CA1048409A - Methode pour ameliorer le contraste dans les epreuves immunologiques avec des proteines a grosses molecules - Google Patents

Methode pour ameliorer le contraste dans les epreuves immunologiques avec des proteines a grosses molecules

Info

Publication number: CA1048409A
Authority: CA; Canada
Prior art keywords: protein; solution; substrate; antigen; molecules
Prior art date: 1973-08-30
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA74207341A

Other languages

English (en)

Other versions

CA207341S (en

Inventor

Ivar Giaever

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

General Electric Co

Original Assignee

General Electric Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1973-08-30

Filing date

1974-08-19

Publication date

1979-02-13

1974-08-19 Application filed by General Electric Co filed Critical General Electric Co

1979-02-13 Application granted granted Critical

1979-02-13 Publication of CA1048409A publication Critical patent/CA1048409A/fr

Status Expired legal-status Critical Current

Links

102000004169 proteins and genes Human genes 0.000 title claims abstract description 166
108090000623 proteins and genes Proteins 0.000 title claims abstract description 166
238000000034 method Methods 0.000 title claims abstract description 38
230000001900 immune effect Effects 0.000 title claims abstract description 8
239000000758 substrate Substances 0.000 claims abstract description 154
102000036639 antigens Human genes 0.000 claims abstract description 129
108091007433 antigens Proteins 0.000 claims abstract description 128
239000000427 antigen Substances 0.000 claims abstract description 93
210000002966 serum Anatomy 0.000 claims description 18
238000000576 coating method Methods 0.000 claims description 9
230000008105 immune reaction Effects 0.000 claims description 9
239000011248 coating agent Substances 0.000 claims description 8
239000002052 molecular layer Substances 0.000 claims description 5
238000000151 deposition Methods 0.000 claims description 2
238000007654 immersion Methods 0.000 abstract description 7
238000001179 sorption measurement Methods 0.000 abstract description 6
230000008859 change Effects 0.000 abstract description 4
239000010410 layer Substances 0.000 description 103
239000000243 solution Substances 0.000 description 55
229910052751 metal Inorganic materials 0.000 description 23
239000002184 metal Substances 0.000 description 23
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 13
229910052737 gold Inorganic materials 0.000 description 13
239000010931 gold Substances 0.000 description 13
102000004190 Enzymes Human genes 0.000 description 12
108090000790 Enzymes Proteins 0.000 description 12
241000700605 Viruses Species 0.000 description 10
238000001514 detection method Methods 0.000 description 10
239000011521 glass Substances 0.000 description 10
208000006454 hepatitis Diseases 0.000 description 9
231100000283 hepatitis Toxicity 0.000 description 8
APFVFJFRJDLVQX-UHFFFAOYSA-N indium atom Chemical compound [In] APFVFJFRJDLVQX-UHFFFAOYSA-N 0.000 description 7
230000003287 optical effect Effects 0.000 description 7
229910052738 indium Inorganic materials 0.000 description 6
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
239000002245 particle Substances 0.000 description 5
241000282414 Homo sapiens Species 0.000 description 4
230000000890 antigenic effect Effects 0.000 description 3
239000005350 fused silica glass Substances 0.000 description 3
QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 3
229910052753 mercury Inorganic materials 0.000 description 3
108090000765 processed proteins & peptides Proteins 0.000 description 3
230000035945 sensitivity Effects 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
241001465754 Metazoa Species 0.000 description 2
238000013019 agitation Methods 0.000 description 2
150000001413 amino acids Chemical class 0.000 description 2
230000008901 benefit Effects 0.000 description 2
239000003990 capacitor Substances 0.000 description 2
230000000295 complement effect Effects 0.000 description 2
230000000536 complexating effect Effects 0.000 description 2
238000001704 evaporation Methods 0.000 description 2
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
239000000463 material Substances 0.000 description 2
239000010445 mica Substances 0.000 description 2
229910052618 mica group Inorganic materials 0.000 description 2
239000004033 plastic Substances 0.000 description 2
229940070376 protein Drugs 0.000 description 2
150000003839 salts Chemical class 0.000 description 2
102000009027 Albumins Human genes 0.000 description 1
108010088751 Albumins Proteins 0.000 description 1
229910000497 Amalgam Inorganic materials 0.000 description 1
241000256844 Apis mellifera Species 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
108060003951 Immunoglobulin Proteins 0.000 description 1
102000004877 Insulin Human genes 0.000 description 1
108090001061 Insulin Proteins 0.000 description 1
BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
241000251539 Vertebrata <Metazoa> Species 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
238000005842 biochemical reaction Methods 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
239000008280 blood Substances 0.000 description 1
238000006243 chemical reaction Methods 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
239000000470 constituent Substances 0.000 description 1
238000002405 diagnostic procedure Methods 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
230000005670 electromagnetic radiation Effects 0.000 description 1
230000008020 evaporation Effects 0.000 description 1
-1 for example Inorganic materials 0.000 description 1
102000018358 immunoglobulin Human genes 0.000 description 1
229940072221 immunoglobulins Drugs 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
238000007689 inspection Methods 0.000 description 1
229940125396 insulin Drugs 0.000 description 1
230000009545 invasion Effects 0.000 description 1
150000002605 large molecules Chemical class 0.000 description 1
239000011133 lead Substances 0.000 description 1
229920002521 macromolecule Polymers 0.000 description 1
238000005065 mining Methods 0.000 description 1
239000000203 mixture Substances 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000008520 organization Effects 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
230000035935 pregnancy Effects 0.000 description 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
230000008569 process Effects 0.000 description 1
238000000746 purification Methods 0.000 description 1
239000010453 quartz Substances 0.000 description 1
238000002310 reflectometry Methods 0.000 description 1
230000004044 response Effects 0.000 description 1
229910052709 silver Inorganic materials 0.000 description 1
239000004332 silver Substances 0.000 description 1
239000002356 single layer Substances 0.000 description 1
239000000126 substance Substances 0.000 description 1
229910052715 tantalum Inorganic materials 0.000 description 1
GUVRBAGPIYLISA-UHFFFAOYSA-N tantalum atom Chemical compound [Ta] GUVRBAGPIYLISA-UHFFFAOYSA-N 0.000 description 1
229910052718 tin Inorganic materials 0.000 description 1
239000011135 tin Substances 0.000 description 1
210000002700 urine Anatomy 0.000 description 1
238000001429 visible spectrum Methods 0.000 description 1
230000000007 visual effect Effects 0.000 description 1

