |
| CA2723427C (en) * | 2008-05-23 | 2018-01-23 | E. I. Du Pont De Nemours And Company | Novel dgat genes for increased seed storage lipid production and altered fatty acid profiles in oilseed plants |
| US20090291479A1 (en) | 2008-05-23 | 2009-11-26 | E. I. Du Pont De Nemours And Company | Manipulation of acyl-coa binding protein expression for altered lipid production in microbial hosts |
| US8143476B2 (en) | 2008-05-23 | 2012-03-27 | E I Du Pont De Nemours And Company | DGAT genes from Yarrowia lipolytica combined with plastidic phosphoglucomutase down regulation for increased seed storage lipid production and altered fatty acid profiles in oilseed plants |
-
2009
- 2009-05-22 CA CA2723427A patent/CA2723427C/en active Active
- 2009-05-22 EP EP09751625A patent/EP2297310A2/en not_active Withdrawn
- 2009-05-22 EP EP16180899.3A patent/EP3115459A3/en not_active Withdrawn
- 2009-05-22 CA CA2990650A patent/CA2990650C/en active Active
- 2009-05-22 BR BRPI0909611A patent/BRPI0909611B8/pt active IP Right Grant
- 2009-05-22 WO PCT/US2009/044926 patent/WO2009143397A2/en not_active Ceased
- 2009-05-22 AU AU2009248931A patent/AU2009248931A1/en not_active Abandoned
- 2009-05-22 US US12/470,569 patent/US8101819B2/en active Active
-
2011
- 2011-12-19 US US13/329,939 patent/US8455714B2/en active Active
-
2013
- 2013-05-07 US US13/888,882 patent/US9447386B2/en active Active
-
2015
- 2015-08-13 AU AU2015213370A patent/AU2015213370A1/en not_active Abandoned
-
2016
- 2016-08-17 US US15/239,069 patent/US10017777B2/en active Active
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2018
- 2018-06-11 US US16/005,018 patent/US10633666B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| AU2009248931A1 (en) | 2009-11-26 |
| BRPI0909611B1 (pt) | 2018-09-11 |
| US10017777B2 (en) | 2018-07-10 |
| EP3115459A2 (en) | 2017-01-11 |
| WO2009143397A2 (en) | 2009-11-26 |
| US20120156360A1 (en) | 2012-06-21 |
| US20180291391A1 (en) | 2018-10-11 |
| US8101819B2 (en) | 2012-01-24 |
| US8455714B2 (en) | 2013-06-04 |
| EP3115459A3 (en) | 2017-04-26 |
| CA2990650C (en) | 2021-02-09 |
| US20130225799A1 (en) | 2013-08-29 |
| CA2723427C (en) | 2018-01-23 |
| WO2009143397A3 (en) | 2010-04-22 |
| BRPI0909611A2 (pt) | 2017-05-30 |
| US9447386B2 (en) | 2016-09-20 |
| US10633666B2 (en) | 2020-04-28 |
| US20160348124A1 (en) | 2016-12-01 |
| EP2297310A2 (en) | 2011-03-23 |
| US20090293152A1 (en) | 2009-11-26 |
| CA2723427A1 (en) | 2009-11-26 |
| CA2990650A1 (en) | 2009-11-26 |
| AU2015213370A1 (en) | 2015-09-03 |
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