BRPI0913107A2 - Reagente de lise, ligação e/ou lavagem, uso do mesmo, método e kit para isolamento e/ou purificação de ácidos nucleicos de uma amostra biológica contendo ácidos nucleicos, e, uso de tensoativos não-iônicos com base em polixietileno. - Google Patents
Reagente de lise, ligação e/ou lavagem, uso do mesmo, método e kit para isolamento e/ou purificação de ácidos nucleicos de uma amostra biológica contendo ácidos nucleicos, e, uso de tensoativos não-iônicos com base em polixietileno.Info
- Publication number
- BRPI0913107A2 BRPI0913107A2 BRPI0913107-8A BRPI0913107A BRPI0913107A2 BR PI0913107 A2 BRPI0913107 A2 BR PI0913107A2 BR PI0913107 A BRPI0913107 A BR PI0913107A BR PI0913107 A2 BRPI0913107 A2 BR PI0913107A2
- Authority
- BR
- Brazil
- Prior art keywords
- nucleic acids
- polyxyethylene
- lysis
- kit
- isolation
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C43/00—Ethers; Compounds having groups, groups or groups
- C07C43/02—Ethers
- C07C43/20—Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring
- C07C43/23—Ethers having an ether-oxygen atom bound to a carbon atom of a six-membered aromatic ring containing hydroxy or O-metal groups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Detergent Compositions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102008026058A DE102008026058A1 (de) | 2008-05-30 | 2008-05-30 | Lyse, Binde- und/oder Waschreagenz verwendbar zur Isolierung und/oder Reinigung von Nukleinsäuren |
| DE102008026058.4 | 2008-05-30 | ||
| PCT/EP2009/056268 WO2009144182A1 (de) | 2008-05-30 | 2009-05-25 | Lyse-, binde- und/oder waschreagenz verwendbar zur isolierung und/oder reinigung von nukleinsäuren |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BRPI0913107A2 true BRPI0913107A2 (pt) | 2015-08-04 |
| BRPI0913107B1 BRPI0913107B1 (pt) | 2018-04-17 |
Family
ID=40941635
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0913107-8A BRPI0913107B1 (pt) | 2008-05-30 | 2009-05-25 | Reagente de lise, ligação e/ou lavagem, uso do mesmo, método e kit para isolamento e/ou purificação de ácidos nucleicos de uma amostra biológica contendo ácidos nucleicos, e, uso de tensoativos não-iônicos com base em polioxietileno |
Country Status (12)
| Country | Link |
|---|---|
| US (5) | US9617532B2 (pt) |
| EP (2) | EP2285957B1 (pt) |
| JP (5) | JP6059430B2 (pt) |
| KR (6) | KR102470830B1 (pt) |
| CN (2) | CN106119243A (pt) |
| AU (1) | AU2009253197B2 (pt) |
| BR (1) | BRPI0913107B1 (pt) |
| CA (1) | CA2725586C (pt) |
| DE (1) | DE102008026058A1 (pt) |
| ES (1) | ES2534773T3 (pt) |
| MX (1) | MX2010012849A (pt) |
| WO (1) | WO2009144182A1 (pt) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
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| DE102008026058A1 (de) * | 2008-05-30 | 2009-12-03 | Qiagen Gmbh | Lyse, Binde- und/oder Waschreagenz verwendbar zur Isolierung und/oder Reinigung von Nukleinsäuren |
| EP2473596B1 (en) * | 2009-09-03 | 2017-12-13 | Becton, Dickinson and Company | Methods and compositions for direct chemical lysis |
| EP2325312A1 (de) * | 2009-11-19 | 2011-05-25 | Qiagen GmbH | Verfahren zur selektiven Anreicherung und Isolierung mikrobieller und optional zusätzlich viraler Nukleinsäuren |
