CA2032035C - Fungal expression system - Google Patents

Fungal expression system Download PDF

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Publication number: CA2032035C
Authority: CA; Canada
Prior art keywords: promoter; transformed; dna; fragment; gene
Prior art date: 1990-01-29
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

CA002032035A

Other languages

English (en)

French (fr)

Other versions

CA2032035A1 (en

Inventor

Leendert Hendrik De Graaff

Henriette Catharina Van Den Broeck

Jacob Visser

Frank Buxton

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Novartis AG

Original Assignee

Novartis AG

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-01-29

Filing date

1990-12-12

Publication date

2001-08-14

1990-12-12 Application filed by Novartis AG filed Critical Novartis AG

1991-07-30 Publication of CA2032035A1 publication Critical patent/CA2032035A1/en

2001-08-14 Application granted granted Critical

2001-08-14 Publication of CA2032035C publication Critical patent/CA2032035C/en

2010-12-12 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

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229940063673 spermidine Drugs 0.000 description 1
239000000126 substance Substances 0.000 description 1
239000000758 substrate Substances 0.000 description 1
239000005720 sucrose Substances 0.000 description 1
230000009897 systematic effect Effects 0.000 description 1
235000019157 thiamine Nutrition 0.000 description 1
KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
229960003495 thiamine Drugs 0.000 description 1
239000011721 thiamine Substances 0.000 description 1
LCJVIYPJPCBWKS-NXPQJCNCSA-N thymosin Chemical compound SC[C@@H](N)C(=O)N[C@H](CO)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CO)C(=O)N[C@H](CO)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H]([C@@H](C)CC)C(=O)N[C@H]([C@H](C)O)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CCCCN)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](C(C)C)C(=O)N[C@H](C(C)C)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@H](CCC(O)=O)C(O)=O LCJVIYPJPCBWKS-NXPQJCNCSA-N 0.000 description 1
230000002103 transcriptional effect Effects 0.000 description 1
238000012546 transfer Methods 0.000 description 1
238000013519 translation Methods 0.000 description 1
108700004896 tripeptide FEG Proteins 0.000 description 1
239000001226 triphosphate Substances 0.000 description 1
235000011178 triphosphate Nutrition 0.000 description 1
UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
101150016309 trpC gene Proteins 0.000 description 1
239000002753 trypsin inhibitor Substances 0.000 description 1
108010084932 tryptophyl-proline Proteins 0.000 description 1
230000007306 turnover Effects 0.000 description 1
108010020532 tyrosyl-proline Proteins 0.000 description 1
108010078580 tyrosylleucine Proteins 0.000 description 1
238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
241001515965 unidentified phage Species 0.000 description 1
229960005356 urokinase Drugs 0.000 description 1
229960005486 vaccine Drugs 0.000 description 1
IBIDRSSEHFLGSD-UHFFFAOYSA-N valinyl-arginine Natural products CC(C)C(N)C(=O)NC(C(O)=O)CCCN=C(N)N IBIDRSSEHFLGSD-UHFFFAOYSA-N 0.000 description 1
108010073969 valyllysine Proteins 0.000 description 1
108700026220 vif Genes Proteins 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01015—Polygalacturonase (3.2.1.15)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
General Health & Medical Sciences (AREA)
General Engineering & Computer Science (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Mycology (AREA)
Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Agricultural Chemicals And Associated Chemicals (AREA)
Mushroom Cultivation (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Compounds Of Unknown Constitution (AREA)
Saccharide Compounds (AREA)
Preparation Of Fruits And Vegetables (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Selective Calling Equipment (AREA)
Analysing Materials By The Use Of Radiation (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Circuits Of Receivers In General (AREA)
Optical Communication System (AREA)
Peptides Or Proteins (AREA)
Confectionery (AREA)

CA002032035A 1990-01-29 1990-12-12 Fungal expression system Expired - Lifetime CA2032035C (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP90810068		1990-01-29
EP90810068.8		1990-01-29

Publications (2)

Publication Number	Publication Date
CA2032035A1 CA2032035A1 (en)	1991-07-30
CA2032035C true CA2032035C (en)	2001-08-14

Family

ID=8205903

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002032035A Expired - Lifetime CA2032035C (en)	1990-01-29	1990-12-12	Fungal expression system

Country Status (18)

Country	Link
EP (1)	EP0439997B1 (de)
JP (1)	JP3329472B2 (de)
KR (1)	KR0157411B1 (de)
AT (1)	ATE130034T1 (de)
AU (1)	AU645768B2 (de)
CA (1)	CA2032035C (de)
DE (1)	DE69023474T2 (de)
DK (1)	DK0439997T3 (de)
ES (1)	ES2079468T3 (de)
FI (1)	FI103206B (de)
GR (1)	GR3018044T3 (de)
HU (1)	HU210989B (de)
IE (1)	IE68457B1 (de)
MX (1)	MX23984A (de)
NO (1)	NO309283B1 (de)
NZ (1)	NZ236434A (de)
PT (1)	PT96310B (de)
ZA (1)	ZA909987B (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
BE1005432A4 (nl) *	1991-10-01	1993-07-20	Dsm Nv	Expressiecassette voor heterologe eiwitten.
JP2866739B2 (ja) *	1991-12-09	1999-03-08	ユニリーバー・ナームローゼ・ベンノートシヤープ	アスペルギルスエンドキシラナーゼ▲ｉｉ▼遺伝子に由来する発現／分泌調節領域を用い、形質転換したカビでタンパク質を産生して分泌させるための方法
EP0688359A1 (de) *	1993-02-16	1995-12-27	Novo Nordisk A/S	Ein enzym mit pectinlyaseaktivität
US5674728A (en) *	1993-11-03	1997-10-07	Novartis Corporation	Aspergillus niger vacuolar aspartyl protease
ATE207538T1 (de)	1995-02-09	2001-11-15	Behringwerke Ag	Verfahren zur produktion von proteinen
EP1355939A2 (de)	2000-11-03	2003-10-29	Pestka Biomedical Laboratories, Inc.	Interferone, deren verwendungen und zusammensetzungen
DE10356218A1 (de) *	2003-12-03	2005-06-30	Bayer Cropscience Ag	Verfahren zum Identifizieren von fungizid wirksamen Verbindungen basierend auf Pyruvatkinasen aus Pilzen
EP1861421B1 (de) *	2005-03-16	2011-10-19	Novozymes Adenium Biotech A/S	Rekombinante expression von insekten defensinen in aspergillus

