CA2043631C - Detecting and imaging in biochemical assays using phosphor screens - Google Patents

Detecting and imaging in biochemical assays using phosphor screens

Info

Publication number: CA2043631C
Authority: CA; Canada
Prior art keywords: macromolecules; chemiluminescent; liquid; matrix; substrate
Prior art date: 1990-07-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

CA002043631A

Other languages

English (en)

French (fr)

Other versions

CA2043631A1 (en

Inventor

George Bers

Franklin R. Witney

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Bio Rad Laboratories Inc

Original Assignee

Bio Rad Laboratories Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-07-12

Filing date

1991-05-31

Publication date

1998-04-21

1991-05-31 Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc

1992-01-13 Publication of CA2043631A1 publication Critical patent/CA2043631A1/en

1998-04-21 Application granted granted Critical

1998-04-21 Publication of CA2043631C publication Critical patent/CA2043631C/en

2011-05-31 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title claims abstract description 32
238000003384 imaging method Methods 0.000 title description 6
238000010256 biochemical assay Methods 0.000 title description 3
239000000758 substrate Substances 0.000 claims abstract description 56
229920002521 macromolecule Polymers 0.000 claims abstract description 36
238000006243 chemical reaction Methods 0.000 claims abstract description 29
239000007791 liquid phase Substances 0.000 claims abstract description 19
238000000034 method Methods 0.000 claims description 31
239000011159 matrix material Substances 0.000 claims description 19
230000003993 interaction Effects 0.000 claims description 10
239000012528 membrane Substances 0.000 claims description 7
230000027455 binding Effects 0.000 claims description 6
239000000203 mixture Substances 0.000 claims description 6
150000001875 compounds Chemical class 0.000 claims description 5
102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims description 4
108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims description 4
230000001939 inductive effect Effects 0.000 claims description 4
230000005855 radiation Effects 0.000 claims description 4
239000007787 solid Substances 0.000 claims description 4
239000007864 aqueous solution Substances 0.000 claims description 2
230000004888 barrier function Effects 0.000 claims description 2
230000005670 electromagnetic radiation Effects 0.000 claims description 2
230000004936 stimulating effect Effects 0.000 claims description 2
102000045595 Phosphoprotein Phosphatases Human genes 0.000 claims 1
108700019535 Phosphoprotein Phosphatases Proteins 0.000 claims 1
238000006911 enzymatic reaction Methods 0.000 claims 1
230000000717 retained effect Effects 0.000 claims 1
108090000623 proteins and genes Proteins 0.000 abstract description 3
150000007523 nucleic acids Chemical group 0.000 abstract description 2
102000004169 proteins and genes Human genes 0.000 abstract 1
239000000463 material Substances 0.000 description 11
102000004190 Enzymes Human genes 0.000 description 9
108090000790 Enzymes Proteins 0.000 description 9
230000002285 radioactive effect Effects 0.000 description 9
238000001514 detection method Methods 0.000 description 7
239000004033 plastic Substances 0.000 description 7
229920003023 plastic Polymers 0.000 description 7
102000002260 Alkaline Phosphatase Human genes 0.000 description 6
108020004774 Alkaline Phosphatase Proteins 0.000 description 6
238000000354 decomposition reaction Methods 0.000 description 6
239000000243 solution Substances 0.000 description 6
239000003054 catalyst Substances 0.000 description 5
230000035945 sensitivity Effects 0.000 description 5
239000007790 solid phase Substances 0.000 description 5
108020004414 DNA Proteins 0.000 description 4
TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 description 4
230000005281 excited state Effects 0.000 description 4
238000002372 labelling Methods 0.000 description 4
239000007788 liquid Substances 0.000 description 4
PQXKHYXIUOZZFA-UHFFFAOYSA-M lithium fluoride Chemical compound [Li+].[F-] PQXKHYXIUOZZFA-UHFFFAOYSA-M 0.000 description 4
BVTJGGGYKAMDBN-UHFFFAOYSA-N Dioxetane Chemical compound C1COO1 BVTJGGGYKAMDBN-UHFFFAOYSA-N 0.000 description 3
WAEMQWOKJMHJLA-UHFFFAOYSA-N Manganese(2+) Chemical compound [Mn+2] WAEMQWOKJMHJLA-UHFFFAOYSA-N 0.000 description 3
238000003556 assay Methods 0.000 description 3
238000010790 dilution Methods 0.000 description 3
239000012895 dilution Substances 0.000 description 3
238000002474 experimental method Methods 0.000 description 3
HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 3
239000000376 reactant Substances 0.000 description 3
230000009870 specific binding Effects 0.000 description 3
230000000638 stimulation Effects 0.000 description 3
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
UMTZYGZMIJRCFG-UHFFFAOYSA-N 3-phenyldioxetane Chemical compound C1OOC1C1=CC=CC=C1 UMTZYGZMIJRCFG-UHFFFAOYSA-N 0.000 description 2
238000001712 DNA sequencing Methods 0.000 description 2
239000004677 Nylon Substances 0.000 description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
OAICVXFJPJFONN-OUBTZVSYSA-N Phosphorus-32 Chemical compound [32P] OAICVXFJPJFONN-OUBTZVSYSA-N 0.000 description 2
-1 alkaline earth metal sulfides Chemical class 0.000 description 2
238000003491 array Methods 0.000 description 2
229960002685 biotin Drugs 0.000 description 2
