CA1299120C - COMPOSES STABLES D'ADN SERVANT A L'EXPRESSION DE L'.alpha.-1-ANTITRYPSINE - Google Patents
COMPOSES STABLES D'ADN SERVANT A L'EXPRESSION DE L'.alpha.-1-ANTITRYPSINEInfo
- Publication number
- CA1299120C CA1299120C CA000493475A CA493475A CA1299120C CA 1299120 C CA1299120 C CA 1299120C CA 000493475 A CA000493475 A CA 000493475A CA 493475 A CA493475 A CA 493475A CA 1299120 C CA1299120 C CA 1299120C
- Authority
- CA
- Canada
- Prior art keywords
- gene
- plasmid
- yeast
- dna
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 102000015395 alpha 1-Antitrypsin Human genes 0.000 title claims abstract description 10
- 108010050122 alpha 1-Antitrypsin Proteins 0.000 title claims abstract description 10
- 229940024142 alpha 1-antitrypsin Drugs 0.000 title claims abstract description 10
- 230000014509 gene expression Effects 0.000 title description 16
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 143
- 108020004414 DNA Proteins 0.000 claims abstract description 59
- 238000000034 method Methods 0.000 claims abstract description 23
- 239000003550 marker Substances 0.000 claims abstract description 18
- 230000007812 deficiency Effects 0.000 claims abstract description 16
- 230000010261 cell growth Effects 0.000 claims abstract description 7
- 230000001131 transforming effect Effects 0.000 claims abstract description 7
- 102000053602 DNA Human genes 0.000 claims abstract description 6
- 210000004027 cell Anatomy 0.000 claims description 76
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 64
- 230000006870 function Effects 0.000 claims description 27
- 230000015572 biosynthetic process Effects 0.000 claims description 20
- 244000005700 microbiome Species 0.000 claims description 14
- 101150026646 CDC4 gene Proteins 0.000 claims description 11
- 230000022131 cell cycle Effects 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 10
- 238000013518 transcription Methods 0.000 claims description 10
- 230000035897 transcription Effects 0.000 claims description 10
- 230000014616 translation Effects 0.000 claims description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 9
- 229910052799 carbon Inorganic materials 0.000 claims description 9
- 230000034659 glycolysis Effects 0.000 claims description 9
- 210000005253 yeast cell Anatomy 0.000 claims description 9
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 8
- 230000032823 cell division Effects 0.000 claims description 8
- 210000003463 organelle Anatomy 0.000 claims description 7
- 238000001243 protein synthesis Methods 0.000 claims description 7
- 230000010076 replication Effects 0.000 claims description 7
- 210000002421 cell wall Anatomy 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- 230000004543 DNA replication Effects 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 241000894007 species Species 0.000 claims description 3
- 239000003242 anti bacterial agent Substances 0.000 claims description 2
- 229940088710 antibiotic agent Drugs 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 229910001385 heavy metal Inorganic materials 0.000 claims description 2
- 101900104102 Schizosaccharomyces pombe Triosephosphate isomerase Proteins 0.000 claims 2
- 239000013612 plasmid Substances 0.000 abstract description 169
- 230000012010 growth Effects 0.000 abstract description 16
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 56
- 239000012634 fragment Substances 0.000 description 55
- 101150032817 TPI1 gene Proteins 0.000 description 43
- 101150033985 TPI gene Proteins 0.000 description 42
- 108700039887 Essential Genes Proteins 0.000 description 41
- 101001060231 Homo sapiens F-box/WD repeat-containing protein 7 Proteins 0.000 description 38
- 102100028138 F-box/WD repeat-containing protein 7 Human genes 0.000 description 37
- 230000035772 mutation Effects 0.000 description 29
- 108700015934 Triose-phosphate isomerases Proteins 0.000 description 25
- 239000013598 vector Substances 0.000 description 21
- 102000004169 proteins and genes Human genes 0.000 description 20
- 102100033598 Triosephosphate isomerase Human genes 0.000 description 17
- 101100288313 Arabidopsis thaliana KTI4 gene Proteins 0.000 description 16
- 239000002609 medium Substances 0.000 description 14
- 239000002753 trypsin inhibitor Substances 0.000 description 14
- 230000007547 defect Effects 0.000 description 12
- 238000012217 deletion Methods 0.000 description 12
- 230000037430 deletion Effects 0.000 description 12
- 230000002950 deficient Effects 0.000 description 11
- 238000010276 construction Methods 0.000 description 10
- 239000008121 dextrose Substances 0.000 description 10