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA1341302C (en) *	1983-02-22	2001-10-09	Rae Lyn Burke	Yeast expression systems with vectors having gapdh or pyk promoters and synthesis of foreign protein
US4885249A (en) *	1984-12-05	1989-12-05	Allelix, Inc.	Aspergillus niger transformation system
FI864473A7 (fi) *	1985-11-20	1987-05-21	Panlabs Inc	Yhdistetyt ilmaisukasetit fungaalitransformaatiota varten.

1990
- 1990-12-12 ZA ZA909987A patent/ZA909987B/xx unknown
- 1990-12-12 IE IE448190A patent/IE68457B1/en not_active IP Right Cessation
- 1990-12-12 FI FI906123A patent/FI103206B/fi active IP Right Grant
- 1990-12-12 CA CA002032035A patent/CA2032035C/en not_active Expired - Lifetime
- 1990-12-12 NZ NZ236434A patent/NZ236434A/en unknown
- 1990-12-13 NO NO905393A patent/NO309283B1/no not_active IP Right Cessation
- 1990-12-18 HU HU908325A patent/HU210989B/hu unknown
- 1990-12-20 ES ES90811008T patent/ES2079468T3/es not_active Expired - Lifetime
- 1990-12-20 AT AT90811008T patent/ATE130034T1/de not_active IP Right Cessation
- 1990-12-20 DK DK90811008.3T patent/DK0439997T3/da active
- 1990-12-20 EP EP90811008A patent/EP0439997B1/de not_active Expired - Lifetime
- 1990-12-20 DE DE69023474T patent/DE69023474T2/de not_active Expired - Lifetime
- 1990-12-21 PT PT96310A patent/PT96310B/pt not_active IP Right Cessation
- 1990-12-24 AU AU68460/90A patent/AU645768B2/en not_active Expired
- 1990-12-26 KR KR1019900021723A patent/KR0157411B1/ko not_active Expired - Lifetime
- 1990-12-28 JP JP41858290A patent/JP3329472B2/ja not_active Expired - Lifetime
- 1990-12-28 MX MX2398490A patent/MX23984A/es unknown
1995
- 1995-11-09 GR GR950403015T patent/GR3018044T3/el unknown

Also Published As

Publication number	Publication date
PT96310A (pt)	1991-10-15
KR910014507A (ko)	1991-08-31
NO905393D0 (no)	1990-12-13
NO309283B1 (no)	2001-01-08
HUT56886A (en)	1991-10-28
EP0439997A1 (de)	1991-08-07
FI906123L (fi)	1991-07-30
IE904481A1 (en)	1991-07-31
ES2079468T3 (es)	1996-01-16
NO905393L (no)	1991-07-30
ATE130034T1 (de)	1995-11-15
DK0439997T3 (da)	1995-12-11
CA2032035A1 (en)	1991-07-30
DE69023474D1 (de)	1995-12-14
IE68457B1 (en)	1996-06-26
AU6846090A (en)	1991-08-01
JPH05192150A (ja)	1993-08-03
NZ236434A (en)	1992-08-26
MX23984A (es)	1994-02-28
PT96310B (pt)	1998-06-30
FI906123A0 (fi)	1990-12-12
KR0157411B1 (ko)	1998-10-15
JP3329472B2 (ja)	2002-09-30
DE69023474T2 (de)	1996-03-28
AU645768B2 (en)	1994-01-27
FI103206B1 (fi)	1999-05-14
FI103206B (fi)	1999-05-14
ZA909987B (en)	1991-09-25
HU908325D0 (en)	1991-07-29
GR3018044T3 (en)	1996-02-29
HU210989B (en)	1995-09-28
EP0439997B1 (de)	1995-11-08

Legal Events

Date	Code	Title	Description
1997-10-30	EEER	Examination request
2010-12-12	MKEX	Expiry

Publication	Publication Date	Title
JP2633885B2 (ja)	1997-07-23	新規な発現系
JP3424840B2 (ja)	2003-07-07	菌類プロテアーゼ
CA1338859C (en)	1997-01-21	Expression system
EP0439997B1 (de)	1995-11-08	Expressionssysteme in Pilzen
CA2024487C (en)	2008-04-29	Polygalacturonase encoding fungal expression system
US5447862A (en)	1995-09-05	Pectin lyase genes of aspergillus niger
CA1335081C (en)	1995-04-04	Expression system comprising aspergillus niger pectin lyase i gene sequences
DD296699A5 (de)	1991-12-12	Neuartiges expressionssystem