JPH06508523A - 鉄キレート培地添加剤 - Google Patents

鉄キレート培地添加剤

Info

Publication number
JPH06508523A
JPH06508523A JP5501299A JP50129993A JPH06508523A JP H06508523 A JPH06508523 A JP H06508523A JP 5501299 A JP5501299 A JP 5501299A JP 50129993 A JP50129993 A JP 50129993A JP H06508523 A JPH06508523 A JP H06508523A
Authority
JP
Japan
Prior art keywords
citrate
medium
iron
additive
alkaline earth
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP5501299A
Other languages
English (en)
Japanese (ja)
Inventor
シュール イェッセン,ペテル ベルント
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of JPH06508523A publication Critical patent/JPH06508523A/ja
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/005Protein-free medium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP5501299A 1991-06-21 1992-06-18 鉄キレート培地添加剤 Pending JPH06508523A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP91610054 1991-06-21
DK91610054.8 1991-06-21
PCT/DK1992/000190 WO1993000423A1 (fr) 1991-06-21 1992-06-18 Additif pour milieu de culture a base de chelate de fer

Publications (1)

Publication Number Publication Date
JPH06508523A true JPH06508523A (ja) 1994-09-29

Family

ID=8208780

Family Applications (1)

Application Number Title Priority Date Filing Date
JP5501299A Pending JPH06508523A (ja) 1991-06-21 1992-06-18 鉄キレート培地添加剤

Country Status (5)

Country Link
EP (1) EP0593539A1 (fr)
JP (1) JPH06508523A (fr)
AU (1) AU2196892A (fr)
CA (1) CA2111984A1 (fr)
WO (1) WO1993000423A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010000094A (ja) * 2002-03-05 2010-01-07 F Hoffmann La Roche Ag 哺乳類細胞をインビトロで増殖させる改善法
JP2014530620A (ja) * 2011-10-21 2014-11-20 ファイザー・インク 細胞培養を改善するための鉄の添加

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4330597A (en) 1996-08-30 1998-03-19 Life Technologies, Inc. Serum-free mammalian cell culture medium, and uses thereof
US6767741B1 (en) 1999-08-27 2004-07-27 Invitrogen Corporation Metal binding compounds and their use in cell culture medium compositions
DE60037819T2 (de) * 1999-08-27 2009-01-08 Invitrogen Corp., Carlsbad Metallbindende verbindungen und deren verwendung in zusammensetzungen für zellkulturmedien
US20030096414A1 (en) 2001-03-27 2003-05-22 Invitrogen Corporation Culture medium for cell growth and transfection
ATE359359T1 (de) * 2003-08-08 2007-05-15 Cambridge Antibody Tech Myeloma zellkultur in einem transferrin-freien medium mit niedrigem eisengehalt
GB2404665B (en) 2003-08-08 2005-07-06 Cambridge Antibody Tech Cell culture
AU2005302516A1 (en) 2004-10-29 2006-05-11 Centocor, Inc. Chemically defined media compositions
KR101594046B1 (ko) 2008-01-09 2016-02-15 첼카 게엠베하 개선된 배양 배지 첨가제 및 이를 이용한 방법
EP3778868B1 (fr) * 2019-08-16 2022-01-26 UGA Biopharma GmbH Milieu de culture cellulaire permettant de cultiver des cellules, procédé de culture des cellules et procédé d'expression d'au moins une protéine recombinant dans une culture cellulaire

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CS262822B1 (en) * 1986-10-03 1989-04-14 Kovar Jan Synthetic medium for the cultivation of myelomic cells
NO162160C (no) * 1987-01-09 1989-11-15 Medi Cult As Serumfritt vekstmedium, samt anvendelse derav.

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010000094A (ja) * 2002-03-05 2010-01-07 F Hoffmann La Roche Ag 哺乳類細胞をインビトロで増殖させる改善法
JP2014530620A (ja) * 2011-10-21 2014-11-20 ファイザー・インク 細胞培養を改善するための鉄の添加

Also Published As

Publication number Publication date
AU2196892A (en) 1993-01-25
EP0593539A1 (fr) 1994-04-27
WO1993000423A1 (fr) 1993-01-07
CA2111984A1 (fr) 1993-01-07

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