CA2046919A1 - Paires d'amorces oligonucleotidiques pour l'amplification d'un gene de sequence inconnue - Google Patents

Paires d'amorces oligonucleotidiques pour l'amplification d'un gene de sequence inconnue

Info

Publication number
CA2046919A1
CA2046919A1 CA 2046919 CA2046919A CA2046919A1 CA 2046919 A1 CA2046919 A1 CA 2046919A1 CA 2046919 CA2046919 CA 2046919 CA 2046919 A CA2046919 A CA 2046919A CA 2046919 A1 CA2046919 A1 CA 2046919A1
Authority
CA
Canada
Prior art keywords
primer pair
dna
target dna
oligonucleotide primer
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2046919
Other languages
English (en)
Inventor
Mark P. Pasek
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biogen Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2046919A1 publication Critical patent/CA2046919A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CA 2046919 1989-02-14 1990-02-14 Paires d'amorces oligonucleotidiques pour l'amplification d'un gene de sequence inconnue Abandoned CA2046919A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31076389A 1989-02-14 1989-02-14
US310,763 1989-02-14

Publications (1)

Publication Number Publication Date
CA2046919A1 true CA2046919A1 (fr) 1990-08-15

Family

ID=23203992

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2046919 Abandoned CA2046919A1 (fr) 1989-02-14 1990-02-14 Paires d'amorces oligonucleotidiques pour l'amplification d'un gene de sequence inconnue

Country Status (4)

Country Link
EP (1) EP0458909A1 (fr)
AU (1) AU5404890A (fr)
CA (1) CA2046919A1 (fr)
WO (1) WO1990009457A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE144556T1 (de) * 1988-06-24 1996-11-15 Amgen Inc Verfahren und mittel zum nachweis von nukleinsäuresequenzen
GB8818020D0 (en) * 1988-07-28 1988-09-01 Ici Plc Method for amplification of nucleotide sequences
EP0545459A1 (fr) * 1989-01-31 1993-06-09 The Regents Of The University Of California Amplification de l'ADN chromosomique trié par cytométrie
EP0456721A4 (en) * 1989-01-31 1992-06-03 University Of Miami Microdissection and amplification of chromosomal dna
WO1992002642A1 (fr) * 1990-08-10 1992-02-20 Chiron Corporation Diagnostics du virus non a, non b: polynucleotides utiles dans la detection du virus de l'hepatite c
FR2678639B1 (fr) * 1991-07-03 1993-09-17 Rhone Poulenc Rorer Sa Procede de clonage d'acides nucleiques.
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
EP1047794A2 (fr) * 1998-01-13 2000-11-02 BioChip Technologies GmbH Procede de detection de sequences nucleotidiques
DE10108626A1 (de) * 2001-02-22 2002-09-05 Sahin Ugur Verfahren zur Identifizierung biologisch aktiver Strukturen mikrobieller Erreger

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
US6107023A (en) * 1988-06-17 2000-08-22 Genelabs Technologies, Inc. DNA amplification and subtraction techniques
EP0426756A4 (en) * 1988-07-26 1991-11-21 Genelabs Incorporated Rna and dna amplification techniques
AU3974989A (en) * 1988-07-26 1990-02-19 Genelabs Incorporated Sequence-specific amplification techniques

Also Published As

Publication number Publication date
AU5404890A (en) 1990-09-05
WO1990009457A2 (fr) 1990-08-23
EP0458909A1 (fr) 1991-12-04
WO1990009457A3 (fr) 1990-10-04

Similar Documents

Publication Publication Date Title
US6107023A (en) DNA amplification and subtraction techniques
US5366877A (en) Restriction/ligation labeling for primer initiated multiple copying of DNA ssequences
US5830643A (en) Partially double-stranded oligonucleotide and method for forming oligonucleotide
AU622426B2 (en) Assay using template-dependent nucleic acid probe reorganization
EP0593095B1 (fr) Clonage genomique et cartographie
JP2954232B2 (ja) 核酸配列の増幅および検出法
US5830711A (en) Thermostable ligase mediated DNA amplification system for the detection of genetic diseases
JP3514630B2 (ja) 核酸配列の増幅および検出
JPH025864A (ja) 核酸増幅方法
JPH08511684A (ja) 熱安定性ｄｎａポリメラーゼ由来の５’ヌクレアーゼ
JPH07500734A (ja) オリゴヌクレオチドプローブのポリマーを用いる核酸配列の検出方法
JP2003535587A (ja) 低温感受性変異体ｄｎａポリメラーゼ
WO1991017239A1 (fr) Systeme d'amplification d'adn induit par ligase thermostable, servant a detecter des maladies genetiques.
PT92404A (pt) Processo para a caracterizacao de adn genomico e para a preparacao de polinucleotidos uteis para essa caracterizacao
CA2046919A1 (fr) Paires d'amorces oligonucleotidiques pour l'amplification d'un gene de sequence inconnue
CN114410738A (zh) 制备在利用核酸聚合酶来检测核酸时使用的高精度核酸的方法
TWI316964B (fr)
US20020102660A1 (en) Method for synthesis of nucleic acids
JPH05508305A (ja) 非特異的ｄｎａ増幅
JP3494509B2 (ja) 核酸合成法
JP2928992B2 (ja) Ｄｎａ及び／又はｒｎａを特異的に増幅し検出する方法
CA2395920A1 (fr) Procede servant a generer des fragments d'adnc plus longs a partir d'etiquettes de sage afin d'identifier des genes
JPH09187277A (ja) 鋳型として全血液を用いた核酸の直接的ｐｃｒ増幅法
JPH03119999A (ja) 核酸を増幅して検出するための方法及び診断試験キット
WO1998002574A1 (fr) Procede pour l'examen d'acides nucleiques et kits d'examen

Legal Events

Date Code Title Description
FZDE Dead