CA2111984A1 - Additif d'un milieu de culture contenant un agent chelateur du fer - Google Patents

Additif d'un milieu de culture contenant un agent chelateur du fer

Info

Publication number: CA2111984A1
Authority: CA; Canada
Prior art keywords: citrate; iron; serum; culture medium; free
Prior art date: 1991-06-21
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Abandoned

Application number

CA 2111984

Other languages

English (en)

Inventor

Peter B. Suhr-Jessen

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Novo Nordisk AS

Original Assignee

Individual

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-06-21

Filing date

1992-06-18

Publication date

1993-01-07

1992-06-18 Application filed by Individual filed Critical Individual

1993-01-07 Publication of CA2111984A1 publication Critical patent/CA2111984A1/fr

Status Abandoned legal-status Critical Current

Links

XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 title claims abstract description 49
229910052742 iron Inorganic materials 0.000 title claims abstract description 23
239000000654 additive Substances 0.000 title claims abstract description 20
230000000996 additive effect Effects 0.000 title claims abstract description 20
239000001963 growth medium Substances 0.000 title claims abstract description 19
239000013522 chelant Substances 0.000 title claims abstract description 10
150000002505 iron Chemical class 0.000 claims abstract description 15
229910052783 alkali metal Inorganic materials 0.000 claims abstract description 13
150000001340 alkali metals Chemical class 0.000 claims abstract description 13
229910052784 alkaline earth metal Inorganic materials 0.000 claims abstract description 13
-1 alkaline earth metal citrate Chemical class 0.000 claims abstract description 13
239000002609 medium Substances 0.000 claims description 22
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims description 17
229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 12
RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 12
NMCUIPGRVMDVDB-UHFFFAOYSA-L iron dichloride Chemical compound Cl[Fe]Cl NMCUIPGRVMDVDB-UHFFFAOYSA-L 0.000 claims description 10
210000004962 mammalian cell Anatomy 0.000 claims description 10
229910021577 Iron(II) chloride Inorganic materials 0.000 claims description 9
229910021579 Iron(II) iodide Inorganic materials 0.000 claims description 3
BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 3
229910000155 iron(II) phosphate Inorganic materials 0.000 claims description 3
229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 3
VCJMYUPGQJHHFU-UHFFFAOYSA-N iron(III) nitrate Inorganic materials [Fe+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O VCJMYUPGQJHHFU-UHFFFAOYSA-N 0.000 claims description 3
BQZGVMWPHXIKEQ-UHFFFAOYSA-L iron(ii) iodide Chemical compound [Fe+2].[I-].[I-] BQZGVMWPHXIKEQ-UHFFFAOYSA-L 0.000 claims description 3
QEEAPRPFLLJWCF-UHFFFAOYSA-K potassium citrate (anhydrous) Chemical compound [K+].[K+].[K+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QEEAPRPFLLJWCF-UHFFFAOYSA-K 0.000 claims description 3
210000004027 cell Anatomy 0.000 description 23
235000015097 nutrients Nutrition 0.000 description 10
102000004338 Transferrin Human genes 0.000 description 9
108090000901 Transferrin Proteins 0.000 description 9
239000012581 transferrin Substances 0.000 description 9
238000002474 experimental method Methods 0.000 description 8
239000012679 serum free medium Substances 0.000 description 8
FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 description 7
239000004017 serum-free culture medium Substances 0.000 description 7
239000011550 stock solution Substances 0.000 description 7
102000004169 proteins and genes Human genes 0.000 description 5
108090000623 proteins and genes Proteins 0.000 description 5
238000004113 cell culture Methods 0.000 description 4
210000004408 hybridoma Anatomy 0.000 description 4
238000013337 sub-cultivation Methods 0.000 description 4
239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 3
210000004978 chinese hamster ovary cell Anatomy 0.000 description 3
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
210000002966 serum Anatomy 0.000 description 3
239000013589 supplement Substances 0.000 description 3
206010035226 Plasma cell myeloma Diseases 0.000 description 2
230000001464 adherent effect Effects 0.000 description 2
239000000470 constituent Substances 0.000 description 2
238000011109 contamination Methods 0.000 description 2
239000013505 freshwater Substances 0.000 description 2
239000003102 growth factor Substances 0.000 description 2
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
NPFOYSMITVOQOS-UHFFFAOYSA-K iron(III) citrate Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 description 2
238000000034 method Methods 0.000 description 2
201000000050 myeloid neoplasm Diseases 0.000 description 2
230000000644 propagated effect Effects 0.000 description 2
238000004114 suspension culture Methods 0.000 description 2
JVXHQHGWBAHSSF-UHFFFAOYSA-L 2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate;hydron;iron(2+) Chemical compound [H+].[H+].[Fe+2].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O JVXHQHGWBAHSSF-UHFFFAOYSA-L 0.000 description 1
FOPMDZJEFHUNBD-UHFFFAOYSA-N 2-oxidanylpropane-1,2,3-tricarboxylic acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O FOPMDZJEFHUNBD-UHFFFAOYSA-N 0.000 description 1
FZIPCQLKPTZZIM-UHFFFAOYSA-N 2-oxidanylpropane-1,2,3-tricarboxylic acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O FZIPCQLKPTZZIM-UHFFFAOYSA-N 0.000 description 1
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
241000518994 Conta Species 0.000 description 1
102000004877 Insulin Human genes 0.000 description 1
108090001061 Insulin Proteins 0.000 description 1
BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
241000248384 Tetrahymena thermophila Species 0.000 description 1
YJNGWZGWXDHUSH-UHFFFAOYSA-I [Fe+3](Cl)Cl.C(CC(O)(C(=O)[O-])CC(=O)[O-])(=O)[O-] Chemical compound [Fe+3](Cl)Cl.C(CC(O)(C(=O)[O-])CC(=O)[O-])(=O)[O-] YJNGWZGWXDHUSH-UHFFFAOYSA-I 0.000 description 1
GIXWDMTZECRIJT-UHFFFAOYSA-N aurintricarboxylic acid Chemical compound C1=CC(=O)C(C(=O)O)=CC1=C(C=1C=C(C(O)=CC=1)C(O)=O)C1=CC=C(O)C(C(O)=O)=C1 GIXWDMTZECRIJT-UHFFFAOYSA-N 0.000 description 1
239000007640 basal medium Substances 0.000 description 1
239000012620 biological material Substances 0.000 description 1
239000000969 carrier Substances 0.000 description 1
230000010261 cell growth Effects 0.000 description 1
239000002738 chelating agent Substances 0.000 description 1
210000004748 cultured cell Anatomy 0.000 description 1
239000003814 drug Substances 0.000 description 1
230000012010 growth Effects 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
239000012678 infectious agent Substances 0.000 description 1
229940125396 insulin Drugs 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
239000000797 iron chelating agent Substances 0.000 description 1
159000000014 iron salts Chemical class 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
230000003204 osmotic effect Effects 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
239000000047 product Substances 0.000 description 1
238000000746 purification Methods 0.000 description 1
229910052711 selenium Inorganic materials 0.000 description 1
239000011669 selenium Substances 0.000 description 1
238000003756 stirring Methods 0.000 description 1
239000000126 substance Substances 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/005—Protein-free medium
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin

Landscapes

Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Cell Biology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

CA 2111984 1991-06-21 1992-06-18 Additif d'un milieu de culture contenant un agent chelateur du fer Abandoned CA2111984A1 (fr)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP91610054.8		1991-06-21
EP91610054		1991-06-21

Publications (1)

Publication Number	Publication Date
CA2111984A1 true CA2111984A1 (fr)	1993-01-07

Family

ID=8208780

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA 2111984 Abandoned CA2111984A1 (fr)	1991-06-21	1992-06-18	Additif d'un milieu de culture contenant un agent chelateur du fer

Country Status (5)

Country	Link
EP (1)	EP0593539A1 (fr)
JP (1)	JPH06508523A (fr)
AU (1)	AU2196892A (fr)
CA (1)	CA2111984A1 (fr)
WO (1)	WO1993000423A1 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP1482031B1 (fr)	1996-08-30	2015-10-28	Life Technologies Corporation	Milieu de culture de cellules de mammifères exempt de sérum et son utilisation
US6767741B1 (en)	1999-08-27	2004-07-27	Invitrogen Corporation	Metal binding compounds and their use in cell culture medium compositions
WO2001016294A2 (fr) *	1999-08-27	2001-03-08	Invitrogen Corporation	Composes de liaison metallique et leur utilisation dans des compositions de milieu de culture cellulaire
US20030096414A1 (en) *	2001-03-27	2003-05-22	Invitrogen Corporation	Culture medium for cell growth and transfection
CA2417689C (fr)	2002-03-05	2006-05-09	F. Hoffmann-La Roche Ag	Methodes ameliorees de culture in vitro de cellules de mammiferes
GB2404665B (en)	2003-08-08	2005-07-06	Cambridge Antibody Tech	Cell culture
KR101050176B1 (ko) *	2003-08-08	2011-07-19	메디뮨 리미티드	트랜스페린 무함유 및 저농도의 철 함유 배지 중에서의골수종 세포 배양
EP1812557A4 (fr)	2004-10-29	2009-11-04	Centocor Ortho Biotech Inc	Compositions de milieux chimiquement definis
JP5431361B2 (ja)	2008-01-09	2014-03-05	セルカゲーエムベーハー	改善された培養培地添加物及びそれを用いる方法
US20140322758A1 (en) *	2011-10-21	2014-10-30	Pfizer Inc.	Addition of iron to improve cell culture
EP3778868B1 (fr) *	2019-08-16	2022-01-26	UGA Biopharma GmbH	Milieu de culture cellulaire permettant de cultiver des cellules, procédé de culture des cellules et procédé d'expression d'au moins une protéine recombinant dans une culture cellulaire

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CS262822B1 (en) *	1986-10-03	1989-04-14	Kovar Jan	Synthetic medium for the cultivation of myelomic cells
NO162160C (no) *	1987-01-09	1989-11-15	Medi Cult As	Serumfritt vekstmedium, samt anvendelse derav.

1992
- 1992-06-18 EP EP92913614A patent/EP0593539A1/fr not_active Withdrawn
- 1992-06-18 JP JP5501299A patent/JPH06508523A/ja active Pending
- 1992-06-18 WO PCT/DK1992/000190 patent/WO1993000423A1/fr not_active Ceased
- 1992-06-18 CA CA 2111984 patent/CA2111984A1/fr not_active Abandoned
- 1992-06-18 AU AU21968/92A patent/AU2196892A/en not_active Abandoned

Also Published As

Publication number	Publication date
EP0593539A1 (fr)	1994-04-27
JPH06508523A (ja)	1994-09-29
AU2196892A (en)	1993-01-25
WO1993000423A1 (fr)	1993-01-07

Legal Events

Date	Code	Title	Description
1998-06-18	FZDE	Discontinued
2023-10-24	FZDE	Discontinued	Effective date: 19980618

Publication	Publication Date	Title
Yang et al.	2012	Culture conditions and types
Eibl et al.	2008	Cell and tissue reaction engineering
Prasad et al.	1981	Vitamin K3 (menadione) inhibits the growth of mammalian tumor cells in culture
US20130183714A1 (en)	2013-07-18	Myeloma cell culture in transferrin-free low iron medium
JPH04501660A (ja)	1992-03-26	増強された細胞増殖、培養寿命および生産物発現のための細胞培養培地
JPH06511389A (ja)	1994-12-22	動物細胞培養
US20110183375A1 (en)	2011-07-28	Metal binding compounds and their use in cell culture medium compositions
JP6990659B2 (ja)	2022-01-12	がん幹細胞（ｃｓｃ）含有細胞集団の培養のための化学的に規定された培地
CN108925137A (zh)	2018-11-30	细胞培养基
CA2111984A1 (fr)	1993-01-07	Additif d'un milieu de culture contenant un agent chelateur du fer
CA2383460C (fr)	2015-10-13	Composes de liaison metallique et leur utilisation dans des compositions de milieu de culture cellulaire
CN1962857A (zh)	2007-05-16	一种哺乳动物细胞无血清培养基
Minamoto et al.	1991	Development of a serum-free and heat-sterilizable medium and continuous high-density cell culture
CN109370985A (zh)	2019-02-22	一种人脐带间充质干细胞大规模培养无血清培养基
CN106399224A (zh)	2017-02-15	无血清无蛋白细胞培养基
Schumpp et al.	1990	Optimization of culture conditions for high cell density proliferation of HL-60 human promyelocytic leukemia cells
Yabe et al.	1987	Role of iron chelators in growth-promoting effect on mouse hybridoma cells in a chemically defined medium
JP2859679B2 (ja)	1999-02-17	新規細胞株
KR101050176B1 (ko)	2011-07-19	트랜스페린 무함유 및 저농도의 철 함유 배지 중에서의골수종 세포 배양
WO1988001644A1 (fr)	1988-03-10	Procede d'isolement de produits secretes par une tumeur en utilisant un nouveau milieu exempt de proteines
Pörtner	2009	Characteristics of mammalian cells and requirements for cultivation
US20120264208A1 (en)	2012-10-18	Materials and methods for enhanced iron uptake in cell culture
US5602025A (en)	1997-02-11	Non-tumorigenic cell lines for expression of genes
JP2850430B2 (ja)	1999-01-27	細胞培養用無血清培地
JPS6434282A (en)	1989-02-03	Synthetic culture medium for producing monoclonal antibody