Landscapes

Investigating Or Analysing Biological Materials (AREA)

CA74207341A 1973-08-30 1974-08-19 Methode pour ameliorer le contraste dans les epreuves immunologiques avec des proteines a grosses molecules Expired CA1048409A (fr)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US39295173A	1973-08-30	1973-08-30

Publications (1)

Publication Number	Publication Date
CA1048409A true CA1048409A (fr)	1979-02-13

Family

ID=23552681

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA74207341A Expired CA1048409A (fr)	1973-08-30	1974-08-19	Methode pour ameliorer le contraste dans les epreuves immunologiques avec des proteines a grosses molecules

Country Status (1)

Country	Link
CA (1)	CA1048409A (fr)

1974
- 1974-08-19 CA CA74207341A patent/CA1048409A/fr not_active Expired

Publication	Publication Date	Title
CA1039649A (fr)	1978-10-03	Methode pour former des films d'un complexe immunologique en plusieurs couches
US3926564A (en)	1975-12-16	Substrate for immunological tests and method of fabrication thereof
US4054646A (en)	1977-10-18	Method and apparatus for detection of antibodies and antigens
US3960490A (en)	1976-06-01	Method and apparatus for detecting immunologic reactions by diffusion in gel
EP0254575B1 (fr)	1993-03-10	Structures optiques recouvertes par des polymères
US3979509A (en)	1976-09-07	Opaque layer method for detecting biological particles
US3960488A (en)	1976-06-01	Method and apparatus for quantitative surface inhibition test
CA1339409C (fr)	1997-09-02	Structures optiques enduites de polymere
EP0553229B1 (fr)	1996-02-07	Amelioration relative au dosage faisant appel a une liaison en phase solide
US4820649A (en)	1989-04-11	Method and kit having layered device for detecting biological component by interference color
US3979184A (en)	1976-09-07	Diagnostic device for visually detecting presence of biological particles
TW594010B (en)	2004-06-21	Internal calibration system for flow-through assays
JPS5916669B2 (ja)	1984-04-17	抗原又は抗体の検出方法
US8158440B2 (en)	2012-04-17	Method for quantitative measurement of thyroid related antibodies or antigens in a serum sample
JPS5944583B2 (ja)	1984-10-30	蛋白質の検出法
US5529901A (en)	1996-06-25	Method for determining the presence or amount of analyte using a stable colloidal carbon sol
CA1048409A (fr)	1979-02-13	Methode pour ameliorer le contraste dans les epreuves immunologiques avec des proteines a grosses molecules
JP4517081B2 (ja)	2010-08-04	免疫センサ用デバイス
EP1497652B1 (fr)	2007-11-14	Methode, systeme et kit permettant de detecter un analyte dans un echantillon
GB1564333A (en)	1980-04-10	Method for contrast in surface immunological tests
CA1049398A (fr)	1979-02-27	Methode et appareil pour detecter les particules biologiques ayant des proprietes immunologiques
IL87393A (en)	1994-06-24	Test strips for the YASSAONUMMI method for multiple analysts, diagnostic kits that include them and YASSAONUMMI methods using them
Kawaguchi et al.	1991	A highly sensitive device for visual detection of antigens and antibodies by means of light interference
CA1049401A (fr)	1979-02-27	Methode et appareil pour detecter les reactions immunologiques par diffusion dans un gel
US20240310373A1 (en)	2024-09-19	Biosensor, method of making the same, and molecule detection method