BRPI0918882A2 - métodos, sistemas e composições para tolerância aumentada de microorganismo para produção de ácido 3-hidroxipropiônico (3-hp) - Google Patents

métodos, sistemas e composições para tolerância aumentada de microorganismo para produção de ácido 3-hidroxipropiônico (3-hp)

Info

Publication number
BRPI0918882A2
BRPI0918882A2 BRPI0918882A BRPI0918882A BRPI0918882A2 BR PI0918882 A2 BRPI0918882 A2 BR PI0918882A2 BR PI0918882 A BRPI0918882 A BR PI0918882A BR PI0918882 A BRPI0918882 A BR PI0918882A BR PI0918882 A2 BRPI0918882 A2 BR PI0918882A2
Authority
BR
Brazil
Prior art keywords
compositions
methods
hydroxypropionic acid
systems
production
Prior art date
Application number
BRPI0918882A
Other languages
English (en)
Inventor
d lynch Michael
T Gill Ryan
E Warnecke-Lipscomb Tanya
Original Assignee
Opx Biotechnologies Inc
Univ Colorado Regents
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Opx Biotechnologies Inc, Univ Colorado Regents filed Critical Opx Biotechnologies Inc
Publication of BRPI0918882A2 publication Critical patent/BRPI0918882A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

métodos, sistemas e composições para tolerância aumentada de microorganismo para produção de ácido 3-hidroxipropiônico (3-hp). a presente invenção se refere a métodos, 5 sistema e composições, incluindo um microorganismo geneticamente modificado, adaptado para exibir tolerância aumentadas a ácido 3-hidroxipropiônico (3-hp), particularmente através de alterações inter-relacionadas a caminhos metabólicos identificados como via do complexo toleragênico do 3-hp.
BRPI0918882A 2008-07-23 2009-07-23 métodos, sistemas e composições para tolerância aumentada de microorganismo para produção de ácido 3-hidroxipropiônico (3-hp) BRPI0918882A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US13586208P 2008-07-23 2008-07-23
US13586108P 2008-07-23 2008-07-23
US8833108P 2008-08-12 2008-08-12
US9693708P 2008-09-15 2008-09-15
PCT/US2009/051607 WO2010011874A2 (en) 2008-07-23 2009-07-23 Methods, systems and compositions for increased microorganism tolerance to and production of 3-hydroxypropionic acid (3-hp)

Publications (1)

Publication Number Publication Date
BRPI0918882A2 true BRPI0918882A2 (pt) 2019-02-26

Family

ID=41570872

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0918882A BRPI0918882A2 (pt) 2008-07-23 2009-07-23 métodos, sistemas e composições para tolerância aumentada de microorganismo para produção de ácido 3-hidroxipropiônico (3-hp)

Country Status (8)

