CA1271705A - Method for the identification of nucleic acids - Google Patents

Method for the identification of nucleic acids

Info

Publication number: CA1271705A
Authority: CA; Canada
Prior art keywords: probe; nucleic acid; hybridization; target nucleic; affinity pair
Prior art date: 1985-01-02
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

CA000498834A

Other languages

English (en)

French (fr)

Inventor

Hans Soderlund

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

SANGTEC MOLECULAR DIAGNOSTICS AB

Original Assignee

Orion Yhtyma Oy

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-01-02

Filing date

1985-12-31

Publication date

1990-07-17

1985-12-31 Application filed by Orion Yhtyma Oy filed Critical Orion Yhtyma Oy

1990-07-17 Application granted granted Critical

1990-07-17 Publication of CA1271705A publication Critical patent/CA1271705A/en

2007-07-17 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

150000007523 nucleic acids Chemical class 0.000 title claims abstract description 37
108020004707 nucleic acids Proteins 0.000 title claims abstract description 36
102000039446 nucleic acids Human genes 0.000 title claims abstract description 36
238000000034 method Methods 0.000 title claims abstract description 32
239000000523 sample Substances 0.000 claims abstract description 70
238000009396 hybridization Methods 0.000 claims abstract description 41
239000007787 solid Substances 0.000 claims description 5
229910001385 heavy metal Inorganic materials 0.000 claims description 2
239000000427 antigen Substances 0.000 claims 1
102000036639 antigens Human genes 0.000 claims 1
108091007433 antigens Proteins 0.000 claims 1
125000004149 thio group Chemical group *S* 0.000 claims 1
108020004414 DNA Proteins 0.000 description 21
239000000243 solution Substances 0.000 description 21
239000013612 plasmid Substances 0.000 description 14
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 12
210000004027 cell Anatomy 0.000 description 10
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
239000012634 fragment Substances 0.000 description 8
239000000203 mixture Substances 0.000 description 8
238000012360 testing method Methods 0.000 description 7
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
239000000872 buffer Substances 0.000 description 4
239000011780 sodium chloride Substances 0.000 description 4
239000001488 sodium phosphate Substances 0.000 description 4
229910000162 sodium phosphate Inorganic materials 0.000 description 4
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 4
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
108010090804 Streptavidin Proteins 0.000 description 3
239000007853 buffer solution Substances 0.000 description 3
230000000694 effects Effects 0.000 description 3
239000000463 material Substances 0.000 description 3
229910052708 sodium Inorganic materials 0.000 description 3
239000011734 sodium Substances 0.000 description 3
235000019333 sodium laurylsulphate Nutrition 0.000 description 3
239000000126 substance Substances 0.000 description 3
241000606153 Chlamydia trachomatis Species 0.000 description 2
241000588724 Escherichia coli Species 0.000 description 2
LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
229920002684 Sepharose Polymers 0.000 description 2
239000002253 acid Substances 0.000 description 2
238000001042 affinity chromatography Methods 0.000 description 2
229960002685 biotin Drugs 0.000 description 2
235000020958 biotin Nutrition 0.000 description 2
239000011616 biotin Substances 0.000 description 2
238000009835 boiling Methods 0.000 description 2
229940038705 chlamydia trachomatis Drugs 0.000 description 2
230000001419 dependent effect Effects 0.000 description 2
-1 heme compounds Chemical class 0.000 description 2
238000011534 incubation Methods 0.000 description 2
108090000623 proteins and genes Proteins 0.000 description 2
230000002285 radioactive effect Effects 0.000 description 2
238000011160 research Methods 0.000 description 2
241000701161 unidentified adenovirus Species 0.000 description 2
238000005406 washing Methods 0.000 description 2
XZKIHKMTEMTJQX-UHFFFAOYSA-N 4-Nitrophenyl Phosphate Chemical compound OP(O)(=O)OC1=CC=C([N+]([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-N 0.000 description 1