| CN101717421A (zh) * | 2009-12-15 | 2010-06-02 | 大连水产学院 | 核酸纯化柱再生的方法 |
| WO2011104032A1 (en) | 2010-02-26 | 2011-09-01 | Qiagen Gmbh | Method for isolating rna from a rna and dna containing sample |
| US20110318743A1 (en) * | 2010-06-28 | 2011-12-29 | Life Technologies Corporation | Methods, workflows, kits, apparatuses, and computer program media for nucleic acid sample preparation for nucleic acid sequencing |
| CN103764827B (zh) | 2011-07-04 | 2018-04-24 | 恰根有限公司 | 可用于分离和／或纯化核酸的试剂 |
| WO2013037401A1 (en) * | 2011-09-13 | 2013-03-21 | Qiagen Gmbh | Method for isolating nucleic acids from a veterinary whole blood sample |
| CN113355321A (zh) | 2011-09-26 | 2021-09-07 | 凯杰有限公司 | 用于分离细胞外核酸的快速方法 |
| CN102358899B (zh) * | 2011-09-30 | 2013-03-20 | 陕西师范大学 | 硅质膜离心吸附柱再生液 |
| WO2014128129A1 (en) * | 2013-02-25 | 2014-08-28 | Biocartis N.V. | Isolation of nucleic acids |
| WO2015103320A1 (en) * | 2013-12-31 | 2015-07-09 | Canon U.S. Life Sciences, Inc. | Methods, devices and systems for emulsion/droplet pcr |
| EP3218480A1 (en) * | 2014-11-14 | 2017-09-20 | Corning Incorporated | Methods and kits for post-ivt rna purification |
| WO2016198519A1 (en) * | 2015-06-09 | 2016-12-15 | Biocartis N.V. | Automatable method for nucleic acid isolation |
| JP6787916B2 (ja) * | 2015-06-10 | 2020-11-18 | キアゲン ゲーエムベーハー | アニオン交換粒子を使用する細胞外核酸を単離する方法 |
| CN106000102B (zh) * | 2016-06-02 | 2018-08-31 | 北京师范大学 | 绒毛浆作为吸收体收集和保存尿液中蛋白质的方法及装置 |
| EP3684383A4 (en) * | 2017-09-20 | 2021-07-14 | Molecular Stethoscope, Inc. | METHODS AND SYSTEMS FOR DETECTION OF TISSUE DISORDERS |
| WO2019100620A1 (zh) * | 2017-11-24 | 2019-05-31 | 浙江今复康生物科技有限公司 | 痰液的保存方法和从痰液中快速提取核酸的方法 |
| CN111521780B (zh) * | 2019-02-01 | 2024-03-26 | 科美诊断技术股份有限公司 | 一种丙型肝炎病毒抗原抗体联检的试剂盒及其应用 |
| US20220090166A1 (en) * | 2019-03-04 | 2022-03-24 | Siemens Healthcare Gmbh | Preparation methods and apparatus adapted to filter small nucleic acids from biological samples |
| JP7602467B2 (ja) * | 2019-09-19 | 2024-12-18 | 三洋化成工業株式会社 | 磁性粒子組成物、磁性粒子組成物の核酸分離用途での使用、磁性粒子組成物を得るためのキット、磁性粒子、カオトロピック塩及び分離精製方法 |
| TWI733302B (zh) * | 2020-01-09 | 2021-07-11 | 創想生物科技有限公司 | 核酸分離方法及其系統 |
| GB202001034D0 (en) * | 2020-01-24 | 2020-03-11 | Ge Healthcare Uk Ltd | Method and kit for DNA isolation |
| CN112226490A (zh) * | 2020-11-12 | 2021-01-15 | 苏州创澜生物科技有限公司 | 一种用于dna提取的裂解缓冲液 |
| CN112176033A (zh) * | 2020-11-12 | 2021-01-05 | 苏州创澜生物科技有限公司 | 一种用于rna提取的裂解缓冲液 |
| CN112239775A (zh) * | 2020-11-12 | 2021-01-19 | 苏州创澜生物科技有限公司 | 一种用于核酸提取的洗涤缓冲液 |
| US12390808B2 (en) | 2021-01-12 | 2025-08-19 | Definitive Biotechnologies Llc | Device and method for detecting nucleic acids in biological samples |
| CN113355320A (zh) * | 2021-01-25 | 2021-09-07 | 汉远化生医国际科技(北京)有限公司 | 用于分离和/或纯化核酸的裂解、结合、洗涤和/或洗脱试剂 |
| CN113186037B (zh) * | 2021-04-23 | 2022-12-13 | 山东博弘基因科技有限公司 | 一种核酸清除剂 |
| CN113462682A (zh) * | 2021-06-18 | 2021-10-01 | 汉远化生医国际科技(北京)有限公司 | 用于分离和/或纯化生物rna的裂解、结合、洗涤和/或洗脱试剂 |
| CN113717969A (zh) * | 2021-08-11 | 2021-11-30 | 汉远化生医国际科技(北京)有限公司 | 一类植物组织核酸提取试剂和方法 |
| CN115851701A (zh) * | 2022-11-30 | 2023-03-28 | 成都罗宁生物科技有限公司 | 一种提取微生物dna的试剂盒及其使用方法 |
| CN116949130A (zh) * | 2023-01-04 | 2023-10-27 | 深圳市真迈生物科技有限公司 | 一种试剂盒及一种核酸提取方法 |