Country Link
US (2) US20110244575A1 (pt)
EP (1) EP2318514A4 (pt)
CN (1) CN102317436B (pt)
AU (1) AU2009273953B2 (pt)
BR (1) BRPI0918882A2 (pt)
CA (1) CA2731509A1 (pt)
MX (1) MX2011000793A (pt)
WO (1) WO2010011874A2 (pt)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2675026A1 (en) * 2007-01-12 2008-07-24 The Regents Of The University Of Colorado, A Body Corporate Compositions and methods for enhancing tolerance for the production of organic chemicals produced by microorganisms
US8048624B1 (en) 2007-12-04 2011-11-01 Opx Biotechnologies, Inc. Compositions and methods for 3-hydroxypropionate bio-production from biomass
US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
MX2012003604A (es) * 2009-09-27 2012-09-12 Opx Biotechnologies Inc Metodo para producir acido 3-hidroxipropionico y otros productos.
US8809027B1 (en) 2009-09-27 2014-08-19 Opx Biotechnologies, Inc. Genetically modified organisms for increased microbial production of 3-hydroxypropionic acid involving an oxaloacetate alpha-decarboxylase
US9090919B2 (en) * 2009-10-29 2015-07-28 Korea Research Institute Of Bioscience And Biotechnology Method of producing 3-hydroxypropionic acid from glycerol
EP2529023A4 (en) * 2010-01-27 2015-06-10 Opx Biotechnologies Inc MICRO-ORGANISM MANUFACTURE FROM HIGH-FLOW CHEMICAL PRODUCTS AND CORRESPONDING COMPOSITIONS, METHODS AND SYSTEMS
BR112012027407B1 (pt) 2010-04-26 2020-04-07 Nippon Shokubai Co., Ltd. resina absorvedora de água tipo ácido poliacrílico (sal), material sanitário contendo a mesma, método para produzir e identificar a mesma e método para produzir ácido poliacrílico (sal)
US9090918B2 (en) 2010-11-22 2015-07-28 Novozymes A/A Compositions and methods for 3-hydroxypropionic acid production
CA2868113A1 (en) 2011-03-22 2012-09-27 Opx Biotechnologies, Inc. Microbial production of chemical products and related compositions, methods and systems
KR101860442B1 (ko) 2011-06-27 2018-05-24 삼성전자주식회사 3-하이드록시프로피온산의 생산을 위한 유전자 조작
MX2015001780A (es) 2012-08-10 2015-09-28 Opx Biotechnologies Inc Microorganismos y métodos para la producción de ácidos grasos y productos derivados de ácidos grasos.
US9587256B2 (en) 2012-09-06 2017-03-07 University Of Georgia Research Foundation, Inc. Sequestration of carbon dioxide with hydrogen to useful products
CN105209626B (zh) * 2012-11-30 2019-09-03 诺维信股份有限公司 通过重组酵母生产3-羟基丙酸
US20150119601A1 (en) 2013-03-15 2015-04-30 Opx Biotechnologies, Inc. Monofunctional mcr + 3-hp dehydrogenase
US10442749B2 (en) 2013-03-15 2019-10-15 Cargill, Incorporated Recovery of 3-hydroxypropionic acid
US20150044746A1 (en) * 2013-03-15 2015-02-12 Opx Biotechnologies, Inc. Method of enhanced bioproduction
EP2976141A4 (en) 2013-03-15 2016-10-05 Cargill Inc FLASHING FOR PRODUCTION CLEANING AND RECOVERY
EP3008179A1 (en) * 2013-06-14 2016-04-20 Technical University of Denmark 3hp tolerance
EP3022310B1 (en) 2013-07-19 2019-10-16 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
KR20150051081A (ko) 2013-11-01 2015-05-11 삼성전자주식회사 전사 인자 조작을 통하여 재프로그래밍된 유전자를 발현하는 내산성이 증가된 클루베로마이세스 마르시아누스
CN103898168B (zh) * 2014-01-16 2017-11-03 华东理工大学 一种安全生产1,3‑丙二醇的方法
AU2015324564B2 (en) 2014-06-11 2021-07-01 Duke University Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
CN104726389B (zh) * 2015-04-14 2018-11-13 中国科学院青岛生物能源与过程研究所 一种3-羟基丙酸耐受性提高的大肠杆菌及其构建方法
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
BR112018011503A2 (pt) 2015-12-07 2018-12-04 Zymergen Inc promotores da corynebacterium glutamicum
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
EP3478845A4 (en) 2016-06-30 2019-07-31 Zymergen, Inc. METHOD OF GENERATING A GLUCOSE PERMEASE LIBRARY AND USES THEREOF
US10544390B2 (en) 2016-06-30 2020-01-28 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CN106755056A (zh) * 2016-12-14 2017-05-31 吴银娣 一种酸胁迫抗性提高的毕赤酵母