239000011616 biotin Substances 0.000 description 2
229910000420 cerium oxide Inorganic materials 0.000 description 2
238000011161 development Methods 0.000 description 2
230000004907 flux Effects 0.000 description 2
239000000499 gel Substances 0.000 description 2
230000005283 ground state Effects 0.000 description 2
238000009396 hybridization Methods 0.000 description 2
230000002209 hydrophobic effect Effects 0.000 description 2
238000013507 mapping Methods 0.000 description 2
229910052751 metal Inorganic materials 0.000 description 2
239000002184 metal Substances 0.000 description 2
229920001778 nylon Polymers 0.000 description 2
230000003287 optical effect Effects 0.000 description 2
BMMGVYCKOGBVEV-UHFFFAOYSA-N oxo(oxoceriooxy)cerium Chemical compound [Ce]=O.O=[Ce]=O BMMGVYCKOGBVEV-UHFFFAOYSA-N 0.000 description 2
229940097886 phosphorus 32 Drugs 0.000 description 2
230000008569 process Effects 0.000 description 2
238000002731 protein assay Methods 0.000 description 2
KZUNJOHGWZRPMI-UHFFFAOYSA-N samarium atom Chemical compound [Sm] KZUNJOHGWZRPMI-UHFFFAOYSA-N 0.000 description 2
239000000523 sample Substances 0.000 description 2
239000000126 substance Substances 0.000 description 2
WYNZXNXFHYJUTE-UHFFFAOYSA-N 1,2-dioxetanedione Chemical class O=C1OOC1=O WYNZXNXFHYJUTE-UHFFFAOYSA-N 0.000 description 1
ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 1
ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 1
CDEMHJCJMMOFMB-UHFFFAOYSA-M ClC1=CC=C([Mg]Br)C=C1 Chemical compound ClC1=CC=C([Mg]Br)C=C1 CDEMHJCJMMOFMB-UHFFFAOYSA-M 0.000 description 1
239000004971 Cross linker Substances 0.000 description 1
102000053602 DNA Human genes 0.000 description 1
239000003298 DNA probe Substances 0.000 description 1
KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 description 1
102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
108060001084 Luciferase Proteins 0.000 description 1
239000005089 Luciferase Substances 0.000 description 1
239000000020 Nitrocellulose Substances 0.000 description 1
108020004518 RNA Probes Proteins 0.000 description 1
239000003391 RNA probe Substances 0.000 description 1
229910052772 Samarium Inorganic materials 0.000 description 1
UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
239000011543 agarose gel Substances 0.000 description 1
229910052784 alkaline earth metal Inorganic materials 0.000 description 1
PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
230000003321 amplification Effects 0.000 description 1
230000009830 antibody antigen interaction Effects 0.000 description 1
230000009286 beneficial effect Effects 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
235000020958 biotin Nutrition 0.000 description 1
230000001276 controlling effect Effects 0.000 description 1
238000010586 diagram Methods 0.000 description 1
MKQRDVRSSVPGEB-UHFFFAOYSA-N dioxetan-3-one Chemical group O=C1COO1 MKQRDVRSSVPGEB-UHFFFAOYSA-N 0.000 description 1
239000002019 doping agent Substances 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
230000002708 enhancing effect Effects 0.000 description 1
125000004185 ester group Chemical group 0.000 description 1
OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 description 1
229910001940 europium oxide Inorganic materials 0.000 description 1
230000007717 exclusion Effects 0.000 description 1
239000011521 glass Substances 0.000 description 1
229910052500 inorganic mineral Inorganic materials 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
150000002739 metals Chemical class 0.000 description 1
239000011707 mineral Substances 0.000 description 1
229920001220 nitrocellulos Polymers 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
125000002524 organometallic group Chemical group 0.000 description 1
239000002245 particle Substances 0.000 description 1
150000002978 peroxides Chemical class 0.000 description 1
239000012071 phase Substances 0.000 description 1
235000011007 phosphoric acid Nutrition 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
230000005258 radioactive decay Effects 0.000 description 1
239000012857 radioactive material Substances 0.000 description 1
229910052761 rare earth metal Inorganic materials 0.000 description 1
150000002910 rare earth metals Chemical class 0.000 description 1
230000001105 regulatory effect Effects 0.000 description 1
230000004044 response Effects 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
229910001954 samarium oxide Inorganic materials 0.000 description 1
238000012216 screening Methods 0.000 description 1
150000003346 selenoethers Chemical class 0.000 description 1
ZEGFMFQPWDMMEP-UHFFFAOYSA-N strontium;sulfide Chemical compound [S-2].[Sr+2] ZEGFMFQPWDMMEP-UHFFFAOYSA-N 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
238000013519 translation Methods 0.000 description 1
230000000007 visual effect Effects 0.000 description 1
229910052844 willemite Inorganic materials 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Immunology (AREA)
Physics & Mathematics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
General Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Analytical Chemistry (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Biophysics (AREA)
Genetics & Genomics (AREA)
Urology & Nephrology (AREA)
Hematology (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Biomedical Technology (AREA)
Cell Biology (AREA)
Plasma & Fusion (AREA)
Chemical Kinetics & Catalysis (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Investigating Or Analysing Biological Materials (AREA)

