BRPI9912047B1 - Métodos para avaliar células malignas em uma epiderme in vitro, e para avaliar compostos de teste quanto à toxicidade humana. - Google Patents

Métodos para avaliar células malignas em uma epiderme in vitro, e para avaliar compostos de teste quanto à toxicidade humana.

Info

Publication number: BRPI9912047B1
Authority: BR; Brazil
Prior art keywords: epidermis; evaluating; malignant cells; vitro; methods
Prior art date: 1998-07-13

Application number

BRPI9912047-0B1A

Other languages

English (en)

Inventor

Lynn Allen-Hoffmann

Sandra J Schlosser

Michael A Pickart

Original Assignee

Wisconsin Alumni Res Found

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-07-13

Filing date

1999-07-12

Publication date

2014-03-18

1999-07-12 Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found

2001-04-03 Publication of BRPI9912047A publication Critical patent/BRPI9912047A/pt

2014-03-18 Publication of BRPI9912047B1 publication Critical patent/BRPI9912047B1/pt

2021-07-06 Publication of BRPI9912047B8 publication Critical patent/BRPI9912047B8/pt

Links

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0629—Keratinocytes; Whole skin
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
- C12N5/0698—Skin equivalents
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
- C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1323—Adult fibroblasts
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/30—Coculture with; Conditioned medium produced by tumour cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/02—Drug screening
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/04—Screening or testing on artificial tissues
- C12N2503/06—Screening or testing on artificial skin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/04—Immortalised cells
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Landscapes

Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Life Sciences & Earth Sciences (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Genetics & Genomics (AREA)
General Health & Medical Sciences (AREA)
Cell Biology (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Immunology (AREA)
General Engineering & Computer Science (AREA)
Medicinal Chemistry (AREA)
Urology & Nephrology (AREA)
Molecular Biology (AREA)
Hematology (AREA)
Toxicology (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Physics & Mathematics (AREA)
Animal Behavior & Ethology (AREA)
Pharmacology & Pharmacy (AREA)
Tropical Medicine & Parasitology (AREA)
Chemical Kinetics & Catalysis (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Food Science & Technology (AREA)
General Chemical & Material Sciences (AREA)
Analytical Chemistry (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Dermatology (AREA)
Oncology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Investigating Or Analysing Biological Materials (AREA)

BRPI9912047 1998-07-13 1999-07-12 métodos para avaliar células malignas em uma epiderme in vitro, e para avaliar compostos de teste quanto à toxicidade humana. BRPI9912047B8 (pt)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US09/114,557 US5989837A (en)	1998-07-13	1998-07-13	Immortalized human keratinocyte cell line
PCT/US1999/015671 WO2000003244A2 (en)	1998-07-13	1999-07-12	Immortalized human keratinocyte cell line

Publications (3)

Publication Number	Publication Date
BRPI9912047A BRPI9912047A (pt)	2001-04-03
BRPI9912047B1 true BRPI9912047B1 (pt)	2014-03-18
BRPI9912047B8 BRPI9912047B8 (pt)	2021-07-06

Family

ID=22355986

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
BRPI9912047 BRPI9912047B8 (pt)	1998-07-13	1999-07-12	métodos para avaliar células malignas em uma epiderme in vitro, e para avaliar compostos de teste quanto à toxicidade humana.

Country Status (15)

