CA1059901A - Dosage radioimmunologique direct des antigenes et des anticorps - Google Patents

Dosage radioimmunologique direct des antigenes et des anticorps

Info

Publication number: CA1059901A
Authority: CA; Canada
Prior art keywords: antibody; antigen; test apparatus; incubated; layer
Prior art date: 1974-06-17
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA225,016A

Other languages

English (en)

Other versions

CA225016S (en

Inventor

Chung-Mei Ling

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Abbott Laboratories

Original Assignee

Abbott Laboratories

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1974-06-17

Filing date

1975-04-10

Publication date

1979-08-07

1974-06-17 Priority claimed from US05/480,180 external-priority patent/US4012494A/en

1975-04-10 Application filed by Abbott Laboratories filed Critical Abbott Laboratories

1979-08-07 Application granted granted Critical

1979-08-07 Publication of CA1059901A publication Critical patent/CA1059901A/fr

Status Expired legal-status Critical Current

Links

239000000427 antigen Substances 0.000 title claims abstract description 73
102000036639 antigens Human genes 0.000 title claims abstract description 73
108091007433 antigens Proteins 0.000 title claims abstract description 73
238000003127 radioimmunoassay Methods 0.000 title claims abstract description 17
238000000034 method Methods 0.000 claims abstract description 49
238000012360 testing method Methods 0.000 claims abstract description 42
238000000576 coating method Methods 0.000 claims abstract description 16
238000005406 washing Methods 0.000 claims abstract description 16
239000011248 coating agent Substances 0.000 claims abstract description 14
239000000203 mixture Substances 0.000 claims abstract description 10
230000005855 radiation Effects 0.000 claims abstract description 9
239000000523 sample Substances 0.000 claims description 26
208000006454 hepatitis Diseases 0.000 claims description 22
231100000283 hepatitis Toxicity 0.000 claims description 22
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
238000011534 incubation Methods 0.000 claims description 20
239000000463 material Substances 0.000 claims description 14
239000000243 solution Substances 0.000 claims description 13
239000000872 buffer Substances 0.000 claims description 11
239000008367 deionised water Substances 0.000 claims description 11
229910021641 deionized water Inorganic materials 0.000 claims description 10
YZCKVEUIGOORGS-NJFSPNSNSA-N Tritium Chemical compound [3H] YZCKVEUIGOORGS-NJFSPNSNSA-N 0.000 claims description 2
229910052722 tritium Inorganic materials 0.000 claims description 2
230000002285 radioactive effect Effects 0.000 claims 3
239000008366 buffered solution Substances 0.000 claims 2
239000013068 control sample Substances 0.000 claims 1
239000013014 purified material Substances 0.000 claims 1
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 abstract description 24
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 abstract description 12
210000002966 serum Anatomy 0.000 abstract description 10
238000003556 assay Methods 0.000 abstract 1
210000002381 plasma Anatomy 0.000 description 15
210000004369 blood Anatomy 0.000 description 7
239000008280 blood Substances 0.000 description 7
241000700605 Viruses Species 0.000 description 6
239000012153 distilled water Substances 0.000 description 5
238000010998 test method Methods 0.000 description 4
241000894006 Bacteria Species 0.000 description 2
239000013566 allergen Substances 0.000 description 2
239000011324 bead Substances 0.000 description 2
239000012888 bovine serum Substances 0.000 description 2
210000000234 capsid Anatomy 0.000 description 2
210000000170 cell membrane Anatomy 0.000 description 2
238000002405 diagnostic procedure Methods 0.000 description 2
208000002672 hepatitis B Diseases 0.000 description 2
229940088597 hormone Drugs 0.000 description 2
239000005556 hormone Substances 0.000 description 2
-1 i.e. Chemical compound 0.000 description 2
ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
RZZPDXZPRHQOCG-OJAKKHQRSA-O CDP-choline(1+) Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OCC[N+](C)(C)C)O[C@H]1N1C(=O)N=C(N)C=C1 RZZPDXZPRHQOCG-OJAKKHQRSA-O 0.000 description 1
206010019799 Hepatitis viral Diseases 0.000 description 1
239000004743 Polypropylene Substances 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
101000874347 Streptococcus agalactiae IgA FC receptor Proteins 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
238000001514 detection method Methods 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
238000007598 dipping method Methods 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
230000000694 effects Effects 0.000 description 1
108010074605 gamma-Globulins Proteins 0.000 description 1
239000012535 impurity Substances 0.000 description 1
229910052740 iodine Inorganic materials 0.000 description 1
239000011630 iodine Substances 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
229920002521 macromolecule Polymers 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
239000013642 negative control Substances 0.000 description 1
239000002245 particle Substances 0.000 description 1
239000004033 plastic Substances 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
229920001155 polypropylene Polymers 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
239000012857 radioactive material Substances 0.000 description 1
239000000700 radioactive tracer Substances 0.000 description 1
239000007787 solid Substances 0.000 description 1
239000007790 solid phase Substances 0.000 description 1
201000001862 viral hepatitis Diseases 0.000 description 1
238000010792 warming Methods 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Immunology (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Chemical & Material Sciences (AREA)
Biomedical Technology (AREA)
Urology & Nephrology (AREA)
Hematology (AREA)
Biotechnology (AREA)
Analytical Chemistry (AREA)
Cell Biology (AREA)
Communicable Diseases (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Investigating Or Analysing Biological Materials (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)

CA225,016A 1974-06-17 1975-04-10 Dosage radioimmunologique direct des antigenes et des anticorps Expired CA1059901A (fr)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US05/480,180 US4012494A (en)	1971-12-21	1974-06-17	Direct radioimmunoassay for antigens and their antibodies

Publications (1)

Publication Number	Publication Date
CA1059901A true CA1059901A (fr)	1979-08-07

Family

ID=23906963

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA225,016A Expired CA1059901A (fr)	1974-06-17	1975-04-10	Dosage radioimmunologique direct des antigenes et des anticorps

Country Status (3)

Country	Link
CA (1)	CA1059901A (fr)
DE (1)	DE2526800A1 (fr)
FR (1)	FR2274687A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
FR2418457A1 (fr) *	1978-02-22	1979-09-21	Commissariat Energie Atomique	Dispositif pour analyser une solution
FR2495326B1 (fr) *	1980-12-03	1986-03-28	Georges Desmonts	Procede de dosage immunologique, du type a agglutination, pour deceler des anticorps de type immunoglobuline
CA1172561A (fr) *	1981-01-28	1984-08-14	Richard H. Decker	Methode de detection d'immunoglobulines

1975
- 1975-04-10 CA CA225,016A patent/CA1059901A/fr not_active Expired
- 1975-06-16 FR FR7518785A patent/FR2274687A1/fr active Granted
- 1975-06-16 DE DE19752526800 patent/DE2526800A1/de active Pending

Also Published As

Publication number	Publication date
FR2274687B1 (fr)	1980-05-16
FR2274687A1 (fr)	1976-01-09
DE2526800A1 (de)	1976-01-02

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