CA002043631A 1990-07-12 1991-05-31 Detecting and imaging in biochemical assays using phosphor screens Expired - Fee Related CA2043631C (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US55196190A	1990-07-12	1990-07-12
US07/551,961		1990-07-12

Publications (2)

Publication Number	Publication Date
CA2043631A1 CA2043631A1 (en)	1992-01-13
CA2043631C true CA2043631C (en)	1998-04-21

Family

ID=24203384

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA002043631A Expired - Fee Related CA2043631C (en)	1990-07-12	1991-05-31	Detecting and imaging in biochemical assays using phosphor screens

Country Status (7)

Country	Link
JP (1)	JPH04232864A (it)
CA (1)	CA2043631C (it)
DE (1)	DE4122839A1 (it)
FR (1)	FR2664703B1 (it)
GB (1)	GB2246197B (it)
HK (1)	HK40795A (it)
IT (1)	ITRM910503A1 (it)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA2130947C (en) *	1993-11-12	2001-02-06	Robert E. Emmons	Dry elements, test devices, test kits and methods for chemiluminescent detection of analytes using peroxidase-labeled reagents
DE4410655C2 (de) *	1994-03-26	2001-06-28	Heiko Schwertner	Verfahren und Vorrichtung zur schnellen kontinuierlichen Sequenzanalyse von verschiedenen linearen polymeren Substanzen nach einer einheitlichen Vorgehensweise
US6415038B1 (en)	1994-05-20	2002-07-02	Fuji Photo Film Co., Ltd.	Image analyzing apparatus
JP3571819B2 (ja) *	1995-01-09	2004-09-29	富士写真フイルム株式会社	生化学画像解析装置
JP3550203B2 (ja) *	1995-01-10	2004-08-04	富士写真フイルム株式会社	画像解析装置
US5672514A (en) *	1995-02-01	1997-09-30	Fuji Photo Film Co., Ltd.	Chemiluminescent detecting method and apparatus
JPH1021373A (ja) *	1996-07-05	1998-01-23	Fuji Photo Film Co Ltd	画像解析装置
JP3851699B2 (ja)	1997-02-06	2006-11-29	富士写真フイルム株式会社	画像解析装置
DE19704731B4 (de) *	1997-02-07	2006-07-27	Stratec Biomedical Systems Ag	Meßgerät zur Durchführung von Lumineszenzmessungen
RU2132061C1 (ru) *	1997-02-19	1999-06-20	ЗАО "Медицинские компьютерные системы"	Способ адаптивной автоматической сегментации и распознавания клеток на изображениях цитологических препаратов
JP3474765B2 (ja)	1998-03-17	2003-12-08	富士写真フイルム株式会社	画像記録・読み取りシステム
US6572095B1 (en)	1999-09-03	2003-06-03	Fuji Photo Film Co., Ltd.	Method of and system for conveying sheet to be scanned
JP3897272B2 (ja)	1999-09-28	2007-03-22	富士フイルム株式会社	画像解析装置
US6781143B2 (en) *	2001-04-06	2004-08-24	Fuji Photo Film Co., Ltd.	Biochemical analysis data producing method, biochemical analysis data producing apparatus and stimulable phosphor sheet used therefor
JP3940995B2 (ja)	2002-04-19	2007-07-04	富士フイルム株式会社	画像読取装置
JP3913602B2 (ja)	2002-04-23	2007-05-09	富士フイルム株式会社	読取画像処理方法および装置