Country	Link
US (6)	US5989837A (pt)
EP (2)	EP1117423B1 (pt)
JP (1)	JP3557172B2 (pt)
KR (1)	KR100461853B1 (pt)
CN (1)	CN1197960C (pt)
AT (2)	ATE550439T1 (pt)
AU (1)	AU758141B2 (pt)
BR (1)	BRPI9912047B8 (pt)
CA (1)	CA2336717C (pt)
DE (1)	DE69931941T2 (pt)
DK (1)	DK1117423T3 (pt)
ES (2)	ES2267277T3 (pt)
IL (1)	IL140608A (pt)
NZ (1)	NZ509184A (pt)
WO (1)	WO2000003244A2 (pt)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6964869B2 (en) *	1998-07-13	2005-11-15	Wisconsin Alumni Research Foundation	Method and composition for skin grafts
US20060030044A1 (en) *	1998-07-13	2006-02-09	Allen-Hoffmann B Lynn	Method and composition for skin grafts
US5989837A (en) *	1998-07-13	1999-11-23	Wisconsin Alumni Research Foundation	Immortalized human keratinocyte cell line
US6974697B2 (en) *	2001-03-02	2005-12-13	Stratech Corporation	Skin substitutes with improved barrier function
EP1404809B1 (en) *	2001-03-02	2013-12-11	Stratatech Corporation	Improved skin substitutes and uses thereof
CA2444699C (en) *	2001-04-24	2012-08-07	Wisconsin Alumni Research Foundation	Method and composition for skin grafts
EP1549738B1 (en)	2002-04-30	2010-04-21	Stratatech Corporation	Keratinocytes expressing exogenous angiogenic growth factors
US20030219416A1 (en) *	2002-05-24	2003-11-27	Marcia Simon	Long-lived keratinocytes
EP1546392A4 (en) *	2002-08-02	2006-05-24	Stratatech Corp	DETECTION OF SPECIFIC DNA FROM SPECIES
US9453251B2 (en)	2002-10-08	2016-09-27	Pfenex Inc.	Expression of mammalian proteins in Pseudomonas fluorescens
FR2847268B1 (fr) *	2002-11-19	2006-09-29	Coletica	Procede d'identification d'une modification eventuelle d'au moins un parametre biologique mettant en oeuvre des cellules vivantes soumises a un stress et des cellules vivantes non soumises a ce meme stress
FR2847269B1 (fr) *	2002-11-19	2006-07-28	Coletica	Procede d'identification d'une modification eventuelle d'au moins un parametre biologique mettant en oeuvre des cellules vivantes jeunes et agees
WO2004053097A2 (en) *	2002-12-10	2004-06-24	Medical College Of Georgia Research Institute, Inc.	Chemopreventive and therapeutic aspects of polyphenolic compositions and assays
EP1622609A4 (en) *	2003-04-29	2008-09-03	Smithkline Beecham Corp	METHODS OF TREATING DEGENERATIVE DISEASES / LESIONS
CA2534349C (en) *	2003-08-01	2012-04-10	Stratatech Corporation	Human skin equivalents expressing exogenous polypeptides
BRPI0513826A2 (pt)	2004-07-26	2010-06-22	Dow Global Technologies Inc	processo para expressão de proteìna melhorada através de engenharia de cepa
JP4993752B2 (ja)	2005-03-01	2012-08-08	ストラタテックコーポレーション	外因性ポリペプチドを発現するヒト皮膚同等物
JP2008532550A (ja)	2005-03-17	2008-08-21	ストラタテックコーポレーション	純度が改良された代用皮膚
EP3138403A1 (en)	2005-08-09	2017-03-08	Revivicor, Inc.	Transgenic ungulates expressing ctla4-ig and uses thereof
US9580719B2 (en)	2007-04-27	2017-02-28	Pfenex, Inc.	Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9394571B2 (en)	2007-04-27	2016-07-19	Pfenex Inc.	Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
EP2152231A1 (en) *	2007-06-11	2010-02-17	Chanel Parfums Beauté	Use of active agents for stimulating the expression of fn3k and/or fn3k rp for combating ageing of the skin
WO2009065005A1 (en)	2007-11-14	2009-05-22	Stratatech Corporation	Cold storage of organotypically cultured skin equivalents for clinical applications
PT2352472T (pt)	2008-11-04	2017-04-11	Stratatech Corp	Equivalentes de pele secos e irradiados prontos a utilizar
WO2010065907A2 (en) *	2008-12-05	2010-06-10	The United States Of America As Represented By The Secretary, Department Of Health And Human Services	Use of a rock inhibitor to sustain primary human keratinocytes in a proliferative state
WO2010135655A2 (en)	2009-05-21	2010-11-25	Stratatech Corporation	Human skin substitutes expressing il-12
KR101055320B1 (ko)	2009-11-25	2011-08-08	서울대학교산학협력단	자발적으로 불멸화 또는 종양화가 유도된 신규 세포주 및 이의 용도
CA3071928A1 (en)	2013-03-13	2014-10-02	Stratatech Corporation	Cryopreservation of viable human skin substitutes
EP2991692B1 (en)	2013-05-03	2019-08-21	The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.	Skin substitutes and methods for hair follicle neogenesis
TWI868601B (zh) *	2022-02-24	2025-01-01	財團法人國家衛生研究院	人類皮膚仿生單元及其製備方法
EP4257158A1 (en) *	2022-04-06	2023-10-11	Servicio Andaluz de Salud	Biomimetic model of non-melanoma skin cancer and uses thereof