BRLRJZRHRJEWJY-VCOUNFBDSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-n-[3-[3-(4-azido-2-nitroanilino)propyl-methylamino]propyl]pentanamide Chemical compound C([C@H]1[C@H]2NC(=O)N[C@H]2CS1)CCCC(=O)NCCCN(C)CCCNC1=CC=C(N=[N+]=[N-])C=C1[N+]([O-])=O BRLRJZRHRJEWJY-VCOUNFBDSA-N 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
241000726103 Atta Species 0.000 description 1
108090001008 Avidin Proteins 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
108010008286 DNA nucleotidylexotransferase Proteins 0.000 description 1
102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 description 1
229920002307 Dextran Polymers 0.000 description 1
SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
102000008857 Ferritin Human genes 0.000 description 1
108050000784 Ferritin Proteins 0.000 description 1
238000008416 Ferritin Methods 0.000 description 1
241000701109 Human adenovirus 2 Species 0.000 description 1
102000016943 Muramidase Human genes 0.000 description 1
108010014251 Muramidase Proteins 0.000 description 1
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
239000008118 PEG 6000 Substances 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
229920002584 Polyethylene Glycol 6000 Polymers 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
229920004890 Triton X-100 Polymers 0.000 description 1
239000013504 Triton X-100 Substances 0.000 description 1
JGQHTPYIIXRFLR-UHFFFAOYSA-N [2-(9h-fluoren-1-yl)-2-oxoethyl] acetate Chemical class C1C2=CC=CC=C2C2=C1C(C(=O)COC(=O)C)=CC=C2 JGQHTPYIIXRFLR-UHFFFAOYSA-N 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
101150115889 al gene Proteins 0.000 description 1
230000003321 amplification Effects 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
244000309466 calf Species 0.000 description 1
239000012876 carrier material Substances 0.000 description 1
239000013592 cell lysate Substances 0.000 description 1
239000001913 cellulose Substances 0.000 description 1
229920002678 cellulose Polymers 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
229960005091 chloramphenicol Drugs 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
ZBCBWPMODOFKDW-UHFFFAOYSA-N diethanolamine Chemical compound OCCNCCO ZBCBWPMODOFKDW-UHFFFAOYSA-N 0.000 description 1
238000010828 elution Methods 0.000 description 1
238000000605 extraction Methods 0.000 description 1
238000005194 fractionation Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
210000004408 hybridoma Anatomy 0.000 description 1
ATADHKWKHYVBTJ-UHFFFAOYSA-N hydron;4-[1-hydroxy-2-(methylamino)ethyl]benzene-1,2-diol;chloride Chemical compound Cl.CNCC(O)C1=CC=C(O)C(O)=C1 ATADHKWKHYVBTJ-UHFFFAOYSA-N 0.000 description 1
WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
230000001900 immune effect Effects 0.000 description 1
238000010348 incorporation Methods 0.000 description 1
208000015181 infectious disease Diseases 0.000 description 1
229910052747 lanthanoid Inorganic materials 0.000 description 1
150000002602 lanthanoids Chemical class 0.000 description 1
239000004816 latex Substances 0.000 description 1
229920000126 latex Polymers 0.000 description 1
239000003446 ligand Substances 0.000 description 1
229960000274 lysozyme Drugs 0.000 description 1
239000004325 lysozyme Substances 0.000 description 1
235000010335 lysozyme Nutrition 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
239000010452 phosphate Substances 0.000 description 1
229920003023 plastic Polymers 0.000 description 1
239000004033 plastic Substances 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229920000573 polyethylene Polymers 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
239000012488 sample solution Substances 0.000 description 1
239000001509 sodium citrate Substances 0.000 description 1
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
238000000527 sonication Methods 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
239000000758 substrate Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
210000001541 thymus gland Anatomy 0.000 description 1
238000013519 translation Methods 0.000 description 1
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)
Saccharide Compounds (AREA)

CA000498834A 1985-01-02 1985-12-31 Method for the identification of nucleic acids Expired - Lifetime CA1271705A (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
FI850004		1985-01-02
FI850004A FI72146C (fi)	1985-01-02	1985-01-02	Foerfarande foer identifiering av nukleinsyror.