US11345938B2 (en) 2017-02-02 2022-05-31 Cargill, Incorporated Genetically modified cells that produce C6-C10 fatty acid derivatives
MY193581A (en) 2017-02-21 2022-10-19 Univ Duke Compositions and methods for robust dynamic metabolic control
CN108728469B (zh) * 2017-04-14 2022-03-01 中国科学院微生物研究所 重组大肠杆菌工程菌的构建及其在生产β-丙氨酸中的应用
US20200095621A1 (en) 2017-05-16 2020-03-26 The Regents Of The University Of California Methods and compositions for 3-hydroxypropionate production
US11634736B2 (en) 2018-02-01 2023-04-25 Inv Nylon Chemicals Americas, Llc Methods and materials for the biosynthesis of compounds involved in the tricarboxylic acid cycle and derivatives and compounds related thereto
CN110295188B (zh) * 2018-03-23 2021-06-15 华东理工大学 一种提高大肠杆菌合成的聚（3-羟基丁酸-co-乳酸）中乳酸组分含量的方法
WO2019246488A1 (en) 2018-06-21 2019-12-26 Duke University Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control
CN109810991B (zh) * 2019-03-02 2021-11-12 昆明理工大学 二氢蝶酸合酶基因folP的用途
CN110129247B (zh) * 2019-05-13 2021-02-26 中国科学院天津工业生物技术研究所 赖氨酸生产菌株的构建方法和应用
CN110157654B (zh) * 2019-05-21 2020-07-28 江南大学 一种纳豆芽孢杆菌重组菌及其构建方法与应用
CN110172435B (zh) * 2019-06-06 2020-12-29 江南大学 一种催化合成2,5-二甲基吡嗪的重组菌
CN110499343B (zh) * 2019-09-11 2021-06-04 鲁东大学 一种酶法制备4-羟基苯乙醛的方法
CN110564757A (zh) * 2019-09-27 2019-12-13 华东理工大学 利用乙酸或其盐生产3-羟基丙酸的代谢工程大肠杆菌菌株的构建方法及应用
EP4100038A4 (en) * 2020-02-03 2024-02-28 Premas Biotech Private Limited RECOMBINANT EXPRESSION PLATFORM, CONSTRUCTS AND METHODS FOR EXPRESSING HARD-TO-EXPRESS PROTEINS (DTE-PS)
CN111235169A (zh) * 2020-02-03 2020-06-05 昆明理工大学 一种GTP环化水解酶I基因folE及应用
KR102639658B1 (ko) * 2020-07-31 2024-02-22 주식회사 엘지화학 3-하이드록시프로피온산의 2단계 제조 방법
CN116121160B (zh) * 2022-10-28 2025-03-14 宁夏伊品生物科技股份有限公司 过表达pyrB基因的基因工程菌及其生产L-精氨酸的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6852517B1 (en) * 1999-08-30 2005-02-08 Wisconsin Alumni Research Foundation Production of 3-hydroxypropionic acid in recombinant organisms
EP1224317B1 (en) * 1999-10-25 2006-03-08 Kosan Biosciences, Inc. Production of polyketides
BR0115877A (pt) * 2000-11-20 2003-10-07 Cargill Inc ácido 3-hidroxipropiÈnico e outros compostos orgánicos
WO2004033646A2 (en) * 2002-10-04 2004-04-22 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
US20060270013A1 (en) * 2003-02-18 2006-11-30 Michel Chateau Method for the production of evolved microorganisms which permit the generation or modification of metabolic pathways
WO2005118719A2 (en) * 2003-12-04 2005-12-15 Cargill, Incorporated Production of 3-hydroxypropionic acid using beta-alanine/pyruvate aminotransferase
WO2007012078A1 (en) * 2005-07-18 2007-01-25 Basf Ag Methionine producing recombinant microorganisms
CN101300356A (zh) * 2005-09-09 2008-11-05 基因组股份公司 用于琥珀酸盐之生长关联性生产的方法和生物
US8030045B2 (en) * 2006-08-30 2011-10-04 Cargill, Incorporated Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production
CA2675026A1 (en) * 2007-01-12 2008-07-24 The Regents Of The University Of Colorado, A Body Corporate Compositions and methods for enhancing tolerance for the production of organic chemicals produced by microorganisms
US8673601B2 (en) * 2007-01-22 2014-03-18 Genomatica, Inc. Methods and organisms for growth-coupled production of 3-hydroxypropionic acid

Also Published As

Publication number Publication date
CA2731509A1 (en) 2010-01-28
EP2318514A2 (en) 2011-05-11
AU2009273953A1 (en) 2010-01-28
AU2009273953B2 (en) 2013-08-29
CN102317436B (zh) 2014-06-25
EP2318514A4 (en) 2013-08-21
US20150056684A1 (en) 2015-02-26
WO2010011874A3 (en) 2010-08-05
MX2011000793A (es) 2011-09-06
CN102317436A (zh) 2012-01-11
WO2010011874A2 (en) 2010-01-28
US20110244575A1 (en) 2011-10-06

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