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US2663907A (en) *	1949-02-14	1953-12-29	British Celanese	Converting fibrous cellulose into an easily powderable form
US2636879A (en) *	1950-04-27	1953-04-28	Du Pont	Manufacture of cellulose ethers
US4455237A (en) *	1982-01-05	1984-06-19	James River Corporation	High bulk pulp, filter media utilizing such pulp, related processes
DE3833045A1 (de) *	1988-09-29	1990-04-05	Henkel Kgaa	Bohrspueladditive auf basis eines polymergemiches, deren verwendung sowie verfahren zur herstellung derselben
US5491084A (en) *	1993-09-10	1996-02-13	The Trustees Of Columbia University In The City Of New York	Uses of green-fluorescent protein
DE19632865A1 (de) *	1996-08-14	1998-02-19	Giesecke & Devrient Gmbh	Verfahren zur Herstellung von Papiermasse
US6174412B1 (en) *	1998-03-02	2001-01-16	Purely Cotton, Inc.	Cotton linter tissue products and method for preparing same
US5989837A (en) *	1998-07-13	1999-11-23	Wisconsin Alumni Research Foundation	Immortalized human keratinocyte cell line
EP1334132A1 (en) *	2000-11-01	2003-08-13	BKI Holding Corporation	Cellulose ethers and method of preparing the same