Publications (1)

Publication Number	Publication Date
CA1271705A true CA1271705A (en)	1990-07-17

Family

ID=8520136

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA000498834A Expired - Lifetime CA1271705A (en)	1985-01-02	1985-12-31	Method for the identification of nucleic acids

Country Status (21)

Country	Link
JP (1)	JPH0669400B2 (de)
AT (1)	AT397514B (de)
AU (1)	AU561382B2 (de)
BE (1)	BE903937A (de)
CA (1)	CA1271705A (de)
CH (1)	CH666696A5 (de)
DE (1)	DE3546312A1 (de)
DK (1)	DK164932C (de)
FI (1)	FI72146C (de)
FR (1)	FR2575493B1 (de)
GB (1)	GB2169403B (de)
HU (1)	HU196453B (de)
IE (1)	IE58290B1 (de)
IL (1)	IL77489A (de)
IT (1)	IT1201514B (de)
LU (1)	LU86238A1 (de)
NL (1)	NL189427C (de)
NO (1)	NO166743C (de)
RO (1)	RO94651B (de)
SE (1)	SE463212B (de)
ZA (1)	ZA859895B (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN1303221C (zh) *	2003-01-27	2007-03-07	英科新创(厦门)科技有限公司	使用亲和放大的集成信号基团的靶核酸检测法

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5288609A (en) *	1984-04-27	1994-02-22	Enzo Diagnostics, Inc.	Capture sandwich hybridization method and composition
US6060237A (en)	1985-02-26	2000-05-09	Biostar, Inc.	Devices and methods for optical detection of nucleic acid hybridization
IL79112A (en) †	1985-06-13	1992-01-15	Abbott Lab	Method for the isolation of a nucleic acid sequence and kit for performing a hybridization assay
US4868105A (en) *	1985-12-11	1989-09-19	Chiron Corporation	Solution phase nucleic acid sandwich assay
US4910300A (en) *	1985-12-11	1990-03-20	Chiron Corporation	Method for making nucleic acid probes
US4882269A (en) *	1985-12-13	1989-11-21	Princeton University	Amplified hybridization assay
FI76119C (fi) *	1986-02-27	1988-09-09	Orion Yhtymae Oy	Kvantitativ bestaemning av nukleinsyramolekyler och reagensfoerpackning som anvaends vid foerfarandet.
AU622104B2 (en) *	1987-03-11	1992-04-02	Sangtec Molecular Diagnostics Ab	Method of assaying of nucleic acids, a reagent combination and kit therefore
WO1988010313A1 (en) *	1987-06-26	1988-12-29	E.I. Du Pont De Nemours And Company	Affinity removal of contaminating sequences from recombinant cloned na using capture beads
WO1989001049A1 (en) *	1987-07-31	1989-02-09	Gen-Probe Incorporated	Assay for polynucleotides employing oligonucleotides to eliminate undesirable cross reactions
EP0305145A3 (de) *	1987-08-24	1990-05-02	Ortho Diagnostic Systems Inc.	Verfahren und Sonden zur Bestimmung von Nukleinsäuren
EP0304845A3 (de) *	1987-08-28	1991-03-06	Profile Diagnostic Sciences Inc.	Verfahren und Zusammensetzung zum Nachweis der Genexpression
CA1325761C (en) *	1987-12-25	1994-01-04	Takanori Oka	Method of detecting an intended nucleic acid in a sample
DE3800644A1 (de) *	1988-01-12	1989-07-20	Boehringer Mannheim Gmbh	Verfahren zum hochspezifischen nachweis von nukleinsaeuren in loesung
US5104791A (en) *	1988-02-09	1992-04-14	E. I. Du Pont De Nemours And Company	Particle counting nucleic acid hybridization assays
EP0436547B1 (de) *	1988-05-10	1994-11-30	E.I. Du Pont De Nemours And Company	Verfahren zum schnellen nachweis von nukleinsäuren
US5185243A (en) *	1988-08-25	1993-02-09	Syntex (U.S.A.) Inc.	Method for detection of specific nucleic acid sequences
US5858652A (en) *	1988-08-30	1999-01-12	Abbott Laboratories	Detection and amplification of target nucleic acid sequences
AU629845B2 (en) *	1988-08-30	1992-10-15	Abbott Laboratories	Detection and amplification of target nucleic acid sequences
GB2225112A (en) *	1988-11-22	1990-05-23	Ici Plc	Hybridisation probes
US5082935A (en) *	1988-12-15	1992-01-21	Amoco Corporation	Diagnostic reagents made by attaching cytidine containing nucleic acid probes to amino functionalized solid supports by bisulfite mediated transamination
JP3015462B2 (ja) *	1989-02-06	2000-03-06	ジーン‐トラック・システムス	リステリア検出のためのプローブ類及び方法
JP3046837B2 (ja)	1989-03-10	2000-05-29	バイシス・インコーポレーテツド	固定化されたオリゴヌクレオチドのプローブおよびそれらの使用
CA2049042A1 (en) *	1989-03-10	1990-09-11	Mark L. Collins	Preventing interference with affinity capture schemes
EP0484385A4 (en) *	1989-07-11	1993-05-26	Microprobe Corporation	Quantification of bacteria using a nucleic acid hybridization assay
US5334501A (en) *	1989-07-11	1994-08-02	Microprobe Corporation	Quantification of bacteria using a nucleic acid hybridization assay
GB8924989D0 (en) *	1989-11-06	1989-12-28	Scotgen Ltd	Method and device for the detection of antibiotic resistance
US5580970A (en) *	1989-12-01	1996-12-03	Amoco Corporation	Detection of HPV transcripts
AU7429591A (en) *	1990-04-18	1991-10-24	Gene-Trak Systems	Nucleic acid probes for the detection of giardia lamblia
EP0529070A1 (de) *	1991-02-27	1993-03-03	Amoco Corporation	Verfahren zur verbesserung der empfindlichkeit von hybridisierungsassays
WO1993020234A1 (en) *	1992-03-31	1993-10-14	E.I. Du Pont De Nemours And Company	A rapid, high capacity nucleic acid based assay
US7713528B1 (en)	1993-02-18	2010-05-11	Enzo Therapeutics, Inc.	Method for in vivo delivery of active compounds using reagent conjugate
RU2146707C1 (ru) *	1996-04-11	2000-03-20	Куликова Валентина Филипповна	Способ выявления анализируемой последовательности нуклеиновых кислот
US5853993A (en) *	1996-10-21	1998-12-29	Hewlett-Packard Company	Signal enhancement method and kit
GB0016833D0 (en)	2000-07-07	2000-08-30	Lee Helen	Improved dipstick assays (2)
US7465540B2 (en)	2000-09-21	2008-12-16	Luminex Corporation	Multiple reporter read-out for bioassays

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
FI63596C (fi) *	1981-10-16	1983-07-11	Orion Yhtymae Oy	Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
FR2518755B1 (fr) *	1981-12-23	1986-04-11	Pasteur Institut	Sonde contenant un acide nucleique modifie et reconnaissable par des anticorps specifiques et utilisation de cette sonde pour detecter et caracteriser une sequence d'adn homologue
GB8306426D0 (en) *	1983-03-09	1983-04-13	Malcolm A D B	Detecting polynucleotide sequence
CA1228811A (en) *	1983-05-05	1987-11-03	Robert G. Pergolizzi	Assay method utilizing polynucleotide sequences
NO843838L (no) *	1983-09-26	1985-03-27	Ortho Diagnostic Systems Inc	Fremgangsmaate for detektering av nukleinsyre
ZA849594B (en) *	1983-12-12	1985-08-28	Miles Lab	Nucleic acid hybridization assay employing antibodies to intercalation complexes
FR2558172B1 (fr) *	1984-01-16	1986-06-13	Inst Nat Sante Rech Med	Sonde contenant un acide nucleique modifie et reconnaissance par des anticorps specifiques et utilisation de cette sonde et de ces anticorps specifiques pour detecter et caracteriser une sequence d'adn homologue
CA1223222A (en) *	1984-02-22	1987-06-23	Nanibhushan Dattagupta	Immobilized nucleic acid-containing probes
FR2560298B1 (fr) *	1984-02-28	1988-07-15	Mors	Procede et dispositif de reglage avec precision de la chute de pression d'un fluide initialement sous pression elevee, tel qu'un fluide hydraulique par exemple alimentant un groupe hydraulique, et application du procede au dispositif dans un groupe ou une centrale hydraulique, en etant integre ou non, notamment a une table de machine ou a tout dispositif de support d'outillage
NZ211453A (en) *	1984-03-22	1989-01-06	Biotechnology Research Enterpr	Aryl azides, their preparation and use in the detection, localisation and isolation of polynucleotides
US4766062A (en) *	1984-05-07	1988-08-23	Allied Corporation	Displacement polynucleotide assay method and polynucleotide complex reagent therefor

1985
- 1985-01-02 FI FI850004A patent/FI72146C/fi not_active IP Right Cessation
- 1985-12-17 CH CH5378/85A patent/CH666696A5/de not_active IP Right Cessation
- 1985-12-20 GB GB08531414A patent/GB2169403B/en not_active Expired
- 1985-12-24 BE BE0/216059A patent/BE903937A/fr not_active IP Right Cessation
- 1985-12-27 NO NO855308A patent/NO166743C/no not_active IP Right Cessation
- 1985-12-27 JP JP60299797A patent/JPH0669400B2/ja not_active Expired - Lifetime
- 1985-12-29 HU HU855030A patent/HU196453B/hu unknown
- 1985-12-30 DE DE19853546312 patent/DE3546312A1/de active Granted
- 1985-12-30 RO RO121637A patent/RO94651B/ro unknown
- 1985-12-30 FR FR858519394A patent/FR2575493B1/fr not_active Expired - Lifetime
- 1985-12-30 IT IT23408/85A patent/IT1201514B/it active
- 1985-12-30 ZA ZA859895A patent/ZA859895B/xx unknown
- 1985-12-30 LU LU86238A patent/LU86238A1/fr unknown
- 1985-12-30 AT AT0376785A patent/AT397514B/de not_active IP Right Cessation
- 1985-12-31 AU AU51748/85A patent/AU561382B2/en not_active Expired
- 1985-12-31 IL IL77489A patent/IL77489A/xx not_active IP Right Cessation
- 1985-12-31 CA CA000498834A patent/CA1271705A/en not_active Expired - Lifetime
- 1985-12-31 IE IE333385A patent/IE58290B1/en not_active IP Right Cessation
- 1985-12-31 NL NLAANVRAGE8503597,A patent/NL189427C/xx not_active IP Right Cessation
1986
- 1986-01-02 SE SE8600011A patent/SE463212B/sv not_active IP Right Cessation
- 1986-01-02 DK DK000386A patent/DK164932C/da not_active IP Right Cessation

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CN1303221C (zh) *	2003-01-27	2007-03-07	英科新创(厦门)科技有限公司	使用亲和放大的集成信号基团的靶核酸检测法