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4396579A (en) *	1981-08-06	1983-08-02	Miles Laboratories, Inc.	Luminescence detection device
GB2116709B (en) *	1982-03-09	1986-02-19	Wira & Mather	Microbiological testing detecting surface mildew
AU7751687A (en) *	1986-07-22	1988-02-10	Boyce Thompson Institute For Plant Research Inc.	Use of bacterial luciferase structural genes for cloning and monitoring gene expression in microorganisms and for tagging and identification of genetically engineered organisms
CA1250212A (en) *	1987-04-23	1989-02-21	Merlin M.L. Leong	Method and apparatus for instant photodetection of chemiluminescent immunoassays
US4797553A (en) *	1987-05-11	1989-01-10	E. I. Dupont De Nemours And Company	Method and apparatus for reading a transparent photostimulable luminescent screen
GB2207245A (en) *	1987-07-15	1989-01-25	Unilever Plc	Specific binding chemiluminescent assay
JPH01219563A (ja) *	1988-02-27	1989-09-01	Doujin Kagaku Kenkyusho:Kk	化学発光の写真法による検出方法
GB2233450B (en) *	1989-06-24	1993-06-30	Univ Wales Medicine	Detecting or quantifing multiple analytes with luminescent reagents

1991
- 1991-05-17 JP JP3112891A patent/JPH04232864A/ja active Pending
- 1991-05-31 CA CA002043631A patent/CA2043631C/en not_active Expired - Fee Related
- 1991-07-04 FR FR9108381A patent/FR2664703B1/fr not_active Expired - Fee Related
- 1991-07-10 IT IT91RM000503A patent/ITRM910503A1/it not_active IP Right Cessation
- 1991-07-10 DE DE4122839A patent/DE4122839A1/de not_active Ceased
- 1991-07-12 GB GB9115090A patent/GB2246197B/en not_active Expired - Fee Related
1995
- 1995-03-23 HK HK40795A patent/HK40795A/xx not_active IP Right Cessation

Also Published As

Publication number	Publication date
CA2043631A1 (en)	1992-01-13
HK40795A (en)	1995-03-31
FR2664703B1 (fr)	1995-05-12
GB2246197A (en)	1992-01-22
ITRM910503A1 (it)	1992-01-13
ITRM910503A0 (it)	1991-07-10
DE4122839A1 (de)	1992-01-16
FR2664703A1 (fr)	1992-01-17
GB9115090D0 (en)	1991-08-28
JPH04232864A (ja)	1992-08-21
GB2246197B (en)	1994-03-16

Legal Events

Date	Code	Title	Description
1992-07-06	EEER	Examination request
1999-05-31	MKLA	Lapsed

Publication	Publication Date	Title
CA2043631C (en)	1998-04-21	Detecting and imaging in biochemical assays using phosphor screens
JP2634783B2 (ja)	1997-07-30	蛍光体イメージングスクリーン及び輻射線像の記録再生方法
US7170078B2 (en)	2007-01-30	Biochemical analysis data producing method and scanner used therefor
US5270162A (en)	1993-12-14	Autoradiographic gene-screening method
US7348587B2 (en)	2008-03-25	Method for producing biochemical analysis data and apparatus used therefor
US6617590B2 (en)	2003-09-09	Image reading apparatus
JP3719932B2 (ja)	2005-11-24	シンチレーションプロキシミティー試験
US5672514A (en)	1997-09-30	Chemiluminescent detecting method and apparatus
US6835946B2 (en)	2004-12-28	Image reading method and apparatus
EP0113673A2 (en)	1984-07-18	Signal processing method in autoradiography
EP1186886A2 (en)	2002-03-13	Biochemical analyzing method and biochemical analysis apparatus used therefor
US20030003594A1 (en)	2003-01-02	Method for producing biochemical analysis data and scanner used therefor
CA1257184A (en)	1989-07-11	Method for determining base sequence of dna or dna fragment utilizing autoradiography
CA1228002A (en)	1987-10-13	Autoradiographic gene-screening method
US6781143B2 (en)	2004-08-24	Biochemical analysis data producing method, biochemical analysis data producing apparatus and stimulable phosphor sheet used therefor
US20020150944A1 (en)	2002-10-17	Biochemical analysis unit and method for exposing stimulable phosphor sheet using the same
US20030003600A1 (en)	2003-01-02	Biochemical analysis kit and method for exposing stimulable phosphor sheet
JP2646814B2 (ja)	1997-08-27	核酸等の検定方法
US20020195573A1 (en)	2002-12-26	Stimulable phosphor sheet and method for manufacturing the same
US20030003493A1 (en)	2003-01-02	Biochemical analysis unit and biochemical analysis kit
US7189577B2 (en)	2007-03-13	Biochemical analysis kit and method for exposing stimulable phosphor sheet
JPH04148699A (ja)	1992-05-21	生体成分測定用担体
JP2003028863A (ja)	2003-01-29	生化学解析システム
Grosvenor	1999	Development of a method for performing binding assays in picoliter volumes
JPH08247949A (ja)	1996-09-27	化学発光検出方法および装置