1998
- 1998-07-13 US US09/114,557 patent/US5989837A/en not_active Expired - Lifetime
1999
- 1999-03-26 US US09/277,295 patent/US6214567B1/en not_active Expired - Lifetime
- 1999-07-12 BR BRPI9912047 patent/BRPI9912047B8/pt not_active IP Right Cessation
- 1999-07-12 EP EP99933896A patent/EP1117423B1/en not_active Expired - Lifetime
- 1999-07-12 CN CNB998085642A patent/CN1197960C/zh not_active Expired - Lifetime
- 1999-07-12 IL IL140608A patent/IL140608A/en not_active IP Right Cessation
- 1999-07-12 JP JP2000559428A patent/JP3557172B2/ja not_active Expired - Lifetime
- 1999-07-12 AT AT06003693T patent/ATE550439T1/de active
- 1999-07-12 KR KR10-2001-7000430A patent/KR100461853B1/ko not_active Expired - Lifetime
- 1999-07-12 DK DK99933896T patent/DK1117423T3/da active
- 1999-07-12 ES ES99933896T patent/ES2267277T3/es not_active Expired - Lifetime
- 1999-07-12 WO PCT/US1999/015671 patent/WO2000003244A2/en not_active Ceased
- 1999-07-12 EP EP06003693A patent/EP1693066B1/en not_active Expired - Lifetime
- 1999-07-12 AU AU49850/99A patent/AU758141B2/en not_active Expired
- 1999-07-12 CA CA2336717A patent/CA2336717C/en not_active Expired - Lifetime
- 1999-07-12 DE DE69931941T patent/DE69931941T2/de not_active Expired - Lifetime
- 1999-07-12 AT AT99933896T patent/ATE329611T1/de not_active IP Right Cessation
- 1999-07-12 ES ES06003693T patent/ES2384264T3/es not_active Expired - Lifetime
- 1999-07-12 NZ NZ509184A patent/NZ509184A/xx not_active IP Right Cessation
2001
- 2001-01-24 US US09/769,124 patent/US6485724B2/en not_active Expired - Lifetime
- 2001-04-27 US US09/844,194 patent/US6495135B2/en not_active Expired - Lifetime
- 2001-08-31 US US09/945,136 patent/US6514711B2/en not_active Expired - Lifetime
2002
- 2002-11-19 US US10/299,938 patent/US6884595B2/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
US20030157476A1 (en)	2003-08-21
ATE550439T1 (de)	2012-04-15
JP2002520594A (ja)	2002-07-09
DK1117423T3 (da)	2006-10-16
US6495135B2 (en)	2002-12-17
US6884595B2 (en)	2005-04-26
KR100461853B1 (ko)	2004-12-14
ATE329611T1 (de)	2006-07-15
IL140608A (en)	2006-06-11
JP3557172B2 (ja)	2004-08-25
KR20010071839A (ko)	2001-07-31
WO2000003244A2 (en)	2000-01-20
AU758141B2 (en)	2003-03-13
EP1693066B1 (en)	2012-03-21
WO2000003244A9 (en)	2000-03-23
EP1117423A2 (en)	2001-07-25
EP1693066A3 (en)	2007-11-21
AU4985099A (en)	2000-02-01
CN1326354A (zh)	2001-12-12
ES2384264T3 (es)	2012-07-03
DE69931941T2 (de)	2007-02-08
US20020102726A1 (en)	2002-08-01
CA2336717C (en)	2012-05-22
EP1117423B1 (en)	2006-06-14
BRPI9912047B8 (pt)	2021-07-06
ES2267277T3 (es)	2007-03-01
CN1197960C (zh)	2005-04-20
US6214567B1 (en)	2001-04-10
BRPI9912047A (pt)	2001-04-03
US5989837A (en)	1999-11-23
CA2336717A1 (en)	2000-01-20
EP1693066A2 (en)	2006-08-23
IL140608A0 (en)	2002-02-10
NZ509184A (en)	2003-01-31
US20010023061A1 (en)	2001-09-20
US6514711B2 (en)	2003-02-04
DE69931941D1 (de)	2006-07-27
WO2000003244A3 (en)	2001-04-26
US20020142282A1 (en)	2002-10-03
US20040265787A9 (en)	2004-12-30
US6485724B2 (en)	2002-11-26

Legal Events

Date	Code	Title	Description
2014-03-18	B16A	Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]	Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 18/03/2014, OBSERVADAS AS CONDICOES LEGAIS.
2021-07-06	B16C	Correction of notification of the grant [chapter 16.3 patent gazette]	Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 12/07/99, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO
2024-05-28	B21A	Patent or certificate of addition expired [chapter 21.1 patent gazette]	Free format text: PATENTE EXTINTA EM 12/07/2019

Publication	Publication Date	Title
BRPI9912047B1 (pt)	2014-03-18	Métodos para avaliar células malignas em uma epiderme in vitro, e para avaliar compostos de teste quanto à toxicidade humana.
WO1999045386A8 (en)	2000-04-13	Method for performing blood cell counts
DE69733629D1 (de)	2005-08-04	Verfahren zum nachweis solider krebszellen und histologischer heterotypien und verfahren zur prüfung von gewebe für die knochenmarktransplantation und die transplantation von peripheren blutstammzellen
DE69837529D1 (de)	2007-05-24	Proteinmarker für lungenkrebs und deren verwendung
ATE512233T1 (de)	2011-06-15	Screeningverfahren für die aktivität von agenzien unter verwendung von teleosten
DE69931892D1 (de)	2006-07-27	Test für antibiotikaresistenz
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