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 108091026890 Coding region Proteins 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 8
- 230000006798 recombination Effects 0.000 description 8
- 230000000295 complement effect Effects 0.000 description 7
- 238000005215 recombination Methods 0.000 description 7
- 230000009466 transformation Effects 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 6
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 6
- 101150007280 LEU2 gene Proteins 0.000 description 6
- 102000005924 Triose-Phosphate Isomerase Human genes 0.000 description 6
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 6
- 210000002230 centromere Anatomy 0.000 description 6
- 238000010367 cloning Methods 0.000 description 6
- 230000029087 digestion Effects 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 5
- 101150014925 POT1 gene Proteins 0.000 description 5
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 230000036961 partial effect Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- 108020005029 5' Flanking Region Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 101000741885 Homo sapiens Protection of telomeres protein 1 Proteins 0.000 description 4
- 102100038745 Protection of telomeres protein 1 Human genes 0.000 description 4
- 229960000723 ampicillin Drugs 0.000 description 4
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000013604 expression vector Substances 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 229930182830 galactose Natural products 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 101100163849 Arabidopsis thaliana ARS1 gene Proteins 0.000 description 3
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 3
- 102000012410 DNA Ligases Human genes 0.000 description 3
- 108010061982 DNA Ligases Proteins 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 101000823116 Homo sapiens Alpha-1-antitrypsin Proteins 0.000 description 3
- 235000014676 Phragmites communis Nutrition 0.000 description 3
- 108020005091 Replication Origin Proteins 0.000 description 3
- 101100097319 Schizosaccharomyces pombe (strain 972 / ATCC 24843) ala1 gene Proteins 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 101150036080 at gene Proteins 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 108700021031 cdc Genes Proteins 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000002759 chromosomal effect Effects 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 230000002414 glycolytic effect Effects 0.000 description 3
- 102000051631 human SERPINA1 Human genes 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 229940081969 saccharomyces cerevisiae Drugs 0.000 description 3
- 239000000600 sorbitol Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 238000013519 translation Methods 0.000 description 3
- 108020005065 3' Flanking Region Proteins 0.000 description 2
- 108010023063 Bacto-peptone Proteins 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 102000004594 DNA Polymerase I Human genes 0.000 description 2
- 108010017826 DNA Polymerase I Proteins 0.000 description 2
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 101000760646 Homo sapiens Phosphatidylserine lipase ABHD16A Proteins 0.000 description 2
- 102000016387 Pancreatic elastase Human genes 0.000 description 2
- 108010067372 Pancreatic elastase Proteins 0.000 description 2
- 102100024634 Phosphatidylserine lipase ABHD16A Human genes 0.000 description 2
- 108020005115 Pyruvate Kinase Proteins 0.000 description 2
- 241000235347 Schizosaccharomyces pombe Species 0.000 description 2
- 239000004098 Tetracycline Substances 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 230000003833 cell viability Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 231100000518 lethal Toxicity 0.000 description 2
- 230000001665 lethal effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000006722 reduction reaction Methods 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 229960002180 tetracycline Drugs 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- 101150080369 tpiA gene Proteins 0.000 description 2
- 101150054879 tpiA1 gene Proteins 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- LXJXRIRHZLFYRP-VKHMYHEASA-L (R)-2-Hydroxy-3-(phosphonooxy)-propanal Natural products O=C[C@H](O)COP([O-])([O-])=O LXJXRIRHZLFYRP-VKHMYHEASA-L 0.000 description 1
- 101150072531 10 gene Proteins 0.000 description 1
- 101150098072 20 gene Proteins 0.000 description 1
- 101150110188 30 gene Proteins 0.000 description 1
- 102100030310 5,6-dihydroxyindole-2-carboxylic acid oxidase Human genes 0.000 description 1