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| JP3018802B2 (ja) | 1992-12-04 | 2000-03-13 | 和光純薬工業株式会社 | 全血液検体からのｄｎａ抽出方法及び抽出キット |
| DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
| JP4113580B2 (ja) * | 1994-02-07 | 2008-07-09 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | エンドトキシンを減量又は除去する方法 |
| US6180778B1 (en) | 1994-02-11 | 2001-01-30 | Qiagen Gmbh | Process for the separation of double-stranded/single-stranded nucleic acid structures |
| KR19990022612A (ko) * | 1995-06-08 | 1999-03-25 | 퍼니스 챨스 엠 | 디엔에이 추출방법 및 추출장치 |
| ATE284891T1 (de) | 1996-02-14 | 2005-01-15 | Biomerieux Bv | Isolierung von einzelsträngigen nukleinsäuren |
| ES2286852T3 (es) * | 1997-08-04 | 2007-12-01 | Advanced Life Science Institute, Inc. | Metodos para detectar o analizar virus. |
| US5932422A (en) | 1997-11-14 | 1999-08-03 | Millennium Pharmaceuticals, Inc. | Modulation of drug resistance via ubiquitin carboxy-terminal hydrolase |
| US20050053962A1 (en) | 1998-01-27 | 2005-03-10 | Gary Blackburn | Amplification of nucleic acids with electronic detection |
| US6599713B1 (en) | 1999-03-29 | 2003-07-29 | Asahi Kasei Kabushiki Kaisha | Method for quantitating leukocyte count in whole blood sample |
| WO2000077235A1 (en) * | 1999-06-16 | 2000-12-21 | University Of Cincinnati | Agent and process for isolation of extra-chromosomal nucleic acids |
| DE10006662A1 (de) * | 2000-02-15 | 2001-08-23 | Antigen Produktions Gmbh | Gefäß zur Nukleinsäureanalytik |
| AU6380001A (en) | 2000-03-24 | 2001-10-03 | Qiagen Gmbh | Porous ferro- or ferrimagnetic glass particles for isolating molecules |
| DE10147439B4 (de) | 2001-09-26 | 2014-01-30 | Qiagen Gmbh | Verfahren zur Isolierung von DNA aus biologischen Proben |
| US20030180936A1 (en) * | 2002-03-15 | 2003-09-25 | Memarzadeh Bahram Eric | Method for the purification, production and formulation of oncolytic adenoviruses |
| JP4103468B2 (ja) * | 2002-06-28 | 2008-06-18 | 日本電気株式会社 | 差動回路と増幅回路及び該増幅回路を用いた表示装置 |
| DE10231659B4 (de) * | 2002-07-12 | 2006-01-19 | Preanalytix Gmbh | Zusammensetzung zum Binden von Nukleinsäure an eine Festphase |
| US20040025916A1 (en) * | 2002-08-12 | 2004-02-12 | Shih-Shin Kuo | Rib structure of a four-folded umbrella |
| EP1656443A4 (en) * | 2003-05-02 | 2007-06-06 | Sigma Aldrich Co | SOLID PHASE CELLYSIS AND IMPACT PLATFORM |
| US20050142570A1 (en) * | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent |
| AU2005200670B2 (en) * | 2004-02-20 | 2007-05-03 | F. Hoffmann-La Roche Ag | Adsorption of nucleic acids to a solid phase |
| JP2006238854A (ja) * | 2004-03-26 | 2006-09-14 | Fuji Photo Film Co Ltd | 核酸の分離精製方法 |
| AU2005277527A1 (en) * | 2004-08-18 | 2006-03-02 | Preanalytix Gmbh | Additive, method, and article for DNA collection, stabilization, and purification |
| US7727718B2 (en) * | 2005-01-04 | 2010-06-01 | Molecular Research Center, Inc. | Reagents for storage and preparation of samples for DNA analysis |
| DE102005057334A1 (de) | 2005-11-28 | 2007-06-06 | Aj Innuscreen Gmbh | Verfahren zur Isolierung von Nukleinsäuren aus beliebigen Ausgangsmaterialien |
| CN101017140A (zh) * | 2006-02-08 | 2007-08-15 | 河南省生物工程技术研究中心 | 人肠道病毒(ev)荧光定量pcr检测技术 |
| JP2007244375A (ja) * | 2006-02-14 | 2007-09-27 | Toyobo Co Ltd | リボ核酸の分離精製方法 |
| EP1932913B1 (en) | 2006-12-11 | 2013-01-16 | Roche Diagnostics GmbH | Nucleic acid isolation using polidocanol and derivatives |
| US20090048439A1 (en) * | 2007-08-06 | 2009-02-19 | Weisburg William G | Isolation of nucleic acids molecules using modified solid supports |
| KR101053023B1 (ko) | 2008-02-14 | 2011-08-01 | (주)바이오니아 | 구형 실리카 자성입자 및 이의 제조방법 |
| DE102008026058A1 (de) | 2008-05-30 | 2009-12-03 | Qiagen Gmbh | Lyse, Binde- und/oder Waschreagenz verwendbar zur Isolierung und/oder Reinigung von Nukleinsäuren |
| WO2013037401A1 (en) * | 2011-09-13 | 2013-03-21 | Qiagen Gmbh | Method for isolating nucleic acids from a veterinary whole blood sample |
| CN103142458B (zh) | 2013-01-22 | 2015-09-09 | 莱普德制药有限公司 | 无成瘾性镇痛缓释递药系统的组方与制备方法 |
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2008
- 2008-05-30 DE DE102008026058A patent/DE102008026058A1/de not_active Ceased
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2009
- 2009-05-25 KR KR1020217030942A patent/KR102470830B1/ko active Active
- 2009-05-25 KR KR1020107026724A patent/KR20110022570A/ko not_active Ceased
- 2009-05-25 BR BRPI0913107-8A patent/BRPI0913107B1/pt active IP Right Grant
- 2009-05-25 KR KR1020217030943A patent/KR102586355B1/ko active Active
- 2009-05-25 CA CA2725586A patent/CA2725586C/en active Active
- 2009-05-25 WO PCT/EP2009/056268 patent/WO2009144182A1/de not_active Ceased
- 2009-05-25 EP EP09753837.5A patent/EP2285957B1/de active Active
- 2009-05-25 KR KR1020237012905A patent/KR20230058529A/ko not_active Ceased
- 2009-05-25 EP EP15152388.3A patent/EP2899276B1/de active Active
- 2009-05-25 AU AU2009253197A patent/AU2009253197B2/en active Active
- 2009-05-25 KR KR1020177011253A patent/KR102207033B1/ko active Active
- 2009-05-25 US US12/994,308 patent/US9617532B2/en active Active
- 2009-05-25 ES ES09753837.5T patent/ES2534773T3/es active Active
- 2009-05-25 KR KR1020197022409A patent/KR20190092617A/ko not_active Ceased
- 2009-05-25 JP JP2011510962A patent/JP6059430B2/ja active Active
- 2009-05-25 MX MX2010012849A patent/MX2010012849A/es active IP Right Grant
- 2009-05-25 CN CN201610478449.8A patent/CN106119243A/zh active Pending
- 2009-05-25 CN CN200980120902.6A patent/CN102046793B/zh active Active
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2015
- 2015-05-28 JP JP2015108530A patent/JP6499015B2/ja active Active
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2016
- 2016-09-29 JP JP2016191445A patent/JP6560177B2/ja active Active
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2017
- 2017-02-23 US US15/440,699 patent/US20170159042A1/en not_active Abandoned
- 2017-02-23 US US15/440,682 patent/US20170159041A1/en not_active Abandoned
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2019
- 2019-01-25 JP JP2019011480A patent/JP7069059B2/ja active Active
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2021
- 2021-04-20 US US17/235,403 patent/US12139703B2/en active Active
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2022
- 2022-02-01 JP JP2022014191A patent/JP2022060265A/ja not_active Withdrawn
- 2022-03-15 US US17/695,270 patent/US20220204968A1/en active Pending
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