Also Published As

Publication number	Publication date
SE8600011D0 (sv)	1986-01-02
DK386D0 (da)	1986-01-02
DK164932B (da)	1992-09-07
FI850004L (fi)	1986-07-03
ZA859895B (en)	1986-08-27
AU5174885A (en)	1986-07-17
AU561382B2 (en)	1987-05-07
IT1201514B (it)	1989-02-02
GB8531414D0 (en)	1986-02-05
FR2575493B1 (fr)	1990-01-26
HU196453B (en)	1988-11-28
IT8523408A0 (it)	1985-12-30
IL77489A (en)	1991-01-31
BE903937A (fr)	1986-04-16
CH666696A5 (de)	1988-08-15
FI850004A0 (fi)	1985-01-02
GB2169403A (en)	1986-07-09
LU86238A1 (fr)	1986-04-14
ATA376785A (de)	1993-09-15
FI72146B (fi)	1986-12-31
DE3546312C2 (de)	1992-08-06
SE8600011L (sv)	1986-07-03
DK164932C (da)	1993-01-25
DE3546312A1 (de)	1986-07-10
NO166743C (no)	1991-08-28
GB2169403B (en)	1988-06-08
FR2575493A1 (fr)	1986-07-04
AT397514B (de)	1994-04-25
JPH0669400B2 (ja)	1994-09-07
HUT40166A (en)	1986-11-28
RO94651A (ro)	1988-06-30
NL189427B (nl)	1992-11-02
IE853333L (en)	1986-07-02
SE463212B (sv)	1990-10-22
NL189427C (nl)	1993-04-01
NL8503597A (nl)	1986-08-01
FI72146C (fi)	1987-04-13
IE58290B1 (en)	1993-08-25
NO855308L (no)	1986-07-03
JPS61185200A (ja)	1986-08-18
DK386A (da)	1986-07-03
RO94651B (ro)	1988-07-01
NO166743B (no)	1991-05-21

Legal Events

Date	Code	Title	Description
2007-07-17	MKEX	Expiry

Publication	Publication Date	Title
CA1271705A (en)	1990-07-17	Method for the identification of nucleic acids
EP0188450B1 (de)	1992-08-12	Verfahren zum in vitro-nachweis und bestimmung unbekannter pathogene oder genetischer einheiten
EP0246864B2 (de)	2007-05-23	Hybridisierungssonden
US4562159A (en)	1985-12-31	Diagnostic test for hepatitis B virus
AU597896B2 (en)	1990-06-14	Method and kit for performing nucleic acid hybridization assays
EP0230363A2 (de)	1987-07-29	Verfahren zur Markierung von einzelsträngigen Nukleinsäuren und Hybridisierungssonden
US5981171A (en)	1999-11-09	Diagnostic assays using nucleic acid probes
Dahlén et al.	1987	Sensitive detection of genes by sandwich hybridization and time-resolved fluorometry
EP0146815B1 (de)	1990-08-16	Nukleinsäure-Hybridisierungstest mittels Antikörper gegen Einschaltungskomplexe
Stark et al.	1996	Immunomagnetic separation and solid-phase detection of Bordetella pertussis
EP0172153B1 (de)	1989-08-09	Polynucleotid-Hybridisierungsprobe
Pollice et al.	1985	Use of Non radioactive DNA Probes for the Detection of infectious Bacteria
Yasui et al.	1993	Rapid identification of Porphyromononas gingivalis by bisulfite-modified DNA probe method.
Nichols et al.	1978	A rapid purification of T4 polynucleotide kinase using Blue Dextran-Sepharose chromatography
US4831125A (en)	1989-05-16	DNA probe for corynebacterium kutscheri
EP0401313B1 (de)	1995-05-10	Makromolekulare konjugate
Stenn et al.	1988	Beyond Hybridomas Cell Identification by in Situ Nucleic Acid Hybridization
Sakamoto et al.	1989	Comparison of enzyme-linked immunosorbent assay (ELISA) with dot hybridization using 32P-or 2-acetylaminofluorene (AAF)-labelled cDNA probes for the detection and characterization of beet necrotic yellow vein virus
US5221609A (en)	1993-06-22	Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe
Harding	1981	Microbiology: Microbial Antigen Detection

CA1271705A - Method for the identification of nucleic acids - Google Patents

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