- 101710163881 5,6-dihydroxyindole-2-carboxylic acid oxidase Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000908115 Bolivar Species 0.000 description 1
- 101150085381 CDC19 gene Proteins 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000581364 Clinitrachus argentatus Species 0.000 description 1
- 235000013175 Crataegus laevigata Nutrition 0.000 description 1
- XPYBSIWDXQFNMH-UHFFFAOYSA-N D-fructose 1,6-bisphosphate Natural products OP(=O)(O)OCC(O)C(O)C(O)C(=O)COP(O)(O)=O XPYBSIWDXQFNMH-UHFFFAOYSA-N 0.000 description 1
- LXJXRIRHZLFYRP-VKHMYHEASA-N D-glyceraldehyde 3-phosphate Chemical compound O=C[C@H](O)COP(O)(O)=O LXJXRIRHZLFYRP-VKHMYHEASA-N 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 101100457042 Dictyostelium discoideum mgst gene Proteins 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 206010014561 Emphysema Diseases 0.000 description 1
- 241001131785 Escherichia coli HB101 Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 102000005731 Glucose-6-phosphate isomerase Human genes 0.000 description 1
- 108010070600 Glucose-6-phosphate isomerase Proteins 0.000 description 1
- 101710198391 Hexokinase-1 Proteins 0.000 description 1
- 102100030338 Hexokinase-1 Human genes 0.000 description 1
- 102100029242 Hexokinase-2 Human genes 0.000 description 1
- 101710198385 Hexokinase-2 Proteins 0.000 description 1
- 108700040460 Hexokinases Proteins 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 101000801742 Homo sapiens Triosephosphate isomerase Proteins 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101100234604 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) ace-8 gene Proteins 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 101150093629 PYK1 gene Proteins 0.000 description 1
- 241001504519 Papio ursinus Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000001105 Phosphofructokinases Human genes 0.000 description 1
- 108010069341 Phosphofructokinases Proteins 0.000 description 1
- 102000012288 Phosphopyruvate Hydratase Human genes 0.000 description 1
- 108010022181 Phosphopyruvate Hydratase Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 102000013009 Pyruvate Kinase Human genes 0.000 description 1
- 230000006819 RNA synthesis Effects 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 101150006914 TRP1 gene Proteins 0.000 description 1
- 101001099217 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) Triosephosphate isomerase Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- LYMVXFSTACVOLP-ZFWWWQNUSA-N Trp-Leu Chemical compound C1=CC=C2C(C[C@H]([NH3+])C(=O)N[C@@H](CC(C)C)C([O-])=O)=CNC2=C1 LYMVXFSTACVOLP-ZFWWWQNUSA-N 0.000 description 1
- 241001672648 Vieira Species 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- RNBGYGVWRKECFJ-ZXXMMSQZSA-N alpha-D-fructofuranose 1,6-bisphosphate Chemical compound O[C@H]1[C@H](O)[C@](O)(COP(O)(O)=O)O[C@@H]1COP(O)(O)=O RNBGYGVWRKECFJ-ZXXMMSQZSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004135 animal tissue culture Methods 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- GNGACRATGGDKBX-UHFFFAOYSA-N dihydroxyacetone phosphate Chemical compound OCC(=O)COP(O)(O)=O GNGACRATGGDKBX-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- RIUKRCNLZYDWHS-UHFFFAOYSA-N ethane;methanesulfonic acid Chemical compound CC.CS(O)(=O)=O RIUKRCNLZYDWHS-UHFFFAOYSA-N 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- RNBGYGVWRKECFJ-UHFFFAOYSA-N fructose-1,6-phosphate Natural products OC1C(O)C(O)(COP(O)(O)=O)OC1COP(O)(O)=O RNBGYGVWRKECFJ-UHFFFAOYSA-N 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000009395 genetic defect Effects 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 230000008826 genomic mutation Effects 0.000 description 1
- 230000004110 gluconeogenesis Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 101150088365 intG gene Proteins 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 101150025049 leuB gene Proteins 0.000 description 1
- WQVJUBFKFCDYDQ-BBWFWOEESA-N leubethanol Natural products C1=C(C)C=C2[C@H]([C@H](CCC=C(C)C)C)CC[C@@H](C)C2=C1O WQVJUBFKFCDYDQ-BBWFWOEESA-N 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 125000001360 methionine group Chemical group N[C@@H](CCSC)C(=O)* 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 235000021048 nutrient requirements Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- KCRZDTROFIOPBP-UHFFFAOYSA-N phosphono 2,3-dihydroxypropanoate Chemical compound OCC(O)C(=O)OP(O)(O)=O KCRZDTROFIOPBP-UHFFFAOYSA-N 0.000 description 1
- 230000004260 plant-type cell wall biogenesis Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000000135 prohibitive effect Effects 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 108700022487 rRNA Genes Proteins 0.000 description 1
- 238000011536 re-plating Methods 0.000 description 1
- 230000013120 recombinational repair Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000009711 regulatory function Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 108010080629 tryptophan-leucine Proteins 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/663,315 US4931373A (en) | 1984-05-25 | 1984-10-22 | Stable DNA constructs for expression of α-1 antitrypsin |
| US663,315 | 1984-10-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1299120C true CA1299120C (fr) | 1992-04-21 |
Family
ID=24661296
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000493475A Expired - Fee Related CA1299120C (fr) | 1984-10-22 | 1985-10-21 | COMPOSES STABLES D'ADN SERVANT A L'EXPRESSION DE L'.alpha.-1-ANTITRYPSINE |
Country Status (3)
| Country | Link |
|---|---|
| JP (2) | JPH0775551B2 (fr) |
| AU (1) | AU594650B2 (fr) |
| CA (1) | CA1299120C (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60186290A (ja) * | 1983-08-10 | 1985-09-21 | チモ−ジエネテイツクス インコ−ポレ−テツド | アルフア−1−アンチトリプシンを細菌に表現させる方法 |
| FR2599752B1 (fr) * | 1986-06-10 | 1989-11-03 | Transgene Sa | Variants de l'alpha1- antitrypsine utiles notamment comme inhibiteurs de la kallikreine |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE895961A (fr) * | 1983-02-21 | 1983-06-16 | Wallone Region | Procede de preparation d'un clone bacterien produisant de 1'alpha 1-antitrypsine humaine |
| JPS60186290A (ja) * | 1983-08-10 | 1985-09-21 | チモ−ジエネテイツクス インコ−ポレ−テツド | アルフア−1−アンチトリプシンを細菌に表現させる方法 |
| US4711848A (en) * | 1984-03-14 | 1987-12-08 | Zymogenetics, Inc. | Site specific mutagenesis in alpha-1-antitrypsin |
| IL80477A0 (en) * | 1985-11-08 | 1987-02-27 | Smithkline Beckman Corp | Mutant coding sequence |
-
1985
- 1985-10-21 CA CA000493475A patent/CA1299120C/fr not_active Expired - Fee Related
- 1985-10-22 AU AU48925/85A patent/AU594650B2/en not_active Ceased
- 1985-10-22 JP JP60236262A patent/JPH0775551B2/ja not_active Expired - Fee Related
-
1992
- 1992-09-03 JP JP4277637A patent/JPH0815439B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU594650B2 (en) | 1990-03-15 |
| JPS61181397A (ja) | 1986-08-14 |
| JPH0775551B2 (ja) | 1995-08-16 |
| AU4892585A (en) | 1986-05-01 |
| JPH0815439B2 (ja) | 1996-02-21 |
| JPH0622785A (ja) | 1994-02-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4931373A (en) | Stable DNA constructs for expression of α-1 antitrypsin | |
| EP0171142B2 (fr) | Méthodes pour la production de protéines et cellules transformées et constructions d'ADN pour la correction de déficits en cellules d'hôte et leur préparation et utilisation | |
| US4959317A (en) | Site-specific recombination of DNA in eukaryotic cells | |
| EP0495208B1 (fr) | Gène de l'acide phosphatase de pichia pastoris | |
| Clark-Adams et al. | The SPT6 gene is essential for growth and is required for δ-mediated transcription in Saccharomyces cerevisiae | |
| Sikorski et al. | A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. | |
| Donahue et al. | Genetic selection for mutations that reduce or abolish ribosomal recognition of the HIS4 translational initiator region | |
| Engebrecht et al. | Yeast mer1 mutants display reduced levels of meiotic recombination. | |
| Sakai et al. | Isolation and characterization of mutants which show an oversecretion phenotype in Saccharomyces cerevisiae. | |
| US5135854A (en) | Methods of regulating protein glycosylation | |
| Russell et al. | Structure of the Schizosaccharomyces pombe cytochrome c gene | |
| US4935350A (en) | Materials and methods for controlling plasmid copy number and stability | |
| EP0314096B1 (fr) | Méthode de régulation de la glycosylation des protéines | |
| US5871957A (en) | Stable DNA constructs | |
| Koren et al. | Isolation of the positive-acting regulatory gene PHO4 from Saccharomyces cerevisiae | |
| CA1299120C (fr) | COMPOSES STABLES D'ADN SERVANT A L'EXPRESSION DE L'.alpha.-1-ANTITRYPSINE | |
| US5723292A (en) | Stable DNA constructs | |
| US5057416A (en) | Supersecreting mutants of Saccharomyces cerevisiae | |
| CA1263617A (fr) | Saccharomyces recombinant | |
| FI96121C (fi) | DNA-rakenne ja menetelmä heterologisen proteiinin tuottamiseksi | |
| EP0592358A2 (fr) | Méthode de production d'inhibiteurs de protéase | |
| KR100237979B1 (ko) | 한세눌라 폴리모르파의 지에이피디에이치 유전자 및 이의 프로모터 | |
| JPS6265694A (ja) | 超分泌性酵母変異株および該変異株からポリペプチドを